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The map label for this gene is prfB [H]

Identifier: 116494437

GI number: 116494437

Start: 909064

End: 910170

Strand: Direct

Name: prfB [H]

Synonym: LSEI_0930

Alternate gene names: 116494437

Gene position: 909064-910170 (Clockwise)

Preceding gene: 116494436

Following gene: 116494438

Centisome position: 31.4

GC content: 47.79

Gene sequence:

>1107_bases
GTGTTATGCGCAACACCTTGGCCGCCATGCGGACCAAAATTAATCAATTTAGGGGGTCACTTTGACCTCGATGCGCTGAA
CGAACGCATTACTGAAAATGAAGGCCGAATGGCTGAACCCGGCTTTTGGGATGACTCGGAGGCTGCTCAAAAGGTTATTG
ACAACAACAATGCGCTCAAAGAAAAGCATGATCGATTTTATGCACTAGAAAATGAGTTGGAAGATCTTGAGGCTGGTTTT
GAGTTATTACAAGACGAGCCTGATAAGGACTTGCAGCAAGAAGAAGAAGCAAAGATCAAAGATTTACAGGAAAAGCTGGA
TAAGTATGAGATGCAGCTTTTGCTGAACGGCCCATACGATCACAACAATGCCATTTTGGAAATTCACCCTGGTGCAGGCG
GCACGGAATCTCAGGATTGGGGATCAATGCTGATGCGGATGTATCAGCGTTGGGCCGATCAGAACGGATTCAAAGTAGAA
GTGGCGGATTATCAGCCTGGGGATGAAGCTGGCATCAAGAGCGTAACGCTGCTGATCAATGGCGAGAATGCTTATGGCTT
GCTGAAAAGTGAAAAAGGCGTCCATCGACTTGTGCGGATTTCGCCATTTGATTCCGCTGGACGGCGACACACTAGCTTTG
CCTCAGTGGACGTGATGCCGGAACTCGACAACTCAATCGAAGTTGATCTGCGGCCGGAAGACATCAAAATGGAGGTTTTC
CGCTCAAGCGGCGCTGGCGGTCAGCATATCAACAAAACCTCCAGTGCGGTTCGCTTGATTCATCTACCAACCGGAATCGT
CACCTCAAGTCAGGCGCAACGGTCACAGTTTCAGAACAAAGCGACTGCGATGGCGATGTTAAAGGCTAAATTGTATCAGC
GCGAAGAAGAAGCTAAGCGTGCTAAGGCCAAGGCCATTCAAGGCGAACAAAAAGATGTCGCATTTGGTTCACAAATTCGC
TCTTACGTCTTTCACCCATACACGATGGTGAAGGATCATCGCACGAATTATGAAACCGGCAATGGCAATGCGGTTATGGA
CGGGGATTTGAATCCATTCATCAATGCGTATCTACAATGGGAACTTGCGCAAAAGAATCCTGATTAA

Upstream 100 bases:

>100_bases
CTTGCGGTACTGGGGTGTTTTGAAGCAGTCGCCGACGCACATAGTCATGCTACAATAACGCAACTATGTTTAAAAAAGGG
AGATTACACGATGGAACTGA

Downstream 100 bases:

>100_bases
ACTGTAATCTGCCTGAAACCTTGCTGACTTAATCGCCTGTTATACTGATGCTAGCTTAGAAAAAGGAGCAATTTGCATGA
TTCAAATGGAGAACGTGACG

Product: protein chain release factor B

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MLCATPWPPCGPKLINLGGHFDLDALNERITENEGRMAEPGFWDDSEAAQKVIDNNNALKEKHDRFYALENELEDLEAGF
ELLQDEPDKDLQQEEEAKIKDLQEKLDKYEMQLLLNGPYDHNNAILEIHPGAGGTESQDWGSMLMRMYQRWADQNGFKVE
VADYQPGDEAGIKSVTLLINGENAYGLLKSEKGVHRLVRISPFDSAGRRHTSFASVDVMPELDNSIEVDLRPEDIKMEVF
RSSGAGGQHINKTSSAVRLIHLPTGIVTSSQAQRSQFQNKATAMAMLKAKLYQREEEAKRAKAKAIQGEQKDVAFGSQIR
SYVFHPYTMVKDHRTNYETGNGNAVMDGDLNPFINAYLQWELAQKNPD

Sequences:

>Translated_368_residues
MLCATPWPPCGPKLINLGGHFDLDALNERITENEGRMAEPGFWDDSEAAQKVIDNNNALKEKHDRFYALENELEDLEAGF
ELLQDEPDKDLQQEEEAKIKDLQEKLDKYEMQLLLNGPYDHNNAILEIHPGAGGTESQDWGSMLMRMYQRWADQNGFKVE
VADYQPGDEAGIKSVTLLINGENAYGLLKSEKGVHRLVRISPFDSAGRRHTSFASVDVMPELDNSIEVDLRPEDIKMEVF
RSSGAGGQHINKTSSAVRLIHLPTGIVTSSQAQRSQFQNKATAMAMLKAKLYQREEEAKRAKAKAIQGEQKDVAFGSQIR
SYVFHPYTMVKDHRTNYETGNGNAVMDGDLNPFINAYLQWELAQKNPD
>Mature_368_residues
MLCATPWPPCGPKLINLGGHFDLDALNERITENEGRMAEPGFWDDSEAAQKVIDNNNALKEKHDRFYALENELEDLEAGF
ELLQDEPDKDLQQEEEAKIKDLQEKLDKYEMQLLLNGPYDHNNAILEIHPGAGGTESQDWGSMLMRMYQRWADQNGFKVE
VADYQPGDEAGIKSVTLLINGENAYGLLKSEKGVHRLVRISPFDSAGRRHTSFASVDVMPELDNSIEVDLRPEDIKMEVF
RSSGAGGQHINKTSSAVRLIHLPTGIVTSSQAQRSQFQNKATAMAMLKAKLYQREEEAKRAKAKAIQGEQKDVAFGSQIR
SYVFHPYTMVKDHRTNYETGNGNAVMDGDLNPFINAYLQWELAQKNPD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=37.4172185430464, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI34577120, Length=302, Percent_Identity=34.4370860927152, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI166795305, Length=199, Percent_Identity=36.1809045226131, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=46.6472303206997, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI1787462, Length=346, Percent_Identity=36.1271676300578, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=212, Percent_Identity=35.377358490566, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=319, Percent_Identity=31.3479623824451, Blast_Score=144, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19921226, Length=273, Percent_Identity=34.4322344322344, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41444; Mature: 41444

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCATPWPPCGPKLINLGGHFDLDALNERITENEGRMAEPGFWDDSEAAQKVIDNNNALK
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
EKHDRFYALENELEDLEAGFELLQDEPDKDLQQEEEAKIKDLQEKLDKYEMQLLLNGPYD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCC
HNNAILEIHPGAGGTESQDWGSMLMRMYQRWADQNGFKVEVADYQPGDEAGIKSVTLLIN
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEE
GENAYGLLKSEKGVHRLVRISPFDSAGRRHTSFASVDVMPELDNSIEVDLRPEDIKMEVF
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEE
RSSGAGGQHINKTSSAVRLIHLPTGIVTSSQAQRSQFQNKATAMAMLKAKLYQREEEAKR
ECCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAKAIQGEQKDVAFGSQIRSYVFHPYTMVKDHRTNYETGNGNAVMDGDLNPFINAYLQW
HHHHHHCCCCHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCEEECCCCCHHHHHHEEE
ELAQKNPD
EECCCCCC
>Mature Secondary Structure
MLCATPWPPCGPKLINLGGHFDLDALNERITENEGRMAEPGFWDDSEAAQKVIDNNNALK
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
EKHDRFYALENELEDLEAGFELLQDEPDKDLQQEEEAKIKDLQEKLDKYEMQLLLNGPYD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCC
HNNAILEIHPGAGGTESQDWGSMLMRMYQRWADQNGFKVEVADYQPGDEAGIKSVTLLIN
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEE
GENAYGLLKSEKGVHRLVRISPFDSAGRRHTSFASVDVMPELDNSIEVDLRPEDIKMEVF
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCEEEEE
RSSGAGGQHINKTSSAVRLIHLPTGIVTSSQAQRSQFQNKATAMAMLKAKLYQREEEAKR
ECCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAKAIQGEQKDVAFGSQIRSYVFHPYTMVKDHRTNYETGNGNAVMDGDLNPFINAYLQW
HHHHHHCCCCHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCEEECCCCCHHHHHHEEE
ELAQKNPD
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]