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The map label for this gene is insI1 [H]

Identifier: 116494415

GI number: 116494415

Start: 883138

End: 884136

Strand: Direct

Name: insI1 [H]

Synonym: LSEI_0907

Alternate gene names: 116494415

Gene position: 883138-884136 (Clockwise)

Preceding gene: 116494411

Following gene: 116494417

Centisome position: 30.5

GC content: 51.25

Gene sequence:

>999_bases
ATGAGTCCGTACACCCATCTTACCTTAAAAGACCGTGAATCGATACTGCTTGGTATCTCTACAGGCAAAACTCTTGATAC
CATCGCCAAAGAGATAGGTCGTTCCAAGAGTACAGTCAGCCGTGAAATTGCACGTAACGGCGGCTGGCGGAGCTATTCGG
CAGCCACCGCTCAGGACCGCTACCGGCGGGTTCGCTTGGCTAGCAGGCGTCCTCGGATCCTCGATCGACCGGGGACTCGT
GACGCTGTCATTCGATATATCACGGTGCTACATTGGTCGCCTGAGCAGATTGCCGGTCGCTTGTCACTAGAAGGCAGTCC
TATTCGCATCAGCTATTCGACTATCTACAGAGGTATCTACCTAGATAATCTCGGCGTTCCATTGAAGAGCCATGGTGCTC
GCGGGCTACCAAGGCTGCTTCGACACCGAGGCAAGACGCGCAAAATCAAAGGCACCATAAATGAACGCCGGGGGCGCTTC
AATGACGTGCCATCAATTCACGACCGACCCCGGTCGGCAGAAAATCGCAGCTGGTTTGGTCACTGGGAAGGCGATACAGT
ACGCGGTAAAACAGGACACTCTGCATTAGTAACATTAGTTGACCGTAAATCACGCTATCTGCTTTCGAAGCGAACGGCCA
ACGCAAAAGCTGACACTGTTAGAGACGTCATGATTGAGCTGCTTGGTGCCTTACCAGCTAACCGAGTAAGAACAGTGACT
CCTGACCGTGGAACGGAGTTTGCCCGGTACAGGGAGCTGGCAGAACGTCTGAATACAAAGGTCTTCTTTCCTGACCCACA
CGCGCCTCAACAACGAGGAACTAACGAAAACACCAACGGACTGATTAGAGAATACTTTCCCAAGAACACAGACCTAGACC
TTCAGAGCGACCAGGAAATTGAGACTTACATTGAACAACTGAATAATCGACCACGCAAGGTCTTAGGCTGGAAGACGTCA
TCAGAAGTCTTCATGGGTAAAAAGTTGCACTTGAGTTGA

Upstream 100 bases:

>100_bases
ACAATTCAAGTGCAACAAACTAGTCAAATAGAAAAGACTCATCGCCTGAGTCCTTGTAAAATGGAATCACCACAAGACCA
ACTACAAGGAGAACTCGACT

Downstream 100 bases:

>100_bases
CAATTCGTCAGTTAAAAAAAGCCCAACCACCAAGCAAAACAGGTCTTGGGGCTGAGCCAATAAAAAGTTGTATCGATGGT
TAAACATAAGACAGACGGTT

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATAQDRYRRVRLASRRPRILDRPGTR
DAVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIYLDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRF
NDVPSIHDRPRSAENRSWFGHWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVT
PDRGTEFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEIETYIEQLNNRPRKVLGWKTS
SEVFMGKKLHLS

Sequences:

>Translated_332_residues
MSPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATAQDRYRRVRLASRRPRILDRPGTR
DAVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIYLDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRF
NDVPSIHDRPRSAENRSWFGHWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVT
PDRGTEFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEIETYIEQLNNRPRKVLGWKTS
SEVFMGKKLHLS
>Mature_331_residues
SPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATAQDRYRRVRLASRRPRILDRPGTRD
AVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIYLDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRFN
DVPSIHDRPRSAENRSWFGHWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVTP
DRGTEFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEIETYIEQLNNRPRKVLGWKTSS
EVFMGKKLHLS

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=333, Percent_Identity=41.4414414414414, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790736, Length=333, Percent_Identity=41.4414414414414, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1787671, Length=333, Percent_Identity=41.4414414414414, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 37964; Mature: 37833

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATAQDR
CCCCCEEEEECCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
YRRVRLASRRPRILDRPGTRDAVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCEEEECCCCEEEEHHHHHCEEE
LDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRFNDVPSIHDRPRSAENRSWFG
ECCCCCCHHCCCCCCHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCEEE
HWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVT
ECCCCEECCCCCCHHEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEC
PDRGTEFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEI
CCCCCHHHHHHHHHHHHCCEEECCCCCCHHHCCCCCCCCCHHHHHCCCCCCCCCCCCHHH
ETYIEQLNNRPRKVLGWKTSSEVFMGKKLHLS
HHHHHHHCCCCCEEECCCCCCHHHCCEEECCC
>Mature Secondary Structure 
SPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATAQDR
CCCCEEEEECCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
YRRVRLASRRPRILDRPGTRDAVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCEEEECCCCEEEEHHHHHCEEE
LDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRFNDVPSIHDRPRSAENRSWFG
ECCCCCCHHCCCCCCHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCEEE
HWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVT
ECCCCEECCCCCCHHEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEC
PDRGTEFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEI
CCCCCHHHHHHHHHHHHCCEEECCCCCCHHHCCCCCCCCCHHHHHCCCCCCCCCCCCHHH
ETYIEQLNNRPRKVLGWKTSSEVFMGKKLHLS
HHHHHHHCCCCCEEECCCCCCHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]