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The map label for this gene is narB [H]

Identifier: 116619326

GI number: 116619326

Start: 233645

End: 235828

Strand: Direct

Name: narB [H]

Synonym: Acid_0183

Alternate gene names: 116619326

Gene position: 233645-235828 (Clockwise)

Preceding gene: 116619325

Following gene: 116619327

Centisome position: 2.34

GC content: 63.6

Gene sequence:

>2184_bases
ATGAACTTCAAGCGCATGATCGGCCTCGATAACCTCGCAACCGAATACACATTCGCGCGCGATTCCGAAACCGGGTATAC
CGCGGCATCCCGCATCCCCGCGAAATGGATCGCGACCACCTGCGGCTATTGTTCGGTCGGCTGCGGCATGGAGATCGGCG
TCCGCGACAACCACGCCGTCGCCGCCCGCCCGCTCGCCGGGCATCCCGTCAATCGCGGCAAGCTGTGTCCCAAGGGCCTC
TCCGAGCACCACACCATCGATACTGAAAACCGGCTCAAGCGCCCTCTCCTGCGCAAGAAAGGGAAACTCGCCGAGATCGG
CTGGGACGAAGCGACCGCCCTCCTGGTGGAGAAGTTCCGCGCGACGCAGCAGCGCTACGGAGCCAACGCGCTCGGCGTGA
TCAGCACCGGACAATTGGTCACCGAGGAATTCTACACGCTCGGCAAACTGGTGCAGCTGGGCTTCGGCACCAGCAACTAC
GACGGCAACACCACGCTCTGCATGGCCACCGCCGTCTCCGGCTACAAACTCTCCTTCGGCAGCGACGGTCCCCCCGGCGC
ATATGAGGACCTCGAGAAGGCCGACGTTATTCTGCTGATCGGCGCCAACATCGCTGAGAATCACCCAATTCTCTGCCAGT
ACATAGATTCGAACCCGCGCAAGACTCTGATCGTCGCCGATCCGCGCGTCACCAAGACGGCGATGATCGCCGACCTCCAT
CTGCCGCTCAAGCCGCGCTCTGACCTCGCGCTTCTCAACGGCATCGCCCACGTCCTGGTGCGCCACAATCTGATCAACCG
CGATTACATCGATCGCCATACCACGGGCTTCGACGATCTGTCCCGCTTCCTGGAAAATTATCCGCCGGCCAAAGTCTGCG
AGATCACCGGCCTCACGGAGGATCAGCTCTACAAGACGGCCTTCCTCTATGCCCGGGCCAAAGCCGGCTTCATCGGCTGG
ACCATGGGCGTCAACCACAGCACCAAGGGCACGGAGACCGTCAACGCCATCTGCAATCTCGCGCTCCTCACTGGCCACGT
GGGCCGCGCAGGCGCCTCGCCCTTCTCCATCACCGGCCAATGCAACGCCATGGGCACGCGCGAAACCGGTTTCGCCAGCA
GCCTGCCCGGCTACCGCAAGTTCGAGGACGCCCAGGATCGCGCCGAGCTCGCGCGCCTCTGGAAAATCGACGAATCGCGC
ATCCCCCGCAAGCGCGGTCTCGCGTACCCCGATATCGTCGAAGCCGCCGTCGGTGGCACGGTCCGCGCCATGTGGATCAT
CGCCACCAACCCGCTGGTCTCCTTTCCTAACCAGTCCGTGCTGCGCCAGGCGCTCTCAAACCTGGATTTCCTGGTCGTGC
AGGATGGCTATCACCCCACTCCCACCACGGAGTTGGCCGATCTGGTGCTGCCTGCCGCCATATGGGGCGAAAAGGAAGGC
ACCTACACCAACTCCGAGCGCCGCGTCAGCAAGGTCAATAAAGCGGTGGAGCCGGTTGGCGAAGCGCGTTCGGATTTCGA
CATATTCCTCTCCGTTGCGGCCAAGCTCGGCTGCCGCGACGAGCTGTTCCCCGGTTGGCAAACTCCCCGCGACGCATTCG
ACGAATGGCGCGAAGTGTCGCGCGGCCGCCTCTGCGACTACTCGGGAATCACATACGAACTGCTGGCCGAACAGGGCGCC
GTGCAATGGCCATTCCCCGAAGGTGCGACCTTCACGCAGTCATCGCGACTCTATGCCGATGGCCGCTTTCCCACCGCCGA
CGGTCGCGCACGCCTGATTTGCGGAGCTTGGGAAGCCTTCCCGGAGCAGCCCAATCCGCAGTATCCGCTGGTCCTCAACA
CCGGCCGGACCGTGGAACACTGGCACACCCGCACCAAGACCCGCGAAGTGCCCATCCTGGAGCGCCTGTCACCCGCGGCG
TGGCTGGAGATGAATCCGCGCGACGCCGGACGCATGGGCCTGCGCAGTCATGACCGCGTCGATGTGATCTCGCGCCGCGG
CCGTGTCCGGAGCGTGGAACTGCGCCTGACCGAGATTACCGCTCCCGGCCAGGTCTTCATGCCCTTCCACTATTTCGAGA
GCAACGCGAACGAAGTCACCCAAAGCGCGTTCGACCCGGTATCCCGCGAGCCCAACTATAAGCAATGCGCGGTGCGAGTC
GAACGAACTGGAGCAGCCGTATGA

Upstream 100 bases:

>100_bases
TCGCGCTCGCGGGCGAGTGGTCGGGACGCTGGCTCTTCTTCGTCACCGTGGTTCCCAAGAACATGGCCGCGGCCTTCCCG
GCCACGGCGAGGGTGGCTGC

Downstream 100 bases:

>100_bases
AAAAACTCGTAGTCGTCGGCAACGGCATGGCCGGAGTAGCGTGCGTAGAGCAGATTCTCAAGTACCGGCACGAATTTCAA
ATTACCATCATCGGCGACGA

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 727; Mature: 727

Protein sequence:

>727_residues
MNFKRMIGLDNLATEYTFARDSETGYTAASRIPAKWIATTCGYCSVGCGMEIGVRDNHAVAARPLAGHPVNRGKLCPKGL
SEHHTIDTENRLKRPLLRKKGKLAEIGWDEATALLVEKFRATQQRYGANALGVISTGQLVTEEFYTLGKLVQLGFGTSNY
DGNTTLCMATAVSGYKLSFGSDGPPGAYEDLEKADVILLIGANIAENHPILCQYIDSNPRKTLIVADPRVTKTAMIADLH
LPLKPRSDLALLNGIAHVLVRHNLINRDYIDRHTTGFDDLSRFLENYPPAKVCEITGLTEDQLYKTAFLYARAKAGFIGW
TMGVNHSTKGTETVNAICNLALLTGHVGRAGASPFSITGQCNAMGTRETGFASSLPGYRKFEDAQDRAELARLWKIDESR
IPRKRGLAYPDIVEAAVGGTVRAMWIIATNPLVSFPNQSVLRQALSNLDFLVVQDGYHPTPTTELADLVLPAAIWGEKEG
TYTNSERRVSKVNKAVEPVGEARSDFDIFLSVAAKLGCRDELFPGWQTPRDAFDEWREVSRGRLCDYSGITYELLAEQGA
VQWPFPEGATFTQSSRLYADGRFPTADGRARLICGAWEAFPEQPNPQYPLVLNTGRTVEHWHTRTKTREVPILERLSPAA
WLEMNPRDAGRMGLRSHDRVDVISRRGRVRSVELRLTEITAPGQVFMPFHYFESNANEVTQSAFDPVSREPNYKQCAVRV
ERTGAAV

Sequences:

>Translated_727_residues
MNFKRMIGLDNLATEYTFARDSETGYTAASRIPAKWIATTCGYCSVGCGMEIGVRDNHAVAARPLAGHPVNRGKLCPKGL
SEHHTIDTENRLKRPLLRKKGKLAEIGWDEATALLVEKFRATQQRYGANALGVISTGQLVTEEFYTLGKLVQLGFGTSNY
DGNTTLCMATAVSGYKLSFGSDGPPGAYEDLEKADVILLIGANIAENHPILCQYIDSNPRKTLIVADPRVTKTAMIADLH
LPLKPRSDLALLNGIAHVLVRHNLINRDYIDRHTTGFDDLSRFLENYPPAKVCEITGLTEDQLYKTAFLYARAKAGFIGW
TMGVNHSTKGTETVNAICNLALLTGHVGRAGASPFSITGQCNAMGTRETGFASSLPGYRKFEDAQDRAELARLWKIDESR
IPRKRGLAYPDIVEAAVGGTVRAMWIIATNPLVSFPNQSVLRQALSNLDFLVVQDGYHPTPTTELADLVLPAAIWGEKEG
TYTNSERRVSKVNKAVEPVGEARSDFDIFLSVAAKLGCRDELFPGWQTPRDAFDEWREVSRGRLCDYSGITYELLAEQGA
VQWPFPEGATFTQSSRLYADGRFPTADGRARLICGAWEAFPEQPNPQYPLVLNTGRTVEHWHTRTKTREVPILERLSPAA
WLEMNPRDAGRMGLRSHDRVDVISRRGRVRSVELRLTEITAPGQVFMPFHYFESNANEVTQSAFDPVSREPNYKQCAVRV
ERTGAAV
>Mature_727_residues
MNFKRMIGLDNLATEYTFARDSETGYTAASRIPAKWIATTCGYCSVGCGMEIGVRDNHAVAARPLAGHPVNRGKLCPKGL
SEHHTIDTENRLKRPLLRKKGKLAEIGWDEATALLVEKFRATQQRYGANALGVISTGQLVTEEFYTLGKLVQLGFGTSNY
DGNTTLCMATAVSGYKLSFGSDGPPGAYEDLEKADVILLIGANIAENHPILCQYIDSNPRKTLIVADPRVTKTAMIADLH
LPLKPRSDLALLNGIAHVLVRHNLINRDYIDRHTTGFDDLSRFLENYPPAKVCEITGLTEDQLYKTAFLYARAKAGFIGW
TMGVNHSTKGTETVNAICNLALLTGHVGRAGASPFSITGQCNAMGTRETGFASSLPGYRKFEDAQDRAELARLWKIDESR
IPRKRGLAYPDIVEAAVGGTVRAMWIIATNPLVSFPNQSVLRQALSNLDFLVVQDGYHPTPTTELADLVLPAAIWGEKEG
TYTNSERRVSKVNKAVEPVGEARSDFDIFLSVAAKLGCRDELFPGWQTPRDAFDEWREVSRGRLCDYSGITYELLAEQGA
VQWPFPEGATFTQSSRLYADGRFPTADGRARLICGAWEAFPEQPNPQYPLVLNTGRTVEHWHTRTKTREVPILERLSPAA
WLEMNPRDAGRMGLRSHDRVDVISRRGRVRSVELRLTEITAPGQVFMPFHYFESNANEVTQSAFDPVSREPNYKQCAVRV
ERTGAAV

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=700, Percent_Identity=31.1428571428571, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1788534, Length=795, Percent_Identity=32.7044025157233, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI3868719, Length=631, Percent_Identity=24.405705229794, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI3868720, Length=625, Percent_Identity=25.12, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081797, Length=756, Percent_Identity=22.7513227513227, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1787870, Length=751, Percent_Identity=22.9027962716378, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787778, Length=704, Percent_Identity=22.0170454545455, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI171474008, Length=355, Percent_Identity=25.3521126760563, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693196, Length=462, Percent_Identity=24.025974025974, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 80472; Mature: 80472

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKRMIGLDNLATEYTFARDSETGYTAASRIPAKWIATTCGYCSVGCGMEIGVRDNHAV
CCHHHHCCCCHHHHHEEEECCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCEECCCCCCCE
AARPLAGHPVNRGKLCPKGLSEHHTIDTENRLKRPLLRKKGKLAEIGWDEATALLVEKFR
EECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
ATQQRYGANALGVISTGQLVTEEFYTLGKLVQLGFGTSNYDGNTTLCMATAVSGYKLSFG
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEECC
SDGPPGAYEDLEKADVILLIGANIAENHPILCQYIDSNPRKTLIVADPRVTKTAMIADLH
CCCCCCHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEEE
LPLKPRSDLALLNGIAHVLVRHNLINRDYIDRHTTGFDDLSRFLENYPPAKVCEITGLTE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCH
DQLYKTAFLYARAKAGFIGWTMGVNHSTKGTETVNAICNLALLTGHVGRAGASPFSITGQ
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
CNAMGTRETGFASSLPGYRKFEDAQDRAELARLWKIDESRIPRKRGLAYPDIVEAAVGGT
CCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHCCCCHHHHHHHCCCC
VRAMWIIATNPLVSFPNQSVLRQALSNLDFLVVQDGYHPTPTTELADLVLPAAIWGEKEG
EEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
TYTNSERRVSKVNKAVEPVGEARSDFDIFLSVAAKLGCRDELFPGWQTPRDAFDEWREVS
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHH
RGRLCDYSGITYELLAEQGAVQWPFPEGATFTQSSRLYADGRFPTADGRARLICGAWEAF
CCCEECCCCCCHHHHHCCCCEECCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECHHHC
PEQPNPQYPLVLNTGRTVEHWHTRTKTREVPILERLSPAAWLEMNPRDAGRMGLRSHDRV
CCCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCH
DVISRRGRVRSVELRLTEITAPGQVFMPFHYFESNANEVTQSAFDPVSREPNYKQCAVRV
HHHHHCCCEEEEEEEEEEECCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCHHHHEEEE
ERTGAAV
ECCCCCC
>Mature Secondary Structure
MNFKRMIGLDNLATEYTFARDSETGYTAASRIPAKWIATTCGYCSVGCGMEIGVRDNHAV
CCHHHHCCCCHHHHHEEEECCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCEECCCCCCCE
AARPLAGHPVNRGKLCPKGLSEHHTIDTENRLKRPLLRKKGKLAEIGWDEATALLVEKFR
EECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
ATQQRYGANALGVISTGQLVTEEFYTLGKLVQLGFGTSNYDGNTTLCMATAVSGYKLSFG
HHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEECC
SDGPPGAYEDLEKADVILLIGANIAENHPILCQYIDSNPRKTLIVADPRVTKTAMIADLH
CCCCCCHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEEEEEEE
LPLKPRSDLALLNGIAHVLVRHNLINRDYIDRHTTGFDDLSRFLENYPPAKVCEITGLTE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCH
DQLYKTAFLYARAKAGFIGWTMGVNHSTKGTETVNAICNLALLTGHVGRAGASPFSITGQ
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEC
CNAMGTRETGFASSLPGYRKFEDAQDRAELARLWKIDESRIPRKRGLAYPDIVEAAVGGT
CCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHCCCHHHCCHHHCCCCHHHHHHHCCCC
VRAMWIIATNPLVSFPNQSVLRQALSNLDFLVVQDGYHPTPTTELADLVLPAAIWGEKEG
EEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
TYTNSERRVSKVNKAVEPVGEARSDFDIFLSVAAKLGCRDELFPGWQTPRDAFDEWREVS
CCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHH
RGRLCDYSGITYELLAEQGAVQWPFPEGATFTQSSRLYADGRFPTADGRARLICGAWEAF
CCCEECCCCCCHHHHHCCCCEECCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEECHHHC
PEQPNPQYPLVLNTGRTVEHWHTRTKTREVPILERLSPAAWLEMNPRDAGRMGLRSHDRV
CCCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCH
DVISRRGRVRSVELRLTEITAPGQVFMPFHYFESNANEVTQSAFDPVSREPNYKQCAVRV
HHHHHCCCEEEEEEEEEEECCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCHHHHEEEE
ERTGAAV
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8437564 [H]