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The map label for this gene is purB [H]

Identifier: 116494619

GI number: 116494619

Start: 1104976

End: 1106271

Strand: Direct

Name: purB [H]

Synonym: LSEI_1120

Alternate gene names: 116494619

Gene position: 1104976-1106271 (Clockwise)

Preceding gene: 116494618

Following gene: 116494623

Centisome position: 38.16

GC content: 50.39

Gene sequence:

>1296_bases
GTGATTCCGCGTTATAGTCGTCCCGAAATGGCCGCAATCTGGTCAGAGAAGAACCGTTATGCTGCATGGCTGGAAGTTGA
AATCTTAGCTGCCGAAGCCTGGTCCAAACTAGGGGAAATTCCCGCTGCTGATGTCCAAGCATTGCGTGCGCATGCTAAGT
TTGATGTTGATCGGATTCATGAGATTGAAGCGGTCACGCATCATGACGTGGTGGCATTCACGCGGGATGTTTCTGAAAGC
CTCGGCGCTGAGAAGAAATGGGTTCATTTTGGCTTAACCAGTACCGATGTTGTCGACACGGCACAGGGCTACCTGTTGAA
GCAGGCTAACGAGATTCTGCGTGCAGATTTGGCCGCCTTGAAGGCTAGTTTAGCAAAGTTGGCGAAGGCCCATAAGGATA
CCGTGATGATGGGGCGGACGCATGGCGTGCACGCTGAACCAACTACTTTTGGGTTGGTTGTTGCCACGTGGTATTCTGAA
TTGAAGCGAGACATCCGTCGATTTGAAGACGCGGCTAGTGATGTTGAAGCAGGCAAGATTAGCGGTGCAGTCGGTACCTT
TGCCAATACACCGCCTGCTGTTGAGGCGTACGTCACAGAGCAGTTGGGTATTCGCGCTCAAGAAATCTCGACTCAGATTC
TGCCGCGTGATCTGCATGCGCATTACATTCAGACACTTGGCCTGATTGCGACTTCGGTGGAACGATTTGCTTTGCAGATT
CGGCATATGCAGCGTACCGAAGTTCATGAAGTCGAAGAGCATTTCAACAAAGGTCAAAAGGGCTCGAGTGCTATGCCACA
CAAGCGCAATCCAATCGGTTCGGAAAATGTTTCTGGCTTGGCGCGCGTCATTCGTGGATACGAGACGCCAGCCTTAGAAG
ATGTGCTGTTGTGGCATGAACGCGATATCTCGCATTCCAGTGCCGAACGGATCATGTTGCCAGATGCTACCGGGCTGCTT
GATTACATTCTCCATCGATTCACCAGTATTTTGGACAATTTGGATGTTTTCCCAGACCGAATGAAAGCCAATATGAATCT
GACGCATGGCCTGATTTACAGCCAGCGGGTGATGCTGAAGCTGATCGAAGCTGGCTTGAGCCGAGAACAAGCCTATGACT
TGGTTCAACCCAAGACAGCGGTGGCATGGGATGAGCATCGCGCCTTCCGTCCGTTGCTTGAAGCTGATCCGAAAATTACT
GATCGGTTAAGTCAGGCCGATTTAGACGATGCTTTCGACTACCACTATCACCTCAAGCATGTTGATGAGATTTTCAAACG
AGTTGGTTTAGCATAA

Upstream 100 bases:

>100_bases
GAATGCGACTGGCCACTTTGTCATTCAATGATCGCGTGCTCGCAGTCGCAAACCAAGGATACCTGTTGTTGTTTTGTCCA
CTTGAAGGAGGAAAAAGCCT

Downstream 100 bases:

>100_bases
GCAAATAAATCAAACAAAAACGGCGAGTGCAGACTCGTCGTTTTTGTTTGGCCGCATTTTGTGCCACCTGTTGACCATAG
AAAAAGTCCTGTTCAAGTTG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIPRYSRPEMAAIWSEKNRYAAWLEVEILAAEAWSKLGEIPAADVQALRAHAKFDVDRIHEIEAVTHHDVVAFTRDVSES
LGAEKKWVHFGLTSTDVVDTAQGYLLKQANEILRADLAALKASLAKLAKAHKDTVMMGRTHGVHAEPTTFGLVVATWYSE
LKRDIRRFEDAASDVEAGKISGAVGTFANTPPAVEAYVTEQLGIRAQEISTQILPRDLHAHYIQTLGLIATSVERFALQI
RHMQRTEVHEVEEHFNKGQKGSSAMPHKRNPIGSENVSGLARVIRGYETPALEDVLLWHERDISHSSAERIMLPDATGLL
DYILHRFTSILDNLDVFPDRMKANMNLTHGLIYSQRVMLKLIEAGLSREQAYDLVQPKTAVAWDEHRAFRPLLEADPKIT
DRLSQADLDDAFDYHYHLKHVDEIFKRVGLA

Sequences:

>Translated_431_residues
MIPRYSRPEMAAIWSEKNRYAAWLEVEILAAEAWSKLGEIPAADVQALRAHAKFDVDRIHEIEAVTHHDVVAFTRDVSES
LGAEKKWVHFGLTSTDVVDTAQGYLLKQANEILRADLAALKASLAKLAKAHKDTVMMGRTHGVHAEPTTFGLVVATWYSE
LKRDIRRFEDAASDVEAGKISGAVGTFANTPPAVEAYVTEQLGIRAQEISTQILPRDLHAHYIQTLGLIATSVERFALQI
RHMQRTEVHEVEEHFNKGQKGSSAMPHKRNPIGSENVSGLARVIRGYETPALEDVLLWHERDISHSSAERIMLPDATGLL
DYILHRFTSILDNLDVFPDRMKANMNLTHGLIYSQRVMLKLIEAGLSREQAYDLVQPKTAVAWDEHRAFRPLLEADPKIT
DRLSQADLDDAFDYHYHLKHVDEIFKRVGLA
>Mature_431_residues
MIPRYSRPEMAAIWSEKNRYAAWLEVEILAAEAWSKLGEIPAADVQALRAHAKFDVDRIHEIEAVTHHDVVAFTRDVSES
LGAEKKWVHFGLTSTDVVDTAQGYLLKQANEILRADLAALKASLAKLAKAHKDTVMMGRTHGVHAEPTTFGLVVATWYSE
LKRDIRRFEDAASDVEAGKISGAVGTFANTPPAVEAYVTEQLGIRAQEISTQILPRDLHAHYIQTLGLIATSVERFALQI
RHMQRTEVHEVEEHFNKGQKGSSAMPHKRNPIGSENVSGLARVIRGYETPALEDVLLWHERDISHSSAERIMLPDATGLL
DYILHRFTSILDNLDVFPDRMKANMNLTHGLIYSQRVMLKLIEAGLSREQAYDLVQPKTAVAWDEHRAFRPLLEADPKIT
DRLSQADLDDAFDYHYHLKHVDEIFKRVGLA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=404, Percent_Identity=28.2178217821782, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4557269, Length=392, Percent_Identity=27.8061224489796, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI1787376, Length=297, Percent_Identity=30.3030303030303, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=421, Percent_Identity=26.603325415677, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=330, Percent_Identity=27.5757575757576, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=390, Percent_Identity=29.4871794871795, Blast_Score=159, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24647570, Length=387, Percent_Identity=26.0981912144703, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48511; Mature: 48511

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYSRPEMAAIWSEKNRYAAWLEVEILAAEAWSKLGEIPAADVQALRAHAKFDVDRIH
CCCCCCCCCHHHHHCCCCCEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
EIEAVTHHDVVAFTRDVSESLGAEKKWVHFGLTSTDVVDTAQGYLLKQANEILRADLAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KASLAKLAKAHKDTVMMGRTHGVHAEPTTFGLVVATWYSELKRDIRRFEDAASDVEAGKI
HHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAVGTFANTPPAVEAYVTEQLGIRAQEISTQILPRDLHAHYIQTLGLIATSVERFALQI
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHMQRTEVHEVEEHFNKGQKGSSAMPHKRNPIGSENVSGLARVIRGYETPALEDVLLWHE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
RDISHSSAERIMLPDATGLLDYILHRFTSILDNLDVFPDRMKANMNLTHGLIYSQRVMLK
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHH
LIEAGLSREQAYDLVQPKTAVAWDEHRAFRPLLEADPKITDRLSQADLDDAFDYHYHLKH
HHHHHCCHHHHHHHCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHH
VDEIFKRVGLA
HHHHHHHHCCC
>Mature Secondary Structure
MIPRYSRPEMAAIWSEKNRYAAWLEVEILAAEAWSKLGEIPAADVQALRAHAKFDVDRIH
CCCCCCCCCHHHHHCCCCCEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
EIEAVTHHDVVAFTRDVSESLGAEKKWVHFGLTSTDVVDTAQGYLLKQANEILRADLAAL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KASLAKLAKAHKDTVMMGRTHGVHAEPTTFGLVVATWYSELKRDIRRFEDAASDVEAGKI
HHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAVGTFANTPPAVEAYVTEQLGIRAQEISTQILPRDLHAHYIQTLGLIATSVERFALQI
CCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHMQRTEVHEVEEHFNKGQKGSSAMPHKRNPIGSENVSGLARVIRGYETPALEDVLLWHE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHH
RDISHSSAERIMLPDATGLLDYILHRFTSILDNLDVFPDRMKANMNLTHGLIYSQRVMLK
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHH
LIEAGLSREQAYDLVQPKTAVAWDEHRAFRPLLEADPKITDRLSQADLDDAFDYHYHLKH
HHHHHCCHHHHHHHCCCCHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHH
VDEIFKRVGLA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]