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The map label for this gene is dagK [H]

Identifier: 116494562

GI number: 116494562

Start: 1044009

End: 1045043

Strand: Direct

Name: dagK [H]

Synonym: LSEI_1060

Alternate gene names: 116494562

Gene position: 1044009-1045043 (Clockwise)

Preceding gene: 116494561

Following gene: 116494563

Centisome position: 36.06

GC content: 47.73

Gene sequence:

>1035_bases
ATGAGAAAACGGGCGCGTTTGATATACAACCCTACATCCGGCAACGAAGGTTTAAAGCGCTTCGTCCCCGACATTTTGGA
TATCATGGAACAGGCAGGATATGAAAGTTCAACCTTTCAAACAACCCCGAAGCCTTTTTCTGCACGTGAAGAAGCAAAGC
GTGCCACTGAAGCGGGTTTCGACTTAATTGTCGCTGCTGGTGGCGATGGTACGATTAATGAAGTGGTTAATGGGATCGCA
CCAGCTAAAAAACGCCCCAAAATGGCGATTATTCCAGCAGGGACGACCAATGACTACGCGCGTGCTTTGCGGATTTCGCG
GGATGATCCGGTTGAGGCGGCCCAAGTGATTCTCAAAGGCCAAACCCTTGCGATGGATATTGGCCAAGCCAATCATCATT
ATTTTATGAACATCGCGGCTGGTGGTTTGTTGAGCGAACTCACTTATTCTGTGCCATCTGAAGTTAAATCTATTTTTGGC
TATTTTGCCTATGTGATTAAAGGCGCCGAGATGTTACCAGCGGTGCGGACGGTGCCGATGAAGCTGGAATATGATGGCGG
GGTTTATGATGGACCCGCCAGCATGTTTTTCCTCGGCCTTACCAATTCTGTGGGCGGTTTTGAGCAGATCGTGCCGGATG
CTGCACTTGGTGATGGCAAGTTCTCCTTAATTATTGTTAAAACAGCGAACATGGCGAATTTGCTGAAGCTAATGGCGCTT
GTCTTCAATGGTGGTCGCCACGTGGATGATCCCAATATTATCTATACAAAGACTAAAAAGTTGCGGGTGAAGGCCGGCGG
AGACGATCCGTTGAAAATTAACCTTGATGGCGAATATGGCGGTGATGCGCCGATGACCTTTGTTAATTTGAAGCAACATA
TTGCCATGTATGCTAATCTAGACGAGATCCCGACCAAGAATCTTGGCACGGACGCGCAGAAGCAGAAGGACTACATGGCC
GAAGTCGAATCTATTTCTCATCAAGATATTGATGGTGATGGACAGATCGGCTCACAAGACGAAAAGGATGACTAA

Upstream 100 bases:

>100_bases
TGAGCAAGACATTGACGATTCACGATTATGACAAAGACTGTTTCCGCCAGCAACGGGCCAGCAGTCCAGTATTGTTAAAA
CGTTTAAGGAGCATTGACCG

Downstream 100 bases:

>100_bases
TTTGGCCCAACCAGCTGTAACCAAGAACCAAGATCTGGAAGTTACCATTCAAGATCTCACTTATGAAGGGATGGGCGTTG
CCAAAGTCGACGGCTTTCCG

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MRKRARLIYNPTSGNEGLKRFVPDILDIMEQAGYESSTFQTTPKPFSAREEAKRATEAGFDLIVAAGGDGTINEVVNGIA
PAKKRPKMAIIPAGTTNDYARALRISRDDPVEAAQVILKGQTLAMDIGQANHHYFMNIAAGGLLSELTYSVPSEVKSIFG
YFAYVIKGAEMLPAVRTVPMKLEYDGGVYDGPASMFFLGLTNSVGGFEQIVPDAALGDGKFSLIIVKTANMANLLKLMAL
VFNGGRHVDDPNIIYTKTKKLRVKAGGDDPLKINLDGEYGGDAPMTFVNLKQHIAMYANLDEIPTKNLGTDAQKQKDYMA
EVESISHQDIDGDGQIGSQDEKDD

Sequences:

>Translated_344_residues
MRKRARLIYNPTSGNEGLKRFVPDILDIMEQAGYESSTFQTTPKPFSAREEAKRATEAGFDLIVAAGGDGTINEVVNGIA
PAKKRPKMAIIPAGTTNDYARALRISRDDPVEAAQVILKGQTLAMDIGQANHHYFMNIAAGGLLSELTYSVPSEVKSIFG
YFAYVIKGAEMLPAVRTVPMKLEYDGGVYDGPASMFFLGLTNSVGGFEQIVPDAALGDGKFSLIIVKTANMANLLKLMAL
VFNGGRHVDDPNIIYTKTKKLRVKAGGDDPLKINLDGEYGGDAPMTFVNLKQHIAMYANLDEIPTKNLGTDAQKQKDYMA
EVESISHQDIDGDGQIGSQDEKDD
>Mature_344_residues
MRKRARLIYNPTSGNEGLKRFVPDILDIMEQAGYESSTFQTTPKPFSAREEAKRATEAGFDLIVAAGGDGTINEVVNGIA
PAKKRPKMAIIPAGTTNDYARALRISRDDPVEAAQVILKGQTLAMDIGQANHHYFMNIAAGGLLSELTYSVPSEVKSIFG
YFAYVIKGAEMLPAVRTVPMKLEYDGGVYDGPASMFFLGLTNSVGGFEQIVPDAALGDGKFSLIIVKTANMANLLKLMAL
VFNGGRHVDDPNIIYTKTKKLRVKAGGDDPLKINLDGEYGGDAPMTFVNLKQHIAMYANLDEIPTKNLGTDAQKQKDYMA
EVESISHQDIDGDGQIGSQDEKDD

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=258, Percent_Identity=29.4573643410853, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 37304; Mature: 37304

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRARLIYNPTSGNEGLKRFVPDILDIMEQAGYESSTFQTTPKPFSAREEAKRATEAGF
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DLIVAAGGDGTINEVVNGIAPAKKRPKMAIIPAGTTNDYARALRISRDDPVEAAQVILKG
EEEEEECCCCCHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCC
QTLAMDIGQANHHYFMNIAAGGLLSELTYSVPSEVKSIFGYFAYVIKGAEMLPAVRTVPM
CEEEEECCCCCCEEEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHCCHHHCCEE
KLEYDGGVYDGPASMFFLGLTNSVGGFEQIVPDAALGDGKFSLIIVKTANMANLLKLMAL
EEEECCCEECCCHHEEEEECCCCCCCHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
VFNGGRHVDDPNIIYTKTKKLRVKAGGDDPLKINLDGEYGGDAPMTFVNLKQHIAMYANL
HHCCCCCCCCCCEEEEECEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCH
DEIPTKNLGTDAQKQKDYMAEVESISHQDIDGDGQIGSQDEKDD
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRKRARLIYNPTSGNEGLKRFVPDILDIMEQAGYESSTFQTTPKPFSAREEAKRATEAGF
CCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
DLIVAAGGDGTINEVVNGIAPAKKRPKMAIIPAGTTNDYARALRISRDDPVEAAQVILKG
EEEEEECCCCCHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCC
QTLAMDIGQANHHYFMNIAAGGLLSELTYSVPSEVKSIFGYFAYVIKGAEMLPAVRTVPM
CEEEEECCCCCCEEEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHCCHHHCCEE
KLEYDGGVYDGPASMFFLGLTNSVGGFEQIVPDAALGDGKFSLIIVKTANMANLLKLMAL
EEEECCCEECCCHHEEEEECCCCCCCHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
VFNGGRHVDDPNIIYTKTKKLRVKAGGDDPLKINLDGEYGGDAPMTFVNLKQHIAMYANL
HHCCCCCCCCCCEEEEECEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCH
DEIPTKNLGTDAQKQKDYMAEVESISHQDIDGDGQIGSQDEKDD
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA