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The map label for this gene is ponA [H]

Identifier: 116494966

GI number: 116494966

Start: 1473527

End: 1475833

Strand: Direct

Name: ponA [H]

Synonym: LSEI_1481

Alternate gene names: 116494966

Gene position: 1473527-1475833 (Clockwise)

Preceding gene: 116494965

Following gene: 116494975

Centisome position: 50.89

GC content: 47.12

Gene sequence:

>2307_bases
GTGGCTGATAATCAAAATATGTCGCGAATGGCCCGACGCCGCGAAGATAAGTTCACGAAAGGATCTGCCCCTAAACCGCC
AAAACGATTATGGCGGCGAATTCTTAAGTGGACGTTGCTCGCGATCCTGGCATTTTTCATTCTCGGCGTTGGCCTGTTTT
CCTGGTACGCTAAAGACGCGCCAGAAGTGACTCAGGCAAAATTAGAGTCAGGCGGTTCCAGTACCATTTATGATCGATCT
GGCAATGAAATTACGACACTTGGTTTGGAAAGTCGGGATTATGTGAAGGCCTCGGAAATCCCGCAACAATTAAAAGATGC
CGTCGTCTCCATTGAGGACCGGCGCTTTTATGATGAGAAATTAGGGGTTGATCCTGTCCGAATCATCGGGGCAGCGTTTA
ATAATATAACAGGCACTAGCGATGGTTTACAGGGCGGCTCGACTTTAACGCAACAACTGATCAAATTGTCGGTTTTCAGT
ACCAAATCCTCCGACCAAACCCTTCGGCGTAAAGCACAAGAAGCTTGGTTGGCCATGCAGGTCCAACAAAAATATAGCAA
GGATCAGATTTTAGAGTATTACATTAACAAAGTTTTCATGAACTATGGTCAATATGGCATGAGCACGGGTGCTAAGTTTT
ACTTTAACAAGTCACTGAAGGATTTAACGCTTGCGCAGACTGCGTTTATTGCAGGCCTGCCGCAAAGCCCAGCCGGTTAT
GATCCCTATGAATATCCGCAAAAAGCAACTCAACGGCGCAATGCCGTCATTGATGCCATGTTGCGTGATAAAAAGATCAC
CGCGACGGCGGCTAAACAAGCCAAAGCAACACCAATCACTGATGGCTTAAAGCCAAAACAGCAGCAAACTAATACCGCAA
CCAATGACAAAGTGATTGATTCTTACCTCACTCAAGTGATTGCCGAAGTTAAGAAAAAGACCGGCCTTAATCCATATACC
GACAACTTGGATATTTATACTAATATCGATATGGGCGCCCAGCGTCGGCTTTATGACATTGTTAACACTGACGAATACGT
TAACTTCCCTGATGATGCCTTCCAGACCGGTGTCACAATGACCGATCCAAACAATGGTCAGGTTCTCGCACAAATCGGCG
GTCGAAAAACTGGCGACGTCCGATTGGCCTATAATCGTGCTGCACAAAATACACGTAGCAATGGGTCTACCATGAAACCG
TTGATGGATTATGGACCAGCAATCGAATATCTGAATTACTCCACTTACGAGCAGATGACCGATGAACCGTACACGTATCC
GGGCACGAGCATTTCACTTTATGATTGGGACAAGAAGTACCAAGGACGCATCTCTATGCGGACAGCACTTGAACAATCAC
GAAATATTCCAGCGGTCAAGACCCTTAGTACTGTTGGCATGGCGAATGCGGTTAAATTCCTCAGTGGTTTAGGGATCAAT
TTGCCAAGTTCGGAACAATATCTCTCAAGTGCGATCGGTGCTTCTGTCAATACCGTTCAAGAAGCTGGTGCTTATGGCGC
CTTTGCCAACGGCGGCACGTACTATAAACCGTACTATGTTAGCAAAGTTGTCACCGCTGATGGCAATACCCAAACCTTCA
ATAGTCAAGGCAGCCGGGCTATGAAGTCCAGTACCGCCTATATGATTACTGACATGTTAAAAGGCGTTCTGACCAAAGGA
ACCGGGACCTCGGCGGCTATTTCTGGACTGTATCAAGCTGGTAAAACTGGGACGACTGACTATAGTGATGCCGAACTCCA
ACAAAACCCCGCATTGAACGCAACTGGCATTGCCAAAGATGCCTGGTTCACAGGTTATACACGTAGCCGAGTCATTTCGG
TTTGGACCGGTTATGATAAACCGACTTCACACGGGATTAACTATGCTGAACAGACCATTTCACAAGAAATATACAAAGCA
CTAATGACTTATACGTCACAGAGTCTGACCAATCGTGATTGGACCAAGCCTGATACAGTTGAGTCTTATAATATTCTCAA
GGGAAGTAATCCAGGAACGGCCATTACCGGCGGATCTGGTGATACGACTAAAGAACTCTACGTCCGCGGACACGGCCCAA
GCAGCCGCAAAGCGGCAGTCGAGTCCTCTTCCTCGAGCTCAAGCAGTTCTGTTAGTTCCTCTTCACGAGAAAGTATTGAA
AGCTCAAGTTCAGTTTCCAGCTCTGAGCCGGTAGTATCAGCCTCTGCCTCCTCTCAACCTTCAAATCAAGAACCAAGTGC
CAGTTCAAGCGAACCAAGCACGCCTTCGGGTTCTGGTAGCGGCAGCGGTGGTAGTAACAACCCCTGA

Upstream 100 bases:

>100_bases
ATTGTCACAAACGGATTCGAGCTTCATCCACAACTACAGCCGGTCATCCCCTTTCTTGATGGGGTTGACTGGTTGATTGA
AACGAAAGTAGGTAATGTGC

Downstream 100 bases:

>100_bases
TAACGACTAAGCTGCTTCAGTAAACCCTAAAAAATAGCCTCATCCGCTTCTTGCACCTGCCTAACGACGGCAATGCAAGA
AGCGGATGAGGCTTATTTTT

Product: membrane carboxypeptidase (penicillin-binding protein)

Products: NA

Alternate protein names: PBP-1A [H]

Number of amino acids: Translated: 768; Mature: 767

Protein sequence:

>768_residues
MADNQNMSRMARRREDKFTKGSAPKPPKRLWRRILKWTLLAILAFFILGVGLFSWYAKDAPEVTQAKLESGGSSTIYDRS
GNEITTLGLESRDYVKASEIPQQLKDAVVSIEDRRFYDEKLGVDPVRIIGAAFNNITGTSDGLQGGSTLTQQLIKLSVFS
TKSSDQTLRRKAQEAWLAMQVQQKYSKDQILEYYINKVFMNYGQYGMSTGAKFYFNKSLKDLTLAQTAFIAGLPQSPAGY
DPYEYPQKATQRRNAVIDAMLRDKKITATAAKQAKATPITDGLKPKQQQTNTATNDKVIDSYLTQVIAEVKKKTGLNPYT
DNLDIYTNIDMGAQRRLYDIVNTDEYVNFPDDAFQTGVTMTDPNNGQVLAQIGGRKTGDVRLAYNRAAQNTRSNGSTMKP
LMDYGPAIEYLNYSTYEQMTDEPYTYPGTSISLYDWDKKYQGRISMRTALEQSRNIPAVKTLSTVGMANAVKFLSGLGIN
LPSSEQYLSSAIGASVNTVQEAGAYGAFANGGTYYKPYYVSKVVTADGNTQTFNSQGSRAMKSSTAYMITDMLKGVLTKG
TGTSAAISGLYQAGKTGTTDYSDAELQQNPALNATGIAKDAWFTGYTRSRVISVWTGYDKPTSHGINYAEQTISQEIYKA
LMTYTSQSLTNRDWTKPDTVESYNILKGSNPGTAITGGSGDTTKELYVRGHGPSSRKAAVESSSSSSSSSVSSSSRESIE
SSSSVSSSEPVVSASASSQPSNQEPSASSSEPSTPSGSGSGSGGSNNP

Sequences:

>Translated_768_residues
MADNQNMSRMARRREDKFTKGSAPKPPKRLWRRILKWTLLAILAFFILGVGLFSWYAKDAPEVTQAKLESGGSSTIYDRS
GNEITTLGLESRDYVKASEIPQQLKDAVVSIEDRRFYDEKLGVDPVRIIGAAFNNITGTSDGLQGGSTLTQQLIKLSVFS
TKSSDQTLRRKAQEAWLAMQVQQKYSKDQILEYYINKVFMNYGQYGMSTGAKFYFNKSLKDLTLAQTAFIAGLPQSPAGY
DPYEYPQKATQRRNAVIDAMLRDKKITATAAKQAKATPITDGLKPKQQQTNTATNDKVIDSYLTQVIAEVKKKTGLNPYT
DNLDIYTNIDMGAQRRLYDIVNTDEYVNFPDDAFQTGVTMTDPNNGQVLAQIGGRKTGDVRLAYNRAAQNTRSNGSTMKP
LMDYGPAIEYLNYSTYEQMTDEPYTYPGTSISLYDWDKKYQGRISMRTALEQSRNIPAVKTLSTVGMANAVKFLSGLGIN
LPSSEQYLSSAIGASVNTVQEAGAYGAFANGGTYYKPYYVSKVVTADGNTQTFNSQGSRAMKSSTAYMITDMLKGVLTKG
TGTSAAISGLYQAGKTGTTDYSDAELQQNPALNATGIAKDAWFTGYTRSRVISVWTGYDKPTSHGINYAEQTISQEIYKA
LMTYTSQSLTNRDWTKPDTVESYNILKGSNPGTAITGGSGDTTKELYVRGHGPSSRKAAVESSSSSSSSSVSSSSRESIE
SSSSVSSSEPVVSASASSQPSNQEPSASSSEPSTPSGSGSGSGGSNNP
>Mature_767_residues
ADNQNMSRMARRREDKFTKGSAPKPPKRLWRRILKWTLLAILAFFILGVGLFSWYAKDAPEVTQAKLESGGSSTIYDRSG
NEITTLGLESRDYVKASEIPQQLKDAVVSIEDRRFYDEKLGVDPVRIIGAAFNNITGTSDGLQGGSTLTQQLIKLSVFST
KSSDQTLRRKAQEAWLAMQVQQKYSKDQILEYYINKVFMNYGQYGMSTGAKFYFNKSLKDLTLAQTAFIAGLPQSPAGYD
PYEYPQKATQRRNAVIDAMLRDKKITATAAKQAKATPITDGLKPKQQQTNTATNDKVIDSYLTQVIAEVKKKTGLNPYTD
NLDIYTNIDMGAQRRLYDIVNTDEYVNFPDDAFQTGVTMTDPNNGQVLAQIGGRKTGDVRLAYNRAAQNTRSNGSTMKPL
MDYGPAIEYLNYSTYEQMTDEPYTYPGTSISLYDWDKKYQGRISMRTALEQSRNIPAVKTLSTVGMANAVKFLSGLGINL
PSSEQYLSSAIGASVNTVQEAGAYGAFANGGTYYKPYYVSKVVTADGNTQTFNSQGSRAMKSSTAYMITDMLKGVLTKGT
GTSAAISGLYQAGKTGTTDYSDAELQQNPALNATGIAKDAWFTGYTRSRVISVWTGYDKPTSHGINYAEQTISQEIYKAL
MTYTSQSLTNRDWTKPDTVESYNILKGSNPGTAITGGSGDTTKELYVRGHGPSSRKAAVESSSSSSSSSVSSSSRESIES
SSSVSSSEPVVSASASSQPSNQEPSASSSEPSTPSGSGSGSGGSNNP

Specific function: Cell wall formation [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=564, Percent_Identity=27.6595744680851, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI87082258, Length=309, Percent_Identity=32.0388349514563, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI1788867, Length=521, Percent_Identity=26.295585412668, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 83509; Mature: 83377

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNQNMSRMARRREDKFTKGSAPKPPKRLWRRILKWTLLAILAFFILGVGLFSWYAKDA
CCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEVTQAKLESGGSSTIYDRSGNEITTLGLESRDYVKASEIPQQLKDAVVSIEDRRFYDEK
CHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGVDPVRIIGAAFNNITGTSDGLQGGSTLTQQLIKLSVFSTKSSDQTLRRKAQEAWLAMQ
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VQQKYSKDQILEYYINKVFMNYGQYGMSTGAKFYFNKSLKDLTLAQTAFIAGLPQSPAGY
HHHHCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCC
DPYEYPQKATQRRNAVIDAMLRDKKITATAAKQAKATPITDGLKPKQQQTNTATNDKVID
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCHHHHH
SYLTQVIAEVKKKTGLNPYTDNLDIYTNIDMGAQRRLYDIVNTDEYVNFPDDAFQTGVTM
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHCCCEE
TDPNNGQVLAQIGGRKTGDVRLAYNRAAQNTRSNGSTMKPLMDYGPAIEYLNYSTYEQMT
ECCCCCEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCCHHHHCCCHHHHCCCHHHHHHC
DEPYTYPGTSISLYDWDKKYQGRISMRTALEQSRNIPAVKTLSTVGMANAVKFLSGLGIN
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
LPSSEQYLSSAIGASVNTVQEAGAYGAFANGGTYYKPYYVSKVVTADGNTQTFNSQGSRA
CCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEECCEEEEEEEECCCCCCCCCCCCCHH
MKSSTAYMITDMLKGVLTKGTGTSAAISGLYQAGKTGTTDYSDAELQQNPALNATGIAKD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCC
AWFTGYTRSRVISVWTGYDKPTSHGINYAEQTISQEIYKALMTYTSQSLTNRDWTKPDTV
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ESYNILKGSNPGTAITGGSGDTTKELYVRGHGPSSRKAAVESSSSSSSSSVSSSSRESIE
CCCCEEECCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
SSSSVSSSEPVVSASASSQPSNQEPSASSSEPSTPSGSGSGSGGSNNP
HHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADNQNMSRMARRREDKFTKGSAPKPPKRLWRRILKWTLLAILAFFILGVGLFSWYAKDA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEVTQAKLESGGSSTIYDRSGNEITTLGLESRDYVKASEIPQQLKDAVVSIEDRRFYDEK
CHHHHHHHHCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LGVDPVRIIGAAFNNITGTSDGLQGGSTLTQQLIKLSVFSTKSSDQTLRRKAQEAWLAMQ
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
VQQKYSKDQILEYYINKVFMNYGQYGMSTGAKFYFNKSLKDLTLAQTAFIAGLPQSPAGY
HHHHCCHHHHHHHHHHHHHHHHCHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCC
DPYEYPQKATQRRNAVIDAMLRDKKITATAAKQAKATPITDGLKPKQQQTNTATNDKVID
CCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCHHHHH
SYLTQVIAEVKKKTGLNPYTDNLDIYTNIDMGAQRRLYDIVNTDEYVNFPDDAFQTGVTM
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHCCCEE
TDPNNGQVLAQIGGRKTGDVRLAYNRAAQNTRSNGSTMKPLMDYGPAIEYLNYSTYEQMT
ECCCCCEEEEEECCCCCCCEEEEEHHHHHCCCCCCCCCCHHHHCCCHHHHCCCHHHHHHC
DEPYTYPGTSISLYDWDKKYQGRISMRTALEQSRNIPAVKTLSTVGMANAVKFLSGLGIN
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
LPSSEQYLSSAIGASVNTVQEAGAYGAFANGGTYYKPYYVSKVVTADGNTQTFNSQGSRA
CCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEECCEEEEEEEECCCCCCCCCCCCCHH
MKSSTAYMITDMLKGVLTKGTGTSAAISGLYQAGKTGTTDYSDAELQQNPALNATGIAKD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCC
AWFTGYTRSRVISVWTGYDKPTSHGINYAEQTISQEIYKALMTYTSQSLTNRDWTKPDTV
CCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ESYNILKGSNPGTAITGGSGDTTKELYVRGHGPSSRKAAVESSSSSSSSSVSSSSRESIE
CCCCEEECCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHCCCCCCCCHHHHHHHHHHHH
SSSSVSSSEPVVSASASSQPSNQEPSASSSEPSTPSGSGSGSGGSNNP
HHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1396576; 11463916 [H]