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The map label for this gene is wcaJ [H]

Identifier: 116620337

GI number: 116620337

Start: 1498198

End: 1499589

Strand: Reverse

Name: wcaJ [H]

Synonym: Acid_1214

Alternate gene names: 116620337

Gene position: 1499589-1498198 (Counterclockwise)

Preceding gene: 116620346

Following gene: 116620336

Centisome position: 15.05

GC content: 60.34

Gene sequence:

>1392_bases
ATGATTCGTCTGTTCAAGGTCTTCATTCCAGTTGGAACACTGACGCTGCTGGTTTCAGAAGCCGTTCTCGTGACCTCGGC
GTTCATTTTCGCGACGTACCTGGCGCTGGAAGTGGATCCGACCGTTTTCCTGCTTTACGATGGCGGACTGACGAGAATTC
TGTTCGTGCTCCTCAGCATTCTGATCGGGCTGCATTTCCACGATCTGTATTCGCAATTTCACGTTAAGTCTCGCATCGTG
CTGGTGCAGCAGCTCTGCCTCGTAATGGGCGTGGCCTTCCTCACCCAGGGCCTGATCGCCTATCTCAACGCCGGTCTGCG
CGTACCAATCCGCGTGATGGTCGTGGGCAGCGGCCTCGCAGTTATCTTGATTTTCTTCTGGCGGGTCTTCTTCAGCGCCT
ACGCCCTCCAGGTGGTGGGGCGCGACCGCCTGCTGCTGGTGGGCGGCAGTCCCCTGCTCGAGGACATCGCGAAACACGTC
GTTGACCATCCGGAGGCTGGGCTGATGATCGCCGGATATGTGGACGATCGCCACCAGGTCGATCAGTCCCTCCCCGGCGG
CAAGAGTATCGGAAACCTCTCCCAGTTGCGCGACATCGTGCGCGCCACGCAGCCCCACCGCATCGTGGTTGGCATGTTCG
AACGCCGCAACCGCATGCCTATGGGCGACCTGCTCGAGCTCCGCTTCGCCGGCCACATCATCGAAGAGGTCGCCAACACG
TATGAACGCGTCTGCGGCCGCGTCTGCCTCAAAGAGATCCGCCCGTCACAACTGATCTTCTCGGGAGAACTCGGACCGCG
CCCGCAAAACCTGATCTATCAACGGATGTCCAACATGGCCGTCGCGGTGATTGGAATCGTCCTCGCCGCGCCCATCATGC
TACTGACCGCTCTCGCGGTCAGACTCTCTTCGCCAGGCCCGGTGCTCTACCGGCAAACCCGCGTGGGCCTGGACGATTCC
CTCTTCACCGTCTACAAATTCCGCTCCATGCGGGTGGATGCCGAAGCCGCCACGGGTGCCGTCTGGGCGCAGAAGGACGA
TCCCCGCGTAACGTCAGTGGGCCGTATCATCCGCCGCATCCGCTTCGACGAGCTGCCCCAGTTGTTTAACGTGCTGAAGG
GCGAGATGTCGATGGTCGGCCCGCGCCCGGAGCGCCCCGAATTCGTCAAGGCGCTCAGCGAACAGATCCCCTATTACCGC
CAGCGCCACTGCGTCCGGCCGGGCATCACCGGATGGGCGCAGATCAACTACAAATACGGGGACACTCTGGAAGACACCAT
CACCAAGCTGGAGTACGACCTGTACTACATCAAGAACATGTCCATGGCGCTGGACAACTACATCATTTTTCATACCCTCA
AAGCCATGCTGTTGTCGCGCGGCGCGCAATAG

Upstream 100 bases:

>100_bases
TCAAGATAGATGGTTTTTGTCACAGTACGCCCGGTAATGCAGGATTGGGAACGCTCTCAACTACGAGTATAATTAACCTT
GCGCTTCTCGGTCCCCATAG

Downstream 100 bases:

>100_bases
AGCATACTGTTCAATGCGGCGGCATGCCGCTCAAATGGCAAACCCCGCGATTGCCCCGGACACCGAGATTCTGGAACGGG
AACGGGAGCAGCACGTCCCG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MIRLFKVFIPVGTLTLLVSEAVLVTSAFIFATYLALEVDPTVFLLYDGGLTRILFVLLSILIGLHFHDLYSQFHVKSRIV
LVQQLCLVMGVAFLTQGLIAYLNAGLRVPIRVMVVGSGLAVILIFFWRVFFSAYALQVVGRDRLLLVGGSPLLEDIAKHV
VDHPEAGLMIAGYVDDRHQVDQSLPGGKSIGNLSQLRDIVRATQPHRIVVGMFERRNRMPMGDLLELRFAGHIIEEVANT
YERVCGRVCLKEIRPSQLIFSGELGPRPQNLIYQRMSNMAVAVIGIVLAAPIMLLTALAVRLSSPGPVLYRQTRVGLDDS
LFTVYKFRSMRVDAEAATGAVWAQKDDPRVTSVGRIIRRIRFDELPQLFNVLKGEMSMVGPRPERPEFVKALSEQIPYYR
QRHCVRPGITGWAQINYKYGDTLEDTITKLEYDLYYIKNMSMALDNYIIFHTLKAMLLSRGAQ

Sequences:

>Translated_463_residues
MIRLFKVFIPVGTLTLLVSEAVLVTSAFIFATYLALEVDPTVFLLYDGGLTRILFVLLSILIGLHFHDLYSQFHVKSRIV
LVQQLCLVMGVAFLTQGLIAYLNAGLRVPIRVMVVGSGLAVILIFFWRVFFSAYALQVVGRDRLLLVGGSPLLEDIAKHV
VDHPEAGLMIAGYVDDRHQVDQSLPGGKSIGNLSQLRDIVRATQPHRIVVGMFERRNRMPMGDLLELRFAGHIIEEVANT
YERVCGRVCLKEIRPSQLIFSGELGPRPQNLIYQRMSNMAVAVIGIVLAAPIMLLTALAVRLSSPGPVLYRQTRVGLDDS
LFTVYKFRSMRVDAEAATGAVWAQKDDPRVTSVGRIIRRIRFDELPQLFNVLKGEMSMVGPRPERPEFVKALSEQIPYYR
QRHCVRPGITGWAQINYKYGDTLEDTITKLEYDLYYIKNMSMALDNYIIFHTLKAMLLSRGAQ
>Mature_463_residues
MIRLFKVFIPVGTLTLLVSEAVLVTSAFIFATYLALEVDPTVFLLYDGGLTRILFVLLSILIGLHFHDLYSQFHVKSRIV
LVQQLCLVMGVAFLTQGLIAYLNAGLRVPIRVMVVGSGLAVILIFFWRVFFSAYALQVVGRDRLLLVGGSPLLEDIAKHV
VDHPEAGLMIAGYVDDRHQVDQSLPGGKSIGNLSQLRDIVRATQPHRIVVGMFERRNRMPMGDLLELRFAGHIIEEVANT
YERVCGRVCLKEIRPSQLIFSGELGPRPQNLIYQRMSNMAVAVIGIVLAAPIMLLTALAVRLSSPGPVLYRQTRVGLDDS
LFTVYKFRSMRVDAEAATGAVWAQKDDPRVTSVGRIIRRIRFDELPQLFNVLKGEMSMVGPRPERPEFVKALSEQIPYYR
QRHCVRPGITGWAQINYKYGDTLEDTITKLEYDLYYIKNMSMALDNYIIFHTLKAMLLSRGAQ

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=335, Percent_Identity=34.3283582089552, Blast_Score=168, Evalue=6e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52113; Mature: 52113

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLFKVFIPVGTLTLLVSEAVLVTSAFIFATYLALEVDPTVFLLYDGGLTRILFVLLSI
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHHHHH
LIGLHFHDLYSQFHVKSRIVLVQQLCLVMGVAFLTQGLIAYLNAGLRVPIRVMVVGSGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHH
VILIFFWRVFFSAYALQVVGRDRLLLVGGSPLLEDIAKHVVDHPEAGLMIAGYVDDRHQV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHH
DQSLPGGKSIGNLSQLRDIVRATQPHRIVVGMFERRNRMPMGDLLELRFAGHIIEEVANT
HHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
YERVCGRVCLKEIRPSQLIFSGELGPRPQNLIYQRMSNMAVAVIGIVLAAPIMLLTALAV
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSSPGPVLYRQTRVGLDDSLFTVYKFRSMRVDAEAATGAVWAQKDDPRVTSVGRIIRRI
HCCCCCCEEEEHHCCCCCCHHHHHHHHHHHEECCHHHCCCEEECCCCCCHHHHHHHHHHH
RFDELPQLFNVLKGEMSMVGPRPERPEFVKALSEQIPYYRQRHCVRPGITGWAQINYKYG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEECC
DTLEDTITKLEYDLYYIKNMSMALDNYIIFHTLKAMLLSRGAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRLFKVFIPVGTLTLLVSEAVLVTSAFIFATYLALEVDPTVFLLYDGGLTRILFVLLSI
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHHHHH
LIGLHFHDLYSQFHVKSRIVLVQQLCLVMGVAFLTQGLIAYLNAGLRVPIRVMVVGSGLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHH
VILIFFWRVFFSAYALQVVGRDRLLLVGGSPLLEDIAKHVVDHPEAGLMIAGYVDDRHQV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHH
DQSLPGGKSIGNLSQLRDIVRATQPHRIVVGMFERRNRMPMGDLLELRFAGHIIEEVANT
HHCCCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
YERVCGRVCLKEIRPSQLIFSGELGPRPQNLIYQRMSNMAVAVIGIVLAAPIMLLTALAV
HHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSSPGPVLYRQTRVGLDDSLFTVYKFRSMRVDAEAATGAVWAQKDDPRVTSVGRIIRRI
HCCCCCCEEEEHHCCCCCCHHHHHHHHHHHEECCHHHCCCEEECCCCCCHHHHHHHHHHH
RFDELPQLFNVLKGEMSMVGPRPERPEFVKALSEQIPYYRQRHCVRPGITGWAQINYKYG
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEECC
DTLEDTITKLEYDLYYIKNMSMALDNYIIFHTLKAMLLSRGAQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]