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The map label for this gene is yclM [H]

Identifier: 116493671

GI number: 116493671

Start: 93067

End: 94446

Strand: Direct

Name: yclM [H]

Synonym: LSEI_0099

Alternate gene names: 116493671

Gene position: 93067-94446 (Clockwise)

Preceding gene: 116493665

Following gene: 116493672

Centisome position: 3.21

GC content: 48.7

Gene sequence:

>1380_bases
GTGCGTGTCGTAAAATTTGGCGGTTCATCGCTAGCAAATGGTTCACAATTCGCCAAGGTCGTGGCGATCATGAAGGCTAA
CCCAGCTCGGCAGGTGATCGTGACGAGTGCGCCAGGTAAACGGCACCCGGGAGACACCAAGGTGACCGATTTATTGATTC
AGTATGCACAGGCAGTGATGCGGCATGAAGCTGTGGCTGCCATTGTTGCAGAAATCGTTGCTCGATATCAGGCAATCGCC
GATGATTTTGAGCTCGAAGCATCGATCATGGCCGCGATTGAAGAAGAGCTAGACCAGTTACCAAAGTTGACTTTCAAGAA
CAATGATTATTTAATGGCAGGCTTTAAGGCGCGCGGTGAAAAGTTGAACGCGTTTTTAATGACCGCGGTGTTGAATCATG
AGGGGATTGAAGCTACCTATGGCGAACCGAAGTCGTTAGGAATTGTGGTCACAGATCATCCCAATGATGCCGAAGTTTCA
CGTGAAACCTATCGCAACCTCGCTACATTTCGACGACCAATCGGGGTTTTGGTTGTACCGGGATTTTATGGCTATACCAG
CGATGACGATATTGCCACCTTTTCTCGTGGTGGCTCCGATATCACTGGGGCGATTTTGGCTAACGGTTTTCATGCCGACT
TATATGAAAACTTTACCGATGTCGATGGCGTAGCGGCGGCTGACCCGCGGGTAGTGGCGAATCCGCAAATGATTCGTGAA
ATGACCTATCGGGAAATGCGGGAGTTGTCTTATGGCGGCTTTGGGGTTTTTCATGATGAAGCGATTCTGCCAGCGATTGC
TGGACAGGTGCCGATTAATCTTAAAAACACCAATCATCCTGCTGCTCCCGGGACCATGATCGTCCCAGAACAGCATTTTG
TCCCGAAACATGCCATTACCGGGATTGTAAGTTCCCAACATTTTGGGGCGATCTACCTGCATCGATATCTGTTGAATAAG
GAAGTTGGCTTCACTCTGAAGATCCTTCAGGTGTTAGCCCACAACGGCATTAGTTATGAGCACATGCCCAGCGGAATTGA
TGATCTCACCATTATTTTAGATGATCGCCAGCTAACTGAGGCCAAACGCAAGACAATTTGTGCCGAGATTCAAGCGGCGG
TTCATCCAGATGTGCTGAAGTGGATTGATGACTATGCGATCATCATGGTCGTTGGTGAAGGGATGGCGCAACGAACCGGA
TTGATTCAAACCATTCTACAACCGCTCGCAACCGCCGGTGTTCATGTCTCAATGATTAATGAGGGGGCCTCACAGATTTC
TCTGATGCTGGGAACCTCACGTGAGTCGGCCAATCGCGCTGTTAAAACGATCTACAATGCTTTTTTTATACCGATTAATC
AACGTTTGGAGGTGCACTAA

Upstream 100 bases:

>100_bases
TAGCGACATGCTGGGGCTTGTGTTTCTGGGAGAGTCTGTTAAAGTACAGGATAAAATGACAAAGTTCTCATTTGTCGTGA
CGTCTAGGAGGCAGTTTATC

Downstream 100 bases:

>100_bases
CATGGTGACTTTGCAAAAGGTTCATGGTTCCGAAAATAGTTTCTTTCTTTTCGATGAAACGCAGTTAACTCAGCCGTTGA
CAAACACCCAATTAGCAACC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MRVVKFGGSSLANGSQFAKVVAIMKANPARQVIVTSAPGKRHPGDTKVTDLLIQYAQAVMRHEAVAAIVAEIVARYQAIA
DDFELEASIMAAIEEELDQLPKLTFKNNDYLMAGFKARGEKLNAFLMTAVLNHEGIEATYGEPKSLGIVVTDHPNDAEVS
RETYRNLATFRRPIGVLVVPGFYGYTSDDDIATFSRGGSDITGAILANGFHADLYENFTDVDGVAAADPRVVANPQMIRE
MTYREMRELSYGGFGVFHDEAILPAIAGQVPINLKNTNHPAAPGTMIVPEQHFVPKHAITGIVSSQHFGAIYLHRYLLNK
EVGFTLKILQVLAHNGISYEHMPSGIDDLTIILDDRQLTEAKRKTICAEIQAAVHPDVLKWIDDYAIIMVVGEGMAQRTG
LIQTILQPLATAGVHVSMINEGASQISLMLGTSRESANRAVKTIYNAFFIPINQRLEVH

Sequences:

>Translated_459_residues
MRVVKFGGSSLANGSQFAKVVAIMKANPARQVIVTSAPGKRHPGDTKVTDLLIQYAQAVMRHEAVAAIVAEIVARYQAIA
DDFELEASIMAAIEEELDQLPKLTFKNNDYLMAGFKARGEKLNAFLMTAVLNHEGIEATYGEPKSLGIVVTDHPNDAEVS
RETYRNLATFRRPIGVLVVPGFYGYTSDDDIATFSRGGSDITGAILANGFHADLYENFTDVDGVAAADPRVVANPQMIRE
MTYREMRELSYGGFGVFHDEAILPAIAGQVPINLKNTNHPAAPGTMIVPEQHFVPKHAITGIVSSQHFGAIYLHRYLLNK
EVGFTLKILQVLAHNGISYEHMPSGIDDLTIILDDRQLTEAKRKTICAEIQAAVHPDVLKWIDDYAIIMVVGEGMAQRTG
LIQTILQPLATAGVHVSMINEGASQISLMLGTSRESANRAVKTIYNAFFIPINQRLEVH
>Mature_459_residues
MRVVKFGGSSLANGSQFAKVVAIMKANPARQVIVTSAPGKRHPGDTKVTDLLIQYAQAVMRHEAVAAIVAEIVARYQAIA
DDFELEASIMAAIEEELDQLPKLTFKNNDYLMAGFKARGEKLNAFLMTAVLNHEGIEATYGEPKSLGIVVTDHPNDAEVS
RETYRNLATFRRPIGVLVVPGFYGYTSDDDIATFSRGGSDITGAILANGFHADLYENFTDVDGVAAADPRVVANPQMIRE
MTYREMRELSYGGFGVFHDEAILPAIAGQVPINLKNTNHPAAPGTMIVPEQHFVPKHAITGIVSSQHFGAIYLHRYLLNK
EVGFTLKILQVLAHNGISYEHMPSGIDDLTIILDDRQLTEAKRKTICAEIQAAVHPDVLKWIDDYAIIMVVGEGMAQRTG
LIQTILQPLATAGVHVSMINEGASQISLMLGTSRESANRAVKTIYNAFFIPINQRLEVH

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=466, Percent_Identity=27.6824034334764, Blast_Score=151, Evalue=7e-38,
Organism=Escherichia coli, GI1790455, Length=469, Percent_Identity=23.8805970149254, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790376, Length=292, Percent_Identity=27.0547945205479, Blast_Score=73, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320893, Length=512, Percent_Identity=23.4375, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 50208; Mature: 50208

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVKFGGSSLANGSQFAKVVAIMKANPARQVIVTSAPGKRHPGDTKVTDLLIQYAQAVM
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
RHEAVAAIVAEIVARYQAIADDFELEASIMAAIEEELDQLPKLTFKNNDYLMAGFKARGE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECHHCCC
KLNAFLMTAVLNHEGIEATYGEPKSLGIVVTDHPNDAEVSRETYRNLATFRRPIGVLVVP
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEC
GFYGYTSDDDIATFSRGGSDITGAILANGFHADLYENFTDVDGVAAADPRVVANPQMIRE
CCCCCCCCCCCHHHCCCCCCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCEECCHHHHHH
MTYREMRELSYGGFGVFHDEAILPAIAGQVPINLKNTNHPAAPGTMIVPEQHFVPKHAIT
HHHHHHHHCCCCCCEEECCCHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHH
GIVSSQHFGAIYLHRYLLNKEVGFTLKILQVLAHNGISYEHMPSGIDDLTIILDDRQLTE
HHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCCCCCCCEEEEECCHHHHH
AKRKTICAEIQAAVHPDVLKWIDDYAIIMVVGEGMAQRTGLIQTILQPLATAGVHVSMIN
HHHHHHHHHHHHHCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEEC
EGASQISLMLGTSRESANRAVKTIYNAFFIPINQRLEVH
CCCHHEEEEEECCCHHHHHHHHHHHHHHEEEECCCEECC
>Mature Secondary Structure
MRVVKFGGSSLANGSQFAKVVAIMKANPARQVIVTSAPGKRHPGDTKVTDLLIQYAQAVM
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
RHEAVAAIVAEIVARYQAIADDFELEASIMAAIEEELDQLPKLTFKNNDYLMAGFKARGE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECHHCCC
KLNAFLMTAVLNHEGIEATYGEPKSLGIVVTDHPNDAEVSRETYRNLATFRRPIGVLVVP
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEC
GFYGYTSDDDIATFSRGGSDITGAILANGFHADLYENFTDVDGVAAADPRVVANPQMIRE
CCCCCCCCCCCHHHCCCCCCCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCEECCHHHHHH
MTYREMRELSYGGFGVFHDEAILPAIAGQVPINLKNTNHPAAPGTMIVPEQHFVPKHAIT
HHHHHHHHCCCCCCEEECCCHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCCCCHHHHH
GIVSSQHFGAIYLHRYLLNKEVGFTLKILQVLAHNGISYEHMPSGIDDLTIILDDRQLTE
HHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCCCCCCCEEEEECCHHHHH
AKRKTICAEIQAAVHPDVLKWIDDYAIIMVVGEGMAQRTGLIQTILQPLATAGVHVSMIN
HHHHHHHHHHHHHCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEEC
EGASQISLMLGTSRESANRAVKTIYNAFFIPINQRLEVH
CCCHHEEEEEECCCHHHHHHHHHHHHHHEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]