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The map label for this gene is cca

Identifier: 116494870

GI number: 116494870

Start: 1372527

End: 1373723

Strand: Direct

Name: cca

Synonym: LSEI_1384

Alternate gene names: 116494870

Gene position: 1372527-1373723 (Clockwise)

Preceding gene: 116494868

Following gene: 116494871

Centisome position: 47.41

GC content: 50.13

Gene sequence:

>1197_bases
ATGCGACTAGATTTGACCCAGCCAGATTTTAAGGCAGCGATTCCGATTTTAAAAAAAATCGAAGCTGCTGGTTATGAAGC
TTATTTTGTTGGCGGCAGTGTTCGTGATGCGATACTGGGGCTGCCGATTCACGATGTAGATATTGCCAGTTCGGCGTATC
CGGCAGAAATTAAGCGAATTTTCAAGCGGACAGCCGATACCGGCATCGAGCATGGCACAGTGATGGTGCTTGATCACGGG
ACTGGCTATGAAGTGACAACTTTCCGAACCGAATCGCGCTATCAGGATTTTCGCCGGCCTGATCATGTGACTTTTGTGCG
CTCCTTAGCAGAAGATTTAAAGCGTCGGGATTTCACTATCAACGCTTTAGCTGTTCGGCATGATGGCACGATCATTGATT
TGTTTGACGGCCTGAAAGATTTGCAGCACCACCAGCTGCGGGCAGTGGGTAATCCTCATGAACGGTTTCATGAGGATGCT
TTGCGAATGATGCGGGCAGTTCGATTCGAGAGCCAATTGGGGTTCGGCATTGAGCCAGCCACCAAAGCGGCAATCGCCGA
CAATGCCAAACTGTTAGTGCACATTTCTGTAGAACGAGTCGCCGCAGAATTTAATCGGTTGCTGACTGGGATTGATCGAC
GTGCTGGCTTGCAAGATTTTATTGAGACTCGCTTGTTTGCCTACGCCCCGAAAATGGCGGCTCATGAAACAGCCTTAACG
CAATTTTCTCAGTTGCCTGATACGCAGCTGACCAGTTTGGCAAGCGGCTGGGCGGCATTGATTTTCCTTTTAAGAACACA
GCCGCTGGTGATGCTAAAGGCCTGGAAGCAATCCAATGAGCTGATCACCTTGGTCACGCAAACTGTCAAGCTACTGCAGG
TGATGCCGAATCCGACTGCTTGGGATCTTTACCAAGCTGGTGAAGCGGCCGTGACAACGGCTAGCGAGGTTCAAAAACTG
CTGACGCCTGACTTTGATCAGCAGCAGCTAGCTGAGGCTTACGCGGCCTTACCGATTCATAGTAAAAAAGAATTAGCGCT
GACCGGTGCAGATCTGATTAAAGCGGGGGTCCGTCCCGGGCCAGCGATGGGCAAAGCACTCAATCAAATCGAGCAGCGTG
TCGTCGCTGGAGCTTTGCCAAATGAACTAAAAAAGTTGTTGCCGATCGCAACTGAAATGTCTCAAGATCGTTTGTGA

Upstream 100 bases:

>100_bases
CCGCCTCCCAAAAAAGAATTTAAGCTTATTCTAACAGAAATGTCATATTTCGGCTTAATTGTGGTAATGGTGGTTGCCGG
TTTGGAAAAAGGAGTTTGAA

Downstream 100 bases:

>100_bases
GTGTGTTATGATGAGCATGTAAGCACATTCAACAACGTCAAACAGGACGATTGATCAGCTGGGCGGCTTTAAAATAGCAT
AGTTGGCCACCAGTGCGTTT

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MRLDLTQPDFKAAIPILKKIEAAGYEAYFVGGSVRDAILGLPIHDVDIASSAYPAEIKRIFKRTADTGIEHGTVMVLDHG
TGYEVTTFRTESRYQDFRRPDHVTFVRSLAEDLKRRDFTINALAVRHDGTIIDLFDGLKDLQHHQLRAVGNPHERFHEDA
LRMMRAVRFESQLGFGIEPATKAAIADNAKLLVHISVERVAAEFNRLLTGIDRRAGLQDFIETRLFAYAPKMAAHETALT
QFSQLPDTQLTSLASGWAALIFLLRTQPLVMLKAWKQSNELITLVTQTVKLLQVMPNPTAWDLYQAGEAAVTTASEVQKL
LTPDFDQQQLAEAYAALPIHSKKELALTGADLIKAGVRPGPAMGKALNQIEQRVVAGALPNELKKLLPIATEMSQDRL

Sequences:

>Translated_398_residues
MRLDLTQPDFKAAIPILKKIEAAGYEAYFVGGSVRDAILGLPIHDVDIASSAYPAEIKRIFKRTADTGIEHGTVMVLDHG
TGYEVTTFRTESRYQDFRRPDHVTFVRSLAEDLKRRDFTINALAVRHDGTIIDLFDGLKDLQHHQLRAVGNPHERFHEDA
LRMMRAVRFESQLGFGIEPATKAAIADNAKLLVHISVERVAAEFNRLLTGIDRRAGLQDFIETRLFAYAPKMAAHETALT
QFSQLPDTQLTSLASGWAALIFLLRTQPLVMLKAWKQSNELITLVTQTVKLLQVMPNPTAWDLYQAGEAAVTTASEVQKL
LTPDFDQQQLAEAYAALPIHSKKELALTGADLIKAGVRPGPAMGKALNQIEQRVVAGALPNELKKLLPIATEMSQDRL
>Mature_398_residues
MRLDLTQPDFKAAIPILKKIEAAGYEAYFVGGSVRDAILGLPIHDVDIASSAYPAEIKRIFKRTADTGIEHGTVMVLDHG
TGYEVTTFRTESRYQDFRRPDHVTFVRSLAEDLKRRDFTINALAVRHDGTIIDLFDGLKDLQHHQLRAVGNPHERFHEDA
LRMMRAVRFESQLGFGIEPATKAAIADNAKLLVHISVERVAAEFNRLLTGIDRRAGLQDFIETRLFAYAPKMAAHETALT
QFSQLPDTQLTSLASGWAALIFLLRTQPLVMLKAWKQSNELITLVTQTVKLLQVMPNPTAWDLYQAGEAAVTTASEVQKL
LTPDFDQQQLAEAYAALPIHSKKELALTGADLIKAGVRPGPAMGKALNQIEQRVVAGALPNELKKLLPIATEMSQDRL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=199, Percent_Identity=34.6733668341709, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI87081691, Length=207, Percent_Identity=36.7149758454106, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789436, Length=198, Percent_Identity=37.3737373737374, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71995920, Length=376, Percent_Identity=27.6595744680851, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321016, Length=209, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281360112, Length=249, Percent_Identity=32.9317269076305, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357337, Length=249, Percent_Identity=32.9317269076305, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_LACC3 (Q039G0)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806604.1
- ProteinModelPortal:   Q039G0
- SMR:   Q039G0
- STRING:   Q039G0
- GeneID:   4419803
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1384
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   KHDIVVN
- ProtClustDB:   CLSK770894
- BioCyc:   LCAS321967:LSEI_1384-MONOMER
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 44207; Mature: 44207

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 35-35 BINDING 116-116 BINDING 159-159 BINDING 162-162 BINDING 165-165 BINDING 168-168

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDLTQPDFKAAIPILKKIEAAGYEAYFVGGSVRDAILGLPIHDVDIASSAYPAEIKRI
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
FKRTADTGIEHGTVMVLDHGTGYEVTTFRTESRYQDFRRPDHVTFVRSLAEDLKRRDFTI
HHHHHHCCCCCCEEEEEECCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCEE
NALAVRHDGTIIDLFDGLKDLQHHQLRAVGNPHERFHEDALRMMRAVRFESQLGFGIEPA
EEEEEECCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TKAAIADNAKLLVHISVERVAAEFNRLLTGIDRRAGLQDFIETRLFAYAPKMAAHETALT
HHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHH
QFSQLPDTQLTSLASGWAALIFLLRTQPLVMLKAWKQSNELITLVTQTVKLLQVMPNPTA
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
WDLYQAGEAAVTTASEVQKLLTPDFDQQQLAEAYAALPIHSKKELALTGADLIKAGVRPG
CHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHEEECHHHHHHCCCCC
PAMGKALNQIEQRVVAGALPNELKKLLPIATEMSQDRL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLDLTQPDFKAAIPILKKIEAAGYEAYFVGGSVRDAILGLPIHDVDIASSAYPAEIKRI
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
FKRTADTGIEHGTVMVLDHGTGYEVTTFRTESRYQDFRRPDHVTFVRSLAEDLKRRDFTI
HHHHHHCCCCCCEEEEEECCCCCEEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCEE
NALAVRHDGTIIDLFDGLKDLQHHQLRAVGNPHERFHEDALRMMRAVRFESQLGFGIEPA
EEEEEECCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TKAAIADNAKLLVHISVERVAAEFNRLLTGIDRRAGLQDFIETRLFAYAPKMAAHETALT
HHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHH
QFSQLPDTQLTSLASGWAALIFLLRTQPLVMLKAWKQSNELITLVTQTVKLLQVMPNPTA
HHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
WDLYQAGEAAVTTASEVQKLLTPDFDQQQLAEAYAALPIHSKKELALTGADLIKAGVRPG
CHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHEEECHHHHHHCCCCC
PAMGKALNQIEQRVVAGALPNELKKLLPIATEMSQDRL
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA