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The map label for this gene is zraS [H]

Identifier: 116620567

GI number: 116620567

Start: 1777134

End: 1777901

Strand: Direct

Name: zraS [H]

Synonym: Acid_1445

Alternate gene names: 116620567

Gene position: 1777134-1777901 (Clockwise)

Preceding gene: 116620566

Following gene: 116620568

Centisome position: 17.83

GC content: 61.46

Gene sequence:

>768_bases
ATGATCGTCGAGATTCCCGAAGAACAGCTTCTCGAACTCCAGCGTCAGGCCGAACTCGGCCGTCTCCTCGCCGGAGTGGC
CCACGAGTTCAGCGCGCCAGCCGGTTCCATCCTTTCCAACCGCGACCTCTGCCTGCGCCTGCTCGATCGCATCGAGAAAT
CGATTACGGATTCCGCGACCGCGCGCACCGCCGAACTGCTGACAGCCGCCCGCGAACTGGCGCGCGTGGACCAGATTGCC
GCCGAACGCATCGGTCACCTCGTCCGCAATTTGAAAGTAGCTTCCCGCGTGGCCGATCCCGAACCGCAGCGCGCCAACCT
CAACGAAATCGTCGAGAGTGCAGTCCAACTCGGCAAGACTCAATTCCGTGATCGCATCACCCTGGAAATGGCCTTAGGAC
CCTTGCCCGAAGTGGAGTGCTATCCCCACTTGCTGATCCAGGCGGTCCTGAACCTGGTCGTCAACGCCGGCCAGGCCATC
GAGGGAACCGGCACGATTACTGTCGGCACCCGGCTGGATGGCGACACGATCCACATCTGGGTCTCCGATACGGGCCGCGG
AATCCGCGATCAGGACCAGCCCAAAATCCTGAAGCAGGGTTTCACCACCAAGCCGGTCGGAGTCGGCACCGGCCTCGGCC
TCCTGATGGTACGGCGCGCCGTGGTTGATGACCATGGCGGCTCCATCGCTTTCGAGAGCCAGTGCGGCCACGGAACTACC
TTCCATATCAGAATTCCACTGCAACAGAAAAGAAAAGGAGTTGAGTAG

Upstream 100 bases:

>100_bases
CGCGACGACTTCTACGAATTGCTCTCTGATAACGTTGAGATCACGACGGCCGTCTTCGGTACCCTGGTGAAGCGTTTCCG
CAAACTCGTTGGCCAATGAC

Downstream 100 bases:

>100_bases
AGTGCCAGACAATCGCAAGCCGGTCGTGCTGCTCGTGGACGACGAAGATATGATCCTGACCAGCATCCGGACGCTGCTCA
TGCTGGAAGCGGATTACGAA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MIVEIPEEQLLELQRQAELGRLLAGVAHEFSAPAGSILSNRDLCLRLLDRIEKSITDSATARTAELLTAARELARVDQIA
AERIGHLVRNLKVASRVADPEPQRANLNEIVESAVQLGKTQFRDRITLEMALGPLPEVECYPHLLIQAVLNLVVNAGQAI
EGTGTITVGTRLDGDTIHIWVSDTGRGIRDQDQPKILKQGFTTKPVGVGTGLGLLMVRRAVVDDHGGSIAFESQCGHGTT
FHIRIPLQQKRKGVE

Sequences:

>Translated_255_residues
MIVEIPEEQLLELQRQAELGRLLAGVAHEFSAPAGSILSNRDLCLRLLDRIEKSITDSATARTAELLTAARELARVDQIA
AERIGHLVRNLKVASRVADPEPQRANLNEIVESAVQLGKTQFRDRITLEMALGPLPEVECYPHLLIQAVLNLVVNAGQAI
EGTGTITVGTRLDGDTIHIWVSDTGRGIRDQDQPKILKQGFTTKPVGVGTGLGLLMVRRAVVDDHGGSIAFESQCGHGTT
FHIRIPLQQKRKGVE
>Mature_255_residues
MIVEIPEEQLLELQRQAELGRLLAGVAHEFSAPAGSILSNRDLCLRLLDRIEKSITDSATARTAELLTAARELARVDQIA
AERIGHLVRNLKVASRVADPEPQRANLNEIVESAVQLGKTQFRDRITLEMALGPLPEVECYPHLLIQAVLNLVVNAGQAI
EGTGTITVGTRLDGDTIHIWVSDTGRGIRDQDQPKILKQGFTTKPVGVGTGLGLLMVRRAVVDDHGGSIAFESQCGHGTT
FHIRIPLQQKRKGVE

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=251, Percent_Identity=34.6613545816733, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788549, Length=248, Percent_Identity=32.258064516129, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI145693157, Length=270, Percent_Identity=29.2592592592593, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 27776; Mature: 27776

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVEIPEEQLLELQRQAELGRLLAGVAHEFSAPAGSILSNRDLCLRLLDRIEKSITDSAT
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARTAELLTAARELARVDQIAAERIGHLVRNLKVASRVADPEPQRANLNEIVESAVQLGKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QFRDRITLEMALGPLPEVECYPHLLIQAVLNLVVNAGQAIEGTGTITVGTRLDGDTIHIW
HHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEE
VSDTGRGIRDQDQPKILKQGFTTKPVGVGTGLGLLMVRRAVVDDHGGSIAFESQCGHGTT
ECCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCE
FHIRIPLQQKRKGVE
EEEECCHHHHCCCCC
>Mature Secondary Structure
MIVEIPEEQLLELQRQAELGRLLAGVAHEFSAPAGSILSNRDLCLRLLDRIEKSITDSAT
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARTAELLTAARELARVDQIAAERIGHLVRNLKVASRVADPEPQRANLNEIVESAVQLGKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
QFRDRITLEMALGPLPEVECYPHLLIQAVLNLVVNAGQAIEGTGTITVGTRLDGDTIHIW
HHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEE
VSDTGRGIRDQDQPKILKQGFTTKPVGVGTGLGLLMVRRAVVDDHGGSIAFESQCGHGTT
ECCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCE
FHIRIPLQQKRKGVE
EEEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]