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The map label for this gene is tolB [H]

Identifier: 116619729

GI number: 116619729

Start: 731870

End: 732877

Strand: Direct

Name: tolB [H]

Synonym: Acid_0594

Alternate gene names: 116619729

Gene position: 731870-732877 (Clockwise)

Preceding gene: 116619725

Following gene: 116619736

Centisome position: 7.34

GC content: 58.63

Gene sequence:

>1008_bases
ATGAAGAAGACTGTATGCATGTTCGCGGCGCTTGCCGCAGTCGCCGTGGCGCAATCCCAACACCTGAGCGATTACAAACA
ACTGACTCACGGTGGACAAAATGCCGAGGCGTATTGGTCTCCCGACGGCAAACGCCTGATCTTCCAGACCACACGTCCGC
CCTACGATTGCGACCAGATCTTCCTGATGAACGCCGATGGCAGCGACCAGCACCTGGCCTCCACCGGCAAAGGCCGCACC
ACCTGCGGCTACTTCCTGGCCGATGGCAAGCACATCGTCTACGGCTCCACTCACCTGGCCGGTGATGCCTGCCCCGTTTC
GCCCGACCGCAGCAAGGGTTACGTGTGGGGCGTCTTCGCGGGTTACGACATCTTCGAAGCGAACGACATCGGCGAGATTG
AAAAGCGCCTCACCAACACGCCCGGCTACGACGCCGAAGCCACCGTAAACTGGAAGACCAACAACATCGTATACACCAGC
GTCGCCAGCGGCGATCTGGACCTCTGGACTATGAAACCCGATGGCAGCGCCAAGAAACAGATCACCAAAAACCCGGGCTA
CGATGGCGGCGCCGTGTTCTCCCGCGACGGCAAGCAACTGGTGTGGCGAGCCGGCTATCCCAAGAACGATGCCGACATGA
AGCGATACAAAGAACTCCTCGCCGAGAATCTTACCGCTCCGATGAAGATGGAACTGATGGTCTCCAACGCCGATGGCACT
TCCGCCCGGCAGATCACCAATTTCGGCTGCGCCAGCTTCGCCCCGACCTTCGCGCCGGATGGAAAGAAGATCCTCTTCGC
CTCCAACAAGGCCGAATGCGACAGCCGCAAGTTTGAACTCTATCTGATCAACGTCGATGGCACGGGCCTCGAGCAAGTCA
CCAATTTCGGCGGCTTCACCAGCTTCCCGGAATTCTCCCCGGACGGCAAAACCCTCGTTTTCTGCACCGACAAAGACGCC
AAAGAGCGGTACGAATTCAACATCTTCACCGCCAAATGGTCGAACTAG

Upstream 100 bases:

>100_bases
ACAAACGCCGGCGGCGCGGCGGCGATGAGTGAAAAGCCCAGGGAGAAGGCCAGCAAAAGTGACAAACGGCGCACGCTCCA
CAGTATACTGAAACACCGGT

Downstream 100 bases:

>100_bases
CGAGTGATCCAGCGGATCGCTCCTGACGCTCGCTGCGCTTACTGCGCCGTGGCATCGAGTACTTCACGGACTTTTTGTAG
CAGGGCGTCGGGGGTAAAGG

Product: WD40 domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKKTVCMFAALAAVAVAQSQHLSDYKQLTHGGQNAEAYWSPDGKRLIFQTTRPPYDCDQIFLMNADGSDQHLASTGKGRT
TCGYFLADGKHIVYGSTHLAGDACPVSPDRSKGYVWGVFAGYDIFEANDIGEIEKRLTNTPGYDAEATVNWKTNNIVYTS
VASGDLDLWTMKPDGSAKKQITKNPGYDGGAVFSRDGKQLVWRAGYPKNDADMKRYKELLAENLTAPMKMELMVSNADGT
SARQITNFGCASFAPTFAPDGKKILFASNKAECDSRKFELYLINVDGTGLEQVTNFGGFTSFPEFSPDGKTLVFCTDKDA
KERYEFNIFTAKWSN

Sequences:

>Translated_335_residues
MKKTVCMFAALAAVAVAQSQHLSDYKQLTHGGQNAEAYWSPDGKRLIFQTTRPPYDCDQIFLMNADGSDQHLASTGKGRT
TCGYFLADGKHIVYGSTHLAGDACPVSPDRSKGYVWGVFAGYDIFEANDIGEIEKRLTNTPGYDAEATVNWKTNNIVYTS
VASGDLDLWTMKPDGSAKKQITKNPGYDGGAVFSRDGKQLVWRAGYPKNDADMKRYKELLAENLTAPMKMELMVSNADGT
SARQITNFGCASFAPTFAPDGKKILFASNKAECDSRKFELYLINVDGTGLEQVTNFGGFTSFPEFSPDGKTLVFCTDKDA
KERYEFNIFTAKWSN
>Mature_335_residues
MKKTVCMFAALAAVAVAQSQHLSDYKQLTHGGQNAEAYWSPDGKRLIFQTTRPPYDCDQIFLMNADGSDQHLASTGKGRT
TCGYFLADGKHIVYGSTHLAGDACPVSPDRSKGYVWGVFAGYDIFEANDIGEIEKRLTNTPGYDAEATVNWKTNNIVYTS
VASGDLDLWTMKPDGSAKKQITKNPGYDGGAVFSRDGKQLVWRAGYPKNDADMKRYKELLAENLTAPMKMELMVSNADGT
SARQITNFGCASFAPTFAPDGKKILFASNKAECDSRKFELYLINVDGTGLEQVTNFGGFTSFPEFSPDGKTLVFCTDKDA
KERYEFNIFTAKWSN

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Caenorhabditis elegans, GI32564215, Length=343, Percent_Identity=42.5655976676385, Blast_Score=241, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI32564217, Length=343, Percent_Identity=42.5655976676385, Blast_Score=241, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 36880; Mature: 36880

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVCMFAALAAVAVAQSQHLSDYKQLTHGGQNAEAYWSPDGKRLIFQTTRPPYDCDQI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCCCCCCEE
FLMNADGSDQHLASTGKGRTTCGYFLADGKHIVYGSTHLAGDACPVSPDRSKGYVWGVFA
EEECCCCCCHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEE
GYDIFEANDIGEIEKRLTNTPGYDAEATVNWKTNNIVYTSVASGDLDLWTMKPDGSAKKQ
CCEEEECCCHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCEEEEEECCCCCHHHH
ITKNPGYDGGAVFSRDGKQLVWRAGYPKNDADMKRYKELLAENLTAPMKMELMVSNADGT
HCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
SARQITNFGCASFAPTFAPDGKKILFASNKAECDSRKFELYLINVDGTGLEQVTNFGGFT
CHHHHHCCCCHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHCCCCCC
SFPEFSPDGKTLVFCTDKDAKERYEFNIFTAKWSN
CCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MKKTVCMFAALAAVAVAQSQHLSDYKQLTHGGQNAEAYWSPDGKRLIFQTTRPPYDCDQI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCCCCCCEE
FLMNADGSDQHLASTGKGRTTCGYFLADGKHIVYGSTHLAGDACPVSPDRSKGYVWGVFA
EEECCCCCCHHHHHCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEE
GYDIFEANDIGEIEKRLTNTPGYDAEATVNWKTNNIVYTSVASGDLDLWTMKPDGSAKKQ
CCEEEECCCHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCEEEEEECCCCCHHHH
ITKNPGYDGGAVFSRDGKQLVWRAGYPKNDADMKRYKELLAENLTAPMKMELMVSNADGT
HCCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCC
SARQITNFGCASFAPTFAPDGKKILFASNKAECDSRKFELYLINVDGTGLEQVTNFGGFT
CHHHHHCCCCHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEEEECCCCHHHHHCCCCCC
SFPEFSPDGKTLVFCTDKDAKERYEFNIFTAKWSN
CCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]