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The map label for this gene is rpoD [H]

Identifier: 116620135

GI number: 116620135

Start: 1254066

End: 1255757

Strand: Reverse

Name: rpoD [H]

Synonym: Acid_1008

Alternate gene names: 116620135

Gene position: 1255757-1254066 (Counterclockwise)

Preceding gene: 116620136

Following gene: 116620133

Centisome position: 12.6

GC content: 60.46

Gene sequence:

>1692_bases
GTGGCGTTAGACGATAAATACGACGGGCTTCAACGGCTCGTACAGATGGGCAAGGAAAAAGGCTACGTCTTGTACGACGA
GGTCAGCGAAGTGCTGCCCGGCGATATCAACGGCGGCAACGATCTTGACGACGTGCTGGCCGGCCTCGATGTCGCGGGAA
TCGAAATCCTGGAAGAACCGAAGGATTTCGAGAAAAAGATCGAAGAGTCCAGTGAAGAATTGATCGATCTGGAATTGCCC
GCCGGGCTGGGCGGCGAGAAGATCAACGATCCCGTTCGCATGTACCTGCGCGAGATGGGAACGGTCCCGCTGCTCACCCG
CGAGGGCGAGGTGGAGATCGCGCGGCGCATCGAGCGCGGGCAAAACACAGTGCTGAAATCGCTCTTCCGGGCGCCCCTGG
TGGTCCAGGAGATCATCGGGATGGGAGAGGAAGTAATCAACGATACGCTTTCCGCCAGGGACGTTATTCAAATCGCCGAC
CCCATGCTCACCGATGAGATGGTGGAGGAGAAGCGCCAGGAATTCGCCCGCCACACCGAAGAGATCGCGCGGTATTACAA
GAAGACGCTGCAGTGCCGGCAGAAGCTGATGGCGATACCGCGCGGCATGAAGCCCAAGCAGTACAGGCGGCAGTTGTGGG
AAGTGGCGCGGCTGATCGTCAGGACTGCCCGCCTGTGCCGCGCCGTCAAATTCCAGTCGCCCGTCATCCGGCATCTGATC
GACCGGCTGCGCAGCGCGGTGGAAGAGTTGCGTCCTGTGGAGAACGATCTCGCCAAGACACAGCGCAAGCTGGAGACGTG
GCCGGCGGCGCGGGCGGAGGCCATTAAGGATCTGCGCAAGGAACAGCGCAGCTACGGCCAGAAATTGCAGCAGATGGAGG
AGCAGTACGGCGCCTCGGCGGCCGAGCTGCGGCGCACGCTCGAAATAATCACGCAGGCGCACCAGGAAGCCGAGACCGCC
AAAAAGGAACTGATCGAAGCAAACCTGCGGCTGGTGGTTAGCATTGCGAAGCGGTATACCAATCGCGGGCTTCAATTCCT
GGATTTGATCCAGGAGGGTAATATCGGCCTGATGAAGGCCGTGGATAAGTTTGAGTACCGGCGCGGCTACAAGTTCTCGA
CGTACGCCACCTGGTGGGTGCGGCAGGCCATCACGCGAGCGATCGCGGACCAGGCGCGCACCATCCGTATCCCGGTGCAC
ATGATCGAGACGATCAATAAGCTGATCCGGACGTCGCGACAGATGGTGCAAGAGCTGGGTCGCGAGCCGAGCAATGAAGA
GCTTGCCAAGCGGCTCCAGTTGCCGGTCTCCAAGGTCAGGAAGGTGCTGAGGGTCGCGCAGGAGCCGATTTCGCTGGAAA
CTCCGATCGGGGAGGAGGAGGAATCGCACCTAGGCGATTTCATCGTCGATCAGAACGGAATTTCACCATCGGATGCGGTG
ATCAACCTGAATCTACGGGAGCAGACGGCGCAGGTGCTGAAGACCCTGACCCCGCGCGAAGAGAAGATCATCAAAATGCG
ATTCGGGCTGGAAGATGGGAGCGAGCACACGCTCGAGGAGGTCGGCCAGAATTTCGCGGTTACCCGCGAGCGCATCCGGC
AGATCGAGGCGAAGGCATTGCGCAAACTCCGCCATCCGAGCCGCAGCCACCGGCTCCGCGCCTTCCTGGAAAACGGAACG
AGGGGCGTCTAG

Upstream 100 bases:

>100_bases
GCTCGCGGGAGGCGGGCATGACAGGATTGCAACCCAATGTGCGTCGTGCAGTGGTGTACAATAATCAGGATTCTTCATCC
GCTGGGAGTACGGGGCTTCA

Downstream 100 bases:

>100_bases
GTTAGACGCCCCTCGTTTTTACTGCTTCTCGAAACCGTTACCAGCGCGGTTCGCGCCGATTGCCGCCGCCGCCGCCACCA
CCGCCTCCAGGCCGACGGCC

Product: RpoD family RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-80 [H]

Number of amino acids: Translated: 563; Mature: 562

Protein sequence:

>563_residues
MALDDKYDGLQRLVQMGKEKGYVLYDEVSEVLPGDINGGNDLDDVLAGLDVAGIEILEEPKDFEKKIEESSEELIDLELP
AGLGGEKINDPVRMYLREMGTVPLLTREGEVEIARRIERGQNTVLKSLFRAPLVVQEIIGMGEEVINDTLSARDVIQIAD
PMLTDEMVEEKRQEFARHTEEIARYYKKTLQCRQKLMAIPRGMKPKQYRRQLWEVARLIVRTARLCRAVKFQSPVIRHLI
DRLRSAVEELRPVENDLAKTQRKLETWPAARAEAIKDLRKEQRSYGQKLQQMEEQYGASAAELRRTLEIITQAHQEAETA
KKELIEANLRLVVSIAKRYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWVRQAITRAIADQARTIRIPVH
MIETINKLIRTSRQMVQELGREPSNEELAKRLQLPVSKVRKVLRVAQEPISLETPIGEEEESHLGDFIVDQNGISPSDAV
INLNLREQTAQVLKTLTPREEKIIKMRFGLEDGSEHTLEEVGQNFAVTRERIRQIEAKALRKLRHPSRSHRLRAFLENGT
RGV

Sequences:

>Translated_563_residues
MALDDKYDGLQRLVQMGKEKGYVLYDEVSEVLPGDINGGNDLDDVLAGLDVAGIEILEEPKDFEKKIEESSEELIDLELP
AGLGGEKINDPVRMYLREMGTVPLLTREGEVEIARRIERGQNTVLKSLFRAPLVVQEIIGMGEEVINDTLSARDVIQIAD
PMLTDEMVEEKRQEFARHTEEIARYYKKTLQCRQKLMAIPRGMKPKQYRRQLWEVARLIVRTARLCRAVKFQSPVIRHLI
DRLRSAVEELRPVENDLAKTQRKLETWPAARAEAIKDLRKEQRSYGQKLQQMEEQYGASAAELRRTLEIITQAHQEAETA
KKELIEANLRLVVSIAKRYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWVRQAITRAIADQARTIRIPVH
MIETINKLIRTSRQMVQELGREPSNEELAKRLQLPVSKVRKVLRVAQEPISLETPIGEEEESHLGDFIVDQNGISPSDAV
INLNLREQTAQVLKTLTPREEKIIKMRFGLEDGSEHTLEEVGQNFAVTRERIRQIEAKALRKLRHPSRSHRLRAFLENGT
RGV
>Mature_562_residues
ALDDKYDGLQRLVQMGKEKGYVLYDEVSEVLPGDINGGNDLDDVLAGLDVAGIEILEEPKDFEKKIEESSEELIDLELPA
GLGGEKINDPVRMYLREMGTVPLLTREGEVEIARRIERGQNTVLKSLFRAPLVVQEIIGMGEEVINDTLSARDVIQIADP
MLTDEMVEEKRQEFARHTEEIARYYKKTLQCRQKLMAIPRGMKPKQYRRQLWEVARLIVRTARLCRAVKFQSPVIRHLID
RLRSAVEELRPVENDLAKTQRKLETWPAARAEAIKDLRKEQRSYGQKLQQMEEQYGASAAELRRTLEIITQAHQEAETAK
KELIEANLRLVVSIAKRYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWVRQAITRAIADQARTIRIPVHM
IETINKLIRTSRQMVQELGREPSNEELAKRLQLPVSKVRKVLRVAQEPISLETPIGEEEESHLGDFIVDQNGISPSDAVI
NLNLREQTAQVLKTLTPREEKIIKMRFGLEDGSEHTLEEVGQNFAVTRERIRQIEAKALRKLRHPSRSHRLRAFLENGTR
GV

Specific function: Also required for extracellular A-signal production. The A-signal is the first in a series of extracellular signals that are required for myxspore differentiation in M.xanthus. It is thought to act as a cell-density signal during early development to ensu

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=609, Percent_Identity=43.0213464696223, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789098, Length=226, Percent_Identity=44.6902654867257, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1789871, Length=254, Percent_Identity=30.7086614173228, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788231, Length=199, Percent_Identity=26.6331658291457, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 64753; Mature: 64621

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDDKYDGLQRLVQMGKEKGYVLYDEVSEVLPGDINGGNDLDDVLAGLDVAGIEILEEP
CCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHCC
KDFEKKIEESSEELIDLELPAGLGGEKINDPVRMYLREMGTVPLLTREGEVEIARRIERG
HHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
QNTVLKSLFRAPLVVQEIIGMGEEVINDTLSARDVIQIADPMLTDEMVEEKRQEFARHTE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIARYYKKTLQCRQKLMAIPRGMKPKQYRRQLWEVARLIVRTARLCRAVKFQSPVIRHLI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DRLRSAVEELRPVENDLAKTQRKLETWPAARAEAIKDLRKEQRSYGQKLQQMEEQYGASA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AELRRTLEIITQAHQEAETAKKELIEANLRLVVSIAKRYTNRGLQFLDLIQEGNIGLMKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
VDKFEYRRGYKFSTYATWWVRQAITRAIADQARTIRIPVHMIETINKLIRTSRQMVQELG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
REPSNEELAKRLQLPVSKVRKVLRVAQEPISLETPIGEEEESHLGDFIVDQNGISPSDAV
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCCE
INLNLREQTAQVLKTLTPREEKIIKMRFGLEDGSEHTLEEVGQNFAVTRERIRQIEAKAL
EECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
RKLRHPSRSHRLRAFLENGTRGV
HHHHCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ALDDKYDGLQRLVQMGKEKGYVLYDEVSEVLPGDINGGNDLDDVLAGLDVAGIEILEEP
CCCCHHHHHHHHHHHCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHCC
KDFEKKIEESSEELIDLELPAGLGGEKINDPVRMYLREMGTVPLLTREGEVEIARRIERG
HHHHHHHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
QNTVLKSLFRAPLVVQEIIGMGEEVINDTLSARDVIQIADPMLTDEMVEEKRQEFARHTE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIARYYKKTLQCRQKLMAIPRGMKPKQYRRQLWEVARLIVRTARLCRAVKFQSPVIRHLI
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
DRLRSAVEELRPVENDLAKTQRKLETWPAARAEAIKDLRKEQRSYGQKLQQMEEQYGASA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
AELRRTLEIITQAHQEAETAKKELIEANLRLVVSIAKRYTNRGLQFLDLIQEGNIGLMKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHH
VDKFEYRRGYKFSTYATWWVRQAITRAIADQARTIRIPVHMIETINKLIRTSRQMVQELG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
REPSNEELAKRLQLPVSKVRKVLRVAQEPISLETPIGEEEESHLGDFIVDQNGISPSDAV
CCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHCCCCCCCCCCCE
INLNLREQTAQVLKTLTPREEKIIKMRFGLEDGSEHTLEEVGQNFAVTRERIRQIEAKAL
EECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
RKLRHPSRSHRLRAFLENGTRGV
HHHHCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104614 [H]