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The map label for this gene is glnA [H]

Identifier: 116494002

GI number: 116494002

Start: 443079

End: 444422

Strand: Direct

Name: glnA [H]

Synonym: LSEI_0439

Alternate gene names: 116494002

Gene position: 443079-444422 (Clockwise)

Preceding gene: 116494001

Following gene: 116494003

Centisome position: 15.3

GC content: 49.4

Gene sequence:

>1344_bases
ATGGGCGATGAAGCCGTCATCGAGCAGCTGACAGAGAAGCAAACTGAGCTGATCGAGTTTCTGTATGTGGATTATAACGG
GTTAACGCGTGGTAAGGTGATTCCGCTTGCCAGCTTGAAGGCCAAGCTGGCTGATGGGATTGGGTTGACCAAAGCAACCT
TGAATGTCAGTGAACGTGATACGATTTTGCCGGTGGCGGACATGACGCCTATTGGCGAACTGCGCCTTGTTGGTGATCCG
GCGTCGGCACATGTCCTACCCTATATGCCGCAAGTCGCCACCTTAATGGGGGATATTTACAATCTTGATAAAACCCCGTA
CGCATCTGATCCACGGTCCATTTTGAAAAAAGTCGTCAAGCAGCTTGCTGATGCTGGCTTCACGGTCAAAATGGCCTATG
AAAATGAATTCGAGTTATTTACTGGAGGTAAAGATCATCGCGAACCGGCAATGCCACATGTCGCCTTTTCCACCGAGTCC
ATGGATTTTGCGTATCCATTCATTCTGAAGGCGATCGATCAACTGCAAAAAGTCGGCATTATGCCGAACGCTTATTACCC
AGAAGGTGGCATTGGTCAGCATGAGCTGAGCATGTTGCCGAGTGATCCGGTGACCGCTGCGGACAATGAGGTGATTTACA
AACGGATCATCAAAAATACTGCGAAGGCTTTTGACCTGTATGCCTCATTCGCGCCAAAGCCGCTAGTTGATTCGGCCGGG
TCAGGGGCACACATTCACTTGTCACTTTGGCAAGATAAAGAGGATGCTTTCTTTGATGACAAGGCAGCGATGCAACTATC
CACGATTGGGCAGTATTTTGTCGGCGGTGTTTTGAAACATATTCAAGGTTTGCTGGCATTGACCTGTCCATCGGCTAATT
CATATCAGCGCTTGGCACCTGGTCATTGGAGCAGTGCATACGCCACATATGGTCAAGACAATCGTGAGGCGGCAATTCGC
ATTCCGTCAACCAGTTGGGGTGATCCGGCCAGCTCGATGAACATCGAACTCAAGACAAGTGATGCGACGGCTAATCCTTA
TTTGGCTTTTGCTGGTTTGCTGGCGGCAGGACTTGACGGCATCACAAATCACATGCAACCGGATGGCTATTGCGATGTTG
ATCCAGCCAGTCTCAGTCAAGCTGAGCGCGATGCCAAAGGGATTCAGCTTCTGCCACGCACACTGAGCCAAGCGGTTGAC
GCTTTTGAGGCAGATTCGCTATTCCAGCAAGTATTTGGCAAGCAATTGGTCGATGCCTACGCGAAAATCAAGCGCAAGGA
CGATCAGTATTATGCCAAATTGGCATTGGAAAAAATTGCTGCCTTGCATCGCGAATTGTATTAA

Upstream 100 bases:

>100_bases
ATCATGTAACATTAGGGCCGAACGATACATATGTAACGGTGTTTTTTAACCGGGTGTATCTGAATAATTGGCGAGGGCAT
TCTAAGAGGAGGAAATCAAC

Downstream 100 bases:

>100_bases
ACTATTTTGACATGATCGTTGTCATGAAGGCTGGCAGCGATCACGTTGTATGAAGGCACTTCGCTTTAAAACTTCTAGGA
AAGCGTGAGCTGGTCATTGC

Product: glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MGDEAVIEQLTEKQTELIEFLYVDYNGLTRGKVIPLASLKAKLADGIGLTKATLNVSERDTILPVADMTPIGELRLVGDP
ASAHVLPYMPQVATLMGDIYNLDKTPYASDPRSILKKVVKQLADAGFTVKMAYENEFELFTGGKDHREPAMPHVAFSTES
MDFAYPFILKAIDQLQKVGIMPNAYYPEGGIGQHELSMLPSDPVTAADNEVIYKRIIKNTAKAFDLYASFAPKPLVDSAG
SGAHIHLSLWQDKEDAFFDDKAAMQLSTIGQYFVGGVLKHIQGLLALTCPSANSYQRLAPGHWSSAYATYGQDNREAAIR
IPSTSWGDPASSMNIELKTSDATANPYLAFAGLLAAGLDGITNHMQPDGYCDVDPASLSQAERDAKGIQLLPRTLSQAVD
AFEADSLFQQVFGKQLVDAYAKIKRKDDQYYAKLALEKIAALHRELY

Sequences:

>Translated_447_residues
MGDEAVIEQLTEKQTELIEFLYVDYNGLTRGKVIPLASLKAKLADGIGLTKATLNVSERDTILPVADMTPIGELRLVGDP
ASAHVLPYMPQVATLMGDIYNLDKTPYASDPRSILKKVVKQLADAGFTVKMAYENEFELFTGGKDHREPAMPHVAFSTES
MDFAYPFILKAIDQLQKVGIMPNAYYPEGGIGQHELSMLPSDPVTAADNEVIYKRIIKNTAKAFDLYASFAPKPLVDSAG
SGAHIHLSLWQDKEDAFFDDKAAMQLSTIGQYFVGGVLKHIQGLLALTCPSANSYQRLAPGHWSSAYATYGQDNREAAIR
IPSTSWGDPASSMNIELKTSDATANPYLAFAGLLAAGLDGITNHMQPDGYCDVDPASLSQAERDAKGIQLLPRTLSQAVD
AFEADSLFQQVFGKQLVDAYAKIKRKDDQYYAKLALEKIAALHRELY
>Mature_446_residues
GDEAVIEQLTEKQTELIEFLYVDYNGLTRGKVIPLASLKAKLADGIGLTKATLNVSERDTILPVADMTPIGELRLVGDPA
SAHVLPYMPQVATLMGDIYNLDKTPYASDPRSILKKVVKQLADAGFTVKMAYENEFELFTGGKDHREPAMPHVAFSTESM
DFAYPFILKAIDQLQKVGIMPNAYYPEGGIGQHELSMLPSDPVTAADNEVIYKRIIKNTAKAFDLYASFAPKPLVDSAGS
GAHIHLSLWQDKEDAFFDDKAAMQLSTIGQYFVGGVLKHIQGLLALTCPSANSYQRLAPGHWSSAYATYGQDNREAAIRI
PSTSWGDPASSMNIELKTSDATANPYLAFAGLLAAGLDGITNHMQPDGYCDVDPASLSQAERDAKGIQLLPRTLSQAVDA
FEADSLFQQVFGKQLVDAYAKIKRKDDQYYAKLALEKIAALHRELY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=462, Percent_Identity=24.6753246753247, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1790301, Length=474, Percent_Identity=30.168776371308, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87081870, Length=445, Percent_Identity=26.2921348314607, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 48896; Mature: 48765

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDEAVIEQLTEKQTELIEFLYVDYNGLTRGKVIPLASLKAKLADGIGLTKATLNVSERD
CCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCCC
TILPVADMTPIGELRLVGDPASAHVLPYMPQVATLMGDIYNLDKTPYASDPRSILKKVVK
CEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QLADAGFTVKMAYENEFELFTGGKDHREPAMPHVAFSTESMDFAYPFILKAIDQLQKVGI
HHHCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC
MPNAYYPEGGIGQHELSMLPSDPVTAADNEVIYKRIIKNTAKAFDLYASFAPKPLVDSAG
CCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SGAHIHLSLWQDKEDAFFDDKAAMQLSTIGQYFVGGVLKHIQGLLALTCPSANSYQRLAP
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
GHWSSAYATYGQDNREAAIRIPSTSWGDPASSMNIELKTSDATANPYLAFAGLLAAGLDG
CCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
ITNHMQPDGYCDVDPASLSQAERDAKGIQLLPRTLSQAVDAFEADSLFQQVFGKQLVDAY
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKIKRKDDQYYAKLALEKIAALHRELY
HHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GDEAVIEQLTEKQTELIEFLYVDYNGLTRGKVIPLASLKAKLADGIGLTKATLNVSERD
CHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEHHHHHHHHHCCCCCEEEEECCCCCC
TILPVADMTPIGELRLVGDPASAHVLPYMPQVATLMGDIYNLDKTPYASDPRSILKKVVK
CEEEECCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QLADAGFTVKMAYENEFELFTGGKDHREPAMPHVAFSTESMDFAYPFILKAIDQLQKVGI
HHHCCCCEEEEEECCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCC
MPNAYYPEGGIGQHELSMLPSDPVTAADNEVIYKRIIKNTAKAFDLYASFAPKPLVDSAG
CCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SGAHIHLSLWQDKEDAFFDDKAAMQLSTIGQYFVGGVLKHIQGLLALTCPSANSYQRLAP
CCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
GHWSSAYATYGQDNREAAIRIPSTSWGDPASSMNIELKTSDATANPYLAFAGLLAAGLDG
CCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
ITNHMQPDGYCDVDPASLSQAERDAKGIQLLPRTLSQAVDAFEADSLFQQVFGKQLVDAY
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKIKRKDDQYYAKLALEKIAALHRELY
HHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]