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The map label for this gene is cssS [H]

Identifier: 116494022

GI number: 116494022

Start: 468051

End: 469166

Strand: Direct

Name: cssS [H]

Synonym: LSEI_0461

Alternate gene names: 116494022

Gene position: 468051-469166 (Clockwise)

Preceding gene: 116494021

Following gene: 116494023

Centisome position: 16.17

GC content: 46.68

Gene sequence:

>1116_bases
ATGGACAAGTTAAAGCAGCGCCTAACGCGTTTGCCAATAAAAAAAGCTTTGCCGCATCTGATTCTGATCGGCACAATTGC
CGCTGCATTGGTGGTGTTGGTCTACTTGCTGATTTGGCATGTGGTGATGATGCCAATTGCAACTGCCTTGGCCAAGCGCT
GGTTCATTGGCGGGATGAGCTTATACTTTGGTGGGGCAATTATTGGTAGTTTTGCCATGCTATACGCAGTCATGAATGTC
ACAGCCAGACTTTTTTATCGTGTGAAATTGGGGCCGCCGTTGGATCAACTACAATATGGTGTTACTCATATCCAGGCGCA
GGATCTGGACTTCACGCTACAGCCGACATCTGATGATGAACTCGGTCAATTAGTAGCCGCGTTTGAGGAAATGCGCCAAG
CATTACGCAAGGCGCTCGAAACCGCATGGCAATTGGCTGATGATCAAAAACAAGTCAACGCCGCGTTTGCTCATGATCTG
CGGACGCCGCTAACTGTCTTGCAGGGAAATCTGGAATTGTTAACGTTCAATGAAGAAGGCCAGCCACCAGATCAACATTT
ACTAGACGAGATGCAGCGACAGCTTGATCGGATGAACAACTTTATCAAAACGATGAGTCATGTGACCTCTATTCAAAATA
TTCGGTTGAATCATCATGCAATCACAGAAGCACAGGTTGAACAACAGCTTCGTTCAGAAGTGGATAAATTACCAACAGCT
AAGACGATTGGCTGGCAAGTTGATAGCACACGGTTGACAGACAACAAGTTATCGGTTTCGCCAGACGCTATCCTTGAGGT
GTTTGATAACCAGATCAATAATGCCCTCCGGTTTGCTAAGACAAAGGTGGGGATTCATCTCACCTTCAATGACCAGCAAA
TCACCTTGCAGGTTTGCAACGATGGCCAGCCATTAACGGCAACTGAGCAACGCAAGGTCAAATTGCCTTATTTTTCAAAT
GATAAAGCAGCGCATCACCTTGGCGTGGGCATGTATATCTGCAATCAACTATGTGCAGCACATCGCGGTTACTTTAAAAT
TCAAAACCGTGAACATGCGGCGGGTGTTTGTTCGACAGCGGTTTTCGGCTATTTTGACGGCAGCGCCGAAAGCTAA

Upstream 100 bases:

>100_bases
ATGATCGAGTCATTACGGAACATATCCGCCGCATTCGACTTCTTTTTGATCGATATCAAGTAACCTCACCAATCGAAACC
GTTTGGGGAGTGGGCTATAA

Downstream 100 bases:

>100_bases
TGTTACCAACGATGTCAGAAAAATGCTGAAAAAAAGTATTGTTGTCAAAATCTTGTGAATTGCTTGTTAAGGTCTCCTTG
TAAGCTAAAGGAGGCTTTTT

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MDKLKQRLTRLPIKKALPHLILIGTIAAALVVLVYLLIWHVVMMPIATALAKRWFIGGMSLYFGGAIIGSFAMLYAVMNV
TARLFYRVKLGPPLDQLQYGVTHIQAQDLDFTLQPTSDDELGQLVAAFEEMRQALRKALETAWQLADDQKQVNAAFAHDL
RTPLTVLQGNLELLTFNEEGQPPDQHLLDEMQRQLDRMNNFIKTMSHVTSIQNIRLNHHAITEAQVEQQLRSEVDKLPTA
KTIGWQVDSTRLTDNKLSVSPDAILEVFDNQINNALRFAKTKVGIHLTFNDQQITLQVCNDGQPLTATEQRKVKLPYFSN
DKAAHHLGVGMYICNQLCAAHRGYFKIQNREHAAGVCSTAVFGYFDGSAES

Sequences:

>Translated_371_residues
MDKLKQRLTRLPIKKALPHLILIGTIAAALVVLVYLLIWHVVMMPIATALAKRWFIGGMSLYFGGAIIGSFAMLYAVMNV
TARLFYRVKLGPPLDQLQYGVTHIQAQDLDFTLQPTSDDELGQLVAAFEEMRQALRKALETAWQLADDQKQVNAAFAHDL
RTPLTVLQGNLELLTFNEEGQPPDQHLLDEMQRQLDRMNNFIKTMSHVTSIQNIRLNHHAITEAQVEQQLRSEVDKLPTA
KTIGWQVDSTRLTDNKLSVSPDAILEVFDNQINNALRFAKTKVGIHLTFNDQQITLQVCNDGQPLTATEQRKVKLPYFSN
DKAAHHLGVGMYICNQLCAAHRGYFKIQNREHAAGVCSTAVFGYFDGSAES
>Mature_371_residues
MDKLKQRLTRLPIKKALPHLILIGTIAAALVVLVYLLIWHVVMMPIATALAKRWFIGGMSLYFGGAIIGSFAMLYAVMNV
TARLFYRVKLGPPLDQLQYGVTHIQAQDLDFTLQPTSDDELGQLVAAFEEMRQALRKALETAWQLADDQKQVNAAFAHDL
RTPLTVLQGNLELLTFNEEGQPPDQHLLDEMQRQLDRMNNFIKTMSHVTSIQNIRLNHHAITEAQVEQQLRSEVDKLPTA
KTIGWQVDSTRLTDNKLSVSPDAILEVFDNQINNALRFAKTKVGIHLTFNDQQITLQVCNDGQPLTATEQRKVKLPYFSN
DKAAHHLGVGMYICNQLCAAHRGYFKIQNREHAAGVCSTAVFGYFDGSAES

Specific function: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress. Required for the transcription of htrA. Could detect misfolded proteins at the membrane-cell wall interface and then activate CssR by ph

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=26.4957264957265, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1788393, Length=269, Percent_Identity=23.0483271375465, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 41679; Mature: 41679

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLKQRLTRLPIKKALPHLILIGTIAAALVVLVYLLIWHVVMMPIATALAKRWFIGGMS
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LYFGGAIIGSFAMLYAVMNVTARLFYRVKLGPPLDQLQYGVTHIQAQDLDFTLQPTSDDE
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHEEECCCCEEECCCCCHH
LGQLVAAFEEMRQALRKALETAWQLADDQKQVNAAFAHDLRTPLTVLQGNLELLTFNEEG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCC
QPPDQHLLDEMQRQLDRMNNFIKTMSHVTSIQNIRLNHHAITEAQVEQQLRSEVDKLPTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHCCCC
KTIGWQVDSTRLTDNKLSVSPDAILEVFDNQINNALRFAKTKVGIHLTFNDQQITLQVCN
CCCCEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEEEEC
DGQPLTATEQRKVKLPYFSNDKAAHHLGVGMYICNQLCAAHRGYFKIQNREHAAGVCSTA
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHH
VFGYFDGSAES
HHHCCCCCCCC
>Mature Secondary Structure
MDKLKQRLTRLPIKKALPHLILIGTIAAALVVLVYLLIWHVVMMPIATALAKRWFIGGMS
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LYFGGAIIGSFAMLYAVMNVTARLFYRVKLGPPLDQLQYGVTHIQAQDLDFTLQPTSDDE
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHEEECCCCEEECCCCCHH
LGQLVAAFEEMRQALRKALETAWQLADDQKQVNAAFAHDLRTPLTVLQGNLELLTFNEEG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCCEEEEEECCCC
QPPDQHLLDEMQRQLDRMNNFIKTMSHVTSIQNIRLNHHAITEAQVEQQLRSEVDKLPTA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHCCCC
KTIGWQVDSTRLTDNKLSVSPDAILEVFDNQINNALRFAKTKVGIHLTFNDQQITLQVCN
CCCCEEECCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEEEEC
DGQPLTATEQRKVKLPYFSNDKAAHHLGVGMYICNQLCAAHRGYFKIQNREHAAGVCSTA
CCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHH
VFGYFDGSAES
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353931; 9639930; 9384377; 11555295 [H]