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The map label for this gene is argF [H]

Identifier: 116516173

GI number: 116516173

Start: 1950271

End: 1951287

Strand: Direct

Name: argF [H]

Synonym: SPD_1976

Alternate gene names: 116516173

Gene position: 1950271-1951287 (Clockwise)

Preceding gene: 116515902

Following gene: 116517200

Centisome position: 95.32

GC content: 42.28

Gene sequence:

>1017_bases
ATGACAAATTCAGTATTCCAAGGACGCAGCTTCTTAGCAGAAAAAGACTTTACCCGTGCAGAGTTAGAATACCTTATTGG
TCTTTCAGCTCACTTGAAAGATTTGAAAAAACGCAATATTCAACACCACTACCTTGCTGGCAAGAATATCGCTCTCCTAT
TTGAAAAAACATCTACTCGTACTCGTGCAGCCTTTACAACTGCGGCTATCGACCTTGGTGCTCACCCAGAATACCTCGGA
GCAAATGATATTCAGTTGGGTAAAAAAGAATCTACTGAAGATACTGCTAAAGTATTGGGACGTATGTTTGACGGGATTGA
ATTCCGCGGATTCAGCCAACGTATGGTTGAAGAATTGGCAGAATTCTCAGGCGTTCCAGTATGGAACGGTCTAACTGACG
AATGGCACCCAACTCAAATGCTCGCTGACTACTTGACTGTTCAAGAAAACTTCGGTCGCTTGGAAGGCTTGACATTGGTA
TACTGTGGTGATGGACGTAACAACGTTGCCAACAGCTTGCTCGTAACAGGTGCTATCCTTGGTGTCAATGTTCACATCTT
CTCACCAAAAGAACTCTTCCCAGAAAAAGAAATCGTTGAATTGGCAGAAGGATTTGCTAAAGAAAGTGGCGCACATGTTC
TCATCACTGAAGATGCTGATGAAGCAGTTAAAGATGCAGACGTTCTTTACACAGACGTTTGGGTATCAATGGGTGAAGAA
GACAAATTCGCAGAACGTGTAGCTCTTCTTAAACCTTACCAAGTCAATATGGACTTAGTTAAAAAAGCAGGCAATGAAAA
CTTGATCTTCCTACACTGCTTGCCAGCATTCCACGATACTCACACTGTTTATGGTAAAGACGTTGCTGAAAAATTTGGTG
TAGAAGAAATGGAAGTAACAGACGAAGTCTTCCGCAGCAAGTACGCTCGCCACTTCGATCAAGCAGAAAACCGTATGCAC
ACTATCAAAGCTGTTATGGCTGCTACACTTGGTAACCTTTATATTCCTAAAGTATAA

Upstream 100 bases:

>100_bases
ACCTCGTTGTATGTCTATGCCATTTGAACGTGAAGAAGTGTAATCGCTGTTCGGTATTCGTCAATAGAAAATGTAAAAAA
TAGAAAGAGGAAATAATAAA

Downstream 100 bases:

>100_bases
TTTTAGATAATAAACCGTCTACCAACAGCTATGAGGGCTGCGACTAATAGCTTTAGTCCGGTCCTCTTTTATATAATGGT
AATCTATTATTTCTTATAAA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYLAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLG
ANDIQLGKKESTEDTAKVLGRMFDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITEDADEAVKDADVLYTDVWVSMGEE
DKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAFHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMH
TIKAVMAATLGNLYIPKV

Sequences:

>Translated_338_residues
MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYLAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLG
ANDIQLGKKESTEDTAKVLGRMFDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITEDADEAVKDADVLYTDVWVSMGEE
DKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAFHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMH
TIKAVMAATLGNLYIPKV
>Mature_337_residues
TNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYLAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGA
NDIQLGKKESTEDTAKVLGRMFDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLVY
CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITEDADEAVKDADVLYTDVWVSMGEED
KFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAFHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHT
IKAVMAATLGNLYIPKV

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=334, Percent_Identity=40.1197604790419, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI18105007, Length=335, Percent_Identity=24.4776119402985, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1790703, Length=331, Percent_Identity=54.0785498489426, Blast_Score=351, Evalue=3e-98,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=53.5168195718654, Blast_Score=344, Evalue=4e-96,
Organism=Escherichia coli, GI2367364, Length=345, Percent_Identity=28.4057971014493, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994908, Length=378, Percent_Identity=24.8677248677249, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=163, Percent_Identity=30.6748466257669, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6322373, Length=337, Percent_Identity=34.7181008902077, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6322331, Length=335, Percent_Identity=25.9701492537313, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24642586, Length=334, Percent_Identity=28.4431137724551, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 37911; Mature: 37780

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYLAGKNIALLFEKTSTR
CCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCC
TRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMFDGIEFRGFSQRMVEELA
HHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLVYCGDGRNNVANSLLVTGAIL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHEE
GVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITEDADEAVKDADVLYTDVWVSMGEE
EEEEEEECCHHHCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHEEHHHHHCCCC
DKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAFHDTHTVYGKDVAEKFGVEEMEVT
HHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHH
DEVFRSKYARHFDQAENRMHTIKAVMAATLGNLYIPKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHYLAGKNIALLFEKTSTR
CCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCC
TRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMFDGIEFRGFSQRMVEELA
HHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLVYCGDGRNNVANSLLVTGAIL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHEE
GVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITEDADEAVKDADVLYTDVWVSMGEE
EEEEEEECCHHHCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHEEHHHHHCCCC
DKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPAFHDTHTVYGKDVAEKFGVEEMEVT
HHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHH
DEVFRSKYARHFDQAENRMHTIKAVMAATLGNLYIPKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12406748 [H]