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The map label for this gene is dnaG [H]

Identifier: 116620136

GI number: 116620136

Start: 1255837

End: 1257579

Strand: Reverse

Name: dnaG [H]

Synonym: Acid_1009

Alternate gene names: 116620136

Gene position: 1257579-1255837 (Counterclockwise)

Preceding gene: 116620138

Following gene: 116620135

Centisome position: 12.62

GC content: 61.68

Gene sequence:

>1743_bases
ATGGATTTCGTCGAGCAACTCAAGTCCTCCGTTGACATCGTGAGCGTCATCGGGGAATACGTGCGACTGCGGCGCTCGGG
GACCCAACGCTACATGGGGCTCTGCCCCTTCCACGACGAGAAGAGCCCTTCCTTCACGGTTCACGTGGTTCATCAGTTCT
ACAAGTGCTTCTCCTGCGGCGCGGGCGGCGACGTGGTGAAGTTCGTGATGGAGAAAGAGGGCATCAGTTTCTACGAGGCG
CTCAAATCGCTCTCCGAACGCTACGGCATTCCGATGCCCAAGCGCTCGCAATATGCCGATGAAGATTCGAAGCTGCGCGG
CTCGCTGCTGGTGATGCACGAGCTGGCGGCGGAGAACTTTCGGGACAATCTCAAATCGCAAGCCGGGGATAACGCGCGCG
CCTATATCGCGCAGCGCGGCCTGGCGCCCGAGACTGTGGAGCATTTCGGACTGGGATACGCGGACCGTTCGGGCCGCGCA
CTGGTGCGCCTCTTCGAACAGCGCGAGTTTTCGGTGGCGCAGATGGAACAGTCGGGGCTGGTGGGAAAGCGGCAGGACGG
CAGCCTTTACGATCGTTTCCGCAACCGCCTGATGTTTCCAATCCACAACGAGCAGGGCAAAATTATCGCCTTCGGCGGCC
GCGCGCTTTCCCCCGATGATGAACCCAAGTACCTCAACTCCCCCGAAACGGACATCTATAAGAAGAGTCACGTGCTCTAT
AACCTGCACCGCGCCAAACAGGGCATCCGCAAGGAAGATCGCGCCATCCTGGTAGAGGGATACATGGATGTCATCGGCGT
CACGGCGGCCGGCTTTCACAATGTAGTCGCCTGCTGCGGCACCGCGTTCGGGGCGCAGCAGGTGCAAGCGATCAAGCGGC
ACACGCACCGGATCGCGGTCAATTTCGATCCCGACGCGGCGGGCGCGGGCGCGTCAGAGCGCTCGCTCGATCTCCTGCTC
GCCGAGGGGATGCAAGTGCGGATCGTGGAGCTCGATGGCGGGCTTGACCCGGACGAGTACTGCAAGGAGCGCGGCACTGA
GGCTTATCAGGCTCGTATCGACGGAGCCAAGGGCTACTTCTACTGGCTTGCCGACCGCGCGCGCGCCAAGCACGATATGC
GGACCTCCGAAGGCAAGGTCGCAGTGCTCAACTTCCTGCTTCCCAAGGTGCAAAACATCGCCGATTCCCTGGAGCGCATG
ACGGTAGCCAACGACGTCGCCGGCTATATCGGCGTCGATCAGGGCATGGTGCTCGACCACTTTAAGAAGGCGGCGAGCGA
TCGCAACGAGCGCGCCATCCCCCGTCCCGCGAATCCCCTGCGGCACGACGAGCGCATGCTGCTGATCGCTCTATTGACTC
ACTCCGAGGGTCGCGCCGAGCTGATCGAACAAATGAAAGTTCTGGAAACCATCGAGACGCTGCCTTCGCGCCGCATCTTC
CAAGCGGTCTTCGCGCTCGACGAAGCAGGCGGGCGCATCGGGTTTGAGGAGTTGAATTCGCGCCTCGAAGAGGCCGACCA
AAACCTCCTGGCCCAGGCCGTGTTGAACGAGGATGGCGAAATCTCGCCCGAGGAAGTGGCGGCATCGGTGGAGAGCCTCC
GGCGCAGCGAACAGCAGTACTTGCGCCTGCAATTGAAGGCACGAATCAGCGAGAGCGAACGCGCCGGTAAATTCGACGAG
GCTGTCCGGCTTACGATGGAATTGCAGGGCATCGAAAAGGCCGCTCGCGGGAGGCGGGCATGA

Upstream 100 bases:

>100_bases
GGGGTCGCGGCGCTCGCGCGAGTCAAGTTATCGTCGGATATCGTTCCCCGGCCGGGTGTCATCGGCGTCACGCTGGGCGA
TAATAGCCAAAAGGGGCGCC

Downstream 100 bases:

>100_bases
CAGGATTGCAACCCAATGTGCGTCGTGCAGTGGTGTACAATAATCAGGATTCTTCATCCGCTGGGAGTACGGGGCTTCAG
TGGCGTTAGACGATAAATAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MDFVEQLKSSVDIVSVIGEYVRLRRSGTQRYMGLCPFHDEKSPSFTVHVVHQFYKCFSCGAGGDVVKFVMEKEGISFYEA
LKSLSERYGIPMPKRSQYADEDSKLRGSLLVMHELAAENFRDNLKSQAGDNARAYIAQRGLAPETVEHFGLGYADRSGRA
LVRLFEQREFSVAQMEQSGLVGKRQDGSLYDRFRNRLMFPIHNEQGKIIAFGGRALSPDDEPKYLNSPETDIYKKSHVLY
NLHRAKQGIRKEDRAILVEGYMDVIGVTAAGFHNVVACCGTAFGAQQVQAIKRHTHRIAVNFDPDAAGAGASERSLDLLL
AEGMQVRIVELDGGLDPDEYCKERGTEAYQARIDGAKGYFYWLADRARAKHDMRTSEGKVAVLNFLLPKVQNIADSLERM
TVANDVAGYIGVDQGMVLDHFKKAASDRNERAIPRPANPLRHDERMLLIALLTHSEGRAELIEQMKVLETIETLPSRRIF
QAVFALDEAGGRIGFEELNSRLEEADQNLLAQAVLNEDGEISPEEVAASVESLRRSEQQYLRLQLKARISESERAGKFDE
AVRLTMELQGIEKAARGRRA

Sequences:

>Translated_580_residues
MDFVEQLKSSVDIVSVIGEYVRLRRSGTQRYMGLCPFHDEKSPSFTVHVVHQFYKCFSCGAGGDVVKFVMEKEGISFYEA
LKSLSERYGIPMPKRSQYADEDSKLRGSLLVMHELAAENFRDNLKSQAGDNARAYIAQRGLAPETVEHFGLGYADRSGRA
LVRLFEQREFSVAQMEQSGLVGKRQDGSLYDRFRNRLMFPIHNEQGKIIAFGGRALSPDDEPKYLNSPETDIYKKSHVLY
NLHRAKQGIRKEDRAILVEGYMDVIGVTAAGFHNVVACCGTAFGAQQVQAIKRHTHRIAVNFDPDAAGAGASERSLDLLL
AEGMQVRIVELDGGLDPDEYCKERGTEAYQARIDGAKGYFYWLADRARAKHDMRTSEGKVAVLNFLLPKVQNIADSLERM
TVANDVAGYIGVDQGMVLDHFKKAASDRNERAIPRPANPLRHDERMLLIALLTHSEGRAELIEQMKVLETIETLPSRRIF
QAVFALDEAGGRIGFEELNSRLEEADQNLLAQAVLNEDGEISPEEVAASVESLRRSEQQYLRLQLKARISESERAGKFDE
AVRLTMELQGIEKAARGRRA
>Mature_580_residues
MDFVEQLKSSVDIVSVIGEYVRLRRSGTQRYMGLCPFHDEKSPSFTVHVVHQFYKCFSCGAGGDVVKFVMEKEGISFYEA
LKSLSERYGIPMPKRSQYADEDSKLRGSLLVMHELAAENFRDNLKSQAGDNARAYIAQRGLAPETVEHFGLGYADRSGRA
LVRLFEQREFSVAQMEQSGLVGKRQDGSLYDRFRNRLMFPIHNEQGKIIAFGGRALSPDDEPKYLNSPETDIYKKSHVLY
NLHRAKQGIRKEDRAILVEGYMDVIGVTAAGFHNVVACCGTAFGAQQVQAIKRHTHRIAVNFDPDAAGAGASERSLDLLL
AEGMQVRIVELDGGLDPDEYCKERGTEAYQARIDGAKGYFYWLADRARAKHDMRTSEGKVAVLNFLLPKVQNIADSLERM
TVANDVAGYIGVDQGMVLDHFKKAASDRNERAIPRPANPLRHDERMLLIALLTHSEGRAELIEQMKVLETIETLPSRRIF
QAVFALDEAGGRIGFEELNSRLEEADQNLLAQAVLNEDGEISPEEVAASVESLRRSEQQYLRLQLKARISESERAGKFDE
AVRLTMELQGIEKAARGRRA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=486, Percent_Identity=30.8641975308642, Blast_Score=261, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65013; Mature: 65013

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFVEQLKSSVDIVSVIGEYVRLRRSGTQRYMGLCPFHDEKSPSFTVHVVHQFYKCFSCG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEHHHHHHHHHHHCCC
AGGDVVKFVMEKEGISFYEALKSLSERYGIPMPKRSQYADEDSKLRGSLLVMHELAAENF
CCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RDNLKSQAGDNARAYIAQRGLAPETVEHFGLGYADRSGRALVRLFEQREFSVAQMEQSGL
HHHHHHHCCCCHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
VGKRQDGSLYDRFRNRLMFPIHNEQGKIIAFGGRALSPDDEPKYLNSPETDIYKKSHVLY
CCCCCCCHHHHHHHCCEECEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
NLHRAKQGIRKEDRAILVEGYMDVIGVTAAGFHNVVACCGTAFGAQQVQAIKRHTHRIAV
HHHHHHHCCCHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEE
NFDPDAAGAGASERSLDLLLAEGMQVRIVELDGGLDPDEYCKERGTEAYQARIDGAKGYF
EECCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCHHHHHHCCCCCEEE
YWLADRARAKHDMRTSEGKVAVLNFLLPKVQNIADSLERMTVANDVAGYIGVDQGMVLDH
EEECHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FKKAASDRNERAIPRPANPLRHDERMLLIALLTHSEGRAELIEQMKVLETIETLPSRRIF
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHH
QAVFALDEAGGRIGFEELNSRLEEADQNLLAQAVLNEDGEISPEEVAASVESLRRSEQQY
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LRLQLKARISESERAGKFDEAVRLTMELQGIEKAARGRRA
HHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]