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The map label for this gene is tcmA [H]

Identifier: 116496175

GI number: 116496175

Start: 2734459

End: 2736033

Strand: Direct

Name: tcmA [H]

Synonym: LSEI_2753

Alternate gene names: 116496175

Gene position: 2734459-2736033 (Clockwise)

Preceding gene: 116496174

Following gene: 116496177

Centisome position: 94.45

GC content: 48.19

Gene sequence:

>1575_bases
ATGCCTAATGCAACTATGATCACGACACCGAAGAGACATCAGTGGCTGGCATTGACCGCGATGAGCATGGGTGTCTTTAT
GGGATTACTTGACGTCACTGTTGTCAATGTGGCGTTACCCACGATGGTCAACGACTTCAACACGAACTTCACGAATCTCC
AGTGGGTGCTCAACGCCTACACGCTCGTTTATGCCGTGTCATTGTTAATCATGTCTAAACTCGGTGACATGTACGGGCGC
AAAAAAGTATTCCTTGGTTCATTGATTTTGTTCGTGCTGGCTTCGACGGTTAATGGTATGGCCAGTAGCCTAATCGTCTT
GGATATTGGTCGAGGCATTCAGGCTATTGGCGGCGCCGGGATGAATTCACTCTCCATGGCGCTCGTCGCCTCGAACTTTT
CAGGCAATAAACGAGGTGTTGCACTTGGTATCCTTGGCTCCGTTATTGGTATGTCCACCGCATCGGGTCCACTAATTGGC
GGTTTCCTAGTCGAAAATTTCGGGTGGCCGTCTATCTTTTATGTCAATGTGCCTGTCGGTATTGTCGCTGTCATCATGAC
GCTGACGTTTGTTAATGAAACACCAAGTTATGGTCAACACGAACGCATTGATTTTGCTGGCATGCTTTTTTCCGGTCTCG
GTCTTTTTTCTTTAATTTACGGTTTGATTATCAAGGAGGGACATCCCCACTGGTCATGGATGGACATCCGGATCGGTGGT
TGGATCATTGCGGGTTTCATTTTGATAGCACTTTTCATCTGGACCGAAAGCAGAGTCCAACAGCCCATGATGAGTCTTAA
CTTCTTCAAACAGTGTCACTTTGTCGGCACCATATTTGTGGCTTTTGCACTCGGCGCAAGTCTTTACTCTTTCAACACCT
TCTTGACAGCGTTGATGCAAAACTACATTGGCTACTCCGCACTCCAGACCGGTGTTCGGCAACTAACGATCAGCGGCTGG
TCATTGGTCCTTGGCCCGATAAGTGGTTATCTTGGTACGCGATTCTCAAAAAAACGTATGATCAGCGCATCATTATTAGT
TGGCGCATGCGGTTTTTTGGTCATGTTGAATGCGATGGGACCACGGGTCGGTTTTATGGATCTGATGCCCGGCATGCTCA
TGATTGGATTCACTAACGGGATCGTTAGCCCATTGATCAACACGGTTGGAATGGAAGGCGTCGCCCCGCAACAAATGGGG
ATGGCTTCGGGCTTATTGAATATTTTCCGTCAACTCGGGATCGTGATTGGCGTCGTTGGCTTTGGTTTAATGCAGGCAAT
CCAATACGAAAATTATCTGAATACGCATATCCACACCGTGGGGATGCCGAGTCAAATGACAACCGGTATTCACGCTGCCC
TCATTAAGGCAGGTCCATTCTCAGGGCACACAATCGCATGGTCAAAACGTCTAACAGAAACACCATTCGCCCAGAAATTA
CAAAGCGTTGTGGTGCACGCATACGACAACGGCATGGCTGCAGTATTGATTGCAGCGCTTGTCGTGATATTAATTGGCAG
TTTGGCGGCCGCAGTGTTGATGCGAAAAGAATCACATGCTGACGTGCACGCATAG

Upstream 100 bases:

>100_bases
CTTGAGCTAAACATTGCACTTTGCCAGACGAAAAAACAGTGGGTAGCACAAACACGCACTCGCATTTTGCAACAGCAAAC
CACTGAAGGAGGCATGCATC

Downstream 100 bases:

>100_bases
GTATCACTGACCACCCCCGCACCAGACAAAAAAATAAGAAAAACAAAATGGGATACCGGCGAATACTTGCCAGCATCCCA
TTTTGTCGCAACTAACGGCT

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MPNATMITTPKRHQWLALTAMSMGVFMGLLDVTVVNVALPTMVNDFNTNFTNLQWVLNAYTLVYAVSLLIMSKLGDMYGR
KKVFLGSLILFVLASTVNGMASSLIVLDIGRGIQAIGGAGMNSLSMALVASNFSGNKRGVALGILGSVIGMSTASGPLIG
GFLVENFGWPSIFYVNVPVGIVAVIMTLTFVNETPSYGQHERIDFAGMLFSGLGLFSLIYGLIIKEGHPHWSWMDIRIGG
WIIAGFILIALFIWTESRVQQPMMSLNFFKQCHFVGTIFVAFALGASLYSFNTFLTALMQNYIGYSALQTGVRQLTISGW
SLVLGPISGYLGTRFSKKRMISASLLVGACGFLVMLNAMGPRVGFMDLMPGMLMIGFTNGIVSPLINTVGMEGVAPQQMG
MASGLLNIFRQLGIVIGVVGFGLMQAIQYENYLNTHIHTVGMPSQMTTGIHAALIKAGPFSGHTIAWSKRLTETPFAQKL
QSVVVHAYDNGMAAVLIAALVVILIGSLAAAVLMRKESHADVHA

Sequences:

>Translated_524_residues
MPNATMITTPKRHQWLALTAMSMGVFMGLLDVTVVNVALPTMVNDFNTNFTNLQWVLNAYTLVYAVSLLIMSKLGDMYGR
KKVFLGSLILFVLASTVNGMASSLIVLDIGRGIQAIGGAGMNSLSMALVASNFSGNKRGVALGILGSVIGMSTASGPLIG
GFLVENFGWPSIFYVNVPVGIVAVIMTLTFVNETPSYGQHERIDFAGMLFSGLGLFSLIYGLIIKEGHPHWSWMDIRIGG
WIIAGFILIALFIWTESRVQQPMMSLNFFKQCHFVGTIFVAFALGASLYSFNTFLTALMQNYIGYSALQTGVRQLTISGW
SLVLGPISGYLGTRFSKKRMISASLLVGACGFLVMLNAMGPRVGFMDLMPGMLMIGFTNGIVSPLINTVGMEGVAPQQMG
MASGLLNIFRQLGIVIGVVGFGLMQAIQYENYLNTHIHTVGMPSQMTTGIHAALIKAGPFSGHTIAWSKRLTETPFAQKL
QSVVVHAYDNGMAAVLIAALVVILIGSLAAAVLMRKESHADVHA
>Mature_523_residues
PNATMITTPKRHQWLALTAMSMGVFMGLLDVTVVNVALPTMVNDFNTNFTNLQWVLNAYTLVYAVSLLIMSKLGDMYGRK
KVFLGSLILFVLASTVNGMASSLIVLDIGRGIQAIGGAGMNSLSMALVASNFSGNKRGVALGILGSVIGMSTASGPLIGG
FLVENFGWPSIFYVNVPVGIVAVIMTLTFVNETPSYGQHERIDFAGMLFSGLGLFSLIYGLIIKEGHPHWSWMDIRIGGW
IIAGFILIALFIWTESRVQQPMMSLNFFKQCHFVGTIFVAFALGASLYSFNTFLTALMQNYIGYSALQTGVRQLTISGWS
LVLGPISGYLGTRFSKKRMISASLLVGACGFLVMLNAMGPRVGFMDLMPGMLMIGFTNGIVSPLINTVGMEGVAPQQMGM
ASGLLNIFRQLGIVIGVVGFGLMQAIQYENYLNTHIHTVGMPSQMTTGIHAALIKAGPFSGHTIAWSKRLTETPFAQKLQ
SVVVHAYDNGMAAVLIAALVVILIGSLAAAVLMRKESHADVHA

Specific function: Resistance to tetracenomycin C by an active tetracenomycin C efflux system which is probably energized by transmembrane electrochemical gradients [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=403, Percent_Identity=28.0397022332506, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=26.2910798122066, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=25.1833740831296, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1790195, Length=309, Percent_Identity=28.4789644012945, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788392, Length=424, Percent_Identity=26.4150943396226, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790146, Length=177, Percent_Identity=23.728813559322, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=164, Percent_Identity=30.4878048780488, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=174, Percent_Identity=25.8620689655172, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=263, Percent_Identity=25.0950570342205, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=238, Percent_Identity=26.0504201680672, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56324; Mature: 56193

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNATMITTPKRHQWLALTAMSMGVFMGLLDVTVVNVALPTMVNDFNTNFTNLQWVLNAY
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TLVYAVSLLIMSKLGDMYGRKKVFLGSLILFVLASTVNGMASSLIVLDIGRGIQAIGGAG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCCC
MNSLSMALVASNFSGNKRGVALGILGSVIGMSTASGPLIGGFLVENFGWPSIFYVNVPVG
CCHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECCHH
IVAVIMTLTFVNETPSYGQHERIDFAGMLFSGLGLFSLIYGLIIKEGHPHWSWMDIRIGG
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECH
WIIAGFILIALFIWTESRVQQPMMSLNFFKQCHFVGTIFVAFALGASLYSFNTFLTALMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYIGYSALQTGVRQLTISGWSLVLGPISGYLGTRFSKKRMISASLLVGACGFLVMLNAMG
HHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PRVGFMDLMPGMLMIGFTNGIVSPLINTVGMEGVAPQQMGMASGLLNIFRQLGIVIGVVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FGLMQAIQYENYLNTHIHTVGMPSQMTTGIHAALIKAGPFSGHTIAWSKRLTETPFAQKL
HHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCHHHHHH
QSVVVHAYDNGMAAVLIAALVVILIGSLAAAVLMRKESHADVHA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PNATMITTPKRHQWLALTAMSMGVFMGLLDVTVVNVALPTMVNDFNTNFTNLQWVLNAY
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TLVYAVSLLIMSKLGDMYGRKKVFLGSLILFVLASTVNGMASSLIVLDIGRGIQAIGGAG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCCC
MNSLSMALVASNFSGNKRGVALGILGSVIGMSTASGPLIGGFLVENFGWPSIFYVNVPVG
CCHHHHHHHEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEECCHH
IVAVIMTLTFVNETPSYGQHERIDFAGMLFSGLGLFSLIYGLIIKEGHPHWSWMDIRIGG
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECH
WIIAGFILIALFIWTESRVQQPMMSLNFFKQCHFVGTIFVAFALGASLYSFNTFLTALMQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NYIGYSALQTGVRQLTISGWSLVLGPISGYLGTRFSKKRMISASLLVGACGFLVMLNAMG
HHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PRVGFMDLMPGMLMIGFTNGIVSPLINTVGMEGVAPQQMGMASGLLNIFRQLGIVIGVVG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FGLMQAIQYENYLNTHIHTVGMPSQMTTGIHAALIKAGPFSGHTIAWSKRLTETPFAQKL
HHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHCCCCHHHHHH
QSVVVHAYDNGMAAVLIAALVVILIGSLAAAVLMRKESHADVHA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1592819 [H]