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The map label for this gene is ytzG [H]

Identifier: 116494395

GI number: 116494395

Start: 863402

End: 864112

Strand: Direct

Name: ytzG [H]

Synonym: LSEI_0886

Alternate gene names: 116494395

Gene position: 863402-864112 (Clockwise)

Preceding gene: 116494394

Following gene: 116494396

Centisome position: 29.82

GC content: 48.1

Gene sequence:

>711_bases
ATGCGATTAGATAAATATTTGAGTCATTTACAATTTGGTAGTCGAAAAGAAGTTAAGGCACTTATTCGCGACAAGCGGGT
ACGTGTGGCCGGTGATTTAATCACTGATCCTGGTTACAATGTGTTGCCGGGGATCGCGGTCGAGGTGAACGATGCCCAAG
CGGATGGGCCGCTTGAGGTCGATTATTTGATGAACAAACCGGCTGGGGTGATTACCGCAACAGAAGATCCTACGCAATCA
ACGGTGCTGGATCTGATCCGACCACATGATTACCGACCGGGTTTATATCCCGTAGGTCGTCTGGATAAAGACACTACTGG
TCTGTTGTTACTTACGACCGATGGCAATTTAGGTCATGCACTGCTTTCGCCTAATCGTCATATTGCCAAAACCTATGCTG
CAACTTTGGCTAAGACATTAACTGCTGACATGAAGCAGCGACTCGAAACCGGCATTGATTTAAAGGACTTCACAACCGCC
CCGGCACAAGTCGTTGTCTTGCCGGATACCGATGGCAAACGCATCCAGATTACAATCACGGAAGGTAAGTTTCATCAAGT
CAAGCGCATGTTGTTGGCAGTTGACAATGAGGTGACGGCCTTGACGCGAATTGCTATGGGACCATTGTCATTGCCCGCCG
ATTTAGCTGCTGGGGAGTATCGGGCTTTAACCGATGACGAACGGACTGATTTGGATAATATCACTCGTTGA

Upstream 100 bases:

>100_bases
TTGATTTACGTTTATTTGATTTTGAAAACGCGTTTGGCTGATTTAATCATTGGTGAGCGAATTAGTCGACTTCGCGATAT
TTTACACATTCGGTGAGCTT

Downstream 100 bases:

>100_bases
GCAACCGAGCACTTTGGTGTGGTGATTGTTTGGCTTTGTCTAAGCGAGAGATTTTGTTTGTTACAGGAGTTTAAGAGAGC
GGCCTTGATTAGGCGAACCA

Product: 16S rRNA uridine-516 pseudouridylate synthase related pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MRLDKYLSHLQFGSRKEVKALIRDKRVRVAGDLITDPGYNVLPGIAVEVNDAQADGPLEVDYLMNKPAGVITATEDPTQS
TVLDLIRPHDYRPGLYPVGRLDKDTTGLLLLTTDGNLGHALLSPNRHIAKTYAATLAKTLTADMKQRLETGIDLKDFTTA
PAQVVVLPDTDGKRIQITITEGKFHQVKRMLLAVDNEVTALTRIAMGPLSLPADLAAGEYRALTDDERTDLDNITR

Sequences:

>Translated_236_residues
MRLDKYLSHLQFGSRKEVKALIRDKRVRVAGDLITDPGYNVLPGIAVEVNDAQADGPLEVDYLMNKPAGVITATEDPTQS
TVLDLIRPHDYRPGLYPVGRLDKDTTGLLLLTTDGNLGHALLSPNRHIAKTYAATLAKTLTADMKQRLETGIDLKDFTTA
PAQVVVLPDTDGKRIQITITEGKFHQVKRMLLAVDNEVTALTRIAMGPLSLPADLAAGEYRALTDDERTDLDNITR
>Mature_236_residues
MRLDKYLSHLQFGSRKEVKALIRDKRVRVAGDLITDPGYNVLPGIAVEVNDAQADGPLEVDYLMNKPAGVITATEDPTQS
TVLDLIRPHDYRPGLYPVGRLDKDTTGLLLLTTDGNLGHALLSPNRHIAKTYAATLAKTLTADMKQRLETGIDLKDFTTA
PAQVVVLPDTDGKRIQITITEGKFHQVKRMLLAVDNEVTALTRIAMGPLSLPADLAAGEYRALTDDERTDLDNITR

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=39.3162393162393, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=33.0508474576271, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787524, Length=233, Percent_Identity=29.6137339055794, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=34.0782122905028, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 25895; Mature: 25895

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKYLSHLQFGSRKEVKALIRDKRVRVAGDLITDPGYNVLPGIAVEVNDAQADGPLEV
CCHHHHHHHHCCCCHHHHHHHHHHCCEEEECEEECCCCCCCCCCEEEEECCCCCCCCEEE
DYLMNKPAGVITATEDPTQSTVLDLIRPHDYRPGLYPVGRLDKDTTGLLLLTTDGNLGHA
EEEECCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
LLSPNRHIAKTYAATLAKTLTADMKQRLETGIDLKDFTTAPAQVVVLPDTDGKRIQITIT
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCCCEEEEEEE
EGKFHQVKRMLLAVDNEVTALTRIAMGPLSLPADLAAGEYRALTDDERTDLDNITR
CCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHCCCCEEECCCCCCCCHHHCCC
>Mature Secondary Structure
MRLDKYLSHLQFGSRKEVKALIRDKRVRVAGDLITDPGYNVLPGIAVEVNDAQADGPLEV
CCHHHHHHHHCCCCHHHHHHHHHHCCEEEECEEECCCCCCCCCCEEEEECCCCCCCCEEE
DYLMNKPAGVITATEDPTQSTVLDLIRPHDYRPGLYPVGRLDKDTTGLLLLTTDGNLGHA
EEEECCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
LLSPNRHIAKTYAATLAKTLTADMKQRLETGIDLKDFTTAPAQVVVLPDTDGKRIQITIT
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCCCCEEEEEEE
EGKFHQVKRMLLAVDNEVTALTRIAMGPLSLPADLAAGEYRALTDDERTDLDNITR
CCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHCCCCEEECCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]