Click here to switch to the map view.

The map label for this gene is adhE [H]

Identifier: 116619523

GI number: 116619523

Start: 482313

End: 484019

Strand: Direct

Name: adhE [H]

Synonym: Acid_0382

Alternate gene names: 116619523

Gene position: 482313-484019 (Clockwise)

Preceding gene: 116619522

Following gene: 116619524

Centisome position: 4.84

GC content: 62.04

Gene sequence:

>1707_bases
ATGCTCGAGGATAAGGACCTCCTGTCCATCCAGGAGGTACGCAGCAAAGTCGAGAAGGCGTTCGCCGCCTGGCAGAAATA
CAAGTCGTTCTCACAAGAGCAGGTGGACGCAATTGTGGAACGCATGGCTGCCGCGGCGCGAGCGAACGCTAAGCGCCTGG
CCGACCTGGCTGTGGAAGAGACTGGCTACGGCAACGCCAAAGACAAATACATCAAGAACCTGTTGACCAGCGACTGGCTA
CCGCGCCGCATGCGCGGAATGCGTACGGTCGGCATCGTGCGCGAGCTGCCGGAAGAGAAGGTGGTGGAGATCGGCGTTTC
GATGGGTGTGGTAGCGGCGGTGCTGCCCACGACCAACCCGACTTCCACGGCGATTTACAAGATCCTGATTTCGATGAAAT
CGGGCAACTCGATTGTGCTCAGCCCGCATCCGCGTGCGTTCAAGTGCACGTGTGAGACGGCCTCGGTCCTATACCAGGCG
GCGCTCGAGGCTGGGGCTCCGGAAGACGTCATCCAGTGCATCGATCATGCCACCATGGAAGGCATTAGCGCGCTGATGCA
TCATGAACGCATCGCCGTGATCCTGGCGACGGGCGGACACGCGATGGTGAAATCGGCGTACTCGAGTGGGAAGCCGGCGT
ACGGAGTGGGACCGGGCAACGTGCCCGTGCTGGTGGACACTTCGGCCGACCTCGAAGACGCGATCCCCAAGATCGTGGCC
GGCAAGAGTTTCGATTACGGCACGGTTTGTTCCAGCGAGCAGGCGATTGTGGCGGAGTACACGATGCGCGATCGCATCCT
GGCGCTGCTCAAACAGAACAAGGCGTATATCTGCAATGATGCGCAGAAGGACGCGCTGGGCAAGCTGCTGCTCACGCCGA
ACTGGACGGTCAATCCGAACTGCGTGGGGCAGGCTCCGACCAAGATTGCAAAGATGGCAGGGTTTGAGGTGCCGCCGGAG
ACCTCCATCATCTGCTGCGAGATTGAGGGCGTCGGCAAGAAATATCCGTTGTCGGCGGAGAAGCTCTCGCCGGTACTGTC
GCTGATTTTCGTGCCGGATTTCAACGCGGCGCTGGATACCTGTTTCGGGCTGCTGAAATTTGGCGGAGCGGGGCATACGG
CGGCGATTTACGGCAAGAACGATACGCGCATCCGGGAATATGCGATGCGCATGCCGGCGTACCGCGTGCTGGTGAATACG
CCCGCGCCGCAAGGATCCACCGGCATCACGACGAATGTTTTCCCGGCGATGACGCTGGGATGCGGCGCAGTGGCGGGGAA
CAGCACGTCGGACAATGTGGGGCCGCAGCACCTGATGAATATCAAGCGGTTGGCCTACACGGTACGCAAGCCGGAAGAGG
CGTTCGAGATGCCGCTGGACTACGACAAGGCGCCTTACAGCGCGGCCGTAGCGGCTGGCGTTGCGGGCGGCATCGATCGG
TCTACGGTGGTATCCGCAGTGGAGCGCTATCTGGCGTCGCGCGGCATCGCGGTTGCTCCGCAGGCAACGGTTCCGGCGGC
ATCGGCCAGCAGTGGCTCGTGCGGCTGTTCGATACCGGCGCCGGCCAAGGCTCCGGTGACGCAATCCACGGTGGCTAGCG
TTGCCGCCGAAGTGGTGGATCGCTTTTTGGCCGGGCGCGGAACGGCTCCGCAGAACCCATCGACGCCGGCTTCGAGCAGT
TCGCAGAATACCGGCGGCTACAGTTGA

Upstream 100 bases:

>100_bases
CCGCCGTGTCGGGGAACTGGTCAGCGTCCACGTGATCCCGCGGCCGCACGAAGAGGTCGAGAAAATTCTCCCGGCCGGCG
GCAACAAGAAGTAAGGACCG

Downstream 100 bases:

>100_bases
CTGACCGCTCCTGGCGCAAAAACGTCTCCAGCCCCCGCAACTCCGCCACTCAAGACGGTGGACTTTGTGTGCGAGGCCGA
CGTGCGCCAGGCCATTCAAG

Product: aldehyde dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase; ADH; Acetaldehyde dehydrogenase [acetylating]; ACDH; Pyruvate-formate-lyase deactivase; PFL deactivase [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MLEDKDLLSIQEVRSKVEKAFAAWQKYKSFSQEQVDAIVERMAAAARANAKRLADLAVEETGYGNAKDKYIKNLLTSDWL
PRRMRGMRTVGIVRELPEEKVVEIGVSMGVVAAVLPTTNPTSTAIYKILISMKSGNSIVLSPHPRAFKCTCETASVLYQA
ALEAGAPEDVIQCIDHATMEGISALMHHERIAVILATGGHAMVKSAYSSGKPAYGVGPGNVPVLVDTSADLEDAIPKIVA
GKSFDYGTVCSSEQAIVAEYTMRDRILALLKQNKAYICNDAQKDALGKLLLTPNWTVNPNCVGQAPTKIAKMAGFEVPPE
TSIICCEIEGVGKKYPLSAEKLSPVLSLIFVPDFNAALDTCFGLLKFGGAGHTAAIYGKNDTRIREYAMRMPAYRVLVNT
PAPQGSTGITTNVFPAMTLGCGAVAGNSTSDNVGPQHLMNIKRLAYTVRKPEEAFEMPLDYDKAPYSAAVAAGVAGGIDR
STVVSAVERYLASRGIAVAPQATVPAASASSGSCGCSIPAPAKAPVTQSTVASVAAEVVDRFLAGRGTAPQNPSTPASSS
SQNTGGYS

Sequences:

>Translated_568_residues
MLEDKDLLSIQEVRSKVEKAFAAWQKYKSFSQEQVDAIVERMAAAARANAKRLADLAVEETGYGNAKDKYIKNLLTSDWL
PRRMRGMRTVGIVRELPEEKVVEIGVSMGVVAAVLPTTNPTSTAIYKILISMKSGNSIVLSPHPRAFKCTCETASVLYQA
ALEAGAPEDVIQCIDHATMEGISALMHHERIAVILATGGHAMVKSAYSSGKPAYGVGPGNVPVLVDTSADLEDAIPKIVA
GKSFDYGTVCSSEQAIVAEYTMRDRILALLKQNKAYICNDAQKDALGKLLLTPNWTVNPNCVGQAPTKIAKMAGFEVPPE
TSIICCEIEGVGKKYPLSAEKLSPVLSLIFVPDFNAALDTCFGLLKFGGAGHTAAIYGKNDTRIREYAMRMPAYRVLVNT
PAPQGSTGITTNVFPAMTLGCGAVAGNSTSDNVGPQHLMNIKRLAYTVRKPEEAFEMPLDYDKAPYSAAVAAGVAGGIDR
STVVSAVERYLASRGIAVAPQATVPAASASSGSCGCSIPAPAKAPVTQSTVASVAAEVVDRFLAGRGTAPQNPSTPASSS
SQNTGGYS
>Mature_568_residues
MLEDKDLLSIQEVRSKVEKAFAAWQKYKSFSQEQVDAIVERMAAAARANAKRLADLAVEETGYGNAKDKYIKNLLTSDWL
PRRMRGMRTVGIVRELPEEKVVEIGVSMGVVAAVLPTTNPTSTAIYKILISMKSGNSIVLSPHPRAFKCTCETASVLYQA
ALEAGAPEDVIQCIDHATMEGISALMHHERIAVILATGGHAMVKSAYSSGKPAYGVGPGNVPVLVDTSADLEDAIPKIVA
GKSFDYGTVCSSEQAIVAEYTMRDRILALLKQNKAYICNDAQKDALGKLLLTPNWTVNPNCVGQAPTKIAKMAGFEVPPE
TSIICCEIEGVGKKYPLSAEKLSPVLSLIFVPDFNAALDTCFGLLKFGGAGHTAAIYGKNDTRIREYAMRMPAYRVLVNT
PAPQGSTGITTNVFPAMTLGCGAVAGNSTSDNVGPQHLMNIKRLAYTVRKPEEAFEMPLDYDKAPYSAAVAAGVAGGIDR
STVVSAVERYLASRGIAVAPQATVPAASASSGSCGCSIPAPAKAPVTQSTVASVAAEVVDRFLAGRGTAPQNPSTPASSS
SQNTGGYS

Specific function: This enzyme has three activities:ADH, ACDH, and PFL- deactivase. In aerobic conditions it acts as a hydrogen peroxide scavenger. The PFL deactivase activity catalyzes the quenching of the pyruvate-formate-lyase catalyst in an iron, NAD, and CoA dependent

COG id: COG1012

COG function: function code C; NAD-dependent aldehyde dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the iron- containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=498, Percent_Identity=38.9558232931727, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1788797, Length=375, Percent_Identity=29.8666666666667, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1787558, Length=252, Percent_Identity=25.7936507936508, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR012079 [H]

Pfam domain/function: PF00171 Aldedh; PF00465 Fe-ADH [H]

EC number: =1.1.1.1; =1.2.1.10 [H]

Molecular weight: Translated: 59988; Mature: 59988

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEDKDLLSIQEVRSKVEKAFAAWQKYKSFSQEQVDAIVERMAAAARANAKRLADLAVEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGYGNAKDKYIKNLLTSDWLPRRMRGMRTVGIVRELPEEKVVEIGVSMGVVAAVLPTTNP
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCC
TSTAIYKILISMKSGNSIVLSPHPRAFKCTCETASVLYQAALEAGAPEDVIQCIDHATME
HHHHHHHHHEEECCCCEEEECCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GISALMHHERIAVILATGGHAMVKSAYSSGKPAYGVGPGNVPVLVDTSADLEDAIPKIVA
HHHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHC
GKSFDYGTVCSSEQAIVAEYTMRDRILALLKQNKAYICNDAQKDALGKLLLTPNWTVNPN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHCEEEECCCCCCCCC
CVGQAPTKIAKMAGFEVPPETSIICCEIEGVGKKYPLSAEKLSPVLSLIFVPDFNAALDT
CCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHEEECCCCHHHHHH
CFGLLKFGGAGHTAAIYGKNDTRIREYAMRMPAYRVLVNTPAPQGSTGITTNVFPAMTLG
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHC
CGAVAGNSTSDNVGPQHLMNIKRLAYTVRKPEEAFEMPLDYDKAPYSAAVAAGVAGGIDR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCH
STVVSAVERYLASRGIAVAPQATVPAASASSGSCGCSIPAPAKAPVTQSTVASVAAEVVD
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RFLAGRGTAPQNPSTPASSSSQNTGGYS
HHHHCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLEDKDLLSIQEVRSKVEKAFAAWQKYKSFSQEQVDAIVERMAAAARANAKRLADLAVEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGYGNAKDKYIKNLLTSDWLPRRMRGMRTVGIVRELPEEKVVEIGVSMGVVAAVLPTTNP
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCC
TSTAIYKILISMKSGNSIVLSPHPRAFKCTCETASVLYQAALEAGAPEDVIQCIDHATME
HHHHHHHHHEEECCCCEEEECCCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GISALMHHERIAVILATGGHAMVKSAYSSGKPAYGVGPGNVPVLVDTSADLEDAIPKIVA
HHHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHC
GKSFDYGTVCSSEQAIVAEYTMRDRILALLKQNKAYICNDAQKDALGKLLLTPNWTVNPN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHCEEEECCCCCCCCC
CVGQAPTKIAKMAGFEVPPETSIICCEIEGVGKKYPLSAEKLSPVLSLIFVPDFNAALDT
CCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHEEECCCCHHHHHH
CFGLLKFGGAGHTAAIYGKNDTRIREYAMRMPAYRVLVNTPAPQGSTGITTNVFPAMTLG
HHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHC
CGAVAGNSTSDNVGPQHLMNIKRLAYTVRKPEEAFEMPLDYDKAPYSAAVAAGVAGGIDR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCH
STVVSAVERYLASRGIAVAPQATVPAASASSGSCGCSIPAPAKAPVTQSTVASVAAEVVD
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RFLAGRGTAPQNPSTPASSSSQNTGGYS
HHHHCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]