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The map label for this gene is pcrA [H]

Identifier: 116494556

GI number: 116494556

Start: 1035001

End: 1037256

Strand: Direct

Name: pcrA [H]

Synonym: LSEI_1054

Alternate gene names: 116494556

Gene position: 1035001-1037256 (Clockwise)

Preceding gene: 116494549

Following gene: 116494557

Centisome position: 35.75

GC content: 46.76

Gene sequence:

>2256_bases
ATGGTAATGGATGCCGCATTAAAGGGTATGAATGATAAGCAATCTGAGGCGGTGTTGGCGACTGAAGGGCCAGTGCTGAT
TATGGCTGGTGCCGGTTCAGGCAAGACACGGGTATTGACTCATCGGATTGCTTATTTAGTTGAAGAAAAGCACGTCAATC
CATGGAACATCCTTGCCATCACGTTTACAAATAAGGCCGCCCGTGAGATGCGCGAGCGAGTCGGCAAGTTGGTTGATGCT
GAGATTGCGCGCGATATTTGGGTCTCAACGTTCCATGCGCTCTGTGTCCGAATTTTACGACGCGACATTGACAAGCTGGG
CTATAACCGTGCCTTTACGATTGCTGATCCTGCTGAGCAACTGACGCTAGTGAAGCATATTTTGGCTGATTTCAATCTTG
ATCCAAAGCGCTACGATCCTAAATCCATTTTAGGTGCTATTTCGAGCGCTAAAAATGAACTCGTGGATCCACAAACTTAT
GCCAATGAACAGGCTAACAGTCCTTTCACCAAAGTTGTGGCGCAGGTTTATGCGGAATATCAAAAGCGTCTGCGCAGTGA
TCAGGCATTGGACTTTGATGATCTCATCATGCAGACCATCGTGCTTTTTGAAAAAGACAAAGAAACATTGGCATTCTACC
AGCGTAAGTTCCGCTACATTCATGTTGACGAATATCAGGACACTAACGAAGCGCAATACCAGCTCGTGCATATGTTGGCA
GAAGGCTACCATAATTTATGTGTGGTTGGTGATGCCGACCAGAGCATTTATGGCTGGCGTGGTGCCAATATGCAGAATAT
TTTGGATTTCAAACAAGACTATCCAAAGGCGCAAGTCATTTTGCTTGAGCAGAATTATCGGTCAACTAAAACTATTTTGA
GTGCGGCCAATGATGTGATTCAGAACAATCAGAATCGCCAAGCCAAAACGTTATGGACGGAAAATCGCGAAGGCGACAAG
ATTACCTATTACCGCGCTCAAAGCGAAACTGATGAAGCTATTTTTGTCATTCGCCGAATTGAAGCAGAGATCGCTGATAA
TCATCGCAAATATGGTGATTTTGCGATTTTGTACCGAACCAATGCGCAGTCGCGGGCGATTGAAGAAGCGTTTGTGAAGT
CCAATATTCCATATAAAATGGTTGGCGGGCATAAGTTCTATGATCGCAAGGAAATTCGCGATGCGCTAGCGTATTTCCGA
TTAGTGGTCAATCCGGAAGACGATATGAGTTTTAATCGAATTGTCAATGAACCAAAACGCGGTATTGGCAACACAAGCGT
TGAGAAGTTAGAGGCCTTTGCCAATGAGCATGACTGGTCCTTGCTGGAAGCAGCGGCGAATGCTGAACTCAGTCCGATTG
CTGGTAAAGCTCGCGGACAGTTAGCTAAGTTCGGTAAAATGATCCAGCAGCTGGGTACACGGCGAAAAGATCTAAACGTG
ACCAAGATGATGCAGGCAATTCTTGAGGATAGTGGCTATTTATCAACACTGCGAGCGGCTCACAATCTGGAAGCCGATAC
CCGCATCGAAAACTTAGAAGAATTGCTTTCGGTGACCCAAGGATTCGATTCCCGGTATCAGCCGGAAAATGAAGACAGCG
ACATTTTCGTTGATTTTCTGGCTGAACTGGCGCTGTTATCGGATCAGGATGACGTTGAAGAAGATGCACAAGAAGTTACC
TTGATGACCTTGCACGCTGCTAAAGGACTGGAATTTCCGGTTGTCTTTTTAGTGGGGATGGAAGAAGGACTCTTTCCATT
AAGTCGGGCTTCCATGGATGAAAGCGAGCTCGAAGAGGAACGGCGCTTAGCTTATGTCGGCATTACCCGCGCCAAACAGA
AGCTGTATCTGTCCAATGCCTATGGCCGGATGCTGTATGGTCAACGGCAAAACAATCCTGCTAGCCGTTTCATTGAAGAA
ATTAATCCTGACTTGTTGGAAGTCATTGGCACTACCCAGCCAAAAAGTGACATTCCATTTATTAATCGTACTGATCGCGC
ATTCAGCTCGCCGTATAGCCGCCACGCTGCTCAAAAAACAGCAACACCCGTTACTGTGCAAGCCGCGCCAACAACAGGTG
CAGGCAAAACCAGTTGGGAAGCTGGCGACAAGGTGAAGCATCGCAAGTGGGGGACTGGTACCGTGGTGAAAGTCACGGGT
ACCGGCGATGATCAAGAACTCGATATTGCTTTTAAAGACAACGGCATCAAACGACTGCTAGCTGCCTTTGCTCCAATTGT
CAAAGTGGCATCATAA

Upstream 100 bases:

>100_bases
AGAGGTCATGATTGACCACTCGCTCACGCTCCCTCCCAAAATTTGGTACAATAGGTCAAGGAATTTTTCACTTGGTTTTT
CTGAAAGGACGATTCGTTAA

Downstream 100 bases:

>100_bases
GCGTTCGCAGTTTGACTCAGAAATGTCGGGATGCATATTTTTAGAGGAGGCTCGCTGTGTTAGCAAAACCAGCTGCACAA
TTCACACTTGAAGAAGCTCA

Product: superfamily I DNA/RNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MVMDAALKGMNDKQSEAVLATEGPVLIMAGAGSGKTRVLTHRIAYLVEEKHVNPWNILAITFTNKAAREMRERVGKLVDA
EIARDIWVSTFHALCVRILRRDIDKLGYNRAFTIADPAEQLTLVKHILADFNLDPKRYDPKSILGAISSAKNELVDPQTY
ANEQANSPFTKVVAQVYAEYQKRLRSDQALDFDDLIMQTIVLFEKDKETLAFYQRKFRYIHVDEYQDTNEAQYQLVHMLA
EGYHNLCVVGDADQSIYGWRGANMQNILDFKQDYPKAQVILLEQNYRSTKTILSAANDVIQNNQNRQAKTLWTENREGDK
ITYYRAQSETDEAIFVIRRIEAEIADNHRKYGDFAILYRTNAQSRAIEEAFVKSNIPYKMVGGHKFYDRKEIRDALAYFR
LVVNPEDDMSFNRIVNEPKRGIGNTSVEKLEAFANEHDWSLLEAAANAELSPIAGKARGQLAKFGKMIQQLGTRRKDLNV
TKMMQAILEDSGYLSTLRAAHNLEADTRIENLEELLSVTQGFDSRYQPENEDSDIFVDFLAELALLSDQDDVEEDAQEVT
LMTLHAAKGLEFPVVFLVGMEEGLFPLSRASMDESELEEERRLAYVGITRAKQKLYLSNAYGRMLYGQRQNNPASRFIEE
INPDLLEVIGTTQPKSDIPFINRTDRAFSSPYSRHAAQKTATPVTVQAAPTTGAGKTSWEAGDKVKHRKWGTGTVVKVTG
TGDDQELDIAFKDNGIKRLLAAFAPIVKVAS

Sequences:

>Translated_751_residues
MVMDAALKGMNDKQSEAVLATEGPVLIMAGAGSGKTRVLTHRIAYLVEEKHVNPWNILAITFTNKAAREMRERVGKLVDA
EIARDIWVSTFHALCVRILRRDIDKLGYNRAFTIADPAEQLTLVKHILADFNLDPKRYDPKSILGAISSAKNELVDPQTY
ANEQANSPFTKVVAQVYAEYQKRLRSDQALDFDDLIMQTIVLFEKDKETLAFYQRKFRYIHVDEYQDTNEAQYQLVHMLA
EGYHNLCVVGDADQSIYGWRGANMQNILDFKQDYPKAQVILLEQNYRSTKTILSAANDVIQNNQNRQAKTLWTENREGDK
ITYYRAQSETDEAIFVIRRIEAEIADNHRKYGDFAILYRTNAQSRAIEEAFVKSNIPYKMVGGHKFYDRKEIRDALAYFR
LVVNPEDDMSFNRIVNEPKRGIGNTSVEKLEAFANEHDWSLLEAAANAELSPIAGKARGQLAKFGKMIQQLGTRRKDLNV
TKMMQAILEDSGYLSTLRAAHNLEADTRIENLEELLSVTQGFDSRYQPENEDSDIFVDFLAELALLSDQDDVEEDAQEVT
LMTLHAAKGLEFPVVFLVGMEEGLFPLSRASMDESELEEERRLAYVGITRAKQKLYLSNAYGRMLYGQRQNNPASRFIEE
INPDLLEVIGTTQPKSDIPFINRTDRAFSSPYSRHAAQKTATPVTVQAAPTTGAGKTSWEAGDKVKHRKWGTGTVVKVTG
TGDDQELDIAFKDNGIKRLLAAFAPIVKVAS
>Mature_751_residues
MVMDAALKGMNDKQSEAVLATEGPVLIMAGAGSGKTRVLTHRIAYLVEEKHVNPWNILAITFTNKAAREMRERVGKLVDA
EIARDIWVSTFHALCVRILRRDIDKLGYNRAFTIADPAEQLTLVKHILADFNLDPKRYDPKSILGAISSAKNELVDPQTY
ANEQANSPFTKVVAQVYAEYQKRLRSDQALDFDDLIMQTIVLFEKDKETLAFYQRKFRYIHVDEYQDTNEAQYQLVHMLA
EGYHNLCVVGDADQSIYGWRGANMQNILDFKQDYPKAQVILLEQNYRSTKTILSAANDVIQNNQNRQAKTLWTENREGDK
ITYYRAQSETDEAIFVIRRIEAEIADNHRKYGDFAILYRTNAQSRAIEEAFVKSNIPYKMVGGHKFYDRKEIRDALAYFR
LVVNPEDDMSFNRIVNEPKRGIGNTSVEKLEAFANEHDWSLLEAAANAELSPIAGKARGQLAKFGKMIQQLGTRRKDLNV
TKMMQAILEDSGYLSTLRAAHNLEADTRIENLEELLSVTQGFDSRYQPENEDSDIFVDFLAELALLSDQDDVEEDAQEVT
LMTLHAAKGLEFPVVFLVGMEEGLFPLSRASMDESELEEERRLAYVGITRAKQKLYLSNAYGRMLYGQRQNNPASRFIEE
INPDLLEVIGTTQPKSDIPFINRTDRAFSSPYSRHAAQKTATPVTVQAAPTTGAGKTSWEAGDKVKHRKWGTGTVVKVTG
TGDDQELDIAFKDNGIKRLLAAFAPIVKVAS

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=750, Percent_Identity=41.3333333333333, Blast_Score=540, Evalue=1e-154,
Organism=Escherichia coli, GI48994965, Length=642, Percent_Identity=39.5638629283489, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1787196, Length=348, Percent_Identity=28.1609195402299, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=729, Percent_Identity=29.4924554183813, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6324477, Length=680, Percent_Identity=25.2941176470588, Blast_Score=134, Evalue=4e-32,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84918; Mature: 84918

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMDAALKGMNDKQSEAVLATEGPVLIMAGAGSGKTRVLTHRIAYLVEEKHVNPWNILAI
CCCCHHHCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAREMRERVGKLVDAEIARDIWVSTFHALCVRILRRDIDKLGYNRAFTIADPAEQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
LTLVKHILADFNLDPKRYDPKSILGAISSAKNELVDPQTYANEQANSPFTKVVAQVYAEY
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHH
QKRLRSDQALDFDDLIMQTIVLFEKDKETLAFYQRKFRYIHVDEYQDTNEAQYQLVHMLA
HHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHH
EGYHNLCVVGDADQSIYGWRGANMQNILDFKQDYPKAQVILLEQNYRSTKTILSAANDVI
HCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
QNNQNRQAKTLWTENREGDKITYYRAQSETDEAIFVIRRIEAEIADNHRKYGDFAILYRT
HCCCCCCCHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NAQSRAIEEAFVKSNIPYKMVGGHKFYDRKEIRDALAYFRLVVNPEDDMSFNRIVNEPKR
CCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHCCHHC
GIGNTSVEKLEAFANEHDWSLLEAAANAELSPIAGKARGQLAKFGKMIQQLGTRRKDLNV
CCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
TKMMQAILEDSGYLSTLRAAHNLEADTRIENLEELLSVTQGFDSRYQPENEDSDIFVDFL
HHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
AELALLSDQDDVEEDAQEVTLMTLHAAKGLEFPVVFLVGMEEGLFPLSRASMDESELEEE
HHHHHHCCCCHHHHHHHHEEHEEEEHHCCCCCCEEEEEECCCCCCCCHHCCCCHHHHHHH
RRLAYVGITRAKQKLYLSNAYGRMLYGQRQNNPASRFIEEINPDLLEVIGTTQPKSDIPF
HCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCC
INRTDRAFSSPYSRHAAQKTATPVTVQAAPTTGAGKTSWEAGDKVKHRKWGTGTVVKVTG
CCCCCHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
TGDDQELDIAFKDNGIKRLLAAFAPIVKVAS
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVMDAALKGMNDKQSEAVLATEGPVLIMAGAGSGKTRVLTHRIAYLVEEKHVNPWNILAI
CCCCHHHCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAREMRERVGKLVDAEIARDIWVSTFHALCVRILRRDIDKLGYNRAFTIADPAEQ
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHH
LTLVKHILADFNLDPKRYDPKSILGAISSAKNELVDPQTYANEQANSPFTKVVAQVYAEY
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHH
QKRLRSDQALDFDDLIMQTIVLFEKDKETLAFYQRKFRYIHVDEYQDTNEAQYQLVHMLA
HHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHH
EGYHNLCVVGDADQSIYGWRGANMQNILDFKQDYPKAQVILLEQNYRSTKTILSAANDVI
HCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
QNNQNRQAKTLWTENREGDKITYYRAQSETDEAIFVIRRIEAEIADNHRKYGDFAILYRT
HCCCCCCCHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
NAQSRAIEEAFVKSNIPYKMVGGHKFYDRKEIRDALAYFRLVVNPEDDMSFNRIVNEPKR
CCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCHHHHHHCCHHC
GIGNTSVEKLEAFANEHDWSLLEAAANAELSPIAGKARGQLAKFGKMIQQLGTRRKDLNV
CCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
TKMMQAILEDSGYLSTLRAAHNLEADTRIENLEELLSVTQGFDSRYQPENEDSDIFVDFL
HHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
AELALLSDQDDVEEDAQEVTLMTLHAAKGLEFPVVFLVGMEEGLFPLSRASMDESELEEE
HHHHHHCCCCHHHHHHHHEEHEEEEHHCCCCCCEEEEEECCCCCCCCHHCCCCHHHHHHH
RRLAYVGITRAKQKLYLSNAYGRMLYGQRQNNPASRFIEEINPDLLEVIGTTQPKSDIPF
HCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCC
INRTDRAFSSPYSRHAAQKTATPVTVQAAPTTGAGKTSWEAGDKVKHRKWGTGTVVKVTG
CCCCCHHHCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEC
TGDDQELDIAFKDNGIKRLLAAFAPIVKVAS
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA