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The map label for this gene is insI1 [H]

Identifier: 116494074

GI number: 116494074

Start: 517508

End: 518506

Strand: Direct

Name: insI1 [H]

Synonym: LSEI_0516

Alternate gene names: 116494074

Gene position: 517508-518506 (Clockwise)

Preceding gene: 116494073

Following gene: 116494076

Centisome position: 17.87

GC content: 51.15

Gene sequence:

>999_bases
ATGAGTCCGTACACCCATCTTACCTTAAAAGACCGTGAATCGATACTGCTTGGTATCTCTACAGGCAAAACTCTTGATAC
CATCGCCAAAGAGATAGGTCGTTCCAAGAGTACAGTCAGCCGTGAAATTGCACGTAACGGCGGCTGGCGGAGCTATTCGG
CAGCCACCTCTCAGGACCGCTACCGGCGGGTTCGCTTGGCTAGCAGGCGTCCTCGGATCCTCGATCGACCGGGGACTCGT
GACGCTGTCATTCGATATATCACGGTGCTACATTGGTCGCCTGAGCAGATTGCCGGTCGCTTGTCACTAGAAGGCAGTCC
TATTCGCATCAGCTATTCGACTATCTACAGAGGTATCTACCTAGATAATCTCGGCGTTCCATTGAAGAGCCATGGTGCTC
GCGGGCTACCAAGGCTGCTTCGACACCGAGGCAAGACGCGCAAAATCAAAGGCACCATAAATGAACGCCGGGGGCGCTTC
AATGACGTGCCATCAATTCACGACCGACCCCGGTCGGCAGAAAATCGCAGCTGGTTTGGTCACTGGGAAGGCGATACAGT
ACGCGGTAAAACAGGACACTCTGCATTAGTAACATTAGTTGACCGTAAATCACGCTATCTGCTTTCGAAGCGAACGGCCA
ACGCAAAAGCTGACACTGTTAGAGACGTCATGATTGAGCTGCTTGGTGCCTTACCAGCTAACCGAGTAAGAACAGTGACT
CCTGACCGTGGAAGGGAGTTTGCCCGGTACAGGGAGCTGGCAGAACGTCTGAATACAAAGGTCTTCTTTCCTGACCCACA
CGCGCCTCAACAACGAGGAACTAACGAAAACACCAACGGACTGATTAGAGAATACTTTCCCAAGAACACAGACCTAGACC
TTCAGAGCGACCAGGAAATTGAGACTTACATTGAACAACTGAATAATCGACCACGCAAGGTCTTAGGCTGGAAGACGTCA
TCAGAAGTCTTCATGGGTAAAAAGTTGCACTTGAGTTGA

Upstream 100 bases:

>100_bases
ACAATTCAAGTGCAACAAACTAGTCAAATAGAAAAGACTCATAGCCTGAGTCCTTGTAAAATGGAATCACCATAAGACCA
ACTACAAGGAGAACTCGACT

Downstream 100 bases:

>100_bases
CAATTCGTCGCAACAAAAAACCTCCAGCTACCCAGGCTGAAGGCATGCATGAGGGAAGTAAATGTGATTGCAAGGGTGGC
TTGCCTTGCACGATCAGTAT

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATSQDRYRRVRLASRRPRILDRPGTR
DAVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIYLDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRF
NDVPSIHDRPRSAENRSWFGHWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVT
PDRGREFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEIETYIEQLNNRPRKVLGWKTS
SEVFMGKKLHLS

Sequences:

>Translated_332_residues
MSPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATSQDRYRRVRLASRRPRILDRPGTR
DAVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIYLDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRF
NDVPSIHDRPRSAENRSWFGHWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVT
PDRGREFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEIETYIEQLNNRPRKVLGWKTS
SEVFMGKKLHLS
>Mature_331_residues
SPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATSQDRYRRVRLASRRPRILDRPGTRD
AVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIYLDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRFN
DVPSIHDRPRSAENRSWFGHWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVTP
DRGREFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEIETYIEQLNNRPRKVLGWKTSS
EVFMGKKLHLS

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=333, Percent_Identity=41.4414414414414, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1790736, Length=333, Percent_Identity=41.4414414414414, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1787671, Length=333, Percent_Identity=41.4414414414414, Blast_Score=204, Evalue=8e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 38035; Mature: 37904

Theoretical pI: Translated: 11.32; Mature: 11.32

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATSQDR
CCCCCEEEEECCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHH
YRRVRLASRRPRILDRPGTRDAVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCEEEECCCCEEEEHHHHHCEEE
LDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRFNDVPSIHDRPRSAENRSWFG
ECCCCCCHHCCCCCCHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCEEE
HWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVT
ECCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECC
PDRGREFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEI
CCCHHHHHHHHHHHHHHCCEEECCCCCCHHHCCCCCCCCCHHHHHCCCCCCCCCCCCHHH
ETYIEQLNNRPRKVLGWKTSSEVFMGKKLHLS
HHHHHHHCCCCCEEECCCCCCHHHCCEEECCC
>Mature Secondary Structure 
SPYTHLTLKDRESILLGISTGKTLDTIAKEIGRSKSTVSREIARNGGWRSYSAATSQDR
CCCCEEEEECCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHH
YRRVRLASRRPRILDRPGTRDAVIRYITVLHWSPEQIAGRLSLEGSPIRISYSTIYRGIY
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCEEEECCCCEEEEHHHHHCEEE
LDNLGVPLKSHGARGLPRLLRHRGKTRKIKGTINERRGRFNDVPSIHDRPRSAENRSWFG
ECCCCCCHHCCCCCCHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCEEE
HWEGDTVRGKTGHSALVTLVDRKSRYLLSKRTANAKADTVRDVMIELLGALPANRVRTVT
ECCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECC
PDRGREFARYRELAERLNTKVFFPDPHAPQQRGTNENTNGLIREYFPKNTDLDLQSDQEI
CCCHHHHHHHHHHHHHHCCEEECCCCCCHHHCCCCCCCCCHHHHHCCCCCCCCCCCCHHH
ETYIEQLNNRPRKVLGWKTSSEVFMGKKLHLS
HHHHHHHCCCCCEEECCCCCCHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]