Click here to switch to the map view.

The map label for this gene is iscS [H]

Identifier: 116620338

GI number: 116620338

Start: 1499667

End: 1500827

Strand: Direct

Name: iscS [H]

Synonym: Acid_1215

Alternate gene names: 116620338

Gene position: 1499667-1500827 (Clockwise)

Preceding gene: 116620333

Following gene: 116620339

Centisome position: 15.05

GC content: 64.17

Gene sequence:

>1161_bases
GTGACAAAAACCATCTATCTTGATTACCACGCGACTACCCCTGTCGATCCGCGCGTGCTGAATGTGATGCTGCCGTTTTT
CGGAGCCAGATTCGGCAACGCCGCCAGCACCAGCCATAGCTATGGGTGGGAGGCGGAGGGCGCGGTGGAAGTGGCGAGGA
AGCGCGTGGCGGCCCTGGCAGGCGCCGCGGCACACGAAATCGTATTCACCAGCGGCGCGACTGAATCAAACAATCTGGCG
ATAAAGGGCGCATTAATGGCCGCCGGCGTCCGCGGCGGTCACATCGTCACGGTCGCCACCGAACACAAGGCGGTGCTGGA
TACGGCCGAGCGGCTGGCGGAAGGGGGCTGCGGGGTCACGATTCTGGCGCCGCGCGCCGATGGCCTGATTGACCTGGACG
AATTGCATGATGCGATTCTCCCCCACACGGTGGTGGTGAGTGTGATGTACGCCAATAACGAAATCGGCGTGATCCAGCCG
GTGCGGCAGATTGGGGCGATTTGCCGCGGGAAGCAGGTGCTGTTCCACTGCGATGCAGTACAGGCGTTCGGAAAAATCCC
GGTAGACGTGGATGCCGACCAAATCGACCTGATGTCGATGAGCGCGCATAAAATGTATGGCCCGAAGGGAGTCGGCGCAC
TCTACGTGCGGCGGCGCAATCCGCGGCTGCACCTGGTTCCGCAGATGGATGGCGGGGGACACGAGGGCGGGCTGCGCTCG
GGGACGTTGAACGTGCCGGGGATTGCGGGCTTCGGCGAAGCCTGCGCGATCGCGGCGGTGGAGATGGGAGCCGAATGCGA
GCGGCTGAGGGGGTTGCGCGACCGGTTGAAGCGGCGCCTGGAGGAAGGCATTCCGGGCTTGCACGTGAACGGGTCGATGG
AACACCGGCTGACAGGCAACCTGAACGTGAGCTTCGAAGGGGTGGAGGGAGATGCTCTGCTGGTCGCGCTGCCGGAACTG
GCGGTTTCGGCGGGTTCAGCGTGCAATTCGCATGAAGGGGGGGCCAGCCACGTGCTCAAGGCGATCGGCCTGAGTCCGGC
GCTGATACAGTCGGCCACGCGCTTTGGCCTGGGCCGTTTCACGACGGAAGAGGAAATCGAGTACGCGGCGGGGCGGGTGG
TGGAGGTTGTGAGCAAACTGCGCGCGGATGCCCCGGTGTGA

Upstream 100 bases:

>100_bases
AAGACCTTGAACAGACGAATCATCTATGGGGACCGAGAAGCGCAAGGTTAATTATACTCGTAGTTGAGAGCGTTCCCAAT
CCTGCATTACCGGGCGTACT

Downstream 100 bases:

>100_bases
GGGCGAAGGGACGAATTGCGGACGCGGCTCGCTTCGAAATATCGAAATTTATTTGAGCGATACCTCAGCGTTTGTCAATA
AGTTACCAGGCTTTGCCGTC

Product: class V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTKTIYLDYHATTPVDPRVLNVMLPFFGARFGNAASTSHSYGWEAEGAVEVARKRVAALAGAAAHEIVFTSGATESNNLA
IKGALMAAGVRGGHIVTVATEHKAVLDTAERLAEGGCGVTILAPRADGLIDLDELHDAILPHTVVVSVMYANNEIGVIQP
VRQIGAICRGKQVLFHCDAVQAFGKIPVDVDADQIDLMSMSAHKMYGPKGVGALYVRRRNPRLHLVPQMDGGGHEGGLRS
GTLNVPGIAGFGEACAIAAVEMGAECERLRGLRDRLKRRLEEGIPGLHVNGSMEHRLTGNLNVSFEGVEGDALLVALPEL
AVSAGSACNSHEGGASHVLKAIGLSPALIQSATRFGLGRFTTEEEIEYAAGRVVEVVSKLRADAPV

Sequences:

>Translated_386_residues
MTKTIYLDYHATTPVDPRVLNVMLPFFGARFGNAASTSHSYGWEAEGAVEVARKRVAALAGAAAHEIVFTSGATESNNLA
IKGALMAAGVRGGHIVTVATEHKAVLDTAERLAEGGCGVTILAPRADGLIDLDELHDAILPHTVVVSVMYANNEIGVIQP
VRQIGAICRGKQVLFHCDAVQAFGKIPVDVDADQIDLMSMSAHKMYGPKGVGALYVRRRNPRLHLVPQMDGGGHEGGLRS
GTLNVPGIAGFGEACAIAAVEMGAECERLRGLRDRLKRRLEEGIPGLHVNGSMEHRLTGNLNVSFEGVEGDALLVALPEL
AVSAGSACNSHEGGASHVLKAIGLSPALIQSATRFGLGRFTTEEEIEYAAGRVVEVVSKLRADAPV
>Mature_385_residues
TKTIYLDYHATTPVDPRVLNVMLPFFGARFGNAASTSHSYGWEAEGAVEVARKRVAALAGAAAHEIVFTSGATESNNLAI
KGALMAAGVRGGHIVTVATEHKAVLDTAERLAEGGCGVTILAPRADGLIDLDELHDAILPHTVVVSVMYANNEIGVIQPV
RQIGAICRGKQVLFHCDAVQAFGKIPVDVDADQIDLMSMSAHKMYGPKGVGALYVRRRNPRLHLVPQMDGGGHEGGLRSG
TLNVPGIAGFGEACAIAAVEMGAECERLRGLRDRLKRRLEEGIPGLHVNGSMEHRLTGNLNVSFEGVEGDALLVALPELA
VSAGSACNSHEGGASHVLKAIGLSPALIQSATRFGLGRFTTEEEIEYAAGRVVEVVSKLRADAPV

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=384, Percent_Identity=49.7395833333333, Blast_Score=386, Evalue=1e-107,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=32.2966507177034, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI48994898, Length=388, Percent_Identity=53.6082474226804, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1787970, Length=308, Percent_Identity=27.9220779220779, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789175, Length=348, Percent_Identity=26.7241379310345, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=385, Percent_Identity=49.3506493506493, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17533177, Length=323, Percent_Identity=34.6749226006192, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=387, Percent_Identity=51.421188630491, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI20129463, Length=384, Percent_Identity=50.5208333333333, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40641; Mature: 40510

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIYLDYHATTPVDPRVLNVMLPFFGARFGNAASTSHSYGWEAEGAVEVARKRVAALA
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GAAAHEIVFTSGATESNNLAIKGALMAAGVRGGHIVTVATEHKAVLDTAERLAEGGCGVT
CCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEE
ILAPRADGLIDLDELHDAILPHTVVVSVMYANNEIGVIQPVRQIGAICRGKQVLFHCDAV
EEECCCCCCEEHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEHHH
QAFGKIPVDVDADQIDLMSMSAHKMYGPKGVGALYVRRRNPRLHLVPQMDGGGHEGGLRS
HHHCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCC
GTLNVPGIAGFGEACAIAAVEMGAECERLRGLRDRLKRRLEEGIPGLHVNGSMEHRLTGN
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECC
LNVSFEGVEGDALLVALPELAVSAGSACNSHEGGASHVLKAIGLSPALIQSATRFGLGRF
EEEEEECCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
TTEEEIEYAAGRVVEVVSKLRADAPV
CCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TKTIYLDYHATTPVDPRVLNVMLPFFGARFGNAASTSHSYGWEAEGAVEVARKRVAALA
CEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GAAAHEIVFTSGATESNNLAIKGALMAAGVRGGHIVTVATEHKAVLDTAERLAEGGCGVT
CCCCEEEEEECCCCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEE
ILAPRADGLIDLDELHDAILPHTVVVSVMYANNEIGVIQPVRQIGAICRGKQVLFHCDAV
EEECCCCCCEEHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEEHHH
QAFGKIPVDVDADQIDLMSMSAHKMYGPKGVGALYVRRRNPRLHLVPQMDGGGHEGGLRS
HHHCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCCCCCCCCC
GTLNVPGIAGFGEACAIAAVEMGAECERLRGLRDRLKRRLEEGIPGLHVNGSMEHRLTGN
CCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECC
LNVSFEGVEGDALLVALPELAVSAGSACNSHEGGASHVLKAIGLSPALIQSATRFGLGRF
EEEEEECCCCCEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
TTEEEIEYAAGRVVEVVSKLRADAPV
CCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA