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The map label for this gene is nuoF2 [H]

Identifier: 116619257

GI number: 116619257

Start: 150798

End: 152111

Strand: Direct

Name: nuoF2 [H]

Synonym: Acid_0113

Alternate gene names: 116619257

Gene position: 150798-152111 (Clockwise)

Preceding gene: 116619256

Following gene: 116619258

Centisome position: 1.51

GC content: 61.72

Gene sequence:

>1314_bases
ATGCTTTTCAACGAACCACATCCGCTGGAAACCAGGGTCCTGACGCGCCGATTTGGTAAGCCGAACTCGGCCGCCATCGA
GGGGTATCTGGCCGACGACGGCTATCAGGGCTTCCTGAAAGCCGCGAAGATGAAACCCGAGGAGATCATCGAGGAAGTCA
AAGTCTCGAACCTTCGCGGACGCGGCGGCGCCGGATTCCCCACCGGCATGAAGTGGAGTTTCGTGCCGCGCACTTCGCCC
AAACCCAAGTACATCGTGGTCAATGGAGATGAGAGCGAGCCCGGCACCTGCAAAGATCGCGTGTTGATCGAAAACGATCC
GCACCAGTTGATCGAAGGCGTCCTGATCGCCGGACTCTCGGTGGATGCGCATGCCGGGTACGTCTACATTCGCGGCGAGT
ATCGCTACCTGATCGACATTATGGAGAAGGCCATCGCCGAGGCTTACGCCAAGGGCTGGCTGGGCAAGAACATTCAGGGT
ACCGGCTTCGATTTCGATTTGTACGCGCACACCGGCGCCGGCGCTTACGAGTGCGGCGAGGAATCGGCGCTGCTCGAATC
GCTTGAAGGCAAGCGCGGCATCCCGCGCATCCGGCCGCCGTTTCCGGCAGTCGCGGGCGCCTTCCAGTGCCCCACGATTC
TCAACAACGTGGAGACGTTCTGCGCGGTGCCGGCAATCCTGCGCGATGGCGGCGCAGCCTTCGCGGCGCTCGGCATTCCC
AAGGCGGGCGGCACCAAGATGACTTGCCTCACCGGTCATGTGAACAAACCGGGCGTCTATGAGCTCAAGCTCGGCTTCCC
GGTGAAGCAGATGATCGAGGAAGTGGGCGGCGGCATGCGCAATGGCAAGAAGTTGAAGGCTTTCATCCCGGGCGGCTCGA
GCTGTCCGGTGCTGACCGCGGCGGAATTGGAGGGCGCGACCATGGATTACGACTCCATGGCATCGCGCAAGAGCATGCTC
GGCTCCGGGGGGGTCATCATCATGGATGAAGACACCTGCATGGTGAAGGCCGCGCTGCGCATCATGCGCTTCTACGCGCA
CGAAAGCTGCGGCTGGTGCATTCCGTGCCGCGAGGGTACAACCTGGCTGAAGAAAATCCTGACGCGCTTCCACGATGGCC
AGGGCACGCGTACCGATATCAATCTGATCGGGGATATCGCCAAGAACATGTTGGGCCGGACCTTCTGTCCGCTGGGCGAT
GCGGCCGCGATGCCGACCATCTCCATCGTGGAGAAGTTCCGCGAGGAGTTCGAAGATCATCTGGCCGGGAAGCCGTGCCG
CTTCGAGCCCGCCAACCGGATGGTGACCGTGTAA

Upstream 100 bases:

>100_bases
CTGGGCGCCGGCGATCCAGATCAATTACGATTTTCACCACTACGTGACTCCCGAGAAGCTGGACCAGCTGCTGGACGGCT
TGAGGAAGAAGCAATAACCA

Downstream 100 bases:

>100_bases
TGGGGACAGATTTGAGAACGCTCCCTTACGGTCGCGGCTCTGATAGGGGAGCGATCTTTTTCTCAATAAAGGTTTGAATG
GCTGATCTGGTAAATCTGAC

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MLFNEPHPLETRVLTRRFGKPNSAAIEGYLADDGYQGFLKAAKMKPEEIIEEVKVSNLRGRGGAGFPTGMKWSFVPRTSP
KPKYIVVNGDESEPGTCKDRVLIENDPHQLIEGVLIAGLSVDAHAGYVYIRGEYRYLIDIMEKAIAEAYAKGWLGKNIQG
TGFDFDLYAHTGAGAYECGEESALLESLEGKRGIPRIRPPFPAVAGAFQCPTILNNVETFCAVPAILRDGGAAFAALGIP
KAGGTKMTCLTGHVNKPGVYELKLGFPVKQMIEEVGGGMRNGKKLKAFIPGGSSCPVLTAAELEGATMDYDSMASRKSML
GSGGVIIMDEDTCMVKAALRIMRFYAHESCGWCIPCREGTTWLKKILTRFHDGQGTRTDINLIGDIAKNMLGRTFCPLGD
AAAMPTISIVEKFREEFEDHLAGKPCRFEPANRMVTV

Sequences:

>Translated_437_residues
MLFNEPHPLETRVLTRRFGKPNSAAIEGYLADDGYQGFLKAAKMKPEEIIEEVKVSNLRGRGGAGFPTGMKWSFVPRTSP
KPKYIVVNGDESEPGTCKDRVLIENDPHQLIEGVLIAGLSVDAHAGYVYIRGEYRYLIDIMEKAIAEAYAKGWLGKNIQG
TGFDFDLYAHTGAGAYECGEESALLESLEGKRGIPRIRPPFPAVAGAFQCPTILNNVETFCAVPAILRDGGAAFAALGIP
KAGGTKMTCLTGHVNKPGVYELKLGFPVKQMIEEVGGGMRNGKKLKAFIPGGSSCPVLTAAELEGATMDYDSMASRKSML
GSGGVIIMDEDTCMVKAALRIMRFYAHESCGWCIPCREGTTWLKKILTRFHDGQGTRTDINLIGDIAKNMLGRTFCPLGD
AAAMPTISIVEKFREEFEDHLAGKPCRFEPANRMVTV
>Mature_437_residues
MLFNEPHPLETRVLTRRFGKPNSAAIEGYLADDGYQGFLKAAKMKPEEIIEEVKVSNLRGRGGAGFPTGMKWSFVPRTSP
KPKYIVVNGDESEPGTCKDRVLIENDPHQLIEGVLIAGLSVDAHAGYVYIRGEYRYLIDIMEKAIAEAYAKGWLGKNIQG
TGFDFDLYAHTGAGAYECGEESALLESLEGKRGIPRIRPPFPAVAGAFQCPTILNNVETFCAVPAILRDGGAAFAALGIP
KAGGTKMTCLTGHVNKPGVYELKLGFPVKQMIEEVGGGMRNGKKLKAFIPGGSSCPVLTAAELEGATMDYDSMASRKSML
GSGGVIIMDEDTCMVKAALRIMRFYAHESCGWCIPCREGTTWLKKILTRFHDGQGTRTDINLIGDIAKNMLGRTFCPLGD
AAAMPTISIVEKFREEFEDHLAGKPCRFEPANRMVTV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=414, Percent_Identity=45.6521739130435, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI260656005, Length=414, Percent_Identity=45.6521739130435, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1788620, Length=417, Percent_Identity=44.1247002398081, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17531727, Length=413, Percent_Identity=46.2469733656174, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19920794, Length=418, Percent_Identity=46.1722488038278, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45550452, Length=375, Percent_Identity=47.7333333333333, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24653819, Length=380, Percent_Identity=39.4736842105263, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=37.7464788732394, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 47515; Mature: 47515

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNEPHPLETRVLTRRFGKPNSAAIEGYLADDGYQGFLKAAKMKPEEIIEEVKVSNLRG
CCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCC
RGGAGFPTGMKWSFVPRTSPKPKYIVVNGDESEPGTCKDRVLIENDPHQLIEGVLIAGLS
CCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHCCE
VDAHAGYVYIRGEYRYLIDIMEKAIAEAYAKGWLGKNIQGTGFDFDLYAHTGAGAYECGE
EECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHCCC
ESALLESLEGKRGIPRIRPPFPAVAGAFQCPTILNNVETFCAVPAILRDGGAAFAALGIP
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
KAGGTKMTCLTGHVNKPGVYELKLGFPVKQMIEEVGGGMRNGKKLKAFIPGGSSCPVLTA
CCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEE
AELEGATMDYDSMASRKSMLGSGGVIIMDEDTCMVKAALRIMRFYAHESCGWCIPCREGT
CCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCH
TWLKKILTRFHDGQGTRTDINLIGDIAKNMLGRTFCPLGDAAAMPTISIVEKFREEFEDH
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
LAGKPCRFEPANRMVTV
HCCCCCCCCCCCCEEEC
>Mature Secondary Structure
MLFNEPHPLETRVLTRRFGKPNSAAIEGYLADDGYQGFLKAAKMKPEEIIEEVKVSNLRG
CCCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCCCC
RGGAGFPTGMKWSFVPRTSPKPKYIVVNGDESEPGTCKDRVLIENDPHQLIEGVLIAGLS
CCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHCCE
VDAHAGYVYIRGEYRYLIDIMEKAIAEAYAKGWLGKNIQGTGFDFDLYAHTGAGAYECGE
EECCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHCCC
ESALLESLEGKRGIPRIRPPFPAVAGAFQCPTILNNVETFCAVPAILRDGGAAFAALGIP
HHHHHHHHCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
KAGGTKMTCLTGHVNKPGVYELKLGFPVKQMIEEVGGGMRNGKKLKAFIPGGSSCPVLTA
CCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEE
AELEGATMDYDSMASRKSMLGSGGVIIMDEDTCMVKAALRIMRFYAHESCGWCIPCREGT
CCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCH
TWLKKILTRFHDGQGTRTDINLIGDIAKNMLGRTFCPLGDAAAMPTISIVEKFREEFEDH
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHHHH
LAGKPCRFEPANRMVTV
HCCCCCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]