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The map label for this gene is trmFO

Identifier: 116494888

GI number: 116494888

Start: 1389571

End: 1390890

Strand: Direct

Name: trmFO

Synonym: LSEI_1402

Alternate gene names: 116494888

Gene position: 1389571-1390890 (Clockwise)

Preceding gene: 116494887

Following gene: 116494889

Centisome position: 47.99

GC content: 49.09

Gene sequence:

>1320_bases
ATGCCAACAGTTAATGTCATTGGTGCCGGACTAGCCGGATCAGAAGCAGCCTGGCAAATCGCTCAGGCCGGGGTAGATGT
CAACTTATACGAGATGCGTCCCGTTAAAATGACGCCTGCTCATCACACGAGCAATTTTGCTGAGCTTGTCTGCACGAATT
CACTGCGCGCCAACCAGATCACCAATGCGGTGGGTCTATTAAAGGAAGAAATGCGTCAGTTACATTCAATCATTATTCAA
AGTGCTGACGCCACCGCTGTCCCTGCTGGCGGTGCCTTGGCGGTGGATCGCGAACCATTTAGCGAGCTAGTGACCCAAAA
GTTGACGAGCAATTCGCATATTCATGTGGTGAATGAGGAATTGTCAGCATTTCCTGATGGGATCACAGTGGTGGCTACCG
GTCCGCTAACAGCGCCCGGGTTAGCACAGGCAATTGTTGATCTGAACGGCGAGGCCGGTTTGTCATTCTTTGACGCAGCC
GCACCGATTCTTGATGCGAATACGATCAACGAGGACATTGTTTATAAAAAGTCGCGCTATGATCGTGGCGAAGCCGCCTA
TTTGAATTGTCCGATGACGAAGGATGAATTCTTGGCGTTCTATCAAGCGCTCGTTTCTGCCGAAGTTGCTGAAGGCCATG
ATTTTGAAAAGATGACGGTTTTTGAAGGCTGCATGCCGATTGAAGTCATGGCCAAACGCGGTATTAAAACTATGTTGTTT
GGCCCGCTAAAGCCAGTTGGCCTTGAAGATCCACGGACAGGTAAGGAACCTTACGCGGTTGTCCAGTTGCGTCAAGATAA
CGCTGCAGCGAGTTTATATAACATGGTCGGGTTCCAAACTCATCTTAAATGGGGCGAGCAAAAACGGGTTTTCCGACTAA
TCCCTGGACTTGAAAACGTGCAAATCGTCCGGTATGGCGTCATGCATCGCAACACTTTCATGAAATCCCCAGTGGTTTTG
GAACCAACATATGCATCCAAACGCCGGCCTGATCTGTTCTTTGCAGGCCAGATGACTGGGGTGGAAGGTTATGTTGAAAG
CGCCGCCAGCGGTCTGATTGCGGGTATCAATGCGACCCGTATGGCGCTTGGAGAAACCCCGGTGATTTTTCCCGAAACTA
CAGCGATGGGGTCAATGGCGCATTACATTACTCACACATCCGCCCATCACTTCCAGCCGATGAATGCGAACTTTGGCATC
ATGCCAGCGTTAACAGTCAAAATACGCAATAAAAAAGAACGCAATCAGCAGTTAGCTGATCGTGCATTAAAAGATTTAGC
TACTTTCCAAGCAGGTATGAATCGGGTTGAACAAGTTTAA

Upstream 100 bases:

>100_bases
CGCTACCGCATCAGCAAGCCACCAAAATACACGTGCCGCAAAACTCACATTTTCTCTGAACGAACTAATTGCGCCACGCC
ACCACAAGGAGGAATCACTC

Downstream 100 bases:

>100_bases
GGACGCAAAAAATCTCGGGTTACCGGGATTTTTTCATTGGTATTTCGGCGGCTTGATGGTGTTGATGCACTGAAAGCGTC
AACAATGGCTGCATTTTATG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MPTVNVIGAGLAGSEAAWQIAQAGVDVNLYEMRPVKMTPAHHTSNFAELVCTNSLRANQITNAVGLLKEEMRQLHSIIIQ
SADATAVPAGGALAVDREPFSELVTQKLTSNSHIHVVNEELSAFPDGITVVATGPLTAPGLAQAIVDLNGEAGLSFFDAA
APILDANTINEDIVYKKSRYDRGEAAYLNCPMTKDEFLAFYQALVSAEVAEGHDFEKMTVFEGCMPIEVMAKRGIKTMLF
GPLKPVGLEDPRTGKEPYAVVQLRQDNAAASLYNMVGFQTHLKWGEQKRVFRLIPGLENVQIVRYGVMHRNTFMKSPVVL
EPTYASKRRPDLFFAGQMTGVEGYVESAASGLIAGINATRMALGETPVIFPETTAMGSMAHYITHTSAHHFQPMNANFGI
MPALTVKIRNKKERNQQLADRALKDLATFQAGMNRVEQV

Sequences:

>Translated_439_residues
MPTVNVIGAGLAGSEAAWQIAQAGVDVNLYEMRPVKMTPAHHTSNFAELVCTNSLRANQITNAVGLLKEEMRQLHSIIIQ
SADATAVPAGGALAVDREPFSELVTQKLTSNSHIHVVNEELSAFPDGITVVATGPLTAPGLAQAIVDLNGEAGLSFFDAA
APILDANTINEDIVYKKSRYDRGEAAYLNCPMTKDEFLAFYQALVSAEVAEGHDFEKMTVFEGCMPIEVMAKRGIKTMLF
GPLKPVGLEDPRTGKEPYAVVQLRQDNAAASLYNMVGFQTHLKWGEQKRVFRLIPGLENVQIVRYGVMHRNTFMKSPVVL
EPTYASKRRPDLFFAGQMTGVEGYVESAASGLIAGINATRMALGETPVIFPETTAMGSMAHYITHTSAHHFQPMNANFGI
MPALTVKIRNKKERNQQLADRALKDLATFQAGMNRVEQV
>Mature_438_residues
PTVNVIGAGLAGSEAAWQIAQAGVDVNLYEMRPVKMTPAHHTSNFAELVCTNSLRANQITNAVGLLKEEMRQLHSIIIQS
ADATAVPAGGALAVDREPFSELVTQKLTSNSHIHVVNEELSAFPDGITVVATGPLTAPGLAQAIVDLNGEAGLSFFDAAA
PILDANTINEDIVYKKSRYDRGEAAYLNCPMTKDEFLAFYQALVSAEVAEGHDFEKMTVFEGCMPIEVMAKRGIKTMLFG
PLKPVGLEDPRTGKEPYAVVQLRQDNAAASLYNMVGFQTHLKWGEQKRVFRLIPGLENVQIVRYGVMHRNTFMKSPVVLE
PTYASKRRPDLFFAGQMTGVEGYVESAASGLIAGINATRMALGETPVIFPETTAMGSMAHYITHTSAHHFQPMNANFGIM
PALTVKIRNKKERNQQLADRALKDLATFQAGMNRVEQV

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=129, Percent_Identity=35.6589147286822, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_LACC3 (Q039E2)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806622.1
- ProteinModelPortal:   Q039E2
- SMR:   Q039E2
- STRING:   Q039E2
- GeneID:   4419743
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1402
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   LCAS321967:LSEI_1402-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 47851; Mature: 47719

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTVNVIGAGLAGSEAAWQIAQAGVDVNLYEMRPVKMTPAHHTSNFAELVCTNSLRANQI
CCCCEEEECCCCCCHHHHHHHHCCCCEEEEEECCEEECCCCCCCCHHHHEECCCCHHHHH
TNAVGLLKEEMRQLHSIIIQSADATAVPAGGALAVDREPFSELVTQKLTSNSHIHVVNEE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCH
LSAFPDGITVVATGPLTAPGLAQAIVDLNGEAGLSFFDAAAPILDANTINEDIVYKKSRY
HHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHCCEECCCCCCHHHEEECCCC
DRGEAAYLNCPMTKDEFLAFYQALVSAEVAEGHDFEKMTVFEGCMPIEVMAKRGIKTMLF
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCHHHHHHCCCCEEEE
GPLKPVGLEDPRTGKEPYAVVQLRQDNAAASLYNMVGFQTHLKWGEQKRVFRLIPGLENV
CCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
QIVRYGVMHRNTFMKSPVVLEPTYASKRRPDLFFAGQMTGVEGYVESAASGLIAGINATR
EEEEEEHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHCCEEECCCHHH
MALGETPVIFPETTAMGSMAHYITHTSAHHFQPMNANFGIMPALTVKIRNKKERNQQLAD
HCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHH
RALKDLATFQAGMNRVEQV
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PTVNVIGAGLAGSEAAWQIAQAGVDVNLYEMRPVKMTPAHHTSNFAELVCTNSLRANQI
CCCEEEECCCCCCHHHHHHHHCCCCEEEEEECCEEECCCCCCCCHHHHEECCCCHHHHH
TNAVGLLKEEMRQLHSIIIQSADATAVPAGGALAVDREPFSELVTQKLTSNSHIHVVNEE
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCH
LSAFPDGITVVATGPLTAPGLAQAIVDLNGEAGLSFFDAAAPILDANTINEDIVYKKSRY
HHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHHCCEECCCCCCHHHEEECCCC
DRGEAAYLNCPMTKDEFLAFYQALVSAEVAEGHDFEKMTVFEGCMPIEVMAKRGIKTMLF
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHCCCCCHHHHHHCCCCEEEE
GPLKPVGLEDPRTGKEPYAVVQLRQDNAAASLYNMVGFQTHLKWGEQKRVFRLIPGLENV
CCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
QIVRYGVMHRNTFMKSPVVLEPTYASKRRPDLFFAGQMTGVEGYVESAASGLIAGINATR
EEEEEEHHHHHHHCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHCCEEECCCHHH
MALGETPVIFPETTAMGSMAHYITHTSAHHFQPMNANFGIMPALTVKIRNKKERNQQLAD
HCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHH
RALKDLATFQAGMNRVEQV
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA