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The map label for this gene is cspLA [H]

Identifier: 116494613

GI number: 116494613

Start: 1100107

End: 1100307

Strand: Direct

Name: cspLA [H]

Synonym: LSEI_1114

Alternate gene names: 116494613

Gene position: 1100107-1100307 (Clockwise)

Preceding gene: 116494612

Following gene: 116494614

Centisome position: 38.0

GC content: 47.26

Gene sequence:

>201_bases
ATGGAACACGGCACAGTTAAATGGTTCAATGCTGAAAAGGGTTACGGTTTTATCACTCGCGAAGATGGTAGCGATGTTTT
CGTTCACTTCTCAGCTATCCAAGGCGACGGCTACAAGACCCTCGAAGAAGGCCAAGCAGTCACTTTTGAAGTTGAAGACA
GCGATCGCGGCCCACAAGCGGTTAACGTTAACAAAGACTAA

Upstream 100 bases:

>100_bases
AATGGTCGTGGTTGCATGGTTTCAGAACTTTCTTCCACTTGTACCAAACAGAAATACACGATATTATCATGCGTCAGCAG
ACGCAAGGAGGAATGTTCTC

Downstream 100 bases:

>100_bases
TATGAAAGTTTCAGAGCTGGTTGCAGAGATGCAACCGGCTTTTTATTTTCGCGTGTGCACGAGGACAGATACAGGTAAAA
TGGGTGAGTAGCGATGTTGC

Product: cold shock protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MEHGTVKWFNAEKGYGFITREDGSDVFVHFSAIQGDGYKTLEEGQAVTFEVEDSDRGPQAVNVNKD

Sequences:

>Translated_66_residues
MEHGTVKWFNAEKGYGFITREDGSDVFVHFSAIQGDGYKTLEEGQAVTFEVEDSDRGPQAVNVNKD
>Mature_66_residues
MEHGTVKWFNAEKGYGFITREDGSDVFVHFSAIQGDGYKTLEEGQAVTFEVEDSDRGPQAVNVNKD

Specific function: Unknown

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1788126, Length=61, Percent_Identity=70.4918032786885, Blast_Score=89, Evalue=5e-20,
Organism=Escherichia coli, GI1789979, Length=61, Percent_Identity=68.8524590163934, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=67.2131147540984, Blast_Score=85, Evalue=8e-19,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=67.2131147540984, Blast_Score=85, Evalue=9e-19,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=62.2950819672131, Blast_Score=81, Evalue=2e-17,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=63.9344262295082, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1787107, Length=61, Percent_Identity=55.7377049180328, Blast_Score=71, Evalue=1e-14,

Paralogues:

None

Copy number: 8300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7355; Mature: 7355

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHGTVKWFNAEKGYGFITREDGSDVFVHFSAIQGDGYKTLEEGQAVTFEVEDSDRGPQA
CCCCCEEEEECCCCCEEEEEECCCEEEEEEEEEECCCCEECCCCCEEEEEECCCCCCCEE
VNVNKD
EECCCC
>Mature Secondary Structure
MEHGTVKWFNAEKGYGFITREDGSDVFVHFSAIQGDGYKTLEEGQAVTFEVEDSDRGPQA
CCCCCEEEEECCCCCEEEEEECCCEEEEEEEEEECCCCEECCCCCEEEEEECCCCCCCEE
VNVNKD
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]