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The map label for this gene is tuf [H]

Identifier: 116494821

GI number: 116494821

Start: 1316761

End: 1317951

Strand: Direct

Name: tuf [H]

Synonym: LSEI_1332

Alternate gene names: 116494821

Gene position: 1316761-1317951 (Clockwise)

Preceding gene: 116494820

Following gene: 116494823

Centisome position: 45.48

GC content: 47.77

Gene sequence:

>1191_bases
ATGGCTGAAAAAGAACACTATGAACGCACAAAGCCCCACGTAAACATTGGTACGATCGGGCACGTTGACCACGGTAAGAC
TACTTTGACCGCGGCTATCACTAAGGTATTGTCAGAAAAGGGCCTTGCCAAAGCGCAAGACTACGCTTCTATCGATGCTG
CTCCAGAAGAAAAGGAACGTGGCATCACAATCAACACCGCCCACGTTGAATATGAAACCGAAAAGCGCCACTACGCACAT
ATTGATGCGCCAGGGCATGCTGACTACGTTAAGAACATGATCACCGGTGCTGCACAGATGGATGGTGCGATCTTGGTTGT
TGCGGCAACTGATGGCCCAATGCCACAGACCCGTGAGCATATCTTGTTGGCTCGTCAGGTCGGCGTTGATTACATCGTTG
TTTTCCTGAACAAGACAGACTTGGTTGACGATCCAGAATTGATCGACTTGGTTGAAATGGAAGTCCGGGAACTGCTCAGC
GAATACGATTATCCTGGTGATGATATTCCTGTTATCCGTGGTTCTGCTTTGAAGGCCCTTGAAGGCGATCCAGAACAGGA
AAAGGTTATCATGGAATTGATGGATACCATCGATGAATATATCCCAACACCTGTTCGTGAAACAGACAAGCCTTTCTTGA
TGCCTGTTGAAGATGTCTTCACCATCACTGGTCGTGGTACAGTTGCTTCTGGCCGTATCGATCGTGGGACGGTTAAGATT
GGTGACGAAGTTGAAATCATCGGCTTGAAGCCAGATGTTATCAAGTCTACCGTTACTGGTCTTGAAATGTTCCGTAAGAC
CTTGGATCTTGGTGAAGCCGGCGATAACGTTGGTGTCTTGCTTCGTGGTGTTAACCGCGAACAAGTTGAACGTGGCCAAG
TTTTGGCAAAGCCAGGTTCAATCCAATTGCACAACAAGTTCAAGGGTGAAGTTTATATCTTGACAAAAGAAGAAGGTGGC
CGTCATACGCCATTCTTCTCAAACTATCGTCCGCAGTTCTACTTCCACACCACTGATGTTACCGGTGTGATTGAATTGCC
AGATGGCGTTGAAATGGTTATGCCTGGCGATAACGTTACTTTCGAAGTTGACCTGATTGCTCCGGTTGCTATCGAAAAAG
GTACCAAGTTCACCGTCCGTGAAGGCGGCCGTACTGTTGGTGCCGGCGTTGTTTCCGAAATCCTTGACTAA

Upstream 100 bases:

>100_bases
GGTATTTACGCTCAAATGTAGTACACTTAAACGTAAAGGATTATCAGGGTACACAAGGTATCCTGTATTCTTACGAAAAA
TACAGGAGGTTCGTTTTTTC

Downstream 100 bases:

>100_bases
TTTCTGAATATACGCATGAACCCGCAAGCTTGCTTGCGGGTTTTTTCGGCTCTACGTCAACTGGTGTTGAAGAATAAATT
TATCATTTGAGGCGGTTCTC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAEKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKAQDYASIDAAPEEKERGITINTAHVEYETEKRHYAH
IDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVDYIVVFLNKTDLVDDPELIDLVEMEVRELLS
EYDYPGDDIPVIRGSALKALEGDPEQEKVIMELMDTIDEYIPTPVRETDKPFLMPVEDVFTITGRGTVASGRIDRGTVKI
GDEVEIIGLKPDVIKSTVTGLEMFRKTLDLGEAGDNVGVLLRGVNREQVERGQVLAKPGSIQLHNKFKGEVYILTKEEGG
RHTPFFSNYRPQFYFHTTDVTGVIELPDGVEMVMPGDNVTFEVDLIAPVAIEKGTKFTVREGGRTVGAGVVSEILD

Sequences:

>Translated_396_residues
MAEKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKAQDYASIDAAPEEKERGITINTAHVEYETEKRHYAH
IDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVDYIVVFLNKTDLVDDPELIDLVEMEVRELLS
EYDYPGDDIPVIRGSALKALEGDPEQEKVIMELMDTIDEYIPTPVRETDKPFLMPVEDVFTITGRGTVASGRIDRGTVKI
GDEVEIIGLKPDVIKSTVTGLEMFRKTLDLGEAGDNVGVLLRGVNREQVERGQVLAKPGSIQLHNKFKGEVYILTKEEGG
RHTPFFSNYRPQFYFHTTDVTGVIELPDGVEMVMPGDNVTFEVDLIAPVAIEKGTKFTVREGGRTVGAGVVSEILD
>Mature_395_residues
AEKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKAQDYASIDAAPEEKERGITINTAHVEYETEKRHYAHI
DAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVDYIVVFLNKTDLVDDPELIDLVEMEVRELLSE
YDYPGDDIPVIRGSALKALEGDPEQEKVIMELMDTIDEYIPTPVRETDKPFLMPVEDVFTITGRGTVASGRIDRGTVKIG
DEVEIIGLKPDVIKSTVTGLEMFRKTLDLGEAGDNVGVLLRGVNREQVERGQVLAKPGSIQLHNKFKGEVYILTKEEGGR
HTPFFSNYRPQFYFHTTDVTGVIELPDGVEMVMPGDNVTFEVDLIAPVAIEKGTKFTVREGGRTVGAGVVSEILD

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=58.3756345177665, Blast_Score=478, Evalue=1e-135,
Organism=Homo sapiens, GI4503475, Length=452, Percent_Identity=28.9823008849558, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=29.3453724604966, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=29.3453724604966, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI4503471, Length=452, Percent_Identity=28.7610619469027, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI54607086, Length=314, Percent_Identity=29.9363057324841, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI46094014, Length=382, Percent_Identity=28.5340314136126, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI194018522, Length=375, Percent_Identity=28, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI194097354, Length=375, Percent_Identity=28, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI194018520, Length=375, Percent_Identity=28, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI4503507, Length=287, Percent_Identity=28.2229965156794, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI157426893, Length=242, Percent_Identity=29.7520661157025, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=34.0740740740741, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=72.0101781170484, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1789737, Length=392, Percent_Identity=72.1938775510204, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI2367247, Length=338, Percent_Identity=31.9526627218935, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994988, Length=280, Percent_Identity=34.6428571428571, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789108, Length=325, Percent_Identity=28.6153846153846, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=329, Percent_Identity=26.7477203647416, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=53.5353535353535, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=46.1340206185567, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=457, Percent_Identity=31.5098468271335, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=457, Percent_Identity=31.5098468271335, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=30.8584686774942, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=435, Percent_Identity=28.5057471264368, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=28.5057471264368, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32566629, Length=444, Percent_Identity=27.027027027027, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=272, Percent_Identity=29.0441176470588, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=164, Percent_Identity=41.4634146341463, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17509919, Length=288, Percent_Identity=27.0833333333333, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17557151, Length=263, Percent_Identity=26.615969581749, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=65.1515151515152, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=450, Percent_Identity=29.3333333333333, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=450, Percent_Identity=29.3333333333333, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6320377, Length=347, Percent_Identity=30.5475504322767, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322937, Length=346, Percent_Identity=24.5664739884393, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=264, Percent_Identity=26.8939393939394, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=32.2175732217573, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=149, Percent_Identity=33.5570469798658, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=59.4936708860759, Blast_Score=486, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=59.4936708860759, Blast_Score=486, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=50.3856041131105, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45553807, Length=331, Percent_Identity=34.7432024169184, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI45553816, Length=331, Percent_Identity=34.7432024169184, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24651721, Length=331, Percent_Identity=34.7432024169184, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI17864154, Length=331, Percent_Identity=34.7432024169184, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24652838, Length=453, Percent_Identity=30.242825607064, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17137572, Length=453, Percent_Identity=30.242825607064, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=27.9642058165548, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=30.3630363036304, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=29.3103448275862, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24647046, Length=294, Percent_Identity=27.2108843537415, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24647048, Length=294, Percent_Identity=27.2108843537415, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=37.3493975903614, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43574; Mature: 43443

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKAQDYASIDAAPEEKER
CCCHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHC
GITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREH
CEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHH
ILLARQVGVDYIVVFLNKTDLVDDPELIDLVEMEVRELLSEYDYPGDDIPVIRGSALKAL
HHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHC
EGDPEQEKVIMELMDTIDEYIPTPVRETDKPFLMPVEDVFTITGRGTVASGRIDRGTVKI
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEECCCCCCCEEEE
GDEVEIIGLKPDVIKSTVTGLEMFRKTLDLGEAGDNVGVLLRGVNREQVERGQVLAKPGS
CCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHCCCEEECCCC
IQLHNKFKGEVYILTKEEGGRHTPFFSNYRPQFYFHTTDVTGVIELPDGVEMVMPGDNVT
EEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCEE
FEVDLIAPVAIEKGTKFTVREGGRTVGAGVVSEILD
EEEEEEEEEEECCCCEEEEECCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AEKEHYERTKPHVNIGTIGHVDHGKTTLTAAITKVLSEKGLAKAQDYASIDAAPEEKER
CCHHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHC
GITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREH
CEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHHH
ILLARQVGVDYIVVFLNKTDLVDDPELIDLVEMEVRELLSEYDYPGDDIPVIRGSALKAL
HHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHC
EGDPEQEKVIMELMDTIDEYIPTPVRETDKPFLMPVEDVFTITGRGTVASGRIDRGTVKI
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEECCCCCCCEEEE
GDEVEIIGLKPDVIKSTVTGLEMFRKTLDLGEAGDNVGVLLRGVNREQVERGQVLAKPGS
CCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHCCCEEECCCC
IQLHNKFKGEVYILTKEEGGRHTPFFSNYRPQFYFHTTDVTGVIELPDGVEMVMPGDNVT
EEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCEE
FEVDLIAPVAIEKGTKFTVREGGRTVGAGVVSEILD
EEEEEEEEEEECCCCEEEEECCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA