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The map label for this gene is insF1 [H]

Identifier: 116495797

GI number: 116495797

Start: 2345453

End: 2346076

Strand: Direct

Name: insF1 [H]

Synonym: LSEI_2345

Alternate gene names: 116495797

Gene position: 2345453-2346076 (Clockwise)

Preceding gene: 116495796

Following gene: 116495799

Centisome position: 81.01

GC content: 43.75

Gene sequence:

>624_bases
ATGAAGGAACTGGATATCCACTCCGTTACCGTCAATAAGTGGAAAGCGGCTTCGGCTTCCAAAACCAAGGTTGAACAGCG
TCCCAACTTGCTTAAGCAGGATTTCTCGACCACTGGTTTAAATCAAAAATGGACCGCTGATATGACCTATATTCAAACGA
AGCGTAATGGCTGGTGTTACTTATCAACCATCATGGACCTGCACTCACGACGGATTATCGGCTATTCGTTCTCAAAAAAG
ATGGATACTGATTTAGTCTTAAAGACCCTTGAAAGCGCGGTTAAAAATCGAACCATTACTGGGGACCTGATTATCCATAC
GGATTTAGGATCACAGTATACCAGCGATGATTACAATCAACGTTTAACTGAGCTACATATCCGCCACTCATACAGCCGTA
AGGGTTGTCCGTATGATAATGCGCCAATGGAATCCTTTCACGCTTCCCTCAAAAAGGAATGTGTTTATCCAGTGCCGGTC
TTTGAAGATTATGAAACTGCCGCTGCCGTCCTTTTTGAATATGTGCATGCTTTCTACAATAGGAAGAGAATTCATAGTTC
ACTGGGCTACCAGACCCCCTTACAAGTTAAAATTGCAACACTTACGAGCCAAATGGCCGCCTGA

Upstream 100 bases:

>100_bases
ATTTAAGCAGCGATACGGTGTTATGAAGATCCACCATGAATTGAATCTGGAACTTCAACCACTGCAGCTTCGGTGCAGTC
CATGACGGATTTCCCGGCTC

Downstream 100 bases:

>100_bases
TTTAATGCTTTCCAGGGTTCAAATAAGTTATTAATCGCAATTAATGATTTATTTGAGCTGTGGAAGGTAAACGCGGTTCT
GAATGTCTCTTAAAATCTGT

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKELDIHSVTVNKWKAASASKTKVEQRPNLLKQDFSTTGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKK
MDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPV
FEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLQVKIATLTSQMAA

Sequences:

>Translated_207_residues
MKELDIHSVTVNKWKAASASKTKVEQRPNLLKQDFSTTGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKK
MDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPV
FEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLQVKIATLTSQMAA
>Mature_207_residues
MKELDIHSVTVNKWKAASASKTKVEQRPNLLKQDFSTTGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKK
MDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPV
FEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLQVKIATLTSQMAA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=155, Percent_Identity=36.1290322580645, Blast_Score=104, Evalue=7e-23,
Organism=Escherichia coli, GI1788405, Length=174, Percent_Identity=43.6781609195402, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1787263, Length=174, Percent_Identity=43.6781609195402, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1786753, Length=174, Percent_Identity=43.6781609195402, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1786570, Length=174, Percent_Identity=43.6781609195402, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1786490, Length=174, Percent_Identity=43.6781609195402, Blast_Score=144, Evalue=3e-36,
Organism=Escherichia coli, GI1789981, Length=174, Percent_Identity=35.0574712643678, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 23799; Mature: 23799

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELDIHSVTVNKWKAASASKTKVEQRPNLLKQDFSTTGLNQKWTADMTYIQTKRNGWCY
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCEE
LSTIMDLHSRRIIGYSFSKKMDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQ
HHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
RLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYN
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RKRIHSSLGYQTPLQVKIATLTSQMAA
HHHHHHHCCCCCCCEEEHHHHHHHHCC
>Mature Secondary Structure
MKELDIHSVTVNKWKAASASKTKVEQRPNLLKQDFSTTGLNQKWTADMTYIQTKRNGWCY
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCEE
LSTIMDLHSRRIIGYSFSKKMDTDLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQ
HHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
RLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYN
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RKRIHSSLGYQTPLQVKIATLTSQMAA
HHHHHHHCCCCCCCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]