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The map label for this gene is spaR [H]

Identifier: 116494021

GI number: 116494021

Start: 467365

End: 468063

Strand: Direct

Name: spaR [H]

Synonym: LSEI_0460

Alternate gene names: 116494021

Gene position: 467365-468063 (Clockwise)

Preceding gene: 116494020

Following gene: 116494022

Centisome position: 16.14

GC content: 46.35

Gene sequence:

>699_bases
ATGCTAAGTGATGAAAGGCTTCGCGGGACAGTGCTTGCAGTAGATGATGATATCGATATTCTAAGGATGTTGAATCGTTA
TTTTGCTGCCCGCCTTCCGACGTTTAACCTAATCACGGCCGAGTCAGGCGCCGGTGCGGTGCAACGGCTGACCAATAATC
CTGATTTGATTTTGCTTGATGTCAACCTACCTGATACTGACGGCTTTTCGTTGGCGGAACGAATTCGGGCGATAACATCG
GTACCAATTATTTTTCTGACCGCACGGGTGACTGATGAGGATAAAGTTCGTGGACTCGGTGCAGGCGGCGACGATTATGT
GACGAAACCGTTCAGTCTTGCTGAACTTGGTGCTCGCATTAAGGCCCACTTTAGACGGGAAAGCCAACCGCAAAAACGTG
CACGGATGGCGATTTTTGATCAGATTCTCATCGACTACCGCGCTAAACAAGTGTCAATTGGCGATCAAGTTTTAAGTCTT
GAAAAGAAGCAATATCAACTAGTTGAGCTGTTAAGCCTGAATCCGGGGCAAGTCTTTAGTCGTGAACAGCTTTATGAAAA
AATATGGGGTTATGACGCTCTTGGAGATGATCGAGTCATTACGGAACATATCCGCCGCATTCGACTTCTTTTTGATCGAT
ATCAAGTAACCTCACCAATCGAAACCGTTTGGGGAGTGGGCTATAAATGGACAAGTTAA

Upstream 100 bases:

>100_bases
CGCCAGCGGGTACAGTAGGCATAACGAAGGAAAATATTCTTTGAAAAGAATGCTAATTAGTTTCCATGAATAAATTGTTG
TCAACAGGGAGGCAAAGCTG

Downstream 100 bases:

>100_bases
AGCAGCGCCTAACGCGTTTGCCAATAAAAAAAGCTTTGCCGCATCTGATTCTGATCGGCACAATTGCCGCTGCATTGGTG
GTGTTGGTCTACTTGCTGAT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MLSDERLRGTVLAVDDDIDILRMLNRYFAARLPTFNLITAESGAGAVQRLTNNPDLILLDVNLPDTDGFSLAERIRAITS
VPIIFLTARVTDEDKVRGLGAGGDDYVTKPFSLAELGARIKAHFRRESQPQKRARMAIFDQILIDYRAKQVSIGDQVLSL
EKKQYQLVELLSLNPGQVFSREQLYEKIWGYDALGDDRVITEHIRRIRLLFDRYQVTSPIETVWGVGYKWTS

Sequences:

>Translated_232_residues
MLSDERLRGTVLAVDDDIDILRMLNRYFAARLPTFNLITAESGAGAVQRLTNNPDLILLDVNLPDTDGFSLAERIRAITS
VPIIFLTARVTDEDKVRGLGAGGDDYVTKPFSLAELGARIKAHFRRESQPQKRARMAIFDQILIDYRAKQVSIGDQVLSL
EKKQYQLVELLSLNPGQVFSREQLYEKIWGYDALGDDRVITEHIRRIRLLFDRYQVTSPIETVWGVGYKWTS
>Mature_232_residues
MLSDERLRGTVLAVDDDIDILRMLNRYFAARLPTFNLITAESGAGAVQRLTNNPDLILLDVNLPDTDGFSLAERIRAITS
VPIIFLTARVTDEDKVRGLGAGGDDYVTKPFSLAELGARIKAHFRRESQPQKRARMAIFDQILIDYRAKQVSIGDQVLSL
EKKQYQLVELLSLNPGQVFSREQLYEKIWGYDALGDDRVITEHIRRIRLLFDRYQVTSPIETVWGVGYKWTS

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=34.9557522123894, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=35.2678571428571, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1786911, Length=189, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1790860, Length=180, Percent_Identity=37.2222222222222, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI87082012, Length=183, Percent_Identity=32.2404371584699, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=35, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786784, Length=180, Percent_Identity=32.7777777777778, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1787375, Length=176, Percent_Identity=36.3636363636364, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789809, Length=234, Percent_Identity=29.0598290598291, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=33.6244541484716, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790863, Length=230, Percent_Identity=29.5652173913043, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787229, Length=205, Percent_Identity=32.1951219512195, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1789402, Length=176, Percent_Identity=32.3863636363636, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=27.2340425531915, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=36.697247706422, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790299, Length=130, Percent_Identity=32.3076923076923, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26402; Mature: 26402

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSDERLRGTVLAVDDDIDILRMLNRYFAARLPTFNLITAESGAGAVQRLTNNPDLILLD
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCEEEEE
VNLPDTDGFSLAERIRAITSVPIIFLTARVTDEDKVRGLGAGGDDYVTKPFSLAELGARI
CCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHH
KAHFRRESQPQKRARMAIFDQILIDYRAKQVSIGDQVLSLEKKQYQLVELLSLNPGQVFS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
REQLYEKIWGYDALGDDRVITEHIRRIRLLFDRYQVTSPIETVWGVGYKWTS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECC
>Mature Secondary Structure
MLSDERLRGTVLAVDDDIDILRMLNRYFAARLPTFNLITAESGAGAVQRLTNNPDLILLD
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCCCEEEEE
VNLPDTDGFSLAERIRAITSVPIIFLTARVTDEDKVRGLGAGGDDYVTKPFSLAELGARI
CCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHH
KAHFRRESQPQKRARMAIFDQILIDYRAKQVSIGDQVLSLEKKQYQLVELLSLNPGQVFS
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH
REQLYEKIWGYDALGDDRVITEHIRRIRLLFDRYQVTSPIETVWGVGYKWTS
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]