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The map label for this gene is nasD [H]

Identifier: 116619327

GI number: 116619327

Start: 235825

End: 238230

Strand: Direct

Name: nasD [H]

Synonym: Acid_0184

Alternate gene names: 116619327

Gene position: 235825-238230 (Clockwise)

Preceding gene: 116619326

Following gene: 116619328

Centisome position: 2.37

GC content: 62.93

Gene sequence:

>2406_bases
ATGAAAAAACTCGTAGTCGTCGGCAACGGCATGGCCGGAGTAGCGTGCGTAGAGCAGATTCTCAAGTACCGGCACGAATT
TCAAATTACCATCATCGGCGACGAAACCCACGTCAACTACAACCGCATCCTCCTCTCCTCGGTGCTCGCCGGCGAGAAAT
CCGCGGACGATATCGTGCTCAACGATATCGAGTGGTACCGCGCGAATCACATCACGCCGCGCCTTGGCCTGCGCGTTGCC
GCCATCGAAGCCGGCGAACGCATCGTGCGCGGCACGGATGGCTCCGCCATCCCCTTCGACAAACTGATTCTGGCCACCGG
CAGCACCGCCTTCGTGCCTCCCGTCCCGGGCACCGACAAGCAGAACGTCCACGTCTTCCGCACCCTTGACGATACCCGCG
CCCTGCTCGAAAAATCGCGGCCTGGCCGCAAGGCCGTAGTCATCGGCGGCGGATTGCTCGGCCTGGAAGCCGCCCGCGGA
TTGCAGGTCCAGGGCTGCGACGTGACCGTCGTCCACCTCGCGCCCACCTTAATGGAGAGGCAGCTCGATGCCGGCGGCGG
CGCCTATCTCGCCCGCAAGATGGAGAACCTGGGGGTCAAGGTTCTACTTGGCCGTCAGACCCAGGCCATGCTCGGCAACG
GCCGCGTCGAAGGGCTGCGCTTCAAAGGCGGTGAGGAAGTGGAAGCCGAACTGGTCGTGGTTGCCGCCGGCATCCGCCCC
AACATCGAACTCGGCCGCACCGCCGGCCTCGAAGTCCGCCGCGGCATCGTGGTCAACGACTACATGGAAACGTCGAACCC
CGATATCTTCGCGGTCGGCGAATGCACCGAGCATCGCGGACAAATCTTCGGCCTCGTCGCTCCCCTCATGGAACAGGGCA
AAGTGCTCGCCGCCACCATCACAGGAAATCGCGGCCCCGTCTTCCTCGGCGCCAGCACCGCCGCCAAATTGAAAATCATG
GGCGTCGAGGTCTTCTCCGCCGGCAGCATCGACGATACCGAGCGCGGCGTCGAAACCGTTCGCTACGAAGACCCCGCGCT
CGGCATCTACAAAAAGCTGCTGATCAAAGACAACCGGCTGCACGGCGTCATCCTGGTCGGCGACGCTTCCGACGATCATC
GCTATAAAGCCTGGCTCACCGAGGCCACTGACCTCACACCGCACCGCCGCCACCTGCTCTTCCCTCCGCCCGCGCAGGAT
GCCGGCCTCGACGTCGCCCAGATACCCGATCGCGAAACCATCTGCGGCTGCATGGGCGTCACGAAGGGCGAACTCATAAC
CGCCATCCACCAGCACGGCATCGCCACTATGACGGAGCTCAAAGATCGCACGCGCGCTTCCACCGGCTGCGGCAGCTGCG
GCAACCTCTGCGCCCGCCTACTCAAGGCCGTCGCACCCGAGTTTGAAGAGGAGAAGCAGAAGGTCCTCTGCGCCTGCGTG
CCTTTCCCGCAGGAGAAACTGCGCGAGATCGTGCGCTCCCAGCAACTCCGCTCCGTGCAGGACGTGCTCGATGTCTATGG
CAACGGCCGCGGCTGCGAAATCTGCAAGCCCGCGCTCAGCTACATGGTCGATATGGTCTGGTGCGGCGATCACGAAGAAG
ATCGCTCCGCGCGCTTCATCAACGATCGCGTGCACGCCAACATTCAGAAGGATGGAACCTTCTCGGTTGTCCCCCGCATG
CGAGGCGGCGTCACCACGCCCGCCGAACTGCGCCGCATCGCCGATGTCGCCGAGAAGTACCATGTGCCCCTTGTGAAGGT
CACCGGTAGCCAGCGTATCGACCTCCTCGGTGTGCACAAAAGCGATCTTCCCGCCATCTGGTCGGAACTCGGCATGCCGT
CCGGCCAGGCCTACACCAAGGGTGTCCGCATGGTGAAGACCTGCGTGGGCAGCCAGTTCTGCCGCTTCGGCACCCAGGAC
GCGATCGCCGCCGGAGTCGACCTCGAAACGCGGCTCGAGAATCTCTACTCGCCCCACAAGTTCAAGATGGCCGTGGTCGG
CTGTCCGCGTAATTGCGCCGAGGCCACCGTTAAGGACGTCGGCCTGGTCGGTAACGAAGGCAGCTGGCAAGTGGTGGTCG
GCGGAGCCGCCGGTAAGAGCGTCCGCAAAGCCGACCTGCTGGTTACCGTCGAAACCACCGACGCAGCGCTCGAAGCCGCC
GAAGTCTTCTTCCAGTACTACCGCGAGAACGCTAATTACCTGGAGCGCAGTTACGATTTCGTGGAGCGTCTCGGCATCGA
AAAGGTGCGCAAGGAAACCGTGTACTCGCTTCCCGAAGTCCGCCGCGCATTGCTGGACCGCTTGCACAAATCCAAAGACA
AATCCTCGGACGCCTGGCTTGAGCGCATCAACCCGCGTCCCACGCAGTTCGTGAAAATTCAACCCATGGAGACCGTATCC
CTATGA

Upstream 100 bases:

>100_bases
GCAACGCGAACGAAGTCACCCAAAGCGCGTTCGACCCGGTATCCCGCGAGCCCAACTATAAGCAATGCGCGGTGCGAGTC
GAACGAACTGGAGCAGCCGT

Downstream 100 bases:

>100_bases
ACTGGATTCGCATCACCGCCGCCGAAAACATCCCGCCGCGCGAAGGCCGGACCGTACAGTTAGGGGACCTGTCCCTCGCC
ATCTTCAACACGGGCGAGCG

Product: assimilatory nitrite reductase (NAD(P)H) large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKKLVVVGNGMAGVACVEQILKYRHEFQITIIGDETHVNYNRILLSSVLAGEKSADDIVLNDIEWYRANHITPRLGLRVA
AIEAGERIVRGTDGSAIPFDKLILATGSTAFVPPVPGTDKQNVHVFRTLDDTRALLEKSRPGRKAVVIGGGLLGLEAARG
LQVQGCDVTVVHLAPTLMERQLDAGGGAYLARKMENLGVKVLLGRQTQAMLGNGRVEGLRFKGGEEVEAELVVVAAGIRP
NIELGRTAGLEVRRGIVVNDYMETSNPDIFAVGECTEHRGQIFGLVAPLMEQGKVLAATITGNRGPVFLGASTAAKLKIM
GVEVFSAGSIDDTERGVETVRYEDPALGIYKKLLIKDNRLHGVILVGDASDDHRYKAWLTEATDLTPHRRHLLFPPPAQD
AGLDVAQIPDRETICGCMGVTKGELITAIHQHGIATMTELKDRTRASTGCGSCGNLCARLLKAVAPEFEEEKQKVLCACV
PFPQEKLREIVRSQQLRSVQDVLDVYGNGRGCEICKPALSYMVDMVWCGDHEEDRSARFINDRVHANIQKDGTFSVVPRM
RGGVTTPAELRRIADVAEKYHVPLVKVTGSQRIDLLGVHKSDLPAIWSELGMPSGQAYTKGVRMVKTCVGSQFCRFGTQD
AIAAGVDLETRLENLYSPHKFKMAVVGCPRNCAEATVKDVGLVGNEGSWQVVVGGAAGKSVRKADLLVTVETTDAALEAA
EVFFQYYRENANYLERSYDFVERLGIEKVRKETVYSLPEVRRALLDRLHKSKDKSSDAWLERINPRPTQFVKIQPMETVS
L

Sequences:

>Translated_801_residues
MKKLVVVGNGMAGVACVEQILKYRHEFQITIIGDETHVNYNRILLSSVLAGEKSADDIVLNDIEWYRANHITPRLGLRVA
AIEAGERIVRGTDGSAIPFDKLILATGSTAFVPPVPGTDKQNVHVFRTLDDTRALLEKSRPGRKAVVIGGGLLGLEAARG
LQVQGCDVTVVHLAPTLMERQLDAGGGAYLARKMENLGVKVLLGRQTQAMLGNGRVEGLRFKGGEEVEAELVVVAAGIRP
NIELGRTAGLEVRRGIVVNDYMETSNPDIFAVGECTEHRGQIFGLVAPLMEQGKVLAATITGNRGPVFLGASTAAKLKIM
GVEVFSAGSIDDTERGVETVRYEDPALGIYKKLLIKDNRLHGVILVGDASDDHRYKAWLTEATDLTPHRRHLLFPPPAQD
AGLDVAQIPDRETICGCMGVTKGELITAIHQHGIATMTELKDRTRASTGCGSCGNLCARLLKAVAPEFEEEKQKVLCACV
PFPQEKLREIVRSQQLRSVQDVLDVYGNGRGCEICKPALSYMVDMVWCGDHEEDRSARFINDRVHANIQKDGTFSVVPRM
RGGVTTPAELRRIADVAEKYHVPLVKVTGSQRIDLLGVHKSDLPAIWSELGMPSGQAYTKGVRMVKTCVGSQFCRFGTQD
AIAAGVDLETRLENLYSPHKFKMAVVGCPRNCAEATVKDVGLVGNEGSWQVVVGGAAGKSVRKADLLVTVETTDAALEAA
EVFFQYYRENANYLERSYDFVERLGIEKVRKETVYSLPEVRRALLDRLHKSKDKSSDAWLERINPRPTQFVKIQPMETVS
L
>Mature_801_residues
MKKLVVVGNGMAGVACVEQILKYRHEFQITIIGDETHVNYNRILLSSVLAGEKSADDIVLNDIEWYRANHITPRLGLRVA
AIEAGERIVRGTDGSAIPFDKLILATGSTAFVPPVPGTDKQNVHVFRTLDDTRALLEKSRPGRKAVVIGGGLLGLEAARG
LQVQGCDVTVVHLAPTLMERQLDAGGGAYLARKMENLGVKVLLGRQTQAMLGNGRVEGLRFKGGEEVEAELVVVAAGIRP
NIELGRTAGLEVRRGIVVNDYMETSNPDIFAVGECTEHRGQIFGLVAPLMEQGKVLAATITGNRGPVFLGASTAAKLKIM
GVEVFSAGSIDDTERGVETVRYEDPALGIYKKLLIKDNRLHGVILVGDASDDHRYKAWLTEATDLTPHRRHLLFPPPAQD
AGLDVAQIPDRETICGCMGVTKGELITAIHQHGIATMTELKDRTRASTGCGSCGNLCARLLKAVAPEFEEEKQKVLCACV
PFPQEKLREIVRSQQLRSVQDVLDVYGNGRGCEICKPALSYMVDMVWCGDHEEDRSARFINDRVHANIQKDGTFSVVPRM
RGGVTTPAELRRIADVAEKYHVPLVKVTGSQRIDLLGVHKSDLPAIWSELGMPSGQAYTKGVRMVKTCVGSQFCRFGTQD
AIAAGVDLETRLENLYSPHKFKMAVVGCPRNCAEATVKDVGLVGNEGSWQVVVGGAAGKSVRKADLLVTVETTDAALEAA
EVFFQYYRENANYLERSYDFVERLGIEKVRKETVYSLPEVRRALLDRLHKSKDKSSDAWLERINPRPTQFVKIQPMETVS
L

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=274, Percent_Identity=29.9270072992701, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI65787454, Length=274, Percent_Identity=29.9270072992701, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI226437568, Length=274, Percent_Identity=29.9270072992701, Blast_Score=106, Evalue=8e-23,
Organism=Homo sapiens, GI22202629, Length=186, Percent_Identity=30.1075268817204, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI4757732, Length=212, Percent_Identity=28.7735849056604, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1789765, Length=805, Percent_Identity=35.776397515528, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1789065, Length=311, Percent_Identity=30.8681672025724, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1788892, Length=273, Percent_Identity=28.9377289377289, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=404, Percent_Identity=25.4950495049505, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281359715, Length=275, Percent_Identity=26.1818181818182, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI281359713, Length=275, Percent_Identity=26.1818181818182, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24639250, Length=275, Percent_Identity=26.1818181818182, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI18543267, Length=275, Percent_Identity=26.1818181818182, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24639252, Length=275, Percent_Identity=26.1818181818182, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24639257, Length=275, Percent_Identity=26.1818181818182, Blast_Score=109, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24585130, Length=277, Percent_Identity=23.1046931407942, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28573993, Length=213, Percent_Identity=27.6995305164319, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581020, Length=213, Percent_Identity=27.6995305164319, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 87620; Mature: 87620

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVVVGNGMAGVACVEQILKYRHEFQITIIGDETHVNYNRILLSSVLAGEKSADDIVL
CCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEE
NDIEWYRANHITPRLGLRVAAIEAGERIVRGTDGSAIPFDKLILATGSTAFVPPVPGTDK
CCCHHHHCCCCCHHHCCEEEEECCCCEEEECCCCCCCCHHHEEEECCCCEECCCCCCCCC
QNVHVFRTLDDTRALLEKSRPGRKAVVIGGGLLGLEAARGLQVQGCDVTVVHLAPTLMER
CCEEEEEECHHHHHHHHCCCCCCEEEEEECCEEEHHHHCCCEECCCCEEEEEHHHHHHHH
QLDAGGGAYLARKMENLGVKVLLGRQTQAMLGNGRVEGLRFKGGEEVEAELVVVAAGIRP
HCCCCCCHHHHHHHHHCCEEEEECCCHHHHHCCCCEEEEEECCCCCCCEEEEEEEECCCC
NIELGRTAGLEVRRGIVVNDYMETSNPDIFAVGECTEHRGQIFGLVAPLMEQGKVLAATI
CCCCCCCCCCEECCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEE
TGNRGPVFLGASTAAKLKIMGVEVFSAGSIDDTERGVETVRYEDPALGIYKKLLIKDNRL
ECCCCCEEEECCCCCEEEEEEEEEEECCCCCCCHHCCCEEEECCCHHHHHHHHHCCCCCE
HGVILVGDASDDHRYKAWLTEATDLTPHRRHLLFPPPAQDAGLDVAQIPDRETICGCMGV
EEEEEEECCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCHHCCCCCHHHHHHCCC
TKGELITAIHQHGIATMTELKDRTRASTGCGSCGNLCARLLKAVAPEFEEEKQKVLCACV
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHEEEEEEC
PFPQEKLREIVRSQQLRSVQDVLDVYGNGRGCEICKPALSYMVDMVWCGDHEEDRSARFI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCCCCHHHC
NDRVHANIQKDGTFSVVPRMRGGVTTPAELRRIADVAEKYHVPLVKVTGSQRIDLLGVHK
CHHHEEEECCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCC
SDLPAIWSELGMPSGQAYTKGVRMVKTCVGSQFCRFGTQDAIAAGVDLETRLENLYSPHK
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCE
FKMAVVGCPRNCAEATVKDVGLVGNEGSWQVVVGGAAGKSVRKADLLVTVETTDAALEAA
EEEEEEECCCHHHHHHHHHHCEECCCCCEEEEEECCCCCCEEEEEEEEEEECCHHHHHHH
EVFFQYYRENANYLERSYDFVERLGIEKVRKETVYSLPEVRRALLDRLHKSKDKSSDAWL
HHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
ERINPRPTQFVKIQPMETVSL
HHCCCCCCEEEEECCCHHCCC
>Mature Secondary Structure
MKKLVVVGNGMAGVACVEQILKYRHEFQITIIGDETHVNYNRILLSSVLAGEKSADDIVL
CCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEE
NDIEWYRANHITPRLGLRVAAIEAGERIVRGTDGSAIPFDKLILATGSTAFVPPVPGTDK
CCCHHHHCCCCCHHHCCEEEEECCCCEEEECCCCCCCCHHHEEEECCCCEECCCCCCCCC
QNVHVFRTLDDTRALLEKSRPGRKAVVIGGGLLGLEAARGLQVQGCDVTVVHLAPTLMER
CCEEEEEECHHHHHHHHCCCCCCEEEEEECCEEEHHHHCCCEECCCCEEEEEHHHHHHHH
QLDAGGGAYLARKMENLGVKVLLGRQTQAMLGNGRVEGLRFKGGEEVEAELVVVAAGIRP
HCCCCCCHHHHHHHHHCCEEEEECCCHHHHHCCCCEEEEEECCCCCCCEEEEEEEECCCC
NIELGRTAGLEVRRGIVVNDYMETSNPDIFAVGECTEHRGQIFGLVAPLMEQGKVLAATI
CCCCCCCCCCEECCCEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEE
TGNRGPVFLGASTAAKLKIMGVEVFSAGSIDDTERGVETVRYEDPALGIYKKLLIKDNRL
ECCCCCEEEECCCCCEEEEEEEEEEECCCCCCCHHCCCEEEECCCHHHHHHHHHCCCCCE
HGVILVGDASDDHRYKAWLTEATDLTPHRRHLLFPPPAQDAGLDVAQIPDRETICGCMGV
EEEEEEECCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCCCHHCCCCCHHHHHHCCC
TKGELITAIHQHGIATMTELKDRTRASTGCGSCGNLCARLLKAVAPEFEEEKQKVLCACV
CCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHEEEEEEC
PFPQEKLREIVRSQQLRSVQDVLDVYGNGRGCEICKPALSYMVDMVWCGDHEEDRSARFI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEECCCCCCCCCHHHC
NDRVHANIQKDGTFSVVPRMRGGVTTPAELRRIADVAEKYHVPLVKVTGSQRIDLLGVHK
CHHHEEEECCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECCC
SDLPAIWSELGMPSGQAYTKGVRMVKTCVGSQFCRFGTQDAIAAGVDLETRLENLYSPHK
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHCCCCE
FKMAVVGCPRNCAEATVKDVGLVGNEGSWQVVVGGAAGKSVRKADLLVTVETTDAALEAA
EEEEEEECCCHHHHHHHHHHCEECCCCCEEEEEECCCCCCEEEEEEEEEEECCHHHHHHH
EVFFQYYRENANYLERSYDFVERLGIEKVRKETVYSLPEVRRALLDRLHKSKDKSSDAWL
HHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHH
ERINPRPTQFVKIQPMETVSL
HHCCCCCCEEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]