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The map label for this gene is eno

Identifier: 116494476

GI number: 116494476

Start: 952166

End: 953470

Strand: Direct

Name: eno

Synonym: LSEI_0970

Alternate gene names: 116494476

Gene position: 952166-953470 (Clockwise)

Preceding gene: 116494475

Following gene: 116494477

Centisome position: 32.89

GC content: 48.81

Gene sequence:

>1305_bases
ATGTCTATCATTACTGATGTATTGGCACGCGAAGTCTTAGACTCCCGTGGCAACCCTACTGTTGAAGTTGAATTGTACAC
TGAAGACGGCGGCTTTGGCCGTGCGTTGGTACCATCAGGTGCTTCAACCGGTGAACACGAAGCCGTTGAACTTCGTGATG
GTGACAAGGATCGTTTTGGCGGCAAGGGTGTTTTGAAGGCCGTTGGTCATGTGAACAACGAAATCGCTAAGGCGGTTATT
GGCCTTGACGTGACTGAACAACGCCTAATTGACCAAACCATGATTGACCTTGACGGTACCCCGAACAAGGGCAAGTTTGG
CGCCAATGCTATCTTGGGTGTTTCCTTGGCTGCAGCCCGTGCTGCTGCTGATGAAGTTGGCCTGCCATTGTATCAATATC
TTGGCGGCCCGAATGCCCACGTTCTGCCAACGCCAATGATGAACGTCCTCAATGGTGGTGCACACTCAACCAACACCGTT
GACTTCCAGGAATTCATGATCATGCCTGTTGGCGCTAAGAGCGTTCGTGAAGCTGTTCGGATGGGTTCAGAAACCTTCCA
TGCTTTACAGGCACTGTTGAAGAGTAAAGGCGACATCACCGCTGTTGGTGATGAAGGCGGCTTTGCCCCGAACTTGAAGG
ATAACGAAGAAGCCTTCGAACTTCTTGTTGAAGCAATCAAGAAGGCTGGCTACAAGCCAGGCGACGACATTGCTTTGGCC
TTTGACGTTGCTGCTTCAGAAATGTACGATGCCGAGAGCAAGACATACACAACCAAGTGGTCTAACCCTGACAAGAAGTA
CACCACTGAAGAATGGACCGACATGATTGATGGTTACATTAACAAGTACCCAATCGTTTCTGTTGAAGATCCTATCGACG
AAAACGACTGGGAAGGCTGGCAGACATTCACCAAGAAGATGGGCGACAAAGTCCAAATCGTTGGTGATGATCTGTTTGTT
ACCAACACCGACTACCTGAAGAAGGGTATCGATATGGGTGTTGCTAACTCCATCCTGATCAAGCTGAACCAGATCGGTAC
ATTGACCGAAACCTTCGAAGCCATCGAAATGGCTAAAGAAGCTGGTTACACAGCTGTTGTTTCACATCGTTCCGGTGAAA
CTGAAGATACAACGATTGCTGACTTGGTTGTTGCAACCAACGCTGGCCAGATCAAGACTGGTTCAATGAGCCGGACAGAT
CGTATCGCTAAGTACAACCAGTTAATGCGGATCGAAGATCAACTGGGTGCACAATCCTTGTACAAGGGCCGCAAGTCCTT
CTACAATGTGAAAGCAATCGACTAA

Upstream 100 bases:

>100_bases
CGCTTCGATGGATCCTGAAAGTTTCATCGCCTTGGCTAACTACCAAGATTAATCTGGGTTCTACTATTAAACAGCCTTCT
GGCAAAAAGGAGAAGAATTT

Downstream 100 bases:

>100_bases
TTTGCTTAATTAGTTGGCAGCTTGGAAAGCACGTTTCCGTTAAGGAGCGTGCTTTTTTTGTGGAGCGTGAGCTGGCGCGG
TTAGAAACCAGAGTGTGTAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSIITDVLAREVLDSRGNPTVEVELYTEDGGFGRALVPSGASTGEHEAVELRDGDKDRFGGKGVLKAVGHVNNEIAKAVI
GLDVTEQRLIDQTMIDLDGTPNKGKFGANAILGVSLAAARAAADEVGLPLYQYLGGPNAHVLPTPMMNVLNGGAHSTNTV
DFQEFMIMPVGAKSVREAVRMGSETFHALQALLKSKGDITAVGDEGGFAPNLKDNEEAFELLVEAIKKAGYKPGDDIALA
FDVAASEMYDAESKTYTTKWSNPDKKYTTEEWTDMIDGYINKYPIVSVEDPIDENDWEGWQTFTKKMGDKVQIVGDDLFV
TNTDYLKKGIDMGVANSILIKLNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTD
RIAKYNQLMRIEDQLGAQSLYKGRKSFYNVKAID

Sequences:

>Translated_434_residues
MSIITDVLAREVLDSRGNPTVEVELYTEDGGFGRALVPSGASTGEHEAVELRDGDKDRFGGKGVLKAVGHVNNEIAKAVI
GLDVTEQRLIDQTMIDLDGTPNKGKFGANAILGVSLAAARAAADEVGLPLYQYLGGPNAHVLPTPMMNVLNGGAHSTNTV
DFQEFMIMPVGAKSVREAVRMGSETFHALQALLKSKGDITAVGDEGGFAPNLKDNEEAFELLVEAIKKAGYKPGDDIALA
FDVAASEMYDAESKTYTTKWSNPDKKYTTEEWTDMIDGYINKYPIVSVEDPIDENDWEGWQTFTKKMGDKVQIVGDDLFV
TNTDYLKKGIDMGVANSILIKLNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTD
RIAKYNQLMRIEDQLGAQSLYKGRKSFYNVKAID
>Mature_433_residues
SIITDVLAREVLDSRGNPTVEVELYTEDGGFGRALVPSGASTGEHEAVELRDGDKDRFGGKGVLKAVGHVNNEIAKAVIG
LDVTEQRLIDQTMIDLDGTPNKGKFGANAILGVSLAAARAAADEVGLPLYQYLGGPNAHVLPTPMMNVLNGGAHSTNTVD
FQEFMIMPVGAKSVREAVRMGSETFHALQALLKSKGDITAVGDEGGFAPNLKDNEEAFELLVEAIKKAGYKPGDDIALAF
DVAASEMYDAESKTYTTKWSNPDKKYTTEEWTDMIDGYINKYPIVSVEDPIDENDWEGWQTFTKKMGDKVQIVGDDLFVT
NTDYLKKGIDMGVANSILIKLNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDR
IAKYNQLMRIEDQLGAQSLYKGRKSFYNVKAID

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=52.9953917050691, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI4503571, Length=439, Percent_Identity=52.1640091116173, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=52.1739130434783, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=52.1739130434783, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI301897479, Length=438, Percent_Identity=47.2602739726027, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI169201331, Length=341, Percent_Identity=24.3401759530792, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI169201757, Length=341, Percent_Identity=24.3401759530792, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI239744207, Length=341, Percent_Identity=24.3401759530792, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=61.7715617715618, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=53.3180778032037, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=53.3180778032037, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=45.1776649746193, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.1154734411085, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.1154734411085, Blast_Score=398, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.8845265588915, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=50.5773672055427, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=50.8083140877598, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=439, Percent_Identity=50.7972665148064, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580916, Length=439, Percent_Identity=50.7972665148064, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580920, Length=439, Percent_Identity=50.7972665148064, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580914, Length=439, Percent_Identity=50.7972665148064, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281360527, Length=439, Percent_Identity=50.7972665148064, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=439, Percent_Identity=50.7972665148064, Blast_Score=397, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_LACC3 (Q03AK4)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806210.1
- ProteinModelPortal:   Q03AK4
- SMR:   Q03AK4
- STRING:   Q03AK4
- GeneID:   4419211
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0970
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q03AK4
- ProtClustDB:   PRK00077
- BioCyc:   LCAS321967:LSEI_0970-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47087; Mature: 46956

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 341-341 BINDING 155-155 BINDING 164-164 BINDING 289-289 BINDING 316-316 BINDING 341-341 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIITDVLAREVLDSRGNPTVEVELYTEDGGFGRALVPSGASTGEHEAVELRDGDKDRFG
CHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVGHVNNEIAKAVIGLDVTEQRLIDQTMIDLDGTPNKGKFGANAILGVSLAAAR
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
AAADEVGLPLYQYLGGPNAHVLPTPMMNVLNGGAHSTNTVDFQEFMIMPVGAKSVREAVR
HHHHHCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHEEECCCHHHHHHHHH
MGSETFHALQALLKSKGDITAVGDEGGFAPNLKDNEEAFELLVEAIKKAGYKPGDDIALA
CCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
FDVAASEMYDAESKTYTTKWSNPDKKYTTEEWTDMIDGYINKYPIVSVEDPIDENDWEGW
EEHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
QTFTKKMGDKVQIVGDDLFVTNTDYLKKGIDMGVANSILIKLNQIGTLTETFEAIEMAKE
HHHHHHCCCEEEEEECCEEEECHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
AGYTAVVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDRIAKYNQLMRIEDQLGAQSL
CCCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHCHHHH
YKGRKSFYNVKAID
HHHHHHHCEEEECC
>Mature Secondary Structure 
SIITDVLAREVLDSRGNPTVEVELYTEDGGFGRALVPSGASTGEHEAVELRDGDKDRFG
HHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVGHVNNEIAKAVIGLDVTEQRLIDQTMIDLDGTPNKGKFGANAILGVSLAAAR
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
AAADEVGLPLYQYLGGPNAHVLPTPMMNVLNGGAHSTNTVDFQEFMIMPVGAKSVREAVR
HHHHHCCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCEEHHHHEEECCCHHHHHHHHH
MGSETFHALQALLKSKGDITAVGDEGGFAPNLKDNEEAFELLVEAIKKAGYKPGDDIALA
CCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
FDVAASEMYDAESKTYTTKWSNPDKKYTTEEWTDMIDGYINKYPIVSVEDPIDENDWEGW
EEHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHH
QTFTKKMGDKVQIVGDDLFVTNTDYLKKGIDMGVANSILIKLNQIGTLTETFEAIEMAKE
HHHHHHCCCEEEEEECCEEEECHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
AGYTAVVSHRSGETEDTTIADLVVATNAGQIKTGSMSRTDRIAKYNQLMRIEDQLGAQSL
CCCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHCHHHH
YKGRKSFYNVKAID
HHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA