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The map label for this gene is folA [H]

Identifier: 116494874

GI number: 116494874

Start: 1377411

End: 1377902

Strand: Direct

Name: folA [H]

Synonym: LSEI_1388

Alternate gene names: 116494874

Gene position: 1377411-1377902 (Clockwise)

Preceding gene: 116494873

Following gene: 116494876

Centisome position: 47.57

GC content: 47.36

Gene sequence:

>492_bases
ATGGTAGCATTTTTGTGGGCGCAGGATCGGGATGGTGTAATCGGTAAAGACGGCCATTTGCCATGGCATTTGCCAGATGA
TTTGCATTATTTCCGGACTCAGACTGAAGGGAAAATGATGGTGGTTGGGCGTCGCACGTACGAAAGTTTTCCAAAACGGC
CATTACCAGATCGTACGAACGTGGTTTTGACGCACCAGGCTGATTACCAGGCACCAGGCGCGATTGTCTTGCATCAGGTT
GCTGAAGTGCTTGATTATGCGAAGGAACATGCAGATCAGGCATTAGTCATCGCCGGTGGTGCTCAAATCTTTAGCGCCTT
TAAAGACATGGTTGATACCTTGCTCGTGACCCGTCTAGCTGGCAGTTTTGCAGGTGACACTAAAATGATTCCACTAGATT
GGGATGCGTTTACTAAAACCTCAAGCCGAACTGTCGAAGATCAAAACCCCGCTTTGACGCATACTTATGAAGTTTGGCAA
AAGCAAAAATGA

Upstream 100 bases:

>100_bases
CGGCGAGAAAGCTTATTATTGGCGATATCGAAGCTGTTTAAGTGACGGTTCTGACTGATTGCAGTACCATCAGACGTATC
AAAAACGAGGGGGATTTTAA

Downstream 100 bases:

>100_bases
TCTGACGCGTTTAGCAGCTAAAGAAATTTTTTAAGGAACTTAAATAGTTATGTGCATTTGTAGTTCGTTTTTTTAACTAA
AATTGACTCATGTGCAAAAA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MVAFLWAQDRDGVIGKDGHLPWHLPDDLHYFRTQTEGKMMVVGRRTYESFPKRPLPDRTNVVLTHQADYQAPGAIVLHQV
AEVLDYAKEHADQALVIAGGAQIFSAFKDMVDTLLVTRLAGSFAGDTKMIPLDWDAFTKTSSRTVEDQNPALTHTYEVWQ
KQK

Sequences:

>Translated_163_residues
MVAFLWAQDRDGVIGKDGHLPWHLPDDLHYFRTQTEGKMMVVGRRTYESFPKRPLPDRTNVVLTHQADYQAPGAIVLHQV
AEVLDYAKEHADQALVIAGGAQIFSAFKDMVDTLLVTRLAGSFAGDTKMIPLDWDAFTKTSSRTVEDQNPALTHTYEVWQ
KQK
>Mature_163_residues
MVAFLWAQDRDGVIGKDGHLPWHLPDDLHYFRTQTEGKMMVVGRRTYESFPKRPLPDRTNVVLTHQADYQAPGAIVLHQV
AEVLDYAKEHADQALVIAGGAQIFSAFKDMVDTLLVTRLAGSFAGDTKMIPLDWDAFTKTSSRTVEDQNPALTHTYEVWQ
KQK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=141, Percent_Identity=30.4964539007092, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17505899, Length=162, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18411; Mature: 18411

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAFLWAQDRDGVIGKDGHLPWHLPDDLHYFRTQTEGKMMVVGRRTYESFPKRPLPDRTN
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCHHHHCCCCCCCCCCC
VVLTHQADYQAPGAIVLHQVAEVLDYAKEHADQALVIAGGAQIFSAFKDMVDTLLVTRLA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
GSFAGDTKMIPLDWDAFTKTSSRTVEDQNPALTHTYEVWQKQK
CCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MVAFLWAQDRDGVIGKDGHLPWHLPDDLHYFRTQTEGKMMVVGRRTYESFPKRPLPDRTN
CEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCHHHHCCCCCCCCCCC
VVLTHQADYQAPGAIVLHQVAEVLDYAKEHADQALVIAGGAQIFSAFKDMVDTLLVTRLA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
GSFAGDTKMIPLDWDAFTKTSSRTVEDQNPALTHTYEVWQKQK
CCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3928445; 98527; 405008; 6815179; 1905571; 7547901; 9514736; 10091649 [H]