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The map label for this gene is ligA

Identifier: 116494557

GI number: 116494557

Start: 1037313

End: 1039337

Strand: Direct

Name: ligA

Synonym: LSEI_1055

Alternate gene names: 116494557

Gene position: 1037313-1039337 (Clockwise)

Preceding gene: 116494556

Following gene: 116494558

Centisome position: 35.83

GC content: 49.63

Gene sequence:

>2025_bases
GTGTTAGCAAAACCAGCTGCACAATTCACACTTGAAGAAGCTCAGGCGGAAGTCGAAAAGCTTCGGAAGCAACTGAATCA
ATGGCGTCTTGAGTATTACACCAAGGATGCGCCAACTGTGACCGATAACGTCTATGACGATCATTATCGCGATTTACAGG
CACTCGAGGAGGCTTATCCCAAGCTAGTCACGCCTGATTCGCCAACTCAAGAAGTCGGCGATGTCATTAATTCGGACTTT
GCCAAGGTGCAACACCCAATTCCGATGTTGTCGATGGGCGATGTTTTTTCGTTCGAAGAGCTGAGTGAATGGGATGCTCG
CATGCAAAGTAATGTCGGCCATCCGGTGGATTACAATGTTGAGTTGAAAATCGACGGGTTGGCTTTATCTCTGATTTATG
AAAACGGCAAACTCGTTCAAGGCTCAACACGTGGTGATGGTAATGTCGGTGAAGATGTGACCAGAAATGTGTTGACCATT
GCGTCAGTTCCCAAGCAGTTAAAGCAGCCACTTTCACTCGAGGTACGGGGTGAATGTTATATGCCTAAGGCGGCGTTTGC
CAAGCTGAATGCGCGGCAGGAAACTGAGGGCGGCACGCCTTTTGCCAATCCACGTAACGCGGCAGCTGGATCACTCCGAC
AGTTAGATGCCAAAGTCACCGCTGCTCGAGAGCTGGATACCTTCATTTATACGTTAATTGAACCAGAGCAGTTTAACGTG
ACCACACAGCATGAGGCGATTGCGTTTATGCAGGCACTTGGATTTACAACAAATCCAAGTTCAGAAGTGGCTGGTGACAT
GCAGGCGATTGACACCTACATTAAGAAGTACACCACCGACCGTGATGCGTTGCCTTACGGAATTGATGGGATCGTTTTGA
AAGTGAACGATTTAGCGCTGCAAGCGCAACTAGGTAACACCGTTAAAGTGCCACGCTGGGAAATTGCCTACAAATTCCCA
CCTGAAGAAGCAGAGACCGTCATTCATGACATTGTCTGGACAGTTGGTCGAACCGGTGTGGTCACGCCGACTGCAGTGAT
GGACCCCGTTCAGCTTGCTGGAACCACAGTGGCACGTGCCACGTTGCACAACGCGGATATGATTCGTGATAAAGATATTC
GCATCGGCGACACCGTCATGTTGCACAAAGCAGGCGACATTATTCCTGAAGTTTCGCGGGTACTGGTTGCTAAACGTCCA
GCGGATACGCCACCGGCGCCGATTCCAGAGGTCTGCCCGTCATGTGGCCAAAAACTGGTCCATCTCGACGATGAAGTAGC
TTTGCGTTGCATCAACCCGATGTGTCCGGCACAGGTCCAAGAGCAGCTCACCCATTTTGCGTCTCGCAACGCGATGAATA
TCGATGGCTTAGGCCCGAAGATTGTGGCACAGTTGCAGGCCAAACATCTGGTAAAAGATGTCGCTGATTTGTATCACCTC
ACAGCTGCGGATCTGGCGAAATTAGATAAGTTTAAAGAAAAATCAATTAATAATTTGTTAAGTGCGATCAACAACAGTCG
GCAAAACTCGGTAGAACGCCTAATCTTTGGGTTGGGCATTCGGCACGTTGGCGGTAAAGCCGCACGAATTCTGGCCGAGC
ATTTCGGCGATTTGGATCATCTGATGACCGCCAGTCAAGAGGCGATCGCAGATGTGCCAAATATCGGGCCGACCATTGGG
GAAGCGATCGTGACGTATTTTAAAGCCGCAACGGTTCAAAAGTTGATCACTCAGTTGCGCGAAGCTGATGTTAACCTGCG
TTACACCGGACCGACCAAACCAGTGGTCAAAGATAGTTTTGTTGCAGGTAAAACCGTTGTGATTACCGGTAAGTTTGCCG
AGTTTTCGCGGCCAGCACTCACGAAGCAGCTAGAAGGATTAGGAGCCAAGGTCACAGGTTCGGTTTCAAAGAAAACGGAC
ATCCTGATCGCTGGCGACGCGGCTGGCAGTAAGTTGGCAAAGGCACAAAGCTTGAATGTCCCAATCATGAATGAGACGGA
ATTGCTGGCGAATTTGAAGGATTGA

Upstream 100 bases:

>100_bases
GACTGCTAGCTGCCTTTGCTCCAATTGTCAAAGTGGCATCATAAGCGTTCGCAGTTTGACTCAGAAATGTCGGGATGCAT
ATTTTTAGAGGAGGCTCGCT

Downstream 100 bases:

>100_bases
GGAACATCAATGAAGAAATTTTTAACGTTAACTTTGCTGGCTGGGGCTGCGGTGTTGTTAGCAGCCTGTGGCAAGTTGAA
TTTTGACAGTGGCAGCAGCA

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MLAKPAAQFTLEEAQAEVEKLRKQLNQWRLEYYTKDAPTVTDNVYDDHYRDLQALEEAYPKLVTPDSPTQEVGDVINSDF
AKVQHPIPMLSMGDVFSFEELSEWDARMQSNVGHPVDYNVELKIDGLALSLIYENGKLVQGSTRGDGNVGEDVTRNVLTI
ASVPKQLKQPLSLEVRGECYMPKAAFAKLNARQETEGGTPFANPRNAAAGSLRQLDAKVTAARELDTFIYTLIEPEQFNV
TTQHEAIAFMQALGFTTNPSSEVAGDMQAIDTYIKKYTTDRDALPYGIDGIVLKVNDLALQAQLGNTVKVPRWEIAYKFP
PEEAETVIHDIVWTVGRTGVVTPTAVMDPVQLAGTTVARATLHNADMIRDKDIRIGDTVMLHKAGDIIPEVSRVLVAKRP
ADTPPAPIPEVCPSCGQKLVHLDDEVALRCINPMCPAQVQEQLTHFASRNAMNIDGLGPKIVAQLQAKHLVKDVADLYHL
TAADLAKLDKFKEKSINNLLSAINNSRQNSVERLIFGLGIRHVGGKAARILAEHFGDLDHLMTASQEAIADVPNIGPTIG
EAIVTYFKAATVQKLITQLREADVNLRYTGPTKPVVKDSFVAGKTVVITGKFAEFSRPALTKQLEGLGAKVTGSVSKKTD
ILIAGDAAGSKLAKAQSLNVPIMNETELLANLKD

Sequences:

>Translated_674_residues
MLAKPAAQFTLEEAQAEVEKLRKQLNQWRLEYYTKDAPTVTDNVYDDHYRDLQALEEAYPKLVTPDSPTQEVGDVINSDF
AKVQHPIPMLSMGDVFSFEELSEWDARMQSNVGHPVDYNVELKIDGLALSLIYENGKLVQGSTRGDGNVGEDVTRNVLTI
ASVPKQLKQPLSLEVRGECYMPKAAFAKLNARQETEGGTPFANPRNAAAGSLRQLDAKVTAARELDTFIYTLIEPEQFNV
TTQHEAIAFMQALGFTTNPSSEVAGDMQAIDTYIKKYTTDRDALPYGIDGIVLKVNDLALQAQLGNTVKVPRWEIAYKFP
PEEAETVIHDIVWTVGRTGVVTPTAVMDPVQLAGTTVARATLHNADMIRDKDIRIGDTVMLHKAGDIIPEVSRVLVAKRP
ADTPPAPIPEVCPSCGQKLVHLDDEVALRCINPMCPAQVQEQLTHFASRNAMNIDGLGPKIVAQLQAKHLVKDVADLYHL
TAADLAKLDKFKEKSINNLLSAINNSRQNSVERLIFGLGIRHVGGKAARILAEHFGDLDHLMTASQEAIADVPNIGPTIG
EAIVTYFKAATVQKLITQLREADVNLRYTGPTKPVVKDSFVAGKTVVITGKFAEFSRPALTKQLEGLGAKVTGSVSKKTD
ILIAGDAAGSKLAKAQSLNVPIMNETELLANLKD
>Mature_674_residues
MLAKPAAQFTLEEAQAEVEKLRKQLNQWRLEYYTKDAPTVTDNVYDDHYRDLQALEEAYPKLVTPDSPTQEVGDVINSDF
AKVQHPIPMLSMGDVFSFEELSEWDARMQSNVGHPVDYNVELKIDGLALSLIYENGKLVQGSTRGDGNVGEDVTRNVLTI
ASVPKQLKQPLSLEVRGECYMPKAAFAKLNARQETEGGTPFANPRNAAAGSLRQLDAKVTAARELDTFIYTLIEPEQFNV
TTQHEAIAFMQALGFTTNPSSEVAGDMQAIDTYIKKYTTDRDALPYGIDGIVLKVNDLALQAQLGNTVKVPRWEIAYKFP
PEEAETVIHDIVWTVGRTGVVTPTAVMDPVQLAGTTVARATLHNADMIRDKDIRIGDTVMLHKAGDIIPEVSRVLVAKRP
ADTPPAPIPEVCPSCGQKLVHLDDEVALRCINPMCPAQVQEQLTHFASRNAMNIDGLGPKIVAQLQAKHLVKDVADLYHL
TAADLAKLDKFKEKSINNLLSAINNSRQNSVERLIFGLGIRHVGGKAARILAEHFGDLDHLMTASQEAIADVPNIGPTIG
EAIVTYFKAATVQKLITQLREADVNLRYTGPTKPVVKDSFVAGKTVVITGKFAEFSRPALTKQLEGLGAKVTGSVSKKTD
ILIAGDAAGSKLAKAQSLNVPIMNETELLANLKD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=41.7287630402384, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI87082305, Length=581, Percent_Identity=22.8915662650602, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_LACC3 (Q03AC3)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806291.1
- ProteinModelPortal:   Q03AC3
- STRING:   Q03AC3
- GeneID:   4418840
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_1055
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q03AC3
- ProtClustDB:   CLSK2532330
- BioCyc:   LCAS321967:LSEI_1055-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73627; Mature: 73627

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 123-123 BINDING 121-121 BINDING 144-144 BINDING 178-178 BINDING 294-294 BINDING 318-318

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKPAAQFTLEEAQAEVEKLRKQLNQWRLEYYTKDAPTVTDNVYDDHYRDLQALEEAYP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
KLVTPDSPTQEVGDVINSDFAKVQHPIPMLSMGDVFSFEELSEWDARMQSNVGHPVDYNV
CCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
ELKIDGLALSLIYENGKLVQGSTRGDGNVGEDVTRNVLTIASVPKQLKQPLSLEVRGECY
EEEECCEEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
MPKAAFAKLNARQETEGGTPFANPRNAAAGSLRQLDAKVTAARELDTFIYTLIEPEQFNV
CCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
TTQHEAIAFMQALGFTTNPSSEVAGDMQAIDTYIKKYTTDRDALPYGIDGIVLKVNDLAL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECEEE
QAQLGNTVKVPRWEIAYKFPPEEAETVIHDIVWTVGRTGVVTPTAVMDPVQLAGTTVARA
EECCCCEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCHHHHHH
TLHNADMIRDKDIRIGDTVMLHKAGDIIPEVSRVLVAKRPADTPPAPIPEVCPSCGQKLV
HHHCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEE
HLDDEVALRCINPMCPAQVQEQLTHFASRNAMNIDGLGPKIVAQLQAKHLVKDVADLYHL
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TAADLAKLDKFKEKSINNLLSAINNSRQNSVERLIFGLGIRHVGGKAARILAEHFGDLDH
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHH
LMTASQEAIADVPNIGPTIGEAIVTYFKAATVQKLITQLREADVNLRYTGPTKPVVKDSF
HHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
VAGKTVVITGKFAEFSRPALTKQLEGLGAKVTGSVSKKTDILIAGDAAGSKLAKAQSLNV
CCCCEEEEEECHHHHCCHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCHHHHHHCCCC
PIMNETELLANLKD
CCCCCHHHHHCCCC
>Mature Secondary Structure
MLAKPAAQFTLEEAQAEVEKLRKQLNQWRLEYYTKDAPTVTDNVYDDHYRDLQALEEAYP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCC
KLVTPDSPTQEVGDVINSDFAKVQHPIPMLSMGDVFSFEELSEWDARMQSNVGHPVDYNV
CCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
ELKIDGLALSLIYENGKLVQGSTRGDGNVGEDVTRNVLTIASVPKQLKQPLSLEVRGECY
EEEECCEEEEEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCC
MPKAAFAKLNARQETEGGTPFANPRNAAAGSLRQLDAKVTAARELDTFIYTLIEPEQFNV
CCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
TTQHEAIAFMQALGFTTNPSSEVAGDMQAIDTYIKKYTTDRDALPYGIDGIVLKVNDLAL
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECEEE
QAQLGNTVKVPRWEIAYKFPPEEAETVIHDIVWTVGRTGVVTPTAVMDPVQLAGTTVARA
EECCCCEECCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCHHHHHH
TLHNADMIRDKDIRIGDTVMLHKAGDIIPEVSRVLVAKRPADTPPAPIPEVCPSCGQKLV
HHHCCHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEE
HLDDEVALRCINPMCPAQVQEQLTHFASRNAMNIDGLGPKIVAQLQAKHLVKDVADLYHL
CCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TAADLAKLDKFKEKSINNLLSAINNSRQNSVERLIFGLGIRHVGGKAARILAEHFGDLDH
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHH
LMTASQEAIADVPNIGPTIGEAIVTYFKAATVQKLITQLREADVNLRYTGPTKPVVKDSF
HHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
VAGKTVVITGKFAEFSRPALTKQLEGLGAKVTGSVSKKTDILIAGDAAGSKLAKAQSLNV
CCCCEEEEEECHHHHCCHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCHHHHHHCCCC
PIMNETELLANLKD
CCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA