Click here to switch to the map view.

The map label for this gene is tpiA

Identifier: 116494475

GI number: 116494475

Start: 951362

End: 952117

Strand: Direct

Name: tpiA

Synonym: LSEI_0969

Alternate gene names: 116494475

Gene position: 951362-952117 (Clockwise)

Preceding gene: 116494474

Following gene: 116494476

Centisome position: 32.86

GC content: 46.83

Gene sequence:

>756_bases
ATGCGTACACCATTCATTGCTGGTAACTGGAAAATGAACAAAAATCCTAAGGAAACGCAAGAATTCTTAGACGGCGTTAA
AGGCAAGTTGCCTGATGCAAGCAAGGTTGAGACTGTCATTGGCGCACCTGCCATTGATTTGACCACCTTAGTTGCTGGCG
CTGAAGGCACACCTTTGAAGACGGCGGCTGAAAACTGCTACTTTGAAGACGAAGGTGCCTTCACTGGTGAAACCAGCCCG
AAAGCTTTGAAAGAAATGAACGTTGATTACGTCATTATCGGTCACAGCGAACGTCGCGGCTATTTCCACGAAACCGATGA
AGATATCAATAAGAAGGCTAAGGCCATCTTTAAGAACAATCTTTTGCCAATCATTTGCTGCGGCGAAAGTTTGGCTCAGC
GCGAAGCTGGCCAAACCGAAGACTGGGTTGCTTCACAGATTGAAGCTGCTTTGGCTGGTTTAAGTGCTGACCAAGTCAAA
GTTTCAGTCTTAGCCTATGAACCAATCTGGGCTATCGGGACAGGTAAGACGGCAACTGCCGATCAGGCACAAGAAGTTGT
CGCACATATTCGTGCAACGGTTGAGAAGTTGTATAATAAAGATACGGCAGATGCTGTTCGTATTCTTTACGGCGGCTCTG
TTAAACCAGCGAACGTCAAGGAATTAATGGCTAAGCCTGATATCGATGGCGGCTTAGTCGGTGGCGCTTCGATGGATCCT
GAAAGTTTCATCGCCTTGGCTAACTACCAAGATTAA

Upstream 100 bases:

>100_bases
GTATCGCAGCTATTTCTGACAAGTAATTGTCAGGCATGCTGATCTAACAGTCACTACCGGCAGACGGTGTCTGTCGGTAC
ATATGAAAGGAAGATATGCC

Downstream 100 bases:

>100_bases
TCTGGGTTCTACTATTAAACAGCCTTCTGGCAAAAAGGAGAAGAATTTATGTCTATCATTACTGATGTATTGGCACGCGA
AGTCTTAGACTCCCGTGGCA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRTPFIAGNWKMNKNPKETQEFLDGVKGKLPDASKVETVIGAPAIDLTTLVAGAEGTPLKTAAENCYFEDEGAFTGETSP
KALKEMNVDYVIIGHSERRGYFHETDEDINKKAKAIFKNNLLPIICCGESLAQREAGQTEDWVASQIEAALAGLSADQVK
VSVLAYEPIWAIGTGKTATADQAQEVVAHIRATVEKLYNKDTADAVRILYGGSVKPANVKELMAKPDIDGGLVGGASMDP
ESFIALANYQD

Sequences:

>Translated_251_residues
MRTPFIAGNWKMNKNPKETQEFLDGVKGKLPDASKVETVIGAPAIDLTTLVAGAEGTPLKTAAENCYFEDEGAFTGETSP
KALKEMNVDYVIIGHSERRGYFHETDEDINKKAKAIFKNNLLPIICCGESLAQREAGQTEDWVASQIEAALAGLSADQVK
VSVLAYEPIWAIGTGKTATADQAQEVVAHIRATVEKLYNKDTADAVRILYGGSVKPANVKELMAKPDIDGGLVGGASMDP
ESFIALANYQD
>Mature_251_residues
MRTPFIAGNWKMNKNPKETQEFLDGVKGKLPDASKVETVIGAPAIDLTTLVAGAEGTPLKTAAENCYFEDEGAFTGETSP
KALKEMNVDYVIIGHSERRGYFHETDEDINKKAKAIFKNNLLPIICCGESLAQREAGQTEDWVASQIEAALAGLSADQVK
VSVLAYEPIWAIGTGKTATADQAQEVVAHIRATVEKLYNKDTADAVRILYGGSVKPANVKELMAKPDIDGGLVGGASMDP
ESFIALANYQD

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=43.1451612903226, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=43.1451612903226, Blast_Score=176, Evalue=1e-44,
Organism=Escherichia coli, GI1790353, Length=243, Percent_Identity=43.2098765432099, Blast_Score=198, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17536593, Length=251, Percent_Identity=43.8247011952191, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=251, Percent_Identity=39.8406374501992, Blast_Score=170, Evalue=2e-43,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=46.9635627530364, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=46.9635627530364, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=46.9635627530364, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_LACC3 (Q03AK5)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806209.1
- ProteinModelPortal:   Q03AK5
- SMR:   Q03AK5
- STRING:   Q03AK5
- GeneID:   4419210
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0969
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   NCWVRKG
- PhylomeDB:   Q03AK5
- ProtClustDB:   PRK00042
- BioCyc:   LCAS321967:LSEI_0969-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26980; Mature: 26980

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPFIAGNWKMNKNPKETQEFLDGVKGKLPDASKVETVIGAPAIDLTTLVAGAEGTPLK
CCCCEECCCCEECCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCH
TAAENCYFEDEGAFTGETSPKALKEMNVDYVIIGHSERRGYFHETDEDINKKAKAIFKNN
HHHHHCEECCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
LLPIICCGESLAQREAGQTEDWVASQIEAALAGLSADQVKVSVLAYEPIWAIGTGKTATA
CEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEECCCCCCCH
DQAQEVVAHIRATVEKLYNKDTADAVRILYGGSVKPANVKELMAKPDIDGGLVGGASMDP
HHHHHHHHHHHHHHHHHHCCCHHHHEEEEECCCCCCCCHHHHHHCCCCCCCEECCCCCCH
ESFIALANYQD
HHEEEECCCCC
>Mature Secondary Structure
MRTPFIAGNWKMNKNPKETQEFLDGVKGKLPDASKVETVIGAPAIDLTTLVAGAEGTPLK
CCCCEECCCCEECCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCCH
TAAENCYFEDEGAFTGETSPKALKEMNVDYVIIGHSERRGYFHETDEDINKKAKAIFKNN
HHHHHCEECCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
LLPIICCGESLAQREAGQTEDWVASQIEAALAGLSADQVKVSVLAYEPIWAIGTGKTATA
CEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEECCCCCCCH
DQAQEVVAHIRATVEKLYNKDTADAVRILYGGSVKPANVKELMAKPDIDGGLVGGASMDP
HHHHHHHHHHHHHHHHHHCCCHHHHEEEEECCCCCCCCHHHHHHCCCCCCCEECCCCCCH
ESFIALANYQD
HHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA