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The map label for this gene is dagK [H]

Identifier: 116495464

GI number: 116495464

Start: 1972615

End: 1973496

Strand: Direct

Name: dagK [H]

Synonym: LSEI_1998

Alternate gene names: 116495464

Gene position: 1972615-1973496 (Clockwise)

Preceding gene: 116495462

Following gene: 116495465

Centisome position: 68.13

GC content: 51.02

Gene sequence:

>882_bases
ATGCTCAGCTATACGATTATCTACAACCCGGCGTCCGGGCATTCTAAAGGACCAACTGCTGCCGAACAACTCAAAACGAA
ACTGGAAGATCGCCAGCATCAGGTCACGATGGCGCCAACCAAAAGTGCCGATGACGCGCGCGAGTTCGCCTATCACGCCC
AAGCAGACATTGTGGTGGCTGTCGGTGGTGACGGCACCATCAATCAAGTTGTCGCTGGTCTCGCACCGCGCAAAAATCCG
CCACTGTTGGCCATTTTGCCTGAAGGAACCGTTAATAATCTCGCCAAGGTTTTGCATATTCCGTTACTGTTGCCGTTAGC
CATCAAAAACATTCTGGAAGCTGCCCCACAGCCACTCGACGTTGCTCAGGTCAATAATCACTACATGGTCAGCACGTTGA
CCCTCGGTGTTTTAGCAAATGCGGCTTTATCTGTGACCCAGAAAGAAAAACAACACTTCGGCCCGATCATCTACCTCATG
AAGGGCTTTAAAGTTCTGGCTCAACATCAACATTGGATCCTACAGTTGGATAGCGCCGATGGTAAGTGGCAAAAAGACAC
GCAGTTCCTATTAATCACGATGACAAACTCAGTCGGCGGCTTCACCAACGCCGTCCCTGACGCTGCCCCTGATGATGGTC
GGCTGCACGTTTTCCTCGCCCCCAAACTGACCTTGTGGCGATCACTACTAGCAATCCCGTACTTCATCACCGGCAACTTC
CAAAAACTGCCCGGCATGACCTACTTTGCGACCAAAGAGTTGACCATCAAAACACCGAAAACCTTACAAAGCAGGATTGA
CGGCGATCCTAGTACCAAAACGCCATTGACGCTGAAGGTGATTAGCGATCATATTCAGGTCCTGGCACCGAATGTTGGGT
AG

Upstream 100 bases:

>100_bases
TCAAAATCATACAGGCGGTCGTCTTTTTATCCTTAAAATTATCACCGGCCAGCATGCGCTATCGTTCACCGTGATAAACT
AGTTAATAGGAGGTGCCGCC

Downstream 100 bases:

>100_bases
ATTTGGGCTACCGTGTTACACTTGGTCTAATGAAACGAAAGGGGTGAATCGCCATGCGGTTACGTCAGGCGCACCATGAA
TGATGATCTGCTAGCACCGA

Product: diacylglycerol kinase family lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MLSYTIIYNPASGHSKGPTAAEQLKTKLEDRQHQVTMAPTKSADDAREFAYHAQADIVVAVGGDGTINQVVAGLAPRKNP
PLLAILPEGTVNNLAKVLHIPLLLPLAIKNILEAAPQPLDVAQVNNHYMVSTLTLGVLANAALSVTQKEKQHFGPIIYLM
KGFKVLAQHQHWILQLDSADGKWQKDTQFLLITMTNSVGGFTNAVPDAAPDDGRLHVFLAPKLTLWRSLLAIPYFITGNF
QKLPGMTYFATKELTIKTPKTLQSRIDGDPSTKTPLTLKVISDHIQVLAPNVG

Sequences:

>Translated_293_residues
MLSYTIIYNPASGHSKGPTAAEQLKTKLEDRQHQVTMAPTKSADDAREFAYHAQADIVVAVGGDGTINQVVAGLAPRKNP
PLLAILPEGTVNNLAKVLHIPLLLPLAIKNILEAAPQPLDVAQVNNHYMVSTLTLGVLANAALSVTQKEKQHFGPIIYLM
KGFKVLAQHQHWILQLDSADGKWQKDTQFLLITMTNSVGGFTNAVPDAAPDDGRLHVFLAPKLTLWRSLLAIPYFITGNF
QKLPGMTYFATKELTIKTPKTLQSRIDGDPSTKTPLTLKVISDHIQVLAPNVG
>Mature_293_residues
MLSYTIIYNPASGHSKGPTAAEQLKTKLEDRQHQVTMAPTKSADDAREFAYHAQADIVVAVGGDGTINQVVAGLAPRKNP
PLLAILPEGTVNNLAKVLHIPLLLPLAIKNILEAAPQPLDVAQVNNHYMVSTLTLGVLANAALSVTQKEKQHFGPIIYLM
KGFKVLAQHQHWILQLDSADGKWQKDTQFLLITMTNSVGGFTNAVPDAAPDDGRLHVFLAPKLTLWRSLLAIPYFITGNF
QKLPGMTYFATKELTIKTPKTLQSRIDGDPSTKTPLTLKVISDHIQVLAPNVG

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=257, Percent_Identity=24.124513618677, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 31871; Mature: 31871

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYTIIYNPASGHSKGPTAAEQLKTKLEDRQHQVTMAPTKSADDAREFAYHAQADIVVA
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHCCCCCEEEE
VGGDGTINQVVAGLAPRKNPPLLAILPEGTVNNLAKVLHIPLLLPLAIKNILEAAPQPLD
ECCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VAQVNNHYMVSTLTLGVLANAALSVTQKEKQHFGPIIYLMKGFKVLAQHQHWILQLDSAD
EEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCC
GKWQKDTQFLLITMTNSVGGFTNAVPDAAPDDGRLHVFLAPKLTLWRSLLAIPYFITGNF
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCEEEECCH
QKLPGMTYFATKELTIKTPKTLQSRIDGDPSTKTPLTLKVISDHIQVLAPNVG
HHCCCEEEEEECEEEEECCHHHHHHCCCCCCCCCCEEEEEECCCHHEECCCCC
>Mature Secondary Structure
MLSYTIIYNPASGHSKGPTAAEQLKTKLEDRQHQVTMAPTKSADDAREFAYHAQADIVVA
CEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHCCCCCEEEE
VGGDGTINQVVAGLAPRKNPPLLAILPEGTVNNLAKVLHIPLLLPLAIKNILEAAPQPLD
ECCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VAQVNNHYMVSTLTLGVLANAALSVTQKEKQHFGPIIYLMKGFKVLAQHQHWILQLDSAD
EEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCC
GKWQKDTQFLLITMTNSVGGFTNAVPDAAPDDGRLHVFLAPKLTLWRSLLAIPYFITGNF
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCEEEECCH
QKLPGMTYFATKELTIKTPKTLQSRIDGDPSTKTPLTLKVISDHIQVLAPNVG
HHCCCEEEEEECEEEEECCHHHHHHCCCCCCCCCCEEEEEECCCHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA