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The map label for this gene is recU

Identifier: 116494965

GI number: 116494965

Start: 1472908

End: 1473534

Strand: Direct

Name: recU

Synonym: LSEI_1480

Alternate gene names: 116494965

Gene position: 1472908-1473534 (Clockwise)

Preceding gene: 116494959

Following gene: 116494966

Centisome position: 50.87

GC content: 44.34

Gene sequence:

>627_bases
ATGACCATTCATTACCCTAATGGTAATCCATACAAAGACGGGACGCAATTTTCATCACAAGCTATTTCGCGTCCAACTAT
CTATGGTGGACGCGGTATGACGTTGGAAGAAGAATTGAACATCAGTAATCAATATTACCGCAGTATTGATAAAGCGGTCG
TCTACAAGAAACCGACCCCAGTTCAGATTGTGAAAGTTGACTACCCTAAGCGAAGTCAGGCTGTGATTCGCGAGGCCTAT
TTTAAAACGCCGTCAACAACAGATTATAATGGTGTTTATCGTGGATTTTACCTTGACTTTGAAGCCAAGGAAACTAAAAA
TAAAGCGAGCTTCCCGCTAAAAAACTTTCATCAGCATCAGATTGAGCATTTTCGGCGCTGTTTGAAACAAAGCGGGATCT
GTTTTGTGGTCATTCGGTTTGCAACCCTGAAACGTCTCTTTGTTTTCCCTGCCGGTCGACTGATTGACTGTTGGGACCGA
CAACCAGACGGCGGTCGCAAATCGATTCCATTGAAGGATATTGTCACAAACGGATTCGAGCTTCATCCACAACTACAGCC
GGTCATCCCCTTTCTTGATGGGGTTGACTGGTTGATTGAAACGAAAGTAGGTAATGTGCGTGGCTGA

Upstream 100 bases:

>100_bases
GGTACCCCGTGACCCATAATCGTTTTCCAACCATGACTTACCTCCGATTATTAGGCCAAAGCGGCTGATATTGGTATAAT
AGCGCAAGGAGTGTGATCGC

Downstream 100 bases:

>100_bases
TAATCAAAATATGTCGCGAATGGCCCGACGCCGCGAAGATAAGTTCACGAAAGGATCTGCCCCTAAACCGCCAAAACGAT
TATGGCGGCGAATTCTTAAG

Product: Holliday junction-specific endonuclease

Products: NA

Alternate protein names: Recombination protein U homolog

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTIHYPNGNPYKDGTQFSSQAISRPTIYGGRGMTLEEELNISNQYYRSIDKAVVYKKPTPVQIVKVDYPKRSQAVIREAY
FKTPSTTDYNGVYRGFYLDFEAKETKNKASFPLKNFHQHQIEHFRRCLKQSGICFVVIRFATLKRLFVFPAGRLIDCWDR
QPDGGRKSIPLKDIVTNGFELHPQLQPVIPFLDGVDWLIETKVGNVRG

Sequences:

>Translated_208_residues
MTIHYPNGNPYKDGTQFSSQAISRPTIYGGRGMTLEEELNISNQYYRSIDKAVVYKKPTPVQIVKVDYPKRSQAVIREAY
FKTPSTTDYNGVYRGFYLDFEAKETKNKASFPLKNFHQHQIEHFRRCLKQSGICFVVIRFATLKRLFVFPAGRLIDCWDR
QPDGGRKSIPLKDIVTNGFELHPQLQPVIPFLDGVDWLIETKVGNVRG
>Mature_207_residues
TIHYPNGNPYKDGTQFSSQAISRPTIYGGRGMTLEEELNISNQYYRSIDKAVVYKKPTPVQIVKVDYPKRSQAVIREAYF
KTPSTTDYNGVYRGFYLDFEAKETKNKASFPLKNFHQHQIEHFRRCLKQSGICFVVIRFATLKRLFVFPAGRLIDCWDRQ
PDGGRKSIPLKDIVTNGFELHPQLQPVIPFLDGVDWLIETKVGNVRG

Specific function: Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair

COG id: COG3331

COG function: function code R; Penicillin-binding protein-related factor A, putative recombinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recU family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECU_LACC3 (Q038W5)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806699.1
- ProteinModelPortal:   Q038W5
- SMR:   Q038W5
- STRING:   Q038W5
- GeneID:   4419856
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1480
- eggNOG:   COG3331
- HOGENOM:   HBG571967
- OMA:   GHYIDFE
- ProtClustDB:   PRK02234
- BioCyc:   LCAS321967:LSEI_1480-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00130
- InterPro:   IPR004612
- InterPro:   IPR011335
- PIRSF:   PIRSF037785
- TIGRFAMs:   TIGR00648

Pfam domain/function: PF03838 RecU; SSF52980 Restrict_endonuc_II-like_core

EC number: NA

Molecular weight: Translated: 23982; Mature: 23851

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHYPNGNPYKDGTQFSSQAISRPTIYGGRGMTLEEELNISNQYYRSIDKAVVYKKPTP
CEEECCCCCCCCCCCHHHHHHCCCCEEECCCCCCCHHHCCCCHHHHHHHHHHEEEECCCC
VQIVKVDYPKRSQAVIREAYFKTPSTTDYNGVYRGFYLDFEAKETKNKASFPLKNFHQHQ
EEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECHHCCCCCCCCHHHHHHHH
IEHFRRCLKQSGICFVVIRFATLKRLFVFPAGRLIDCWDRQPDGGRKSIPLKDIVTNGFE
HHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHCCCEE
LHPQLQPVIPFLDGVDWLIETKVGNVRG
ECCCCCHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TIHYPNGNPYKDGTQFSSQAISRPTIYGGRGMTLEEELNISNQYYRSIDKAVVYKKPTP
EEECCCCCCCCCCCHHHHHHCCCCEEECCCCCCCHHHCCCCHHHHHHHHHHEEEECCCC
VQIVKVDYPKRSQAVIREAYFKTPSTTDYNGVYRGFYLDFEAKETKNKASFPLKNFHQHQ
EEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECHHCCCCCCCCHHHHHHHH
IEHFRRCLKQSGICFVVIRFATLKRLFVFPAGRLIDCWDRQPDGGRKSIPLKDIVTNGFE
HHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHCCCEE
LHPQLQPVIPFLDGVDWLIETKVGNVRG
ECCCCCHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA