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The map label for this gene is comEC [H]

Identifier: 116494814

GI number: 116494814

Start: 1309920

End: 1310915

Strand: Direct

Name: comEC [H]

Synonym: LSEI_1325

Alternate gene names: 116494814

Gene position: 1309920-1310915 (Clockwise)

Preceding gene: 116494813

Following gene: 116494815

Centisome position: 45.24

GC content: 49.5

Gene sequence:

>996_bases
TTGTTGGAAAATGGCTTAGTTAGTTTAAGCCGTGTACCTGGCCAAATAGTCACTGGCGCTTTTCCAAGTTGGTTAGCACT
TATCGGTATTGTCGTGATGCTGACGGTGATTGTTGCTAAACGCTGGTTGGCGGCGGCTTGCTGGTTCGTGACTGTCTTTG
GCTGGTGTTGGTATCAGCCAAATTATCGGGTGGTCATGTTTGACGTTGGACAAGGGGACGCAATTTTGATTGAAACCCCT
TTTCATCAAGGTGCTTTGTTGATTGATACTGGTGGGCGAATTTTCGGGAAGACGACCAATCCACCTGTCCGACGAGCCAT
TGTGCCATATTTGCATGCTCGCGGCTATGCTCGGCTGGATACCTTATTACTGACGCATCCGGATATGGACCATGTTGGCG
ATGCCCCGTTGCTATCTCAGCTAATGCCAGTAGGTCGACTGATCACCACAGCGACAAGCGTTCAGCATCAAATGATTCAA
AAAGTTGCCAAGCAAGTGCAACATGTGCAGCTCGTGGCGGCTGATGACTGGTTGCGCATTGGACCCGCTAAATTTCAGGT
CGTATCACCGAAAGCGCAGACATCCGGCGATCCTACCAAGACAAACGCCGATTCAATTGTGCTCTATGGTAAAATAGGCA
ACAGCAGTTGGTTGTTAACTGGCGACGCCGAAAAAGAAGCGGAAGAAAAGAATATCATGCCGCGTCAGCTAACGGCCGAT
TATTTAAAAGTCGGCCATCATGGTTCGAAAACATCAAGTACCCCGGCATTGATCAATAGCTTGCATTTAAAAGCAGCACT
TATTTCCGCCGGTGTCGATAATCGGTATGGTCATCCCCATCCAGAAACCCTTGCAACGTTGGCTGCAGCCCACGTTCCAT
GGGCTAGCACTAGCGAGCGAGGAATGCTGTGGGTTGAGCCGGGGCAAAACAAACAGGAAGATCAGCTTTTTGGCTGGTTA
CCAACAAAGGAGCAAAGCAGCAATGCAGGTCCGTGA

Upstream 100 bases:

>100_bases
TTTTTAACTGGCTGTTAATGCCGATCTTTGAACTTGGGGTCATGCCAGGGCTTGCGATCGCGTTGCTTTGCCACACGCCA
CCTTTGACGATGGTTTGCAA

Downstream 100 bases:

>100_bases
TTTTATCAAACATCTTAAGAATGGCGAACGACCGCCGCTGACATTGATTCTCGGTGAAGAATCCGCATTGCGTCAGCAGG
CACAACACGCGATAGCCAGC

Product: metallo-beta-lactamase superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MLENGLVSLSRVPGQIVTGAFPSWLALIGIVVMLTVIVAKRWLAAACWFVTVFGWCWYQPNYRVVMFDVGQGDAILIETP
FHQGALLIDTGGRIFGKTTNPPVRRAIVPYLHARGYARLDTLLLTHPDMDHVGDAPLLSQLMPVGRLITTATSVQHQMIQ
KVAKQVQHVQLVAADDWLRIGPAKFQVVSPKAQTSGDPTKTNADSIVLYGKIGNSSWLLTGDAEKEAEEKNIMPRQLTAD
YLKVGHHGSKTSSTPALINSLHLKAALISAGVDNRYGHPHPETLATLAAAHVPWASTSERGMLWVEPGQNKQEDQLFGWL
PTKEQSSNAGP

Sequences:

>Translated_331_residues
MLENGLVSLSRVPGQIVTGAFPSWLALIGIVVMLTVIVAKRWLAAACWFVTVFGWCWYQPNYRVVMFDVGQGDAILIETP
FHQGALLIDTGGRIFGKTTNPPVRRAIVPYLHARGYARLDTLLLTHPDMDHVGDAPLLSQLMPVGRLITTATSVQHQMIQ
KVAKQVQHVQLVAADDWLRIGPAKFQVVSPKAQTSGDPTKTNADSIVLYGKIGNSSWLLTGDAEKEAEEKNIMPRQLTAD
YLKVGHHGSKTSSTPALINSLHLKAALISAGVDNRYGHPHPETLATLAAAHVPWASTSERGMLWVEPGQNKQEDQLFGWL
PTKEQSSNAGP
>Mature_331_residues
MLENGLVSLSRVPGQIVTGAFPSWLALIGIVVMLTVIVAKRWLAAACWFVTVFGWCWYQPNYRVVMFDVGQGDAILIETP
FHQGALLIDTGGRIFGKTTNPPVRRAIVPYLHARGYARLDTLLLTHPDMDHVGDAPLLSQLMPVGRLITTATSVQHQMIQ
KVAKQVQHVQLVAADDWLRIGPAKFQVVSPKAQTSGDPTKTNADSIVLYGKIGNSSWLLTGDAEKEAEEKNIMPRQLTAD
YLKVGHHGSKTSSTPALINSLHLKAALISAGVDNRYGHPHPETLATLAAAHVPWASTSERGMLWVEPGQNKQEDQLFGWL
PTKEQSSNAGP

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=250, Percent_Identity=28.8, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 36143; Mature: 36143

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENGLVSLSRVPGQIVTGAFPSWLALIGIVVMLTVIVAKRWLAAACWFVTVFGWCWYQP
CCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
NYRVVMFDVGQGDAILIETPFHQGALLIDTGGRIFGKTTNPPVRRAIVPYLHARGYARLD
CCEEEEEECCCCCEEEEECCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHCCHHHHH
TLLLTHPDMDHVGDAPLLSQLMPVGRLITTATSVQHQMIQKVAKQVQHVQLVAADDWLRI
EEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEE
GPAKFQVVSPKAQTSGDPTKTNADSIVLYGKIGNSSWLLTGDAEKEAEEKNIMPRQLTAD
CCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHCCCCHHHHHH
YLKVGHHGSKTSSTPALINSLHLKAALISAGVDNRYGHPHPETLATLAAAHVPWASTSER
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
GMLWVEPGQNKQEDQLFGWLPTKEQSSNAGP
CEEEECCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLENGLVSLSRVPGQIVTGAFPSWLALIGIVVMLTVIVAKRWLAAACWFVTVFGWCWYQP
CCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECC
NYRVVMFDVGQGDAILIETPFHQGALLIDTGGRIFGKTTNPPVRRAIVPYLHARGYARLD
CCEEEEEECCCCCEEEEECCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHHHCCHHHHH
TLLLTHPDMDHVGDAPLLSQLMPVGRLITTATSVQHQMIQKVAKQVQHVQLVAADDWLRI
EEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCEEE
GPAKFQVVSPKAQTSGDPTKTNADSIVLYGKIGNSSWLLTGDAEKEAEEKNIMPRQLTAD
CCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCHHHHHHHCCCCHHHHHH
YLKVGHHGSKTSSTPALINSLHLKAALISAGVDNRYGHPHPETLATLAAAHVPWASTSER
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
GMLWVEPGQNKQEDQLFGWLPTKEQSSNAGP
CEEEECCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]