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The map label for this gene is dapF [H]

Identifier: 116493672

GI number: 116493672

Start: 94448

End: 95455

Strand: Direct

Name: dapF [H]

Synonym: LSEI_0100

Alternate gene names: 116493672

Gene position: 94448-95455 (Clockwise)

Preceding gene: 116493671

Following gene: 116493673

Centisome position: 3.26

GC content: 50.4

Gene sequence:

>1008_bases
ATGGTGACTTTGCAAAAGGTTCATGGTTCCGAAAATAGTTTCTTTCTTTTCGATGAAACGCAGTTAACTCAGCCGTTGAC
AAACACCCAATTAGCAACCGTGACCCAACAGCTAACGAACGCTGAAACAGGTTTGCTTGGCGGTGCGGACGGGGTCTTGG
CAATCACGGCGGCGCCTGGAACGGCTGGCAAAATGACGGTGGTCAACACTGATGGCAGTTTTGCTAAATTGTGTGGCAAC
GGTCTGCGCACGGTTGCCCGTTATCTGAGTGAAAAAACTGGGCAAGCGACGTTCAAGGTGCACACGGATTTTGCTGATCT
TGCCGTTGCTCGTCAGCCGCAACTGGCAGAAGGCGTACCGGCCTACTCAGTGGAAATTTCGCCGGTTAGTTTTGCGCCTC
AGGACTTGCCGTTTGCCAATCTTGGCGTTGACCGAATTGTGGATACTGTTTTGCCAGCGATTCATCCGAGCTTGAAATTC
ACCGCCATTGCTGTGCCCAACCCGCACTTAATTGCTTTCGTCTCACCTGAGGTATTAGCTGGGTCCGATCTCGAACGGAT
TGGCAAGATGCTGAATGCCAAAAATCCTTATTTTCCGGAAGGGGTCAACGTGACTTTTGCCGAAATCTTAGATAAGGATA
CCTTATTTGCTCGGACGTATGAGCGGGGTGTCGGGTTCACCAATGCATGTGGCACAGGCATGTCAGCAACGACCTTGGCT
TTTATTTTGACGCATCCAGAGGAAGCTGCTTTTGATCGGGTCAATACCGTGTATAATCCAGGAGGAATGGTCAAAACAAT
TGTCCATCATGACGGCGAGCGTTACTGGATTGAATTGATCGGGAATGCAACGGTCACGGCGACGATTACCTTGCCAGACA
CGGCGTTACTCGCAGGCGACTGGCAACAAGCTGAGATTCACGCGACTGGCGAACAGGCTGAGTATGAACGATTTATTGAC
GCACTGCCGCATCGGAAGTTAGCAAACACATTGACAGAAGGAGTTTAG

Upstream 100 bases:

>100_bases
CTGATGCTGGGAACCTCACGTGAGTCGGCCAATCGCGCTGTTAAAACGATCTACAATGCTTTTTTTATACCGATTAATCA
ACGTTTGGAGGTGCACTAAC

Downstream 100 bases:

>100_bases
ATCATGAGAGGTTATCACGTAGCAGTTGTTGGGACTACTGGAGCTGTTGGCACCCGGATGTTAAAAATGGTGGCAGAAAG
TAAACTGCCGATTGCGTCTG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MVTLQKVHGSENSFFLFDETQLTQPLTNTQLATVTQQLTNAETGLLGGADGVLAITAAPGTAGKMTVVNTDGSFAKLCGN
GLRTVARYLSEKTGQATFKVHTDFADLAVARQPQLAEGVPAYSVEISPVSFAPQDLPFANLGVDRIVDTVLPAIHPSLKF
TAIAVPNPHLIAFVSPEVLAGSDLERIGKMLNAKNPYFPEGVNVTFAEILDKDTLFARTYERGVGFTNACGTGMSATTLA
FILTHPEEAAFDRVNTVYNPGGMVKTIVHHDGERYWIELIGNATVTATITLPDTALLAGDWQQAEIHATGEQAEYERFID
ALPHRKLANTLTEGV

Sequences:

>Translated_335_residues
MVTLQKVHGSENSFFLFDETQLTQPLTNTQLATVTQQLTNAETGLLGGADGVLAITAAPGTAGKMTVVNTDGSFAKLCGN
GLRTVARYLSEKTGQATFKVHTDFADLAVARQPQLAEGVPAYSVEISPVSFAPQDLPFANLGVDRIVDTVLPAIHPSLKF
TAIAVPNPHLIAFVSPEVLAGSDLERIGKMLNAKNPYFPEGVNVTFAEILDKDTLFARTYERGVGFTNACGTGMSATTLA
FILTHPEEAAFDRVNTVYNPGGMVKTIVHHDGERYWIELIGNATVTATITLPDTALLAGDWQQAEIHATGEQAEYERFID
ALPHRKLANTLTEGV
>Mature_335_residues
MVTLQKVHGSENSFFLFDETQLTQPLTNTQLATVTQQLTNAETGLLGGADGVLAITAAPGTAGKMTVVNTDGSFAKLCGN
GLRTVARYLSEKTGQATFKVHTDFADLAVARQPQLAEGVPAYSVEISPVSFAPQDLPFANLGVDRIVDTVLPAIHPSLKF
TAIAVPNPHLIAFVSPEVLAGSDLERIGKMLNAKNPYFPEGVNVTFAEILDKDTLFARTYERGVGFTNACGTGMSATTLA
FILTHPEEAAFDRVNTVYNPGGMVKTIVHHDGERYWIELIGNATVTATITLPDTALLAGDWQQAEIHATGEQAEYERFID
ALPHRKLANTLTEGV

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=241, Percent_Identity=24.896265560166, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 35928; Mature: 35928

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLQKVHGSENSFFLFDETQLTQPLTNTQLATVTQQLTNAETGLLGGADGVLAITAAPG
CEEEEEECCCCCCEEEEECHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
TAGKMTVVNTDGSFAKLCGNGLRTVARYLSEKTGQATFKVHTDFADLAVARQPQLAEGVP
CCCEEEEEECCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCHHCCCC
AYSVEISPVSFAPQDLPFANLGVDRIVDTVLPAIHPSLKFTAIAVPNPHLIAFVSPEVLA
CEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCHHHC
GSDLERIGKMLNAKNPYFPEGVNVTFAEILDKDTLFARTYERGVGFTNACGTGMSATTLA
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHEEE
FILTHPEEAAFDRVNTVYNPGGMVKTIVHHDGERYWIELIGNATVTATITLPDTALLAGD
EEEECCCHHHHHHHHHEECCCCCEEEEECCCCCEEEEEEECCCEEEEEEEECCCEEEECC
WQQAEIHATGEQAEYERFIDALPHRKLANTLTEGV
CCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MVTLQKVHGSENSFFLFDETQLTQPLTNTQLATVTQQLTNAETGLLGGADGVLAITAAPG
CEEEEEECCCCCCEEEEECHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCC
TAGKMTVVNTDGSFAKLCGNGLRTVARYLSEKTGQATFKVHTDFADLAVARQPQLAEGVP
CCCEEEEEECCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCHHCCCC
AYSVEISPVSFAPQDLPFANLGVDRIVDTVLPAIHPSLKFTAIAVPNPHLIAFVSPEVLA
CEEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCHHHC
GSDLERIGKMLNAKNPYFPEGVNVTFAEILDKDTLFARTYERGVGFTNACGTGMSATTLA
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHEEE
FILTHPEEAAFDRVNTVYNPGGMVKTIVHHDGERYWIELIGNATVTATITLPDTALLAGD
EEEECCCHHHHHHHHHEECCCCCEEEEECCCCCEEEEEEECCCEEEEEEEECCCEEEECC
WQQAEIHATGEQAEYERFIDALPHRKLANTLTEGV
CCEEEEEECCCHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA