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The map label for this gene is murD

Identifier: 116494762

GI number: 116494762

Start: 1249223

End: 1250602

Strand: Direct

Name: murD

Synonym: LSEI_1271

Alternate gene names: 116494762

Gene position: 1249223-1250602 (Clockwise)

Preceding gene: 116494761

Following gene: 116494763

Centisome position: 43.15

GC content: 47.83

Gene sequence:

>1380_bases
GTGAAGAATATTTCGGATTATCGCAATAAAAAAGTGCTTGTTTTGGGATTAGCGAAAAGTGGCGTGAATGCGGCACGCCT
ATTACATAAGTTGGGCGCTTTAGTGACAGTCAACGACAAACAACAATTTGACGACAACAAAGACGCTCAGGAACTGCTTG
CAGACGGTATGCGGGTCATTACCGGCAGGCATCCGGTTGAGCTCTTAGACGAGCATTTCGAGTTGATGGTCAAAAATCCT
GGCATTCCATACAGTAATCCGATGGTTAAGCGGGCTGAGGCGTTACATATGCCGATTATCACTGAACCCGAGTTGGCTTA
TCAGGTTTCGGAAGCTCAATGGATCGGGATTACTGGGACAAACGGTAAAACGACCACAACCACGCTGATTGGCTTGATGT
TGAATCAGCAGCGGCCACATCATGCGTTTGATGCGGGCAATATTGGCATTCCCGTTAGTCAAGTCGCTCAAAAAGTCGGC
AAAGACGATACGATTGTGGCTGAGTTGTCGAGTTTTCAACTGTGCGGCATCAAAACGTTGCATCCACATATTGCCGTTCT
AACGAATATTTATGAAGCTCATCTGGATTGGCATGGCAACCGGGCAAATTATGTTGCGGCCAAAATGCGAATTACCATGA
ATCAAACGCCAGACGACTATTTCATCATGAACTGGGATTTGCCAGAAATGCATGAGTTGGCGAAACAGAGCAAGGCACAA
ATCGTCCCGTTCTCAAGAAAGAACGCGGAAGGCGCTCGAGCTCAGTTAATCGATGGTTGGCTGACGTTTGATGGCGATCG
GATTATGAAGGCCAGCGAGATGCAGATCCCCGGCTTACATAATATCGAAAATGCGTTAGCTGCCATCGCTGCCGTGAAAC
TCGAAGGGGTCGGCGACGATGCTATTCGCGAAGTGCTGCGAACATTCTCCGGGGTTAAACATCGGATTCAATATCTAGAA
ACCATTGATGGCCGCCGGGTCTACAACGATTCAAAAGCGACTAACGTTGAGGCAGCCACCGTTGCCTTGAATGCATTCGA
CCAACCGATTGTCTGGCTGGCAGGTGGTTTGGATCGCGGATTGCCAATGGATGCACTGACACCACTGGTTAAGAAACACG
TTAAAAGCATGGTTGTTTTTGGTCAAACAGCACCCTTGATGGCCAAGATTGCTAAAGATGCCGGGGTACCAGTTCAAACG
ACTGAGAATGTGATGACTGCGGTGCCACTTGCCTATGAAGTCAGTCGCCCTGGTGATGTCATCTTACTTTCGCCAGCGGC
CGCAAGTTGGGACCAATATCCTAATTTTGAAGTTCGCGGCGATGATTTCATTAAAGCTGTCAATCAATTAAAAGCAACAG
TCGAAAGTGGGGATAAGTAA

Upstream 100 bases:

>100_bases
GCAAATTGATCTGACGTTCTGGTTGGTCGGGCTAATTGGCAGTGGCATCTATTTAGCATTTTTTCTTTAAAAATAAGGCA
TAAGCAAAGAAGGATAAATC

Downstream 100 bases:

>100_bases
ATGCGGCTGGTGATCTCTGGAGGCGGCACTGGTGGCCACATTTATCCGGCACTGGCGTTGATTGAAGCCTTAAAAGCGGA
AGGAAAACTTGACGATGTGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKNISDYRNKKVLVLGLAKSGVNAARLLHKLGALVTVNDKQQFDDNKDAQELLADGMRVITGRHPVELLDEHFELMVKNP
GIPYSNPMVKRAEALHMPIITEPELAYQVSEAQWIGITGTNGKTTTTTLIGLMLNQQRPHHAFDAGNIGIPVSQVAQKVG
KDDTIVAELSSFQLCGIKTLHPHIAVLTNIYEAHLDWHGNRANYVAAKMRITMNQTPDDYFIMNWDLPEMHELAKQSKAQ
IVPFSRKNAEGARAQLIDGWLTFDGDRIMKASEMQIPGLHNIENALAAIAAVKLEGVGDDAIREVLRTFSGVKHRIQYLE
TIDGRRVYNDSKATNVEAATVALNAFDQPIVWLAGGLDRGLPMDALTPLVKKHVKSMVVFGQTAPLMAKIAKDAGVPVQT
TENVMTAVPLAYEVSRPGDVILLSPAAASWDQYPNFEVRGDDFIKAVNQLKATVESGDK

Sequences:

>Translated_459_residues
MKNISDYRNKKVLVLGLAKSGVNAARLLHKLGALVTVNDKQQFDDNKDAQELLADGMRVITGRHPVELLDEHFELMVKNP
GIPYSNPMVKRAEALHMPIITEPELAYQVSEAQWIGITGTNGKTTTTTLIGLMLNQQRPHHAFDAGNIGIPVSQVAQKVG
KDDTIVAELSSFQLCGIKTLHPHIAVLTNIYEAHLDWHGNRANYVAAKMRITMNQTPDDYFIMNWDLPEMHELAKQSKAQ
IVPFSRKNAEGARAQLIDGWLTFDGDRIMKASEMQIPGLHNIENALAAIAAVKLEGVGDDAIREVLRTFSGVKHRIQYLE
TIDGRRVYNDSKATNVEAATVALNAFDQPIVWLAGGLDRGLPMDALTPLVKKHVKSMVVFGQTAPLMAKIAKDAGVPVQT
TENVMTAVPLAYEVSRPGDVILLSPAAASWDQYPNFEVRGDDFIKAVNQLKATVESGDK
>Mature_459_residues
MKNISDYRNKKVLVLGLAKSGVNAARLLHKLGALVTVNDKQQFDDNKDAQELLADGMRVITGRHPVELLDEHFELMVKNP
GIPYSNPMVKRAEALHMPIITEPELAYQVSEAQWIGITGTNGKTTTTTLIGLMLNQQRPHHAFDAGNIGIPVSQVAQKVG
KDDTIVAELSSFQLCGIKTLHPHIAVLTNIYEAHLDWHGNRANYVAAKMRITMNQTPDDYFIMNWDLPEMHELAKQSKAQ
IVPFSRKNAEGARAQLIDGWLTFDGDRIMKASEMQIPGLHNIENALAAIAAVKLEGVGDDAIREVLRTFSGVKHRIQYLE
TIDGRRVYNDSKATNVEAATVALNAFDQPIVWLAGGLDRGLPMDALTPLVKKHVKSMVVFGQTAPLMAKIAKDAGVPVQT
TENVMTAVPLAYEVSRPGDVILLSPAAASWDQYPNFEVRGDDFIKAVNQLKATVESGDK

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=458, Percent_Identity=30.3493449781659, Blast_Score=158, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_LACC3 (Q039R8)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806496.1
- ProteinModelPortal:   Q039R8
- SMR:   Q039R8
- STRING:   Q039R8
- GeneID:   4419567
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1271
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- BioCyc:   LCAS321967:LSEI_1271-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50491; Mature: 50491

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNISDYRNKKVLVLGLAKSGVNAARLLHKLGALVTVNDKQQFDDNKDAQELLADGMRVI
CCCCCHHCCCEEEEEEECCCCCHHHHHHHHHCCEEEECCHHHCCCCCCHHHHHHCCCEEE
TGRHPVELLDEHFELMVKNPGIPYSNPMVKRAEALHMPIITEPELAYQVSEAQWIGITGT
CCCCCHHHHHHHHHEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHEEECCCEEEEEECC
NGKTTTTTLIGLMLNQQRPHHAFDAGNIGIPVSQVAQKVGKDDTIVAELSSFQLCGIKTL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCEEECEEEC
HPHIAVLTNIYEAHLDWHGNRANYVAAKMRITMNQTPDDYFIMNWDLPEMHELAKQSKAQ
CHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHCCCE
IVPFSRKNAEGARAQLIDGWLTFDGDRIMKASEMQIPGLHNIENALAAIAAVKLEGVGDD
ECCCCCCCCCCCHHEEEECEEEECCCHHEECCCCCCCCCCHHHHHHHHHHHHEECCCCCH
AIREVLRTFSGVKHRIQYLETIDGRRVYNDSKATNVEAATVALNAFDQPIVWLAGGLDRG
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCC
LPMDALTPLVKKHVKSMVVFGQTAPLMAKIAKDAGVPVQTTENVMTAVPLAYEVSRPGDV
CCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCCCCCHHHHHCCEEEEECCCCCE
ILLSPAAASWDQYPNFEVRGDDFIKAVNQLKATVESGDK
EEECCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNISDYRNKKVLVLGLAKSGVNAARLLHKLGALVTVNDKQQFDDNKDAQELLADGMRVI
CCCCCHHCCCEEEEEEECCCCCHHHHHHHHHCCEEEECCHHHCCCCCCHHHHHHCCCEEE
TGRHPVELLDEHFELMVKNPGIPYSNPMVKRAEALHMPIITEPELAYQVSEAQWIGITGT
CCCCCHHHHHHHHHEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHEEECCCEEEEEECC
NGKTTTTTLIGLMLNQQRPHHAFDAGNIGIPVSQVAQKVGKDDTIVAELSSFQLCGIKTL
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCEEECEEEC
HPHIAVLTNIYEAHLDWHGNRANYVAAKMRITMNQTPDDYFIMNWDLPEMHELAKQSKAQ
CHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHCCCE
IVPFSRKNAEGARAQLIDGWLTFDGDRIMKASEMQIPGLHNIENALAAIAAVKLEGVGDD
ECCCCCCCCCCCHHEEEECEEEECCCHHEECCCCCCCCCCHHHHHHHHHHHHEECCCCCH
AIREVLRTFSGVKHRIQYLETIDGRRVYNDSKATNVEAATVALNAFDQPIVWLAGGLDRG
HHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCEEEEECCCCCC
LPMDALTPLVKKHVKSMVVFGQTAPLMAKIAKDAGVPVQTTENVMTAVPLAYEVSRPGDV
CCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHCCCCCCCCCHHHHHCCEEEEECCCCCE
ILLSPAAASWDQYPNFEVRGDDFIKAVNQLKATVESGDK
EEECCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA