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The map label for this gene is rsmE [H]

Identifier: 116620083

GI number: 116620083

Start: 1193934

End: 1194647

Strand: Reverse

Name: rsmE [H]

Synonym: Acid_0955

Alternate gene names: 116620083

Gene position: 1194647-1193934 (Counterclockwise)

Preceding gene: 116620088

Following gene: 116620082

Centisome position: 11.99

GC content: 66.81

Gene sequence:

>714_bases
GTGGCCCGACGTAGGTTTTTCGTAGACGCAATCCGCAATGGTGCGGCCGAACTGCGCGGCGACGAGGCGCGGCACTTAAC
GCGCGTGCTTCGCGTGGAGTCCGGGCAGCGCTTCGAGATCTCGGACAATCAGCACGCGTACCTCGCGGAGATCGCGGAGG
CAAAAGGCGAGCGCGTGGTCTTCCGCGTGCTAGAAGCAATCGCACCAGTTCCGATGCCGGTACGAATTACCCTGTGCGCC
GCGATCGTCAAGTTCGACCGCTTCGAGTGGATGATCGAGAAGGCGACGGAACTCGGGGTGGAGCGGATCCTGCCCGTGGA
GGCCACGCGCACCGAGCGCGGGTTATTCGAGGCGTCGCGCAAGCGCAGCGAGCGGTGGACGCGGATCGCGCGCGAGAGCA
GCCAGCAATCACGGCGCGTGCAGATCCCCGAGATCCTGCCGGCCGTGCGCCTGGAGGAGTGTTTGAAGGAGACGGCTGAG
CACCGCTACTTCCTGGATGAAGGGGCGGCGCCGCCCCTGGTGCGCGAGATTCCGGCGGAGCGTTCGGACACCGTGGCGCT
GTTGATCGGCCCGGAAGGCGGCTGGACGGAGGCCGAACGCGGGCTGGCGGTAAGCGCCGGGTGGTGTCCGGTTTCACTGG
GACCGCTGGTGCTTCGTGCGGAAACAGCCGCCGCAGCCGCAATCGCGGTCGTCATCAACACGTGGATGGCGTGA

Upstream 100 bases:

>100_bases
CTACCAGCACCTGGTTCGGATGTTTCTCCACGACTAACCCGCGCAGTAAGGCGATCATCAGTAATGATTGTAGCCACTTG
GAGTCGCTACAATAAGTGCC

Downstream 100 bases:

>100_bases
GAAAATAAAAAACGACAGTGGCCAATTATTCCCTGGATCTCAGTCTCATCGTCATCTTTACCCTGGTACAGTGGCGAATC
ACTGTGATCATCCTGAGGGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MARRRFFVDAIRNGAAELRGDEARHLTRVLRVESGQRFEISDNQHAYLAEIAEAKGERVVFRVLEAIAPVPMPVRITLCA
AIVKFDRFEWMIEKATELGVERILPVEATRTERGLFEASRKRSERWTRIARESSQQSRRVQIPEILPAVRLEECLKETAE
HRYFLDEGAAPPLVREIPAERSDTVALLIGPEGGWTEAERGLAVSAGWCPVSLGPLVLRAETAAAAAIAVVINTWMA

Sequences:

>Translated_237_residues
MARRRFFVDAIRNGAAELRGDEARHLTRVLRVESGQRFEISDNQHAYLAEIAEAKGERVVFRVLEAIAPVPMPVRITLCA
AIVKFDRFEWMIEKATELGVERILPVEATRTERGLFEASRKRSERWTRIARESSQQSRRVQIPEILPAVRLEECLKETAE
HRYFLDEGAAPPLVREIPAERSDTVALLIGPEGGWTEAERGLAVSAGWCPVSLGPLVLRAETAAAAAIAVVINTWMA
>Mature_236_residues
ARRRFFVDAIRNGAAELRGDEARHLTRVLRVESGQRFEISDNQHAYLAEIAEAKGERVVFRVLEAIAPVPMPVRITLCAA
IVKFDRFEWMIEKATELGVERILPVEATRTERGLFEASRKRSERWTRIARESSQQSRRVQIPEILPAVRLEECLKETAEH
RYFLDEGAAPPLVREIPAERSDTVALLIGPEGGWTEAERGLAVSAGWCPVSLGPLVLRAETAAAAAIAVVINTWMA

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=216, Percent_Identity=34.2592592592593, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26537; Mature: 26406

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRRFFVDAIRNGAAELRGDEARHLTRVLRVESGQRFEISDNQHAYLAEIAEAKGERVV
CCCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCHHH
FRVLEAIAPVPMPVRITLCAAIVKFDRFEWMIEKATELGVERILPVEATRTERGLFEASR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KRSERWTRIARESSQQSRRVQIPEILPAVRLEECLKETAEHRYFLDEGAAPPLVREIPAE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEECCCCCCCHHHHHCCCC
RSDTVALLIGPEGGWTEAERGLAVSAGWCPVSLGPLVLRAETAAAAAIAVVINTWMA
CCCEEEEEECCCCCCCHHHCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARRRFFVDAIRNGAAELRGDEARHLTRVLRVESGQRFEISDNQHAYLAEIAEAKGERVV
CCHHHHHHHHHCCHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCCHHH
FRVLEAIAPVPMPVRITLCAAIVKFDRFEWMIEKATELGVERILPVEATRTERGLFEASR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
KRSERWTRIARESSQQSRRVQIPEILPAVRLEECLKETAEHRYFLDEGAAPPLVREIPAE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCEECCCCCCCHHHHHCCCC
RSDTVALLIGPEGGWTEAERGLAVSAGWCPVSLGPLVLRAETAAAAAIAVVINTWMA
CCCEEEEEECCCCCCCHHHCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]