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The map label for this gene is nusB

Identifier: 116516227

GI number: 116516227

Start: 394953

End: 395375

Strand: Reverse

Name: nusB

Synonym: SPD_0393

Alternate gene names: 116516227

Gene position: 395375-394953 (Counterclockwise)

Preceding gene: 116516235

Following gene: 116515794

Centisome position: 19.32

GC content: 43.26

Gene sequence:

>423_bases
ATGACTAGTCCACTATTAGAATCTAGACGCCAACTCCGTAAATGCGCTTTTCAAGCTCTCATGAGCCTTGAGTTCGGTAC
GGATGTCGAAACTGCTTGTCGTTTCGCCTATACTCATGATCGTGAAGATACGGATGTACAACTTCCAGCCTTTTTGATAG
ACCTCGTTTCTGGTGTTCAAGCTAAAAAGGAAGAACTAGATAAGCAAATCACTCAGCATTTAAAAGCAGGTTGGACCATT
GAACGCTTAACGCTCGTGGAGAGAAACCTCCTTCGCTTGGGAGTCTTTGAAATCACTTCATTTGACACTCCTCAGCTGGT
TGCTGTTAATGAAGCTATCGAGCTTGCAAAGGACTTCTCCGATCAAAAATCTGCCCGTTTTATCAATGGACTGCTCAGCC
AGTTTGTAACAGAAGAACAATAA

Upstream 100 bases:

>100_bases
GATGTAGAACTCGCTGCTATCAATATTCACGTTGCAGGTATCGTCCCAGATAAAACACCAAAACCAGAATTGAAAGATCT
ATTTGACGAGGACTTCCTCA

Downstream 100 bases:

>100_bases
AAAGAAAAAGTGTTTGATATCTATCAAACACTTTTTCTTTGCCTCCCACTATCTAAAAAAATGATAATAGATATAATTGT
AAACAAAAATCCAGATAGGT

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDTDVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTI
ERLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ

Sequences:

>Translated_140_residues
MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDTDVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTI
ERLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ
>Mature_139_residues
TSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDTDVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIE
RLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ

Specific function: Involved in the transcription termination process

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family

Homologues:

Organism=Escherichia coli, GI1786618, Length=126, Percent_Identity=35.7142857142857, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NUSB_STRP2 (Q04M45)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815903.1
- ProteinModelPortal:   Q04M45
- SMR:   Q04M45
- STRING:   Q04M45
- EnsemblBacteria:   EBSTRT00000020337
- GeneID:   4441487
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0393
- eggNOG:   COG0781
- GeneTree:   EBGT00050000027568
- HOGENOM:   HBG734409
- OMA:   FAYDYDK
- ProtClustDB:   PRK00202
- HAMAP:   MF_00073
- InterPro:   IPR011605
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10
- TIGRFAMs:   TIGR01951

Pfam domain/function: PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 15964; Mature: 15833

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDTDVQLPAFLIDLVSGVQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DQKSARFINGLLSQFVTEEQ
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDTDVQLPAFLIDLVSGVQ
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFDTPQLVAVNEAIELAKDFS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCC
DQKSARFINGLLSQFVTEEQ
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA