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The map label for this gene is recA [H]

Identifier: 116494430

GI number: 116494430

Start: 899917

End: 900978

Strand: Direct

Name: recA [H]

Synonym: LSEI_0923

Alternate gene names: 116494430

Gene position: 899917-900978 (Clockwise)

Preceding gene: 116494429

Following gene: 116494431

Centisome position: 31.08

GC content: 49.44

Gene sequence:

>1062_bases
ATGGCAGGAGACGAACGCCAGAAAGCATTGGATGTTGCACTGAAAAAGATTGAAAAAAATTTTGGTAAAGGCGCGATTAT
GCGAATGGGTGCTAAGGCGGACACGCGCGTTTCCGTTGTCTCTAGCGGCTCACTTGCCGTTGATGATGCACTTGGTGTTG
GGGGCTTTCCGCGCGGACGAATTGTCGAAATTTATGGACCGGAAAGTTCCGGCAAAACAACCGTCGCGCTGCATGCTGTT
GCTGAAGTGCAGAAGCAAGGCGGTACTGCCGCCTATATTGATGCCGAGAATGCAATGGATCCCAAGTACGCAACTGCTTT
GGGCGTCAATATTGACGAATTATTGCTCTCCCAGCCAGACACAGGCGAACAAGGGCTGGAGATTGCTGATGAACTAGTCG
CGTCTGGTGCCATTGATATTGTGGTGATTGATTCAGTTGCTGCTTTGGTGCCGCGGGCTGAGATTGAGGGCGATATGGGG
GATGCCCATGTTGGCTTGCAAGCCAGATTAATGTCACAGGCGTTACGTAAGTTATCCGGTTCCATCAATAAGACAAAAAC
GATTGCTTTATTCATTAATCAGATTCGTGAGAAAGTCGGGATCATTTTTGGGAGTCCGGAAACGACACCAGGTGGTCGTG
CCTTGAAGTTTTATGCGACTGTTCGGTTAGAGATCCGGCGGTCGGAACAGATCAAGACAGGCGCAGATGTCGTCGGCAAT
CGCACTAAAATCAAAGTGGTCAAGAATAAGGTGGCACCGCCTTTTCGAACAGCAATTGTGGATATCATGTATGGCCAAGG
CATTTCGCAAACTGGCGAACTTGTCGACATGGCCGTTGAGAGAGATATTGTTGAAAAAGCAGGTTCATGGTACGCGTATC
AAGGTGAACGCATCGGCCAAGGTCGCGAAAACGCTAAGACATATCTTGACAACCACGCTGATCTTCAGCAGACGATTAAA
CAACAGGTTCGCGTGGCTTATGGTATGGCATCCGCACCTTGTCCGGAGACGGCTTCAGTCAGTGAAGATCAACCCGAAAT
ACCTCAACCGGCTGAAGTATGA

Upstream 100 bases:

>100_bases
TGATGACTGGTATGATGCTTGCATTAAGGGCACTGCAGGCCTAACAGAATCATTCTTTAACACGTTAATACGTCAGGTTT
ACGCAAGAGGAGGCGGCAAG

Downstream 100 bases:

>100_bases
ATCATGAGTCAACTTGCTGTTGTCGGATGTGATGAGTCCGATGGCGCAAGTTTTTTTATTTGAGCGGTCTTAAGGAGATT
TGATATATGTTTTATTTTTC

Product: RecA/RadA recombinase

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MAGDERQKALDVALKKIEKNFGKGAIMRMGAKADTRVSVVSSGSLAVDDALGVGGFPRGRIVEIYGPESSGKTTVALHAV
AEVQKQGGTAAYIDAENAMDPKYATALGVNIDELLLSQPDTGEQGLEIADELVASGAIDIVVIDSVAALVPRAEIEGDMG
DAHVGLQARLMSQALRKLSGSINKTKTIALFINQIREKVGIIFGSPETTPGGRALKFYATVRLEIRRSEQIKTGADVVGN
RTKIKVVKNKVAPPFRTAIVDIMYGQGISQTGELVDMAVERDIVEKAGSWYAYQGERIGQGRENAKTYLDNHADLQQTIK
QQVRVAYGMASAPCPETASVSEDQPEIPQPAEV

Sequences:

>Translated_353_residues
MAGDERQKALDVALKKIEKNFGKGAIMRMGAKADTRVSVVSSGSLAVDDALGVGGFPRGRIVEIYGPESSGKTTVALHAV
AEVQKQGGTAAYIDAENAMDPKYATALGVNIDELLLSQPDTGEQGLEIADELVASGAIDIVVIDSVAALVPRAEIEGDMG
DAHVGLQARLMSQALRKLSGSINKTKTIALFINQIREKVGIIFGSPETTPGGRALKFYATVRLEIRRSEQIKTGADVVGN
RTKIKVVKNKVAPPFRTAIVDIMYGQGISQTGELVDMAVERDIVEKAGSWYAYQGERIGQGRENAKTYLDNHADLQQTIK
QQVRVAYGMASAPCPETASVSEDQPEIPQPAEV
>Mature_352_residues
AGDERQKALDVALKKIEKNFGKGAIMRMGAKADTRVSVVSSGSLAVDDALGVGGFPRGRIVEIYGPESSGKTTVALHAVA
EVQKQGGTAAYIDAENAMDPKYATALGVNIDELLLSQPDTGEQGLEIADELVASGAIDIVVIDSVAALVPRAEIEGDMGD
AHVGLQARLMSQALRKLSGSINKTKTIALFINQIREKVGIIFGSPETTPGGRALKFYATVRLEIRRSEQIKTGADVVGNR
TKIKVVKNKVAPPFRTAIVDIMYGQGISQTGELVDMAVERDIVEKAGSWYAYQGERIGQGRENAKTYLDNHADLQQTIKQ
QVRVAYGMASAPCPETASVSEDQPEIPQPAEV

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=325, Percent_Identity=58.7692307692308, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 37678; Mature: 37547

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGDERQKALDVALKKIEKNFGKGAIMRMGAKADTRVSVVSSGSLAVDDALGVGGFPRGR
CCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEECCCEEECCCCCCCCCCCCC
IVEIYGPESSGKTTVALHAVAEVQKQGGTAAYIDAENAMDPKYATALGVNIDELLLSQPD
EEEEECCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCCCHHHEECCCHHHHHHCCCC
TGEQGLEIADELVASGAIDIVVIDSVAALVPRAEIEGDMGDAHVGLQARLMSQALRKLSG
CCCHHHHHHHHHHHCCCEEEEEECHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
SINKTKTIALFINQIREKVGIIFGSPETTPGGRALKFYATVRLEIRRSEQIKTGADVVGN
CCCCHHHHHHHHHHHHHHHCEEECCCCCCCCCCEEEEEEEEEEEEECCCCHHCCHHHHCC
RTKIKVVKNKVAPPFRTAIVDIMYGQGISQTGELVDMAVERDIVEKAGSWYAYQGERIGQ
CEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCC
GRENAKTYLDNHADLQQTIKQQVRVAYGMASAPCPETASVSEDQPEIPQPAEV
CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGDERQKALDVALKKIEKNFGKGAIMRMGAKADTRVSVVSSGSLAVDDALGVGGFPRGR
CCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEECCCEEECCCCCCCCCCCCC
IVEIYGPESSGKTTVALHAVAEVQKQGGTAAYIDAENAMDPKYATALGVNIDELLLSQPD
EEEEECCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCCCHHHEECCCHHHHHHCCCC
TGEQGLEIADELVASGAIDIVVIDSVAALVPRAEIEGDMGDAHVGLQARLMSQALRKLSG
CCCHHHHHHHHHHHCCCEEEEEECHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
SINKTKTIALFINQIREKVGIIFGSPETTPGGRALKFYATVRLEIRRSEQIKTGADVVGN
CCCCHHHHHHHHHHHHHHHCEEECCCCCCCCCCEEEEEEEEEEEEECCCCHHCCHHHHCC
RTKIKVVKNKVAPPFRTAIVDIMYGQGISQTGELVDMAVERDIVEKAGSWYAYQGERIGQ
CEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCC
GRENAKTYLDNHADLQQTIKQQVRVAYGMASAPCPETASVSEDQPEIPQPAEV
CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA