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The map label for this gene is pfkA

Identifier: 116494850

GI number: 116494850

Start: 1353610

End: 1354569

Strand: Direct

Name: pfkA

Synonym: LSEI_1364

Alternate gene names: 116494850

Gene position: 1353610-1354569 (Clockwise)

Preceding gene: 116494849

Following gene: 116494851

Centisome position: 46.75

GC content: 50.21

Gene sequence:

>960_bases
ATGAAACGCATTGGTATTTTGACCAGTGGCGGGGATGCGCCTGGCATGAACGCCGCGGTCCGCGCTGTTGCTCGCAAGGC
GATGCACGAAGGCCTTGAAGTATATGGCATCAATTATGGATTTGCTGGGTTAGTTGCCGGCGACATTTTCAAAATGAATG
AATCAACTGTCGGTGATAAGATTCAACGCGGCGGGACCATGTTATATTCCGCACGTTATCCACAATTTGCGCAAGAAGAA
GGCCAGTTGCGCGGGGTCGAACAATTAAACAAGTTTGGCATCGAGGCGTTGGTCGTCATCGGCGGAGACGGCTCTTATCA
CGGTGCACTAGCTTTGACGCGTCACGGTTTCAACACCATTGGCCTGCCAGGAACGATCGACAACGATATTCCTTACACGG
ACTTCACGATTGGCTTTGACACTGCCGTTAATACAGTTGTGGAAGCTGTTGACCGTCTTCGCGATACCGCGGCTTCACAT
GAACGAACCTTTGTCATTGAAGTCATGGGTCGTGAAGCTGGGGATATTGCCCTTTGGTCGGGCGTCGCCGGTGGTGCCGA
GGATGTCATCATTCCAGAACACGACTTTGACGTTAAGAAAATTGCGTCGAAGTTGCAAAGTTCTCGTGAACGTGGTCAAA
AGCATGCGGTCATTCTTTTGGCAGAAGGCGTGATGCACGCTGATCAATTTGCCAAGGAATTAGCCGCACATGGCGATTTC
CAATTACGTTCGACAGTTCTGGGACACATTGTTCGTGGCGGTGCACCGTCCGCACGTGATCGCGTTTTGGCTTCACAAAT
GGGTTCATACGCGGTTGAACTTTTGTTGCAAGGCAAAGGCGCTTTGGCTGTCGGCATTGAAAATAACAAGATCACGGCAC
ATGATGTTCGGACCTTGTTTGATGCCAAGCATCACGCAGAACTTTCGCTCTACACCTTAGCGGAAGAGTTAACATTTTAA

Upstream 100 bases:

>100_bases
AAATGTTCTCATTTTCAGGGACTTTGTTTCTGAAAAGTGGTAAACTCAATGAGTATTTGTGATGGGACAAATCGCAAAAG
AATATGCATGAGGTGAAATA

Downstream 100 bases:

>100_bases
TCTCGTAAACAAACGACATTAACGTCTGTTTAAATAATTTCAAGGAGCGATTTCACTTATGAAAAAAACCAAGATCGTCA
GCACGCTTGGACCTGCAAGT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKRIGILTSGGDAPGMNAAVRAVARKAMHEGLEVYGINYGFAGLVAGDIFKMNESTVGDKIQRGGTMLYSARYPQFAQEE
GQLRGVEQLNKFGIEALVVIGGDGSYHGALALTRHGFNTIGLPGTIDNDIPYTDFTIGFDTAVNTVVEAVDRLRDTAASH
ERTFVIEVMGREAGDIALWSGVAGGAEDVIIPEHDFDVKKIASKLQSSRERGQKHAVILLAEGVMHADQFAKELAAHGDF
QLRSTVLGHIVRGGAPSARDRVLASQMGSYAVELLLQGKGALAVGIENNKITAHDVRTLFDAKHHAELSLYTLAEELTF

Sequences:

>Translated_319_residues
MKRIGILTSGGDAPGMNAAVRAVARKAMHEGLEVYGINYGFAGLVAGDIFKMNESTVGDKIQRGGTMLYSARYPQFAQEE
GQLRGVEQLNKFGIEALVVIGGDGSYHGALALTRHGFNTIGLPGTIDNDIPYTDFTIGFDTAVNTVVEAVDRLRDTAASH
ERTFVIEVMGREAGDIALWSGVAGGAEDVIIPEHDFDVKKIASKLQSSRERGQKHAVILLAEGVMHADQFAKELAAHGDF
QLRSTVLGHIVRGGAPSARDRVLASQMGSYAVELLLQGKGALAVGIENNKITAHDVRTLFDAKHHAELSLYTLAEELTF
>Mature_319_residues
MKRIGILTSGGDAPGMNAAVRAVARKAMHEGLEVYGINYGFAGLVAGDIFKMNESTVGDKIQRGGTMLYSARYPQFAQEE
GQLRGVEQLNKFGIEALVVIGGDGSYHGALALTRHGFNTIGLPGTIDNDIPYTDFTIGFDTAVNTVVEAVDRLRDTAASH
ERTFVIEVMGREAGDIALWSGVAGGAEDVIIPEHDFDVKKIASKLQSSRERGQKHAVILLAEGVMHADQFAKELAAHGDF
QLRSTVLGHIVRGGAPSARDRVLASQMGSYAVELLLQGKGALAVGIENNKITAHDVRTLFDAKHHAELSLYTLAEELTF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=42.1221864951768, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI11321601, Length=311, Percent_Identity=40.8360128617363, Blast_Score=202, Evalue=2e-52,
Organism=Homo sapiens, GI266453619, Length=314, Percent_Identity=41.0828025477707, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI266453768, Length=314, Percent_Identity=41.0828025477707, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI266453748, Length=314, Percent_Identity=41.0828025477707, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4505749, Length=314, Percent_Identity=41.0828025477707, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1790350, Length=296, Percent_Identity=52.027027027027, Blast_Score=313, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI25147584, Length=315, Percent_Identity=38.0952380952381, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17558788, Length=319, Percent_Identity=36.6771159874608, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI72003601, Length=312, Percent_Identity=29.4871794871795, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321679, Length=330, Percent_Identity=37.5757575757576, Blast_Score=182, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6323861, Length=330, Percent_Identity=34.8484848484849, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=40.1929260450161, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=40.1929260450161, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI28573326, Length=311, Percent_Identity=39.871382636656, Blast_Score=199, Evalue=2e-51,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_LACC3 (Q039I0)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806584.1
- ProteinModelPortal:   Q039I0
- SMR:   Q039I0
- STRING:   Q039I0
- GeneID:   4419087
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1364
- eggNOG:   COG0205
- HOGENOM:   HBG405044
- OMA:   VDAIENM
- PhylomeDB:   Q039I0
- ProtClustDB:   PRK03202
- BioCyc:   LCAS321967:LSEI_1364-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34230; Mature: 34230

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 243-243 BINDING 249-249 BINDING 252-252

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGILTSGGDAPGMNAAVRAVARKAMHEGLEVYGINYGFAGLVAGDIFKMNESTVGDK
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHEECCCCHHHHH
IQRGGTMLYSARYPQFAQEEGQLRGVEQLNKFGIEALVVIGGDGSYHGALALTRHGFNTI
HHCCCEEEEECCCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCC
GLPGTIDNDIPYTDFTIGFDTAVNTVVEAVDRLRDTAASHERTFVIEVMGREAGDIALWS
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEC
GVAGGAEDVIIPEHDFDVKKIASKLQSSRERGQKHAVILLAEGVMHADQFAKELAAHGDF
CCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCH
QLRSTVLGHIVRGGAPSARDRVLASQMGSYAVELLLQGKGALAVGIENNKITAHDVRTLF
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEHHHHHHHH
DAKHHAELSLYTLAEELTF
CCHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKRIGILTSGGDAPGMNAAVRAVARKAMHEGLEVYGINYGFAGLVAGDIFKMNESTVGDK
CCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHEECCCCHHHHH
IQRGGTMLYSARYPQFAQEEGQLRGVEQLNKFGIEALVVIGGDGSYHGALALTRHGFNTI
HHCCCEEEEECCCCCHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECCCCCC
GLPGTIDNDIPYTDFTIGFDTAVNTVVEAVDRLRDTAASHERTFVIEVMGREAGDIALWS
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEC
GVAGGAEDVIIPEHDFDVKKIASKLQSSRERGQKHAVILLAEGVMHADQFAKELAAHGDF
CCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHCCCH
QLRSTVLGHIVRGGAPSARDRVLASQMGSYAVELLLQGKGALAVGIENNKITAHDVRTLF
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEHHHHHHHH
DAKHHAELSLYTLAEELTF
CCHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA