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The map label for this gene is ycnB [H]

Identifier: 116495683

GI number: 116495683

Start: 2222922

End: 2224379

Strand: Direct

Name: ycnB [H]

Synonym: LSEI_2230

Alternate gene names: 116495683

Gene position: 2222922-2224379 (Clockwise)

Preceding gene: 116495682

Following gene: 116495684

Centisome position: 76.78

GC content: 49.04

Gene sequence:

>1458_bases
TTGTTGACAACAACAGACGTTAATGGTCATCCATATAATCGCTTGCTGCTTGTGATTACCTTGTTGATCGGTACTTTTAC
AACATTCTTGACGCAAACGATTTTGACCACGGCTTACCCGACGCTGATGGCGGATTTTCACATCAGCGCGAGTGCAGTCC
AATGGCTGACCACTGGCTTCATGCTGATTATGGGTATTATGATCCCGGTTTCCGCATGGCTGCTGGATAAATTCAATGTT
AAGTATCTCTACTTAACAGCTATGACAATCTTTTTCCTTGGTTCCTTGATTTGTTGGTTAGCACCAGATTTTCAAGTTCT
GCTGGCTGGTCGGCTCACGCAGGCAATTGGCGTTGGTATGTCAATGCCGACTTTCCAAACAGTCATGTTCACGATTTTCC
CACCTGGCAAACGTGGTAGTGCCATGGGCCTAGCTGGGATTGTTATTGGCCTAGCTCCTGCCATCGGCCCGACGCTTTCT
GGTTGGATTCTGCTTAATCATACTTGGCGGGCTCTATTTGCCGTTATTATGCCAATTGCTGCTGGGGTCGTGCTGCTGAC
CATCTATAGCATGCGAAAAGTTTTGCCGACCTCGAATCCAAAAATTGATATTCCTTCCATTATTGCTTCGACTTTGGGTT
TTGGCGGCTTGCTTTATGGCTTCTCCACAGTTGGTGATGCTGGTTGGACCGATTCCCAAGTACTGATTAGCTTAGCCATC
GGCATTATTTTCGTTGCCTTATTCTGCGTGCGCCAGTTACGCCTCGAAACGCCTCTGCTAGAACTGCGCGTTTTCAAATC
ACGCACGTATACATTGAGCGTCATTTTAACAGCGGTGGCGTTTATGTCGATGGTTGGTGTAGAAATGGTCTTGCCAATGT
ACATCCAAACGATCCGTGGTGAATCTGCCTTTCACTCTGGGATGATCCTTTTTCCCGGAGCCATCATGATGGGGATTATG
AGTCCAATTACCGGCCGGATTTTCGACAAAATTGGGGCCAAGCGGCTGGCTGTTGCCGGTTTGTTCCTGCTAGTTGCCGG
ATCGCTGCCGCTGGTCACCTTAACTGCTAACACGCCGATTATCTATATCACGACCATTTACACAGTGCGCATGTTCGGCA
TTACCATGGTCACGATGCCGGTCACCACCGCTGGTATGAACGCCTTGCCAACACACCTGATCAATCACGGGACGGCGGTC
AATAACACGGTTCGGCAAGTCGCCGCCTCAATTGGCACCGCAATACTGGTGTCAGTATTATCAGTAGTCACTAAAGATGC
AACGCCAGCCGCTAGTGGTCGACTGCTTGACCCGATCCATTACACCAACGCTATGAACAATGCAGTCATTGCCGGTTATC
GCGCGGCTTTTGCCGTCTCACTAGTCATGGCAGCGCTTGGTCTCGTTCTCAGCTTATTTTTGAAAACAGACGTCATTGAT
CGGAGGGACGCCTCATGA

Upstream 100 bases:

>100_bases
ATAAACTAAACCCTAATGACAGCAACGATTTCATTATTTACCCAAAGCCGTTGAGGACGTGCTAGACTGGATTATAACGA
TTTTAAGAAAGGACACGATA

Downstream 100 bases:

>100_bases
TTATCTTGTTATTGATCGTCGGTGCGCTTGCCTTTTTCTGGGCTGTTGTGTTAATGCAACCAAGTCGGCGCCGTCAATTA
ACGATCTGGGTAAGCGCGGG

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MLTTTDVNGHPYNRLLLVITLLIGTFTTFLTQTILTTAYPTLMADFHISASAVQWLTTGFMLIMGIMIPVSAWLLDKFNV
KYLYLTAMTIFFLGSLICWLAPDFQVLLAGRLTQAIGVGMSMPTFQTVMFTIFPPGKRGSAMGLAGIVIGLAPAIGPTLS
GWILLNHTWRALFAVIMPIAAGVVLLTIYSMRKVLPTSNPKIDIPSIIASTLGFGGLLYGFSTVGDAGWTDSQVLISLAI
GIIFVALFCVRQLRLETPLLELRVFKSRTYTLSVILTAVAFMSMVGVEMVLPMYIQTIRGESAFHSGMILFPGAIMMGIM
SPITGRIFDKIGAKRLAVAGLFLLVAGSLPLVTLTANTPIIYITTIYTVRMFGITMVTMPVTTAGMNALPTHLINHGTAV
NNTVRQVAASIGTAILVSVLSVVTKDATPAASGRLLDPIHYTNAMNNAVIAGYRAAFAVSLVMAALGLVLSLFLKTDVID
RRDAS

Sequences:

>Translated_485_residues
MLTTTDVNGHPYNRLLLVITLLIGTFTTFLTQTILTTAYPTLMADFHISASAVQWLTTGFMLIMGIMIPVSAWLLDKFNV
KYLYLTAMTIFFLGSLICWLAPDFQVLLAGRLTQAIGVGMSMPTFQTVMFTIFPPGKRGSAMGLAGIVIGLAPAIGPTLS
GWILLNHTWRALFAVIMPIAAGVVLLTIYSMRKVLPTSNPKIDIPSIIASTLGFGGLLYGFSTVGDAGWTDSQVLISLAI
GIIFVALFCVRQLRLETPLLELRVFKSRTYTLSVILTAVAFMSMVGVEMVLPMYIQTIRGESAFHSGMILFPGAIMMGIM
SPITGRIFDKIGAKRLAVAGLFLLVAGSLPLVTLTANTPIIYITTIYTVRMFGITMVTMPVTTAGMNALPTHLINHGTAV
NNTVRQVAASIGTAILVSVLSVVTKDATPAASGRLLDPIHYTNAMNNAVIAGYRAAFAVSLVMAALGLVLSLFLKTDVID
RRDAS
>Mature_485_residues
MLTTTDVNGHPYNRLLLVITLLIGTFTTFLTQTILTTAYPTLMADFHISASAVQWLTTGFMLIMGIMIPVSAWLLDKFNV
KYLYLTAMTIFFLGSLICWLAPDFQVLLAGRLTQAIGVGMSMPTFQTVMFTIFPPGKRGSAMGLAGIVIGLAPAIGPTLS
GWILLNHTWRALFAVIMPIAAGVVLLTIYSMRKVLPTSNPKIDIPSIIASTLGFGGLLYGFSTVGDAGWTDSQVLISLAI
GIIFVALFCVRQLRLETPLLELRVFKSRTYTLSVILTAVAFMSMVGVEMVLPMYIQTIRGESAFHSGMILFPGAIMMGIM
SPITGRIFDKIGAKRLAVAGLFLLVAGSLPLVTLTANTPIIYITTIYTVRMFGITMVTMPVTTAGMNALPTHLINHGTAV
NNTVRQVAASIGTAILVSVLSVVTKDATPAASGRLLDPIHYTNAMNNAVIAGYRAAFAVSLVMAALGLVLSLFLKTDVID
RRDAS

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=405, Percent_Identity=27.9012345679012, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081983, Length=431, Percent_Identity=25.9860788863109, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788710, Length=402, Percent_Identity=28.8557213930348, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1790195, Length=406, Percent_Identity=25.1231527093596, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=24.6913580246914, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI48994889, Length=179, Percent_Identity=28.4916201117318, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=330, Percent_Identity=23.030303030303, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52070; Mature: 52070

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTTDVNGHPYNRLLLVITLLIGTFTTFLTQTILTTAYPTLMADFHISASAVQWLTTGF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MLIMGIMIPVSAWLLDKFNVKYLYLTAMTIFFLGSLICWLAPDFQVLLAGRLTQAIGVGM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
SMPTFQTVMFTIFPPGKRGSAMGLAGIVIGLAPAIGPTLSGWILLNHTWRALFAVIMPIA
CCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHH
AGVVLLTIYSMRKVLPTSNPKIDIPSIIASTLGFGGLLYGFSTVGDAGWTDSQVLISLAI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHH
GIIFVALFCVRQLRLETPLLELRVFKSRTYTLSVILTAVAFMSMVGVEMVLPMYIQTIRG
HHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ESAFHSGMILFPGAIMMGIMSPITGRIFDKIGAKRLAVAGLFLLVAGSLPLVTLTANTPI
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCE
IYITTIYTVRMFGITMVTMPVTTAGMNALPTHLINHGTAVNNTVRQVAASIGTAILVSVL
EEEHHHHHHHHHHHHHEEECCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SVVTKDATPAASGRLLDPIHYTNAMNNAVIAGYRAAFAVSLVMAALGLVLSLFLKTDVID
HHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RRDAS
CCCCC
>Mature Secondary Structure
MLTTTDVNGHPYNRLLLVITLLIGTFTTFLTQTILTTAYPTLMADFHISASAVQWLTTGF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MLIMGIMIPVSAWLLDKFNVKYLYLTAMTIFFLGSLICWLAPDFQVLLAGRLTQAIGVGM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
SMPTFQTVMFTIFPPGKRGSAMGLAGIVIGLAPAIGPTLSGWILLNHTWRALFAVIMPIA
CCCHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHH
AGVVLLTIYSMRKVLPTSNPKIDIPSIIASTLGFGGLLYGFSTVGDAGWTDSQVLISLAI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHHHHHH
GIIFVALFCVRQLRLETPLLELRVFKSRTYTLSVILTAVAFMSMVGVEMVLPMYIQTIRG
HHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ESAFHSGMILFPGAIMMGIMSPITGRIFDKIGAKRLAVAGLFLLVAGSLPLVTLTANTPI
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCE
IYITTIYTVRMFGITMVTMPVTTAGMNALPTHLINHGTAVNNTVRQVAASIGTAILVSVL
EEEHHHHHHHHHHHHHEEECCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
SVVTKDATPAASGRLLDPIHYTNAMNNAVIAGYRAAFAVSLVMAALGLVLSLFLKTDVID
HHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RRDAS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]