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The map label for this gene is rhgT [H]

Identifier: 116620221

GI number: 116620221

Start: 1349014

End: 1350705

Strand: Direct

Name: rhgT [H]

Synonym: Acid_1098

Alternate gene names: 116620221

Gene position: 1349014-1350705 (Clockwise)

Preceding gene: 116620220

Following gene: 116620222

Centisome position: 13.54

GC content: 64.07

Gene sequence:

>1692_bases
ATGATCCCCCGGTTTTGTGTTCTCTTCGCGCTGCTCGCGGCCCCCTGCGGCGCGCAGACGGTGAAGATCGTGCTGGTCGG
CGATTCCACGGTCAATGACGAAGGCGGCTGGGGACCGGGACTCCGCGCCTCCTTCGGACCCGGCGTGCAGGTGGTCAATC
TGGCCCAGAATGGGCGCAGTTCCAAGAGCTTCCGCGACGAGGGCTGGTGGGCCAAAGTGGCGCCGGCAAAACCGAACTTC
GTGCTCATCCAGTTCGGCCACAACGACGTCGCCGGCAAAGGGCCGGAGCGCGAGACCGATCCCGCCACCACCTATCGCGC
CAACATGGAGCGCTACGTGGAAGAGGTGCGGGCCATGGGAGCCACGCCGGTATTGGTGACCTCGATCGTCCGCCGGAACT
TTGACTCACAGGGCAAGTTCCAGCCGGACACACTGGTGCCGTACGCCGAGGCGGTGCGGAGGCTCGCGGTGGAACGGGAA
GTCGCGCTGATCGACCTCTACACGCTCACCCGCGAGCAGGCGGAACGTCTAGGTCCGGCCGGCAGCGAGCAGTTGGGTCG
GAAAGACCCGGAGGGCAGGCCCGACCACACCCACCTCGGACCCAAAGGCCAGACCGAAATCGGCATCATGGCGGCGCGCG
AACTCGCCAGGGTCGCGCCGCAGGTCGGCGCGTCATTCCATGAGCTGGTGATTTGGCGCAATGCGATGCGGCAGCCGGCG
GCGTGGTACGGCAGCGCCGAGGCCGTGCGCATCGCCGACAACCTATTGGCCTATCAGCGCGACATCGGCGGCTGGGACAA
GAACATCGATATGGCGATGCCGCTCGGAACCCTCGAACGCGCGCAGCTTGAGAAAGAAAAGAGCGACCCCGAGGCGCATT
CCACCATCGATAACGACGCCACCTACACGCAGATGCGCTATCTGGCGAAGGTCTACACCGCCACCAAAGATGCGCGCTTC
GCGGCGGGCTTCCGGCGCGGTTTCGAGTACCTGCTCAAGGCGCAGTATCCCAACGGAGGCTGGCCGCAGTTCTATCCGCT
TCGAGACGGGTATTGGAGCCACATTACCTACAATGACGACGCCATGGTCGGCGTACTGGAAACCCTCCGCGACATCGTGA
CGCGCCGCCCCGACTATGCGTTCCTCGGCGACGCCGAGCGCGCCCGCGCCAAACTCGCCATCGATAAAGGTGTCGAGTGC
ATTCTCAAGACGCAAGTCGTGCAACACGGAAAACTCACCGTCTGGTGCGCACAGCACGATGAGCACTCCCTCGCGCCGGC
CAAGGCGCGCGCCTACGAATTACCCTCCTTGAGCGGTGCCGAAAGCGTTGGCATAGTTCAGTTCCTGATGGGGATCGATA
AGCCATCGCCCGAAGTCGTCAAAGCTGTGGAAGCCGCCGTCGCCTGGTTCCGCGCCTCGAAAATCACCGGAATCCGGATC
GAGCACAGGCCGGCCCCCGGCACGCCGAAAGGCTACGACGATACCGTAGTCGAAGACCCGTCCGCTCCCCCTTTGTGGGC
CCGTTTCTACGAGCTCGGCACCAACCGTCCCATATTTTCAGGCCGCGACTCCGTGGTGAAGTACACCGTGGCGGAGATCG
AATACGAGCGCCGCAACGGCTACCGCTGGTATGTCGATCGCCCCGCCCGCCTCCTCGACATCGATTACCCCGAGTGGCGG
AAAAAACACTAA

Upstream 100 bases:

>100_bases
CTGGCCGACGAGGGGGAGCGGCAGGCGATCATCGAATACCTGCGGCAGATCTCGTCAAAGTAAAGAGGTGGGATAATGGC
CGTTTGGCCGCCTCTATTCT

Downstream 100 bases:

>100_bases
TCAGCCCTGACTGCCGCCTGTTAAAATTGTCATCTCCCGATGCCGTTTTCCCCTCTGCAGCAGCGAGCGGCCACGATCCT
GCTCGCAGTCGCCGGCTTCA

Product: pectate lyase

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MIPRFCVLFALLAAPCGAQTVKIVLVGDSTVNDEGGWGPGLRASFGPGVQVVNLAQNGRSSKSFRDEGWWAKVAPAKPNF
VLIQFGHNDVAGKGPERETDPATTYRANMERYVEEVRAMGATPVLVTSIVRRNFDSQGKFQPDTLVPYAEAVRRLAVERE
VALIDLYTLTREQAERLGPAGSEQLGRKDPEGRPDHTHLGPKGQTEIGIMAARELARVAPQVGASFHELVIWRNAMRQPA
AWYGSAEAVRIADNLLAYQRDIGGWDKNIDMAMPLGTLERAQLEKEKSDPEAHSTIDNDATYTQMRYLAKVYTATKDARF
AAGFRRGFEYLLKAQYPNGGWPQFYPLRDGYWSHITYNDDAMVGVLETLRDIVTRRPDYAFLGDAERARAKLAIDKGVEC
ILKTQVVQHGKLTVWCAQHDEHSLAPAKARAYELPSLSGAESVGIVQFLMGIDKPSPEVVKAVEAAVAWFRASKITGIRI
EHRPAPGTPKGYDDTVVEDPSAPPLWARFYELGTNRPIFSGRDSVVKYTVAEIEYERRNGYRWYVDRPARLLDIDYPEWR
KKH

Sequences:

>Translated_563_residues
MIPRFCVLFALLAAPCGAQTVKIVLVGDSTVNDEGGWGPGLRASFGPGVQVVNLAQNGRSSKSFRDEGWWAKVAPAKPNF
VLIQFGHNDVAGKGPERETDPATTYRANMERYVEEVRAMGATPVLVTSIVRRNFDSQGKFQPDTLVPYAEAVRRLAVERE
VALIDLYTLTREQAERLGPAGSEQLGRKDPEGRPDHTHLGPKGQTEIGIMAARELARVAPQVGASFHELVIWRNAMRQPA
AWYGSAEAVRIADNLLAYQRDIGGWDKNIDMAMPLGTLERAQLEKEKSDPEAHSTIDNDATYTQMRYLAKVYTATKDARF
AAGFRRGFEYLLKAQYPNGGWPQFYPLRDGYWSHITYNDDAMVGVLETLRDIVTRRPDYAFLGDAERARAKLAIDKGVEC
ILKTQVVQHGKLTVWCAQHDEHSLAPAKARAYELPSLSGAESVGIVQFLMGIDKPSPEVVKAVEAAVAWFRASKITGIRI
EHRPAPGTPKGYDDTVVEDPSAPPLWARFYELGTNRPIFSGRDSVVKYTVAEIEYERRNGYRWYVDRPARLLDIDYPEWR
KKH
>Mature_563_residues
MIPRFCVLFALLAAPCGAQTVKIVLVGDSTVNDEGGWGPGLRASFGPGVQVVNLAQNGRSSKSFRDEGWWAKVAPAKPNF
VLIQFGHNDVAGKGPERETDPATTYRANMERYVEEVRAMGATPVLVTSIVRRNFDSQGKFQPDTLVPYAEAVRRLAVERE
VALIDLYTLTREQAERLGPAGSEQLGRKDPEGRPDHTHLGPKGQTEIGIMAARELARVAPQVGASFHELVIWRNAMRQPA
AWYGSAEAVRIADNLLAYQRDIGGWDKNIDMAMPLGTLERAQLEKEKSDPEAHSTIDNDATYTQMRYLAKVYTATKDARF
AAGFRRGFEYLLKAQYPNGGWPQFYPLRDGYWSHITYNDDAMVGVLETLRDIVTRRPDYAFLGDAERARAKLAIDKGVEC
ILKTQVVQHGKLTVWCAQHDEHSLAPAKARAYELPSLSGAESVGIVQFLMGIDKPSPEVVKAVEAAVAWFRASKITGIRI
EHRPAPGTPKGYDDTVVEDPSAPPLWARFYELGTNRPIFSGRDSVVKYTVAEIEYERRNGYRWYVDRPARLLDIDYPEWR
KKH

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 62918; Mature: 62918

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRFCVLFALLAAPCGAQTVKIVLVGDSTVNDEGGWGPGLRASFGPGVQVVNLAQNGRS
CCHHHHHHHHHHHCCCCCCEEEEEEEECCEECCCCCCCCCCCCCCCCCCEEEEECCCCCC
SKSFRDEGWWAKVAPAKPNFVLIQFGHNDVAGKGPERETDPATTYRANMERYVEEVRAMG
CCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
ATPVLVTSIVRRNFDSQGKFQPDTLVPYAEAVRRLAVEREVALIDLYTLTREQAERLGPA
CCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHHHHCCCC
GSEQLGRKDPEGRPDHTHLGPKGQTEIGIMAARELARVAPQVGASFHELVIWRNAMRQPA
CCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
AWYGSAEAVRIADNLLAYQRDIGGWDKNIDMAMPLGTLERAQLEKEKSDPEAHSTIDNDA
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHCCCCHHHHCCCCCH
TYTQMRYLAKVYTATKDARFAAGFRRGFEYLLKAQYPNGGWPQFYPLRDGYWSHITYNDD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCEEECCCCCEEEEEECCC
AMVGVLETLRDIVTRRPDYAFLGDAERARAKLAIDKGVECILKTQVVQHGKLTVWCAQHD
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEEECCC
EHSLAPAKARAYELPSLSGAESVGIVQFLMGIDKPSPEVVKAVEAAVAWFRASKITGIRI
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
EHRPAPGTPKGYDDTVVEDPSAPPLWARFYELGTNRPIFSGRDSVVKYTVAEIEYERRNG
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHEEEEHHHEEECCCCC
YRWYVDRPARLLDIDYPEWRKKH
EEEEEECCCEEEECCCCHHHCCC
>Mature Secondary Structure
MIPRFCVLFALLAAPCGAQTVKIVLVGDSTVNDEGGWGPGLRASFGPGVQVVNLAQNGRS
CCHHHHHHHHHHHCCCCCCEEEEEEEECCEECCCCCCCCCCCCCCCCCCEEEEECCCCCC
SKSFRDEGWWAKVAPAKPNFVLIQFGHNDVAGKGPERETDPATTYRANMERYVEEVRAMG
CCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
ATPVLVTSIVRRNFDSQGKFQPDTLVPYAEAVRRLAVEREVALIDLYTLTREQAERLGPA
CCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHHHHCCCC
GSEQLGRKDPEGRPDHTHLGPKGQTEIGIMAARELARVAPQVGASFHELVIWRNAMRQPA
CCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
AWYGSAEAVRIADNLLAYQRDIGGWDKNIDMAMPLGTLERAQLEKEKSDPEAHSTIDNDA
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHHCCCCHHHHCCCCCH
TYTQMRYLAKVYTATKDARFAAGFRRGFEYLLKAQYPNGGWPQFYPLRDGYWSHITYNDD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEECCCCCCCCCEEECCCCCEEEEEECCC
AMVGVLETLRDIVTRRPDYAFLGDAERARAKLAIDKGVECILKTQVVQHGKLTVWCAQHD
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEEEECCC
EHSLAPAKARAYELPSLSGAESVGIVQFLMGIDKPSPEVVKAVEAAVAWFRASKITGIRI
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
EHRPAPGTPKGYDDTVVEDPSAPPLWARFYELGTNRPIFSGRDSVVKYTVAEIEYERRNG
ECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHEEEEHHHEEECCCCC
YRWYVDRPARLLDIDYPEWRKKH
EEEEEECCCEEEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]