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The map label for this gene is yclM [H]

Identifier: 116495609

GI number: 116495609

Start: 2141988

End: 2143334

Strand: Direct

Name: yclM [H]

Synonym: LSEI_2152

Alternate gene names: 116495609

Gene position: 2141988-2143334 (Clockwise)

Preceding gene: 116495608

Following gene: 116495617

Centisome position: 73.98

GC content: 48.92

Gene sequence:

>1347_bases
ATGAAGGTCGTTAAATTTGGCGGTAGTTCCGTGGCCGATGCCGGTCAATTCCAGAAAGTTAAAGCCATAATCGATCAGGA
TGTGAATCGGCAAATCGTCGTGATTAGCGCAGCTGGAAAATATGGTAATGCTACCCGAAAATTAACTGATACGCTCTATT
TAATTGCTGACGGCATGGAAGCCGGTCGAGATGTCGAACCGCTGTTTAATCAGGTCTTGGCTCGGCTAGAGGCCATCAAC
ATTGCGCTGCAGCTCAATGTGCCGCTCATCAAACTGTTGCATACAATTCGCCATCATCTGGCCATTCGCTATTCTCGAGA
CTATCTGGTCTCTCGCGGTGAGTTCTTGACAGCCCACTTAATGGCAGCCTACTTAGGCTATACTTTTGTGGACGCCGCCA
ATCTTTTGTTTTTCAATAAAGCAGGCGCCATTGATGAAGCCAAAACTCAGACTGCTTATCAACAACTAGCCAGCCAGCAT
GGTATCGTCGTTCCCGGTTTTTATGGTCGCGACGCAGACGGACATGTCAAGCTGATGCCGCGCGGCGGTTCTGACATTTC
TGGTGCATGGCTAGCACGCTTGGCCCACGCTGATCTCTATGAAAATTGGACCGATGTTTCAGGCATTAAAATGGCCGATC
CGCGTATCATTAAAGACGCCGACAGTATTGATGTGATGAGTTACGACGAATTACGCGAACTCACCTACATGGGATTTAGT
GTTTTCCAAGAAGAGGCGGTGCAACCGGTCCGTGAATGTGGCATCCCGACGCGTATTTTGAACACCAATGCGCCAGAAGT
CCCAGGCACGCTGATCATTCATGATGATGACCCCGCCAATGATCTCAAAACAATCACTGGTATTGCTGGCCGTAAACATT
ATTTGGTCATCACGGTCCGCAAGTACCAGCTGGCCTTGCACTTAGAAGTCATTCAACATGCGCTCGGAGTAGTTGCAGCG
CACCATCTCGTTGTCGATTATCTGCCCACGGGCATTGACAGTTTCAGTCTGCTCATTCGTGAACCGCGTCGTCAAGTCTC
GGTACAGGAGCTGACCGCTGAAATCGCCGCCGCCTGTCAGCCAGACAAAATGGAAGTCACCGAAAATGTTGCGTTAATCG
CCATGGTTTCTCGTAAACTTCGACAACGACCAGCCATTGCCGGAAAAGTCCTCGCTTATCTGGATGATAACCTGCTCAAC
GTACAACTCGTTAGTCAGACCAATGACGATATCAATTTGCTAATCGGCGTACATGATCTTGATTACGACAAAGCTGTTCG
GGTCATTTATAGCAGTATTCATCAAAACTTTCAGCGCCCGGCGCCTTATATTGAGGGGATTGCCTAG

Upstream 100 bases:

>100_bases
GCGTTTTGGCCATTGATGCCGATGCTTTTATGGCAGCAATCGGAGACAGTGAGTTGGCTGCTAAAGACGTCCTTCACCAG
CAAGATCAGGAGGTGATCGG

Downstream 100 bases:

>100_bases
CGCGTTACAAGTCTTGAATCGCATTTAATAAAAAAGCATCCTTTCCGCAAAAATTTACCGGAAAGGATGCTTTTTTGTTA
ATCCAACCTTTTGTCACTAG

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKVVKFGGSSVADAGQFQKVKAIIDQDVNRQIVVISAAGKYGNATRKLTDTLYLIADGMEAGRDVEPLFNQVLARLEAIN
IALQLNVPLIKLLHTIRHHLAIRYSRDYLVSRGEFLTAHLMAAYLGYTFVDAANLLFFNKAGAIDEAKTQTAYQQLASQH
GIVVPGFYGRDADGHVKLMPRGGSDISGAWLARLAHADLYENWTDVSGIKMADPRIIKDADSIDVMSYDELRELTYMGFS
VFQEEAVQPVRECGIPTRILNTNAPEVPGTLIIHDDDPANDLKTITGIAGRKHYLVITVRKYQLALHLEVIQHALGVVAA
HHLVVDYLPTGIDSFSLLIREPRRQVSVQELTAEIAAACQPDKMEVTENVALIAMVSRKLRQRPAIAGKVLAYLDDNLLN
VQLVSQTNDDINLLIGVHDLDYDKAVRVIYSSIHQNFQRPAPYIEGIA

Sequences:

>Translated_448_residues
MKVVKFGGSSVADAGQFQKVKAIIDQDVNRQIVVISAAGKYGNATRKLTDTLYLIADGMEAGRDVEPLFNQVLARLEAIN
IALQLNVPLIKLLHTIRHHLAIRYSRDYLVSRGEFLTAHLMAAYLGYTFVDAANLLFFNKAGAIDEAKTQTAYQQLASQH
GIVVPGFYGRDADGHVKLMPRGGSDISGAWLARLAHADLYENWTDVSGIKMADPRIIKDADSIDVMSYDELRELTYMGFS
VFQEEAVQPVRECGIPTRILNTNAPEVPGTLIIHDDDPANDLKTITGIAGRKHYLVITVRKYQLALHLEVIQHALGVVAA
HHLVVDYLPTGIDSFSLLIREPRRQVSVQELTAEIAAACQPDKMEVTENVALIAMVSRKLRQRPAIAGKVLAYLDDNLLN
VQLVSQTNDDINLLIGVHDLDYDKAVRVIYSSIHQNFQRPAPYIEGIA
>Mature_448_residues
MKVVKFGGSSVADAGQFQKVKAIIDQDVNRQIVVISAAGKYGNATRKLTDTLYLIADGMEAGRDVEPLFNQVLARLEAIN
IALQLNVPLIKLLHTIRHHLAIRYSRDYLVSRGEFLTAHLMAAYLGYTFVDAANLLFFNKAGAIDEAKTQTAYQQLASQH
GIVVPGFYGRDADGHVKLMPRGGSDISGAWLARLAHADLYENWTDVSGIKMADPRIIKDADSIDVMSYDELRELTYMGFS
VFQEEAVQPVRECGIPTRILNTNAPEVPGTLIIHDDDPANDLKTITGIAGRKHYLVITVRKYQLALHLEVIQHALGVVAA
HHLVVDYLPTGIDSFSLLIREPRRQVSVQELTAEIAAACQPDKMEVTENVALIAMVSRKLRQRPAIAGKVLAYLDDNLLN
VQLVSQTNDDINLLIGVHDLDYDKAVRVIYSSIHQNFQRPAPYIEGIA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=471, Percent_Identity=25.9023354564756, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790455, Length=460, Percent_Identity=25, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790376, Length=286, Percent_Identity=28.3216783216783, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320893, Length=489, Percent_Identity=25.1533742331288, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49571; Mature: 49571

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVKFGGSSVADAGQFQKVKAIIDQDVNRQIVVISAAGKYGNATRKLTDTLYLIADGME
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHEEEEEECCCC
AGRDVEPLFNQVLARLEAINIALQLNVPLIKLLHTIRHHLAIRYSRDYLVSRGEFLTAHL
CCCCHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHEEEECCHHHHCCCHHHHHHH
MAAYLGYTFVDAANLLFFNKAGAIDEAKTQTAYQQLASQHGIVVPGFYGRDADGHVKLMP
HHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEE
RGGSDISGAWLARLAHADLYENWTDVSGIKMADPRIIKDADSIDVMSYDELRELTYMGFS
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHHHHHHHHH
VFQEEAVQPVRECGIPTRILNTNAPEVPGTLIIHDDDPANDLKTITGIAGRKHYLVITVR
HHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEEEE
KYQLALHLEVIQHALGVVAAHHLVVDYLPTGIDSFSLLIREPRRQVSVQELTAEIAAACQ
EHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCC
PDKMEVTENVALIAMVSRKLRQRPAIAGKVLAYLDDNLLNVQLVSQTNDDINLLIGVHDL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEECC
DYDKAVRVIYSSIHQNFQRPAPYIEGIA
CHHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MKVVKFGGSSVADAGQFQKVKAIIDQDVNRQIVVISAAGKYGNATRKLTDTLYLIADGME
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHEEEEEECCCC
AGRDVEPLFNQVLARLEAINIALQLNVPLIKLLHTIRHHLAIRYSRDYLVSRGEFLTAHL
CCCCHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHEEEECCHHHHCCCHHHHHHH
MAAYLGYTFVDAANLLFFNKAGAIDEAKTQTAYQQLASQHGIVVPGFYGRDADGHVKLMP
HHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEE
RGGSDISGAWLARLAHADLYENWTDVSGIKMADPRIIKDADSIDVMSYDELRELTYMGFS
CCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCEECCCCCCCCCCHHHHHHHHHHHHH
VFQEEAVQPVRECGIPTRILNTNAPEVPGTLIIHDDDPANDLKTITGIAGRKHYLVITVR
HHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEEEE
KYQLALHLEVIQHALGVVAAHHLVVDYLPTGIDSFSLLIREPRRQVSVQELTAEIAAACQ
EHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCC
PDKMEVTENVALIAMVSRKLRQRPAIAGKVLAYLDDNLLNVQLVSQTNDDINLLIGVHDL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEEECC
DYDKAVRVIYSSIHQNFQRPAPYIEGIA
CHHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]