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The map label for this gene is simA

Identifier: 116493936

GI number: 116493936

Start: 369819

End: 371147

Strand: Direct

Name: simA

Synonym: LSEI_0369

Alternate gene names: 116493936

Gene position: 369819-371147 (Clockwise)

Preceding gene: 116493935

Following gene: 116493937

Centisome position: 12.77

GC content: 43.72

Gene sequence:

>1329_bases
ATGGATGATCGGAAGTTTTCAGTTTTAATTGCTGGTGGTGGCAGCACTTATACACCAGGCATTGTATTAACTTTGCTGGA
CCATATTCAGAAGTTTCCGCTACGCAAGCTAAAATTCTATGATATCGATGGTGAACGGCAGCAACGAGTTGCTGATGCTT
GTGAAATCTTGGTGAAGGAACGAGCGCCTGAAGTGGAATTTCTTGCAACGACAGATCCTGAAGAAGCTTTTACCGATGTT
GACTTCGTAATGGCGCAAATTCGCGTTGGCAAGTATGCCATGCGTTCCCTTGATGAGAAGATCCCGCTGAAGCATGGTGT
TGTCGGGCAGGAAACAACAGGACCGGGGGGGATTGCGTACGGCCTTCGTTCTATTCCAGGCGTGATTGGTTTAGTAGATT
ACATGGAAAAGTATTCACCTAATGCGTGGATGCTTAACTACTCAAATCCAGCTGCAATTGTTGCCGAGGCCACTCGCCGC
TTAAGGCCACATTCCCGAATTATTAATATTTGTGATATGCCAATTGGTATTATGGATCGTATGGCACAGATTGTTGGCTT
AAAAGATCGAAATGATTTGGTATTTCGCTATTATGGACTCAATCATTTTGGGTGGTGGACCGATGTCCGTGATAAGACTG
GTAAAGACCTAATGCCAGCACTTAAACAGTATGTTGCCAAGAACGGTTATTGGTTAGGTGACAAAGACAAAGACACTGAA
GCAAGTTGGGTGTCAACTTTTAAGAAGGCTGCTGATGTGTATGCATTGGACCCTTCAACACTGCCAAACACATACTTAAA
ATATTATCTCTATCCAAAATATGTTGTTGAACATTCTGACCCAAATTACACTAGAACAGATGAAGTAGAGGCTTATCGCG
AAAAGCATGTTTTCGATGAATGCGATCGAATTATTGCGGCTGGTACAGCAGCAGACACACATTTTAAGTCTGATGATCAT
GCCACTTATATCGTTGATTTATGCACGGCTATCGCATATGACACGAAGCAACGCATGCTAGCAATTGTGCCTAATGATGG
AGCAATTGAAAACATTGATCCAGAAGCGATGGTTGAGGTGCCATGCTTGTTCGGTGCGAATGGCGCAGAGCGGTTAGCAA
TGGGGAAAGCTGCAACTTTCCAAAAAGGATTGATTACGGAGCAAAACTGCGTTGAAAAGTTGACGGTCGATGCATTTGAA
CAGCAATCGTACACCAAACTTTGGGAAGCGATGAGTCTTTGCAAGATTGTGCCCGATGCTAGTGTTGCTAAAGAAATCTT
GGATGAAATGGTTGTTGCCAACAAAGACTACTGGCCAGAACTGAAGTAG

Upstream 100 bases:

>100_bases
ACCGCTTGCATAAAGCTGCCGTTCGCAGAATACTCAGTATGTACCCGGGGAGAAAAGAAATCGAATTGAAGTTATTATTT
CGGTATAGGAGGACTTATTC

Downstream 100 bases:

>100_bases
CATCCTTAAGGCAAAAATCAAAATAACGCAGCAAGAGCGAGAATTAATCGAAATAAGTTAATTACTCGCTCTTTTCTTTC
AAAACAAAAGTAAGGGCATA

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MDDRKFSVLIAGGGSTYTPGIVLTLLDHIQKFPLRKLKFYDIDGERQQRVADACEILVKERAPEVEFLATTDPEEAFTDV
DFVMAQIRVGKYAMRSLDEKIPLKHGVVGQETTGPGGIAYGLRSIPGVIGLVDYMEKYSPNAWMLNYSNPAAIVAEATRR
LRPHSRIINICDMPIGIMDRMAQIVGLKDRNDLVFRYYGLNHFGWWTDVRDKTGKDLMPALKQYVAKNGYWLGDKDKDTE
ASWVSTFKKAADVYALDPSTLPNTYLKYYLYPKYVVEHSDPNYTRTDEVEAYREKHVFDECDRIIAAGTAADTHFKSDDH
ATYIVDLCTAIAYDTKQRMLAIVPNDGAIENIDPEAMVEVPCLFGANGAERLAMGKAATFQKGLITEQNCVEKLTVDAFE
QQSYTKLWEAMSLCKIVPDASVAKEILDEMVVANKDYWPELK

Sequences:

>Translated_442_residues
MDDRKFSVLIAGGGSTYTPGIVLTLLDHIQKFPLRKLKFYDIDGERQQRVADACEILVKERAPEVEFLATTDPEEAFTDV
DFVMAQIRVGKYAMRSLDEKIPLKHGVVGQETTGPGGIAYGLRSIPGVIGLVDYMEKYSPNAWMLNYSNPAAIVAEATRR
LRPHSRIINICDMPIGIMDRMAQIVGLKDRNDLVFRYYGLNHFGWWTDVRDKTGKDLMPALKQYVAKNGYWLGDKDKDTE
ASWVSTFKKAADVYALDPSTLPNTYLKYYLYPKYVVEHSDPNYTRTDEVEAYREKHVFDECDRIIAAGTAADTHFKSDDH
ATYIVDLCTAIAYDTKQRMLAIVPNDGAIENIDPEAMVEVPCLFGANGAERLAMGKAATFQKGLITEQNCVEKLTVDAFE
QQSYTKLWEAMSLCKIVPDASVAKEILDEMVVANKDYWPELK
>Mature_442_residues
MDDRKFSVLIAGGGSTYTPGIVLTLLDHIQKFPLRKLKFYDIDGERQQRVADACEILVKERAPEVEFLATTDPEEAFTDV
DFVMAQIRVGKYAMRSLDEKIPLKHGVVGQETTGPGGIAYGLRSIPGVIGLVDYMEKYSPNAWMLNYSNPAAIVAEATRR
LRPHSRIINICDMPIGIMDRMAQIVGLKDRNDLVFRYYGLNHFGWWTDVRDKTGKDLMPALKQYVAKNGYWLGDKDKDTE
ASWVSTFKKAADVYALDPSTLPNTYLKYYLYPKYVVEHSDPNYTRTDEVEAYREKHVFDECDRIIAAGTAADTHFKSDDH
ATYIVDLCTAIAYDTKQRMLAIVPNDGAIENIDPEAMVEVPCLFGANGAERLAMGKAATFQKGLITEQNCVEKLTVDAFE
QQSYTKLWEAMSLCKIVPDASVAKEILDEMVVANKDYWPELK

Specific function: Is probably involved in the catabolism of alpha- glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D-glucosides including the five isomeric derivatives of sucrose,

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family

Homologues:

Organism=Escherichia coli, GI1788029, Length=449, Percent_Identity=28.2850779510022, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1790560, Length=471, Percent_Identity=23.1422505307856, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAGL1_LACC3 (Q03C44)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805670.1
- ProteinModelPortal:   Q03C44
- SMR:   Q03C44
- STRING:   Q03C44
- GeneID:   4421568
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0369
- eggNOG:   COG1486
- HOGENOM:   HBG511715
- OMA:   IKFYDND
- PhylomeDB:   Q03C44
- ProtClustDB:   CLSK880767
- BioCyc:   LCAS321967:LSEI_0369-MONOMER
- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00732

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =3.2.1.122

Molecular weight: Translated: 49842; Mature: 49842

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: ACT_SITE 172-172 ACT_SITE 265-265 BINDING 95-95 BINDING 149-149 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDRKFSVLIAGGGSTYTPGIVLTLLDHIQKFPLRKLKFYDIDGERQQRVADACEILVKE
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHH
RAPEVEFLATTDPEEAFTDVDFVMAQIRVGKYAMRSLDEKIPLKHGVVGQETTGPGGIAY
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
GLRSIPGVIGLVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPHSRIINICDMPIGIMDR
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
MAQIVGLKDRNDLVFRYYGLNHFGWWTDVRDKTGKDLMPALKQYVAKNGYWLGDKDKDTE
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
ASWVSTFKKAADVYALDPSTLPNTYLKYYLYPKYVVEHSDPNYTRTDEVEAYREKHVFDE
HHHHHHHHHHCCEEEECCCCCCCHHHEEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CDRIIAAGTAADTHFKSDDHATYIVDLCTAIAYDTKQRMLAIVPNDGAIENIDPEAMVEV
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHEEE
PCLFGANGAERLAMGKAATFQKGLITEQNCVEKLTVDAFEQQSYTKLWEAMSLCKIVPDA
CCEECCCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SVAKEILDEMVVANKDYWPELK
HHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDDRKFSVLIAGGGSTYTPGIVLTLLDHIQKFPLRKLKFYDIDGERQQRVADACEILVKE
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHEEEEECCCHHHHHHHHHHHHHHHH
RAPEVEFLATTDPEEAFTDVDFVMAQIRVGKYAMRSLDEKIPLKHGVVGQETTGPGGIAY
CCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
GLRSIPGVIGLVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPHSRIINICDMPIGIMDR
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHH
MAQIVGLKDRNDLVFRYYGLNHFGWWTDVRDKTGKDLMPALKQYVAKNGYWLGDKDKDTE
HHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCH
ASWVSTFKKAADVYALDPSTLPNTYLKYYLYPKYVVEHSDPNYTRTDEVEAYREKHVFDE
HHHHHHHHHHCCEEEECCCCCCCHHHEEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CDRIIAAGTAADTHFKSDDHATYIVDLCTAIAYDTKQRMLAIVPNDGAIENIDPEAMVEV
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHEEE
PCLFGANGAERLAMGKAATFQKGLITEQNCVEKLTVDAFEQQSYTKLWEAMSLCKIVPDA
CCEECCCCHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SVAKEILDEMVVANKDYWPELK
HHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA