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The map label for this gene is adaB [H]

Identifier: 116493716

GI number: 116493716

Start: 145156

End: 145647

Strand: Direct

Name: adaB [H]

Synonym: LSEI_0144

Alternate gene names: 116493716

Gene position: 145156-145647 (Clockwise)

Preceding gene: 116493714

Following gene: 116493717

Centisome position: 5.01

GC content: 53.05

Gene sequence:

>492_bases
ATGCAATACACACGGTTAGCAATGCCCAATCACGCCTATTTTATCGTGGCCGATCAAGGGAAACTGGCCTTCGTCGGGTC
ACCGGATGCGCCGCTTAGCGAGGCGGCTGATTATGTCAGCGGTTCACTTGAATTTAATCAAGAAGAGATGTTGCCGTTTC
AAAACGCCATTGATGATTATCTTACTGGCAAGGCGCAAACCATCACAGTACCGGTGACTTACCATGGGACACCGCTGCAA
GAAGCCGTTTGGCAGTATCTGCAGACGATTCCTTATGGAGAGACCCGAACGTACGCACAGGTGGCAGCCGCAGTCGGTCA
TTCGCACGCCTTCCAAGCAGTCGGCTCTGCGGTCGGAAAGAATCCCGTTATGATCGCTGTGCCTTGTCATCGCGTCCTGC
GCAAAGATGGTGGTTTGGGTGGCTTTCGTGGCGGTTTGCCAATGAAGCGCGACTTGTTGGCACTTGAACAAGGGCAGCGG
CCCATTTTTTAA

Upstream 100 bases:

>100_bases
AAAGATGCTAAAAAATGGTGTAATATTAAGAAGGCTTTTAAACCCTCTGCCATGGTTGTGAACGCGCTTTCTATAAACAG
CGCAGATAAGGAGTCAAAGA

Downstream 100 bases:

>100_bases
AGATGCTTTTTCACCGAGGCATCTTGTATACTGGTGAGTGGACAAATTGGAAAGGAAGTTTAACACATGAAGATTATTGC
TTACGGTGCACGCGTTGACG

Product: methylated DNA-protein cysteine methyltransferase

Products: NA

Alternate protein names: O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MQYTRLAMPNHAYFIVADQGKLAFVGSPDAPLSEAADYVSGSLEFNQEEMLPFQNAIDDYLTGKAQTITVPVTYHGTPLQ
EAVWQYLQTIPYGETRTYAQVAAAVGHSHAFQAVGSAVGKNPVMIAVPCHRVLRKDGGLGGFRGGLPMKRDLLALEQGQR
PIF

Sequences:

>Translated_163_residues
MQYTRLAMPNHAYFIVADQGKLAFVGSPDAPLSEAADYVSGSLEFNQEEMLPFQNAIDDYLTGKAQTITVPVTYHGTPLQ
EAVWQYLQTIPYGETRTYAQVAAAVGHSHAFQAVGSAVGKNPVMIAVPCHRVLRKDGGLGGFRGGLPMKRDLLALEQGQR
PIF
>Mature_163_residues
MQYTRLAMPNHAYFIVADQGKLAFVGSPDAPLSEAADYVSGSLEFNQEEMLPFQNAIDDYLTGKAQTITVPVTYHGTPLQ
EAVWQYLQTIPYGETRTYAQVAAAVGHSHAFQAVGSAVGKNPVMIAVPCHRVLRKDGGLGGFRGGLPMKRDLLALEQGQR
PIF

Specific function: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction:the enzyme is irreversibly inactivated [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=139, Percent_Identity=34.5323741007194, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1788542, Length=142, Percent_Identity=41.5492957746479, Blast_Score=94, Evalue=4e-21,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=40.9523809523809, Blast_Score=86, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=91, Percent_Identity=41.7582417582418, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533070, Length=91, Percent_Identity=41.7582417582418, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17738; Mature: 17738

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYTRLAMPNHAYFIVADQGKLAFVGSPDAPLSEAADYVSGSLEFNQEEMLPFQNAIDDY
CCCCEECCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCCCCHHHCCCHHHHHHHH
LTGKAQTITVPVTYHGTPLQEAVWQYLQTIPYGETRTYAQVAAAVGHSHAFQAVGSAVGK
HCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC
NPVMIAVPCHRVLRKDGGLGGFRGGLPMKRDLLALEQGQRPIF
CCEEEEECHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQYTRLAMPNHAYFIVADQGKLAFVGSPDAPLSEAADYVSGSLEFNQEEMLPFQNAIDDY
CCCCEECCCCCEEEEEECCCCEEEECCCCCCHHHHHHHHCCCCCCCHHHCCCHHHHHHHH
LTGKAQTITVPVTYHGTPLQEAVWQYLQTIPYGETRTYAQVAAAVGHSHAFQAVGSAVGK
HCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCC
NPVMIAVPCHRVLRKDGGLGGFRGGLPMKRDLLALEQGQRPIF
CCEEEEECHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2120677; 9384377; 1744039 [H]