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The map label for this gene is pbpB [H]

Identifier: 116494760

GI number: 116494760

Start: 1246065

End: 1248212

Strand: Direct

Name: pbpB [H]

Synonym: LSEI_1269

Alternate gene names: 116494760

Gene position: 1246065-1248212 (Clockwise)

Preceding gene: 116494759

Following gene: 116494761

Centisome position: 43.04

GC content: 46.97

Gene sequence:

>2148_bases
ATGAAAATTTTCAAATCAAAACGATCAACGAATGCATATCCTGCCCGAAACCGTAAAATTTTCGGCATCTGGATGATCGG
GGTCACACTCCTGATCCTTGGTGGGTTCGTTCTTCGTTTTTCTTTTGTGGCGACTGTTGGCGAGGTTGATTCTAACAATC
TGGCGAAGCAGCGACAAAAACAATATCAAACTGATACTGTCTTGCAGGCTAAGCGCGGCGCCATTCTCGATAAAACTGGC
AATGTGATCGCAGAAGATTCCAATACTTATACCATTTTTGCGATTTTGGACAAGCAAAGCAAAGACAGCAACAATAAACC
TGATTATGTGGTTGATAAAGCAAAAACGGCGAAGATTCTGAGTCGTTATTTGGCGATGTCAGAAGCAAAAATTTTGGCGC
GACTAACGCCAGGTAAAAATATGTATCAGGTTGAATTTGGCACACCTGGCACCAAATTGAGTTTGGCGATCAAGAAACAA
ATTGATGCTGAAAAACTGCCGGGCATCCATTTTCGTGAAACACCGTCGCGACTGTATCCAAATGGCGTCTTTGCGAGTCA
CGTGATTGGTCTGGCTCAGGCACAAGGCAAAAGTACCAGCTTGACAGGCGTCATGGGGCTTGAAAAGCAATTCAATACGG
TTTTAGCCGGTACGAATGGTTATCGTCGCAGCCGGACTGATGCATATGGCTATAAGCTGCCTAACGCAAAGACGAATCTG
AAAACGGCTGTTAATGGCGGTACCGTCTACACAACGCTCGATAGTGGTTTGCAGGCCTATTTAGAGACGCTGATGACCGA
CGTGCAAAATAAATATGAACCAAAAGGCATGACGGCAGTGTTGATGAGTGCTAAAACTGGACAGATTTTGGCAGCGACTC
AACGACCAACGTTTGATGCTTCAACGGGTGAAGGCTTAGGCGACATGTGGCGTGACTCGCTTGTTGAAGACAATTATGAA
CCAGGCTCGGTCATGAAAATTGTGACGCTGGCGGCTGCGATTCAAAGTGGCAAGTATCACCCAAATGATTATTATCAATC
CGGATCTATCAAATTGGATGGTGGTACTGTTAATGACTGGCGAACCGGCGGCTGGGGATCCATTCCTTTAAGCAAAGCCT
TCCCTCTGTCGAGTAACGTGGGGATGGTTAAGATTGAACAGGCAATGGGGGCTGGCATCTGGAAGCAGTATTTAGATAAA
TTTCGTTTCGGCCAGAAAACTGGCATCACCTTGCCGGGTGAAACTAGCGGGCACATTAGTTTTGAACGGCCCCTCGACCA
AGCAATCACCTCATTTGGTCAAGGCATTGAGGTCAATGTGATGCAGATGCTGCAGGCGATCAGTGCCGTTTCCAATGGCG
GCAAAATGTTGAAACCGCAGATTGTTTCTAAAGTCATCTCTCAGAACGGCAAAACCAAAGTTTATAAACCGGAAGTCGTT
GGGCAACCCATTTCAAAGGAAACTGCGGCCCAAGTCATTGATGCAATGCGGCATGTTGTTAACGATGAAGACGGCACCGG
TGTCGCATACAAGATGCCCGGTGTTGATCTGGCTGTGAAAACCGGGACGGGGCAAATTGCCAGTCCACAAGGTGGCTATC
TGACAGGTGATTCAAACTATACCTTCAGTGTTGCCGGGGTTGCGCCGGCTTCTGATCCACAGTACGTCCTTTATCTGGCC
ATGAAACAACCGCAAAAAATGACGGAACCGGCCGAATCCATTTTGGCTAGCATTTTTAAACCGATGATGCAGCGGGCACT
GGATACAACTGCTAATGGTGCGGTTACTGATGATACCAACACGGCCACCATTCCAGCGGTCACTAACGCGGCATTGACAA
CGGCTCAAGATAGCTTGAAGCAAGCCAATTTCGACGTGGCAACTGTGGGTACAGGTAATAAAGTTGTGCAACAATTACCA
ACAAGCGGTACCAAGGCACTGCACGGTTCCCGAGTTTTGCTTTTGACGAACGGGGCCATGACCATGCCTGACTTAACTGG
TTGGTCAAAATCTGATGTGTTAAAATTTGTACAACTGACCGGGAAGAAATTCAAACTTGTCGGTGATGGTTTTGTGACCC
AGCAAAGTATCGCTGCAGGCACATTGTTAGGCGATACTAGCGGGACCATTAAATTTAAACAACAATAG

Upstream 100 bases:

>100_bases
AGGAGATTGGTGAACTTTCAAATTCAGAACGCCTTGATGCCTTTGCCAAAGCACACGATTTTAAGGTTATCAACGGCAAC
ATAAAGCGTGTTGATAATAA

Downstream 100 bases:

>100_bases
AATTGGGGATAGTCATGCAACTTGCTCAGATGCTCATCCCGATGGTTTCGGCATTTGCAATTACCATTATGTTCATGCCA
TTGTTCATCGGCTACATGCG

Product: cell division protein FtsI/penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MKIFKSKRSTNAYPARNRKIFGIWMIGVTLLILGGFVLRFSFVATVGEVDSNNLAKQRQKQYQTDTVLQAKRGAILDKTG
NVIAEDSNTYTIFAILDKQSKDSNNKPDYVVDKAKTAKILSRYLAMSEAKILARLTPGKNMYQVEFGTPGTKLSLAIKKQ
IDAEKLPGIHFRETPSRLYPNGVFASHVIGLAQAQGKSTSLTGVMGLEKQFNTVLAGTNGYRRSRTDAYGYKLPNAKTNL
KTAVNGGTVYTTLDSGLQAYLETLMTDVQNKYEPKGMTAVLMSAKTGQILAATQRPTFDASTGEGLGDMWRDSLVEDNYE
PGSVMKIVTLAAAIQSGKYHPNDYYQSGSIKLDGGTVNDWRTGGWGSIPLSKAFPLSSNVGMVKIEQAMGAGIWKQYLDK
FRFGQKTGITLPGETSGHISFERPLDQAITSFGQGIEVNVMQMLQAISAVSNGGKMLKPQIVSKVISQNGKTKVYKPEVV
GQPISKETAAQVIDAMRHVVNDEDGTGVAYKMPGVDLAVKTGTGQIASPQGGYLTGDSNYTFSVAGVAPASDPQYVLYLA
MKQPQKMTEPAESILASIFKPMMQRALDTTANGAVTDDTNTATIPAVTNAALTTAQDSLKQANFDVATVGTGNKVVQQLP
TSGTKALHGSRVLLLTNGAMTMPDLTGWSKSDVLKFVQLTGKKFKLVGDGFVTQQSIAAGTLLGDTSGTIKFKQQ

Sequences:

>Translated_715_residues
MKIFKSKRSTNAYPARNRKIFGIWMIGVTLLILGGFVLRFSFVATVGEVDSNNLAKQRQKQYQTDTVLQAKRGAILDKTG
NVIAEDSNTYTIFAILDKQSKDSNNKPDYVVDKAKTAKILSRYLAMSEAKILARLTPGKNMYQVEFGTPGTKLSLAIKKQ
IDAEKLPGIHFRETPSRLYPNGVFASHVIGLAQAQGKSTSLTGVMGLEKQFNTVLAGTNGYRRSRTDAYGYKLPNAKTNL
KTAVNGGTVYTTLDSGLQAYLETLMTDVQNKYEPKGMTAVLMSAKTGQILAATQRPTFDASTGEGLGDMWRDSLVEDNYE
PGSVMKIVTLAAAIQSGKYHPNDYYQSGSIKLDGGTVNDWRTGGWGSIPLSKAFPLSSNVGMVKIEQAMGAGIWKQYLDK
FRFGQKTGITLPGETSGHISFERPLDQAITSFGQGIEVNVMQMLQAISAVSNGGKMLKPQIVSKVISQNGKTKVYKPEVV
GQPISKETAAQVIDAMRHVVNDEDGTGVAYKMPGVDLAVKTGTGQIASPQGGYLTGDSNYTFSVAGVAPASDPQYVLYLA
MKQPQKMTEPAESILASIFKPMMQRALDTTANGAVTDDTNTATIPAVTNAALTTAQDSLKQANFDVATVGTGNKVVQQLP
TSGTKALHGSRVLLLTNGAMTMPDLTGWSKSDVLKFVQLTGKKFKLVGDGFVTQQSIAAGTLLGDTSGTIKFKQQ
>Mature_715_residues
MKIFKSKRSTNAYPARNRKIFGIWMIGVTLLILGGFVLRFSFVATVGEVDSNNLAKQRQKQYQTDTVLQAKRGAILDKTG
NVIAEDSNTYTIFAILDKQSKDSNNKPDYVVDKAKTAKILSRYLAMSEAKILARLTPGKNMYQVEFGTPGTKLSLAIKKQ
IDAEKLPGIHFRETPSRLYPNGVFASHVIGLAQAQGKSTSLTGVMGLEKQFNTVLAGTNGYRRSRTDAYGYKLPNAKTNL
KTAVNGGTVYTTLDSGLQAYLETLMTDVQNKYEPKGMTAVLMSAKTGQILAATQRPTFDASTGEGLGDMWRDSLVEDNYE
PGSVMKIVTLAAAIQSGKYHPNDYYQSGSIKLDGGTVNDWRTGGWGSIPLSKAFPLSSNVGMVKIEQAMGAGIWKQYLDK
FRFGQKTGITLPGETSGHISFERPLDQAITSFGQGIEVNVMQMLQAISAVSNGGKMLKPQIVSKVISQNGKTKVYKPEVV
GQPISKETAAQVIDAMRHVVNDEDGTGVAYKMPGVDLAVKTGTGQIASPQGGYLTGDSNYTFSVAGVAPASDPQYVLYLA
MKQPQKMTEPAESILASIFKPMMQRALDTTANGAVTDDTNTATIPAVTNAALTTAQDSLKQANFDVATVGTGNKVVQQLP
TSGTKALHGSRVLLLTNGAMTMPDLTGWSKSDVLKFVQLTGKKFKLVGDGFVTQQSIAAGTLLGDTSGTIKFKQQ

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=594, Percent_Identity=25.9259259259259, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1786854, Length=505, Percent_Identity=23.1683168316832, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 76939; Mature: 76939

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFKSKRSTNAYPARNRKIFGIWMIGVTLLILGGFVLRFSFVATVGEVDSNNLAKQRQK
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QYQTDTVLQAKRGAILDKTGNVIAEDSNTYTIFAILDKQSKDSNNKPDYVVDKAKTAKIL
HHHHHHHHHHHCCCEEECCCCEEEECCCCEEEEEEEECCCCCCCCCCCEEECCHHHHHHH
SRYLAMSEAKILARLTPGKNMYQVEFGTPGTKLSLAIKKQIDAEKLPGIHFRETPSRLYP
HHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEEEEEHHHCCHHHCCCCCEECCCHHHCC
NGVFASHVIGLAQAQGKSTSLTGVMGLEKQFNTVLAGTNGYRRSRTDAYGYKLPNAKTNL
CCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHCEEEECCCCCCCCCCCCCCEECCCCCCCC
KTAVNGGTVYTTLDSGLQAYLETLMTDVQNKYEPKGMTAVLMSAKTGQILAATQRPTFDA
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCC
STGEGLGDMWRDSLVEDNYEPGSVMKIVTLAAAIQSGKYHPNDYYQSGSIKLDGGTVNDW
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCC
RTGGWGSIPLSKAFPLSSNVGMVKIEQAMGAGIWKQYLDKFRFGQKTGITLPGETSGHIS
CCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
FERPLDQAITSFGQGIEVNVMQMLQAISAVSNGGKMLKPQIVSKVISQNGKTKVYKPEVV
ECCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCHHC
GQPISKETAAQVIDAMRHVVNDEDGTGVAYKMPGVDLAVKTGTGQIASPQGGYLTGDSNY
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEECCCCE
TFSVAGVAPASDPQYVLYLAMKQPQKMTEPAESILASIFKPMMQRALDTTANGAVTDDTN
EEEEEECCCCCCCCEEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
TATIPAVTNAALTTAQDSLKQANFDVATVGTGNKVVQQLPTSGTKALHGSRVLLLTNGAM
CEEECCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCEEEEEECCCE
TMPDLTGWSKSDVLKFVQLTGKKFKLVGDGFVTQQSIAAGTLLGDTSGTIKFKQQ
ECCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEHHHHHHEEEEECCCCCEEEECC
>Mature Secondary Structure
MKIFKSKRSTNAYPARNRKIFGIWMIGVTLLILGGFVLRFSFVATVGEVDSNNLAKQRQK
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
QYQTDTVLQAKRGAILDKTGNVIAEDSNTYTIFAILDKQSKDSNNKPDYVVDKAKTAKIL
HHHHHHHHHHHCCCEEECCCCEEEECCCCEEEEEEEECCCCCCCCCCCEEECCHHHHHHH
SRYLAMSEAKILARLTPGKNMYQVEFGTPGTKLSLAIKKQIDAEKLPGIHFRETPSRLYP
HHHHHHHHHHEEEEECCCCCEEEEEECCCCCEEEEEEHHHCCHHHCCCCCEECCCHHHCC
NGVFASHVIGLAQAQGKSTSLTGVMGLEKQFNTVLAGTNGYRRSRTDAYGYKLPNAKTNL
CCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHCEEEECCCCCCCCCCCCCCEECCCCCCCC
KTAVNGGTVYTTLDSGLQAYLETLMTDVQNKYEPKGMTAVLMSAKTGQILAATQRPTFDA
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCCCCCC
STGEGLGDMWRDSLVEDNYEPGSVMKIVTLAAAIQSGKYHPNDYYQSGSIKLDGGTVNDW
CCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCC
RTGGWGSIPLSKAFPLSSNVGMVKIEQAMGAGIWKQYLDKFRFGQKTGITLPGETSGHIS
CCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEE
FERPLDQAITSFGQGIEVNVMQMLQAISAVSNGGKMLKPQIVSKVISQNGKTKVYKPEVV
ECCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEECCHHC
GQPISKETAAQVIDAMRHVVNDEDGTGVAYKMPGVDLAVKTGTGQIASPQGGYLTGDSNY
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEECCCCE
TFSVAGVAPASDPQYVLYLAMKQPQKMTEPAESILASIFKPMMQRALDTTANGAVTDDTN
EEEEEECCCCCCCCEEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
TATIPAVTNAALTTAQDSLKQANFDVATVGTGNKVVQQLPTSGTKALHGSRVLLLTNGAM
CEEECCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCCCEEEEEECCCE
TMPDLTGWSKSDVLKFVQLTGKKFKLVGDGFVTQQSIAAGTLLGDTSGTIKFKQQ
ECCCCCCCCHHHHHHHHHHCCCEEEEEECCCEEHHHHHHEEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]