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The map label for this gene is tnpR [H]

Identifier: 116493911

GI number: 116493911

Start: 344092

End: 344661

Strand: Direct

Name: tnpR [H]

Synonym: LSEI_0344

Alternate gene names: 116493911

Gene position: 344092-344661 (Clockwise)

Preceding gene: 116493907

Following gene: 116493914

Centisome position: 11.88

GC content: 38.42

Gene sequence:

>570_bases
ATGAAATATGGCTATGCGCGGGTCAGTACCACTGATCAAAAATTAGCAAATCAAATTGAGTTACTAAAATTGGCGGGAGC
AGAAAAAGTCTTTCAAGAAAAATTTACTGGGACGACTACTGCACGACCGGAGTTTCAAAAACTATTACAAGCTCTAAAAA
CAGATGATACTTTAATTGTGACTAAGTTGGATCGGTTTGCGCGGAATACACGTGAGGCTTTAACTATCATCCAAGAGCTG
TTTAACCGGAACATCAAAGTTAATATTTTGAACATGGGCTTAATTGATAATACACCGACTGGCCAATTAGTCTTCACAAT
ATTTAGTGCTTTTGCGCAGTTTGAACGAGATATGATTGTCACGCGCACGCAAGAAGGCAAGTTGTATGCCAAACAACATG
ATCCGTTATTTCGGGAGGGTAGACCGAAAACTTATTCTGATGAACAGATCAGATTTGCCTACGAATTACGAAAACAGGGC
ATGACCTATAAAATGATTGAACGAAAGACGGGGATTAGTAAACGCACGCAACAGCGACGATTTAAATTAGTTAAAAACAA
CATATTTTAA

Upstream 100 bases:

>100_bases
CAGGTCACAACTTAACAGTTGTGGCCTCTTTTTTGATGCTGTATAATCAAAGGGCTAGAACCGTGTCATTACTTAACGAC
ATAAGTAAGGAGATAAAAAG

Downstream 100 bases:

>100_bases
AACTGTTTACACCATGCCAAAAGGGGCTTCGAATACGAGGCCCCTTTTAGATTTAGCTAGGGTGCGTCTTCAAACTACTT
AGACATTAAGAATATCGCCG

Product: site-specific recombinase, DNA invertase Pin related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MKYGYARVSTTDQKLANQIELLKLAGAEKVFQEKFTGTTTARPEFQKLLQALKTDDTLIVTKLDRFARNTREALTIIQEL
FNRNIKVNILNMGLIDNTPTGQLVFTIFSAFAQFERDMIVTRTQEGKLYAKQHDPLFREGRPKTYSDEQIRFAYELRKQG
MTYKMIERKTGISKRTQQRRFKLVKNNIF

Sequences:

>Translated_189_residues
MKYGYARVSTTDQKLANQIELLKLAGAEKVFQEKFTGTTTARPEFQKLLQALKTDDTLIVTKLDRFARNTREALTIIQEL
FNRNIKVNILNMGLIDNTPTGQLVFTIFSAFAQFERDMIVTRTQEGKLYAKQHDPLFREGRPKTYSDEQIRFAYELRKQG
MTYKMIERKTGISKRTQQRRFKLVKNNIF
>Mature_189_residues
MKYGYARVSTTDQKLANQIELLKLAGAEKVFQEKFTGTTTARPEFQKLLQALKTDDTLIVTKLDRFARNTREALTIIQEL
FNRNIKVNILNMGLIDNTPTGQLVFTIFSAFAQFERDMIVTRTQEGKLYAKQHDPLFREGRPKTYSDEQIRFAYELRKQG
MTYKMIERKTGISKRTQQRRFKLVKNNIF

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787638, Length=181, Percent_Identity=35.3591160220994, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787827, Length=181, Percent_Identity=35.3591160220994, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1787404, Length=126, Percent_Identity=41.2698412698413, Blast_Score=104, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22128; Mature: 22128

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYGYARVSTTDQKLANQIELLKLAGAEKVFQEKFTGTTTARPEFQKLLQALKTDDTLIV
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
TKLDRFARNTREALTIIQELFNRNIKVNILNMGLIDNTPTGQLVFTIFSAFAQFERDMIV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEE
TRTQEGKLYAKQHDPLFREGRPKTYSDEQIRFAYELRKQGMTYKMIERKTGISKRTQQRR
EECCCCCEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
FKLVKNNIF
HHHHHHCCC
>Mature Secondary Structure
MKYGYARVSTTDQKLANQIELLKLAGAEKVFQEKFTGTTTARPEFQKLLQALKTDDTLIV
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
TKLDRFARNTREALTIIQELFNRNIKVNILNMGLIDNTPTGQLVFTIFSAFAQFERDMIV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEE
TRTQEGKLYAKQHDPLFREGRPKTYSDEQIRFAYELRKQGMTYKMIERKTGISKRTQQRR
EECCCCCEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
FKLVKNNIF
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]