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The map label for this gene is hupR1 [H]

Identifier: 116620568

GI number: 116620568

Start: 1777903

End: 1778382

Strand: Direct

Name: hupR1 [H]

Synonym: Acid_1446

Alternate gene names: 116620568

Gene position: 1777903-1778382 (Clockwise)

Preceding gene: 116620567

Following gene: 116620569

Centisome position: 17.84

GC content: 58.96

Gene sequence:

>480_bases
GTGCCAGACAATCGCAAGCCGGTCGTGCTGCTCGTGGACGACGAAGATATGATCCTGACCAGCATCCGGACGCTGCTCAT
GCTGGAAGCGGATTACGAAGTGGTCTGCTTCACCAATCCCGCCGAGGCCGCCCGCCACCTGGAAAACAATCAGGTGGATA
TCACCGTGTCGGACTACATGATGCCCGGCTTGAACGGCATCCAACTCCTCGGCGAAGCCAAGCGGCTCCAGCCCGAAGCC
ACCCGCGTCCTTCTCACCGGCCACGCCGACAAAGCCAGCGCCATCCAGGCCATCAATCAGGTCGCGCTCTTCCAATACCT
CGAAAAGCCCTGGGACAACGCTCAACTTCTGCTCGTGATCCAGCAGGGAATCGAGCGTGCCCAACTATTCCGCGAACTTC
GTCAGAAGATCATGGAATTGGATACCGCCAACTCCACGCTAAAAGGCACCCAGGCCCGCCTCCTCAAAGCCTTCCTGTAA

Upstream 100 bases:

>100_bases
GACCATGGCGGCTCCATCGCTTTCGAGAGCCAGTGCGGCCACGGAACTACCTTCCATATCAGAATTCCACTGCAACAGAA
AAGAAAAGGAGTTGAGTAGA

Downstream 100 bases:

>100_bases
GACAGGCCCCCCGGCCTGTCCAGTTGCTGCCGTAACTCTCGCGTGCCTGTCGCCTCCACCTCGCCGCCCCCAAGAATTTA
TTAAATCCGATCGAGACGAA

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MPDNRKPVVLLVDDEDMILTSIRTLLMLEADYEVVCFTNPAEAARHLENNQVDITVSDYMMPGLNGIQLLGEAKRLQPEA
TRVLLTGHADKASAIQAINQVALFQYLEKPWDNAQLLLVIQQGIERAQLFRELRQKIMELDTANSTLKGTQARLLKAFL

Sequences:

>Translated_159_residues
MPDNRKPVVLLVDDEDMILTSIRTLLMLEADYEVVCFTNPAEAARHLENNQVDITVSDYMMPGLNGIQLLGEAKRLQPEA
TRVLLTGHADKASAIQAINQVALFQYLEKPWDNAQLLLVIQQGIERAQLFRELRQKIMELDTANSTLKGTQARLLKAFL
>Mature_158_residues
PDNRKPVVLLVDDEDMILTSIRTLLMLEADYEVVCFTNPAEAARHLENNQVDITVSDYMMPGLNGIQLLGEAKRLQPEAT
RVLLTGHADKASAIQAINQVALFQYLEKPWDNAQLLLVIQQGIERAQLFRELRQKIMELDTANSTLKGTQARLLKAFL

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=124, Percent_Identity=32.258064516129, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=31.496062992126, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1788550, Length=135, Percent_Identity=27.4074074074074, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 17931; Mature: 17800

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNRKPVVLLVDDEDMILTSIRTLLMLEADYEVVCFTNPAEAARHLENNQVDITVSDYM
CCCCCCCEEEEECCCCHHHHHHHHHHHEECCCEEEEECCHHHHHHHCCCCEEEEEECCCC
MPGLNGIQLLGEAKRLQPEATRVLLTGHADKASAIQAINQVALFQYLEKPWDNAQLLLVI
CCCCCHHHHHHHHHHCCCCHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHH
QQGIERAQLFRELRQKIMELDTANSTLKGTQARLLKAFL
HHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PDNRKPVVLLVDDEDMILTSIRTLLMLEADYEVVCFTNPAEAARHLENNQVDITVSDYM
CCCCCCEEEEECCCCHHHHHHHHHHHEECCCEEEEECCHHHHHHHCCCCEEEEEECCCC
MPGLNGIQLLGEAKRLQPEATRVLLTGHADKASAIQAINQVALFQYLEKPWDNAQLLLVI
CCCCCHHHHHHHHHHCCCCHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEHHH
QQGIERAQLFRELRQKIMELDTANSTLKGTQARLLKAFL
HHCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]